Determination of Total Antioxidant Capacity, Radical Scavenging Activity and Fatty Acid Composition of Ultrasonic Assisted Ethanolic Extraction of Oat (Avena sativa L.) Grains
Yıl 2022,
, 326 - 334, 30.12.2022
Ümit Erdoğan
,
Gözde K. Erdoğan
Öz
In this study, it was aimed to determine the total antioxidant capacity and free radical scavenging activity of the extracts obtained by ultrasonic assisted extraction of oat grains, as well as to determine the fatty acid profile and total tocopherol content of oat extracts. For this purpose, we determined the total antioxidant capacity of oat grains extracts according to the copper ions (Cu2+) reducing antioxidant capacity (CUPRAC) test. At the same time, the radical scavenging capacity of oat extracts was investigated by the 1,1-diphenyl-2-picryl-hydrazil (DPPH•) method. Fatty acid composition of oat extract was determined via GC-FID analysis. Total tocopherol content was determined spectrophotometrically. The total antioxidant capacity of the oat grain extract was calculated as 1.5 ± 0.03 mmol TR 100g-1-oat grains extract by CUPRAC method. The DPPH• radical scavenging activity of the oat extract was calculated as 2.02 ± 0.01 mmol TR 100 g-1 – oat extract. Oat extracts exhibited potent antioxidant and radical scavenging ability. GC-FID results revealed that oat extract was rich in unsaturated fatty acids (Oleic acid, 43%; Linoleic acid, 34%). Total tocopherol content of oat oil extracts was 1376.3 ± 16 mg kg-1-oat extract.
Kaynakça
- Ak T, Gülçin İ 2008. Antioxidant and Radical Scavenging Properties of Curcumin. Chemico-Biological Interactions, 174(1): 27-37.
- Apak R, Güçlü K, Özyürek M, Esin Karademir S, Erçağ E 2006. The Cupric Ion Reducing Antioxidant Capacity and Polyphenolic Content of Some Herbal Teas. International Journal of Food Sciences and Nutrition, 57(5-6): 292-304.
- Apak R, Güçlü K, Özyürek M, Karademir SE 2004. Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capability in The Presence of Neocuproine: CUPRAC Method, Journal of Agricultural and Food Chemistry, 52:7970-7981
- Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA 1990. Apparent Hydroxyl Radical Production by Peroxynitrite: Implications for Endothelial Injury from Nitric Oxide and Superoxide. Proceedings of The National Academy of Sciences, 87(4): 1620-1624.
- Bener M, Şen FB, Önem AN, Bekdeşer B, Çelik SE, Lalikoglu M, Apak R 2022. Microwave-Assisted Extraction of Antioxidant Compounds from by-Products of Turkish Hazelnut (Corylus avellana L.) Using Natural Deep Eutectic Solvents: Modeling, Optimization and Phenolic Characterization. Food Chemistry, 385:132633.
- Biel W, Jacyno E, Kawęcka M 2014. Chemical Composition of Hulled, Dehulled and Naked Oat Grains. South African Journal of Animal Science, 44(2): 189-197.
- Boukhenouna S, Wilson MA, Bahmed K, Kosmider B 2018. Reactive Oxygen Species in Chronic Obstructive Pulmonary Disease. Oxidative Medicine and Cellular Longevity, 2018.
- Bryngelsson S, Mannerstedt‐Fogelfors B, Kamal‐Eldin A, Andersson R, Dimberg LH 2002. Lipids and Antioxidants in Groats and Hulls of Swedish Oats (Avena sativa L). Journal of the Science of Food and Agriculture, 82(6): 606-614.
- Çakmakçi S, Kahyaoğlu, DT 2012. Yağ Asitlerinin Sağlık ve Beslenme Üzerine Etkileri. Türk Bilimsel Derlemeler Dergisi, 2:133-137.
- Carlson MO, Montilla-Bascon G, Hoekenga OA, Tinker NA, Poland J, Baseggio M, Yeats TH 2019. Multivariate Genome-Wide Association Analyses Reveal The Genetic Basis of Seed Fatty Acid Composition in Oat (Avena sativa L.). G3: Genes, Genomes, Genetics, 9(9): 2963-2975.
- Carlsson-Kanyama A, González AD 2009. Potential Contributions of Food Consumption Patterns to Climate Change. American Journal of Clinical Nutrition, 89(5): 1704–1709.
- Carreira-Casais A, Otero P, Garcia-Perez P, Garcia-Oliveira P, Pereira AG, Carpena M, Prieto MA 2021. Benefits and Drawbacks of Ultrasound-Assisted Extraction for The Recovery of Bioactive Compounds from Marine Algae. International Journal of Environmental Research and Public Health, 18(17): 9153.
- Çelik SE, Özyürek M, Altun M, Bektaşoğlu B, Güçlü K, Berker KI, Apak R 2008. Antioxidant Capacities of Herbal Plants Used in The Manufacture of Van Herby Cheese:‘Otlu Peynir’. International Journal of Food Properties, 11(4):747-761.
- Çelik SE, Özyürek M, Güçlü K, Apak R 2010. Solvent Effects on The Antioxidant Capacity of Lipophilic and Hydrophilic Antioxidants Measured by CUPRAC, ABTS/Persulphate and FRAP Methods. Talanta, 81(4-5): 1300-1309.
- Chen H, Qiu S, Gan J, Li Z, Nirasawa S, Yin L. 2016. New Insights into The Antioxidant Activity and Components in Crude Oat Oil and Soybean Oil. Journal of Food Science and Technology, 53(1): 808-815.
Dedebaş T, Capar TD, Ekici L, Yalçin H 2021. Yağlı Tohumlarda Ultrasonik-Destekli Ekstraksiyon Yöntemi ve Avantajları. Avrupa Bilim ve Teknoloji Dergisi, 21: 313-322.
- Erdoğan Ü, Gökçe EH 2021. Fig Seed Oil‐Loaded Nanostructured Lipid Carriers: Evaluation of The Protective Effects Against Oxidation. Journal of Food Processing and Preservation, 45(10): e15835.
- Fathi‐Achachlouei B, Azadmard‐Damirchi S 2009. Milk Thistle Seed Oil Constituents from Different Varieties Grown in Iran. Journal of The American Oil Chemists' Society, 86(7): 643-649.
- Fritsche S, Wang X, Jung C 2017. Recent Advances in Our Understanding of Tocopherol Biosynthesis In Plants: An Overview of Key Genes, Functions, and Breeding of Vitamin E Improved Crops. Antioxidants, 6(4): 99.
- Godic A, Poljšak B, Adamic M, Dahmane R 2014. The Role of Antioxidants In Skin Cancer Prevention and Treatment. Oxidative Medicine and Cellular Longevity, 2014.
- Gogus U, Smith C 2010. n‐3 Omega fatty acids: A Review of Current Knowledge. International Journal of Food Science & Technology, 45(3): 417-436.
- Graf J 2005. Anti-Aging Skin Care Ingredient Technologies. Cheryl M. Burgess (Ed.), Cosmetic Dermatology İçinde (s.17-26). Berlin: Springer-Verlag GmbH.
- Gulzar S, Benjakul S. 2018. Ultrasound Waves Increase The Yield and Carotenoid Content of Lipid Extracted from Cephalothorax of Pacific White Shrimp (Litopenaeus vannamei). European Journal of Lipid Science and Technology, 120(5): 1700495.
- Keynes RG, Garthwaite J 2004. Nitric Oxide and Its Role in Ischaemic Brain injury. Current Molecular Medicine, 4(2):179-191.
- Kilci A, Gocmen D 2014. Phenolic Acid Composition, Antioxidant Activity and Phenolic Content of Tarhana Supplemented with Oat Flour. Food Chemistry, 151: 547–553.
- Kouřimská L, Sabolová M, Horčička P, Rys S, Božik M 2018. Lipid Content, Fatty Acid Profile, and Nutritional Value of New Oat Cultivars. Journal of Cereal Science, 84: 44-48.
- Kumar BS, Rathod BS, Gupta AK, Ravinder J 2017 Effect of Nitrogen Levels and Cutting Management on Available Nitrogen, Phosphorus and Potassium of Soil in Fodder Oat (Avena sativa L.) Cultivation. International Journal of Chemical Studies, 5: 146–148
- Li H, Pordesimo L, Weiss J 2004. High Intensity Ultrasound-Assisted Extraction of Oil from Soybeans. Food Research International, 37(7): 731-738
- Liu J, Jin S, Song H, Huang K, Li S, Guan X, Wang Y 2020. Effect of Extrusion Pretreatment on Extraction, Quality and Antioxidant Capacity of Oat (Avena sativa L.) Bran Oil. Journal of Cereal Science, 95: 102972.
- Martínez-Villaluenga C, Peñas E 2017. Health Benefits of Oat: Current Evidence and Molecular Mechanisms. Current Opinion in Food Science, 14: 26–31.
- McCusker MM, Grant-Kels JM 2010. Healing Fats of The Skin: The Structural and Immunologic Roles of The ω-6 and ω-3 Fatty Acids. Clinics in Dermatology, 28(4): 440-451.
- Meguro S, Arai Y, Masukawa Y, Uie K, Tokimitsu I 2000. Relationship between Covalently Bound Ceramides and Transepidermal Water Loss (TEWL). Archives of Dermatological Research, 292(9): 463-468.
- Musa Özcan M, Özkan G, Topal A 2006. Characteristics of Grains and Oils of Four Different Oats (Avena sativa L.) Cultivars Growing in Turkey. International Journal of Food Sciences and Nutrition, 57(5-6): 345-352.
- Newell MA, Asoro FG, Scott MP, White PJ, Beavis WD, Jannink JL 2012. Genome-Wide Association Study for Oat (Avena sativa L.) Beta-Glucan Concentration Using Germplasm of Worldwide Origin. Theoretical and Applied Genetics, 125(8): 1687-1696.
- Okur İ, Baltacıoğlu C, Ağçam E, Baltacıoğlu H, Alpas H 2019. Evaluation of The Effect of Different Extraction Techniques on Sour Cherry Pomace Phenolic Content and Antioxidant Activity and Determination of Phenolic Compounds by FTIR and HPLC. Waste and Biomass Valorization, 10(12): 3545-3555.
- Önder S, Tonguç M, Erbaş S, Önder D, Mutlucan M 2022. Investigation of Phenological, Primary and Secondary Metabolites Changes during Flower Developmental of Rosa damascena. Plant Physiology and Biochemistry, 192: 20-34.
- Peterson DM 2001. Oat Antioxidants, Journal of Cereal Science, 33(2): 115–129.
- Raes K, De Smet S, Demeyer D 2004. Effect of Dietary Fatty Acids on Incorporation of Long Chain Polyunsaturated Fatty Acids and Conjugated Linoleic Acid in Lamb, Beef and Pork Meat: A Review. Animal feed science and technology, 113(1-4): 199-221.
- Saleem M, Zamir MSI, Haq I, Irshad MZ, Rehman S 2015. Yield and Quality of Forage Oat (Avena sativa L.) Cultivars as Affected by Seed Inoculation with Nitrogenous Strains. American Journal of Plant Sciences, 6: 3251–3259
- Samaram S, Mirhosseini H, Tan CP, Ghazali HM, Bordbar S, Serjouie A 2015. Optimisation of Ultrasound-Assisted Extraction of Oil from Papaya Seed by Response Surface Methodology: Oil Recovery, Radical Scavenging Antioxidant Activity, and Oxidation stability. Food Chemistry, 172: 7-17.
- Singh R, De S, Belkheir A 2013. Avena Sativa (Oat), A Potential Neutraceutical and Therapeutic Agent: An Overview. Critical Reviews in Food Science and Nutrition, 53(2): 126-144.
- Stewart D, McDougall G 2014. Oat Agriculture, Cultivation and Breeding Targets: Implications for Human Nutrition and Health. British Journal of Nutrition, 112(S2): S50-S57.
- Toma M, Vinatoru M, Paniwnyk L, Mason TJ 2001. Investigation of The Effects of Ultrasound on Vegetal Tissues During Solvent Extraction. Ultrasonics sonochemistry, 8(2): 137-142.
- Vermaak I, Kamatou GPP, Komane-Mofokeng B, Viljoen AM, Beckett K 2011. African Seed Oils of Commercial Importance—Cosmetic Applications. South African Journal of Botany, 77(4): 920-933.
- Villaño D, Fernández-Pachón MS, Moyá ML, Troncoso AM, García-Parrilla MC 2007. Radical Scavenging Ability of Polyphenolic Compounds Towards DPPH Free Radical. Talanta, 71(1): 230-235.
- Vinatoru M, Toma M, Radu O, Filip PI, Lazurca D, Mason TJ. 1997. The Use of Ultrasound for The Extraction of Bioactive Principles from Plant Materials. Ultrasonics Sonochemistry, 4(2): 135-139.
- Wong ML, Timms RE, Goh E M 1988. Colorimetric Determination of Total Tocopherols in Palm Oil, Olein and Stearin. Journal of The American Oil Chemists Society, 65(2): 258-261.
- Yang Z, Piironen V, Lampi AM 2019. Epoxy and Hydroxy Fatty Acids As Non-Volatile Lipid Oxidation Products in Oat. Food chemistry, 295: 82-93.
- Zakirullah M, Ali N, Jan T, Khil A, Hazratullah IM 2017. Effect of Different Nitrogen Levels and Cutting Stages on Crude Protein, Crude Fiber, Dry Matter and Green Fodder Yield of Oat (Avena sativa L.). Pure and Applied Biology (PAB), 6(2): 448-453.
- Zhang QA, Zhang ZQ, Yue XF, Fan XH, Li T, Chen SF 2009. Response Surface Optimization of Ultrasound-Assisted Oil Extraction from Autoclaved Almond Powder. Food Chemistry, 116(2): 513-518.
- Zou Y, Lu Y, Wei D 2004. Antioxidant Activity of A Flavonoid-Rich Extract of Hypericum Perforatum L. In Vitro. Journal of Agricultural and Food Chemistry, 52(16): 5032-5039.
Yulaf (Avena sativa L.) Tanelerinin Ultrasonik Destekli Etanolik Ekstraksiyonunun Toplam Antioksidan Kapasitesi, Radikal Süpürücü Aktivitesi ve Yağ Asidi Kompozisyonunun Belirlenmesi
Yıl 2022,
, 326 - 334, 30.12.2022
Ümit Erdoğan
,
Gözde K. Erdoğan
Öz
Bu çalışmada yulaf tanelerinin ultrasonik destekli ekstraksiyonu ile elde edilen ekstraktların toplam antioksidan kapasitesi, serbest radikal süpürme aktivetisinin belirlenmesinin yanı sıra yulaf ekstraktının yağ asidi profili ve toplam tokoferol içeriğinin belirlenmesi amaçlandı. Bu amaçla, bakır iyonları (Cu2+) azaltıcı antioksidan güç testi (CUPRAC) metoduna göre yulaf taneleri ekstraktlarının toplam antioksidan kapasitesi belirlendi. Aynı zamanda, 1,1-difenil-2-pikril-hidrazil (DPPH•) yöntemine göre yulaf ekstraktlarının radikal süpürme kapasitesi araştırıldı. Yulaf ekstraktının yağ asidi komposizyonu GC-FID analizlerine göre belirlendi. Toplam tokoferol içeriği ise spektrofotometrik olarak belirlendi. Yulaf taneleri ekstraktının CUPRAC metoduna göre toplam antioksidan kapasitesi 1.5 ± 0.03 mmol TR /100g-yulaf taneleri ekstraktı olarak hesaplandı. Yulaf ekstraktının DPPH• radikali süpürme aktivitesi ise 2.02 ± 0.01 mmol TR /100 g – yulaf ekstraktı olarak hesaplandı. Yulaf özleri, güçlü antioksidan ve radikal süpürme yeteneği sergiledi. GC-FID sonuçları yulaf ekstraktının doymamış yağ asitlerince zengin olduğunu (Oleik asit, %43; Linoleik asit, %34) ortaya çıkardı. Yulaf taneleri ekstraktlarının toplam tokoferol içeriği ise 1376.3 ± 16 mg/kg-yulaf ekstrakt olarak bulundu.
Kaynakça
- Ak T, Gülçin İ 2008. Antioxidant and Radical Scavenging Properties of Curcumin. Chemico-Biological Interactions, 174(1): 27-37.
- Apak R, Güçlü K, Özyürek M, Esin Karademir S, Erçağ E 2006. The Cupric Ion Reducing Antioxidant Capacity and Polyphenolic Content of Some Herbal Teas. International Journal of Food Sciences and Nutrition, 57(5-6): 292-304.
- Apak R, Güçlü K, Özyürek M, Karademir SE 2004. Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capability in The Presence of Neocuproine: CUPRAC Method, Journal of Agricultural and Food Chemistry, 52:7970-7981
- Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA 1990. Apparent Hydroxyl Radical Production by Peroxynitrite: Implications for Endothelial Injury from Nitric Oxide and Superoxide. Proceedings of The National Academy of Sciences, 87(4): 1620-1624.
- Bener M, Şen FB, Önem AN, Bekdeşer B, Çelik SE, Lalikoglu M, Apak R 2022. Microwave-Assisted Extraction of Antioxidant Compounds from by-Products of Turkish Hazelnut (Corylus avellana L.) Using Natural Deep Eutectic Solvents: Modeling, Optimization and Phenolic Characterization. Food Chemistry, 385:132633.
- Biel W, Jacyno E, Kawęcka M 2014. Chemical Composition of Hulled, Dehulled and Naked Oat Grains. South African Journal of Animal Science, 44(2): 189-197.
- Boukhenouna S, Wilson MA, Bahmed K, Kosmider B 2018. Reactive Oxygen Species in Chronic Obstructive Pulmonary Disease. Oxidative Medicine and Cellular Longevity, 2018.
- Bryngelsson S, Mannerstedt‐Fogelfors B, Kamal‐Eldin A, Andersson R, Dimberg LH 2002. Lipids and Antioxidants in Groats and Hulls of Swedish Oats (Avena sativa L). Journal of the Science of Food and Agriculture, 82(6): 606-614.
- Çakmakçi S, Kahyaoğlu, DT 2012. Yağ Asitlerinin Sağlık ve Beslenme Üzerine Etkileri. Türk Bilimsel Derlemeler Dergisi, 2:133-137.
- Carlson MO, Montilla-Bascon G, Hoekenga OA, Tinker NA, Poland J, Baseggio M, Yeats TH 2019. Multivariate Genome-Wide Association Analyses Reveal The Genetic Basis of Seed Fatty Acid Composition in Oat (Avena sativa L.). G3: Genes, Genomes, Genetics, 9(9): 2963-2975.
- Carlsson-Kanyama A, González AD 2009. Potential Contributions of Food Consumption Patterns to Climate Change. American Journal of Clinical Nutrition, 89(5): 1704–1709.
- Carreira-Casais A, Otero P, Garcia-Perez P, Garcia-Oliveira P, Pereira AG, Carpena M, Prieto MA 2021. Benefits and Drawbacks of Ultrasound-Assisted Extraction for The Recovery of Bioactive Compounds from Marine Algae. International Journal of Environmental Research and Public Health, 18(17): 9153.
- Çelik SE, Özyürek M, Altun M, Bektaşoğlu B, Güçlü K, Berker KI, Apak R 2008. Antioxidant Capacities of Herbal Plants Used in The Manufacture of Van Herby Cheese:‘Otlu Peynir’. International Journal of Food Properties, 11(4):747-761.
- Çelik SE, Özyürek M, Güçlü K, Apak R 2010. Solvent Effects on The Antioxidant Capacity of Lipophilic and Hydrophilic Antioxidants Measured by CUPRAC, ABTS/Persulphate and FRAP Methods. Talanta, 81(4-5): 1300-1309.
- Chen H, Qiu S, Gan J, Li Z, Nirasawa S, Yin L. 2016. New Insights into The Antioxidant Activity and Components in Crude Oat Oil and Soybean Oil. Journal of Food Science and Technology, 53(1): 808-815.
Dedebaş T, Capar TD, Ekici L, Yalçin H 2021. Yağlı Tohumlarda Ultrasonik-Destekli Ekstraksiyon Yöntemi ve Avantajları. Avrupa Bilim ve Teknoloji Dergisi, 21: 313-322.
- Erdoğan Ü, Gökçe EH 2021. Fig Seed Oil‐Loaded Nanostructured Lipid Carriers: Evaluation of The Protective Effects Against Oxidation. Journal of Food Processing and Preservation, 45(10): e15835.
- Fathi‐Achachlouei B, Azadmard‐Damirchi S 2009. Milk Thistle Seed Oil Constituents from Different Varieties Grown in Iran. Journal of The American Oil Chemists' Society, 86(7): 643-649.
- Fritsche S, Wang X, Jung C 2017. Recent Advances in Our Understanding of Tocopherol Biosynthesis In Plants: An Overview of Key Genes, Functions, and Breeding of Vitamin E Improved Crops. Antioxidants, 6(4): 99.
- Godic A, Poljšak B, Adamic M, Dahmane R 2014. The Role of Antioxidants In Skin Cancer Prevention and Treatment. Oxidative Medicine and Cellular Longevity, 2014.
- Gogus U, Smith C 2010. n‐3 Omega fatty acids: A Review of Current Knowledge. International Journal of Food Science & Technology, 45(3): 417-436.
- Graf J 2005. Anti-Aging Skin Care Ingredient Technologies. Cheryl M. Burgess (Ed.), Cosmetic Dermatology İçinde (s.17-26). Berlin: Springer-Verlag GmbH.
- Gulzar S, Benjakul S. 2018. Ultrasound Waves Increase The Yield and Carotenoid Content of Lipid Extracted from Cephalothorax of Pacific White Shrimp (Litopenaeus vannamei). European Journal of Lipid Science and Technology, 120(5): 1700495.
- Keynes RG, Garthwaite J 2004. Nitric Oxide and Its Role in Ischaemic Brain injury. Current Molecular Medicine, 4(2):179-191.
- Kilci A, Gocmen D 2014. Phenolic Acid Composition, Antioxidant Activity and Phenolic Content of Tarhana Supplemented with Oat Flour. Food Chemistry, 151: 547–553.
- Kouřimská L, Sabolová M, Horčička P, Rys S, Božik M 2018. Lipid Content, Fatty Acid Profile, and Nutritional Value of New Oat Cultivars. Journal of Cereal Science, 84: 44-48.
- Kumar BS, Rathod BS, Gupta AK, Ravinder J 2017 Effect of Nitrogen Levels and Cutting Management on Available Nitrogen, Phosphorus and Potassium of Soil in Fodder Oat (Avena sativa L.) Cultivation. International Journal of Chemical Studies, 5: 146–148
- Li H, Pordesimo L, Weiss J 2004. High Intensity Ultrasound-Assisted Extraction of Oil from Soybeans. Food Research International, 37(7): 731-738
- Liu J, Jin S, Song H, Huang K, Li S, Guan X, Wang Y 2020. Effect of Extrusion Pretreatment on Extraction, Quality and Antioxidant Capacity of Oat (Avena sativa L.) Bran Oil. Journal of Cereal Science, 95: 102972.
- Martínez-Villaluenga C, Peñas E 2017. Health Benefits of Oat: Current Evidence and Molecular Mechanisms. Current Opinion in Food Science, 14: 26–31.
- McCusker MM, Grant-Kels JM 2010. Healing Fats of The Skin: The Structural and Immunologic Roles of The ω-6 and ω-3 Fatty Acids. Clinics in Dermatology, 28(4): 440-451.
- Meguro S, Arai Y, Masukawa Y, Uie K, Tokimitsu I 2000. Relationship between Covalently Bound Ceramides and Transepidermal Water Loss (TEWL). Archives of Dermatological Research, 292(9): 463-468.
- Musa Özcan M, Özkan G, Topal A 2006. Characteristics of Grains and Oils of Four Different Oats (Avena sativa L.) Cultivars Growing in Turkey. International Journal of Food Sciences and Nutrition, 57(5-6): 345-352.
- Newell MA, Asoro FG, Scott MP, White PJ, Beavis WD, Jannink JL 2012. Genome-Wide Association Study for Oat (Avena sativa L.) Beta-Glucan Concentration Using Germplasm of Worldwide Origin. Theoretical and Applied Genetics, 125(8): 1687-1696.
- Okur İ, Baltacıoğlu C, Ağçam E, Baltacıoğlu H, Alpas H 2019. Evaluation of The Effect of Different Extraction Techniques on Sour Cherry Pomace Phenolic Content and Antioxidant Activity and Determination of Phenolic Compounds by FTIR and HPLC. Waste and Biomass Valorization, 10(12): 3545-3555.
- Önder S, Tonguç M, Erbaş S, Önder D, Mutlucan M 2022. Investigation of Phenological, Primary and Secondary Metabolites Changes during Flower Developmental of Rosa damascena. Plant Physiology and Biochemistry, 192: 20-34.
- Peterson DM 2001. Oat Antioxidants, Journal of Cereal Science, 33(2): 115–129.
- Raes K, De Smet S, Demeyer D 2004. Effect of Dietary Fatty Acids on Incorporation of Long Chain Polyunsaturated Fatty Acids and Conjugated Linoleic Acid in Lamb, Beef and Pork Meat: A Review. Animal feed science and technology, 113(1-4): 199-221.
- Saleem M, Zamir MSI, Haq I, Irshad MZ, Rehman S 2015. Yield and Quality of Forage Oat (Avena sativa L.) Cultivars as Affected by Seed Inoculation with Nitrogenous Strains. American Journal of Plant Sciences, 6: 3251–3259
- Samaram S, Mirhosseini H, Tan CP, Ghazali HM, Bordbar S, Serjouie A 2015. Optimisation of Ultrasound-Assisted Extraction of Oil from Papaya Seed by Response Surface Methodology: Oil Recovery, Radical Scavenging Antioxidant Activity, and Oxidation stability. Food Chemistry, 172: 7-17.
- Singh R, De S, Belkheir A 2013. Avena Sativa (Oat), A Potential Neutraceutical and Therapeutic Agent: An Overview. Critical Reviews in Food Science and Nutrition, 53(2): 126-144.
- Stewart D, McDougall G 2014. Oat Agriculture, Cultivation and Breeding Targets: Implications for Human Nutrition and Health. British Journal of Nutrition, 112(S2): S50-S57.
- Toma M, Vinatoru M, Paniwnyk L, Mason TJ 2001. Investigation of The Effects of Ultrasound on Vegetal Tissues During Solvent Extraction. Ultrasonics sonochemistry, 8(2): 137-142.
- Vermaak I, Kamatou GPP, Komane-Mofokeng B, Viljoen AM, Beckett K 2011. African Seed Oils of Commercial Importance—Cosmetic Applications. South African Journal of Botany, 77(4): 920-933.
- Villaño D, Fernández-Pachón MS, Moyá ML, Troncoso AM, García-Parrilla MC 2007. Radical Scavenging Ability of Polyphenolic Compounds Towards DPPH Free Radical. Talanta, 71(1): 230-235.
- Vinatoru M, Toma M, Radu O, Filip PI, Lazurca D, Mason TJ. 1997. The Use of Ultrasound for The Extraction of Bioactive Principles from Plant Materials. Ultrasonics Sonochemistry, 4(2): 135-139.
- Wong ML, Timms RE, Goh E M 1988. Colorimetric Determination of Total Tocopherols in Palm Oil, Olein and Stearin. Journal of The American Oil Chemists Society, 65(2): 258-261.
- Yang Z, Piironen V, Lampi AM 2019. Epoxy and Hydroxy Fatty Acids As Non-Volatile Lipid Oxidation Products in Oat. Food chemistry, 295: 82-93.
- Zakirullah M, Ali N, Jan T, Khil A, Hazratullah IM 2017. Effect of Different Nitrogen Levels and Cutting Stages on Crude Protein, Crude Fiber, Dry Matter and Green Fodder Yield of Oat (Avena sativa L.). Pure and Applied Biology (PAB), 6(2): 448-453.
- Zhang QA, Zhang ZQ, Yue XF, Fan XH, Li T, Chen SF 2009. Response Surface Optimization of Ultrasound-Assisted Oil Extraction from Autoclaved Almond Powder. Food Chemistry, 116(2): 513-518.
- Zou Y, Lu Y, Wei D 2004. Antioxidant Activity of A Flavonoid-Rich Extract of Hypericum Perforatum L. In Vitro. Journal of Agricultural and Food Chemistry, 52(16): 5032-5039.