Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro.

Semih Dalkılıç; Lütfiye Kadıoğlu Dalkılıç; Songül Fidan; Mustafa Timurkaan; Lütfü Uygur; Gökhan Akay; İsmail Korkmaz

doi:10.18016/ksutarimdoga.vi.1192081

Araştırma Makalesi

Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro.

Yıl 2023, , 1291 - 1304, 31.12.2023

Semih Dalkılıç , Lütfiye Kadıoğlu Dalkılıç , Songül Fidan , Mustafa Timurkaan , Lütfü Uygur , Gökhan Akay , İsmail Korkmaz

https://doi.org/10.18016/ksutarimdoga.vi.1192081

Cited By: 2

Öz

Mentha pulegium is a medicinally important and well-known plant and used for various purposes such as medicinal, nutritional and spice. We are analyzed to observe the antimicrobial, antioxidant and cytotoxic properties of M. pulegium extracts in this study. The antimicrobial activity of M. pulegium was tested using the agar well method. MIC, MBC and antimicrobial activity were tested on Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25322), Bacillus megaterium (DSM32) and Candida albicans (FMC17) microorganisms. Clindamycin (2 µg) and Amoxicillin (30 µg) were used as positive control and Dimethylsulfoxide (DMSO) as negative control. Cytotoxic activity of extracts at different concentrations obtained from solvents such as acetone, chloroform and methanol using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test method; µg/ml cytotoxic activity and cell death patterns after exposure to different concentrations of the extracts on human breast cancer (MDA-MB-231), human pancreatic cancer (PANC1), human ovarian cancer (OVCAR3) and human lung cancer (A549) cell lines determined accordingly. As a result, it was determined that M. pulegium extract has a strong antimicrobial activity spectrum and cytotoxic effect.

Anahtar Kelimeler

Antimicrobial, Antioxidant, Apoptosis, Cytotoxicity, Mentha pulegium

Destekleyen Kurum

TÜBİTAK

Proje Numarası

1919B012001186

Teşekkür

Thanks to Prof. Dr. Mustafa Kaplan, Fırat University Faculty of Medicine Department of Parasitology, for contributing to the laboratory environment in the realization of this study.

Kaynakça

1. Awouafack MD, McGaw LJ, Gottfried S, Mbouangouere R, Tane P, Spiteller M, et al. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae). BMC Complementary and Alternative Medicine. 2013;13(1):1-9. https://doi.org/10.1186/1472-6882-13-289
2. Ahmed A, Ayoub K, Chaima AJ, Hanaa L, Abdelaziz C. Effect of drying methods on yield, chemical composition and bioactivities of essential oil obtained from Moroccan Mentha pulegium L. Biocatalysis and Agricultural Biotechnology. 2018;16:638-643. https://doi.org/10.1016/j.bcab.2018.10.016
3. Andersson DI, Hughes D. Persistence of antibiotic resistance in bacterial populations. FEMS Microbiology Reviews. 2011;35(5):901-911. https://doi.org/10.1111/j.1574-6976.2011.00289.x
4. Benlarbi KH, Elmtili N, Macías FA, Galindo JCG. Influence of in vitro growth conditions in the production of defence compounds in Mentha pulegium L. Phytochemistry Letters. 2014;8:233-244. https://doi.org/10.1016/j.phytol.2014.03.007
5. Buys SS, Partridge E, Greene MH, Prorok PC, Reding D, Riley TL, Hartge P, Fagerstrom RM, Ragard LR, Chia D, Izmirlian G, Fouad M, Johnson CC, Gohagan JK, PLCO Project Team. Ovarian cancer screening in the Prostate, Lung, Colorectal and Ovarian (PLCO) cancer screening trial: findings from the initial screen of a randomized trial. American Journal of Obstetrics and Gynecology. 2005;193(5):1630–1639. https://doi.org/10.1016/j.ajog.2005.05.005
6. Chalchat JC, Gorunovic MS, Maksimovic ZA, Petrovic SD. Essential oil of wild growing Mentha Pulegium L. from Yugoslavia. Journal of Essential Oil Research. 2000;12(5):598-600. https://doi.org/10.1080/10412905.2000.9712166
7. Cevatemre B. Fenretinid ve indol-3-karbinol kombinasyonunun meme kanseri hücre soyları üzerindeki sitotoksik/apoptotik etkilerinin araştırılması [Master's thesis]. Bursa: Uludağ Üniversitesi; 2012. Türkçe.
8. Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 1999;12(4):564–582. https://doi.org/10.1128/CMR.12.4.564
9. Coşkun MD. Nf-kb ve oksidatif stresin inhibisyonu ile peripheral nöropatinin iyileştirilmesi ve kanser kemoterapi sonuçlarının geliştirilmesi [Doctoral thesis]. Bursa: Uludağ Üniversitesi; 2017.Türkçe.
10. Caceres A, Cano O, Samayoa B, Aguilar L. Plants used in guatemala for the treatment of gastrointestinal disorders. 1. Screening of 84 plants against enterobacteria. Journal of Ethnopharmacology. 1990;30(1):55–73. https://doi.org/10.1016/0378-8741(90)90017-n
11. Çöteli E, Erden Y, Karataş F. Yarpuz (Mentha Pulegium L.) bitkisindeki malondialdehit, glutatyon ve vitamin miktarları ile total antioksidan kapasitesinin araştırılması. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2013;17(2):4-10. Türkçe. https://dergipark.org.tr/en/pub/sdufenbed/issue/20801/222003
12. Dorman HJD, Koşar M, Kahlos K, Holm Y, Hiltunen R. Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. Journal of Agricultural and Food Chemistry. 2003;51(16):4563–4569. https://doi.org/10.1021/jf034108k
13. Dalkılıç S, Dalkılıç LK, Korkmaz İ. Geleneksel kahvaltılık zahterin antimikrobiyal etkisi. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020;10(1):128-133. Türkçe. https://doi.org/10.17714/gumusfenbil.579489 14. Duru ME, Oztürk M, Uğur A, Ceylan O. The constituents of essential oil and in vitro antimicrobial activity of Micromeria cilicica from Turkey. Journal of Ethnopharmacology. 2000;94(1):43–48. https://doi.org/10.1016/j.jep.2004.03.053
15. Dutta D, Chakraborty A, Mukherjee B, Gupta S. Aptamer-conjugated apigenin nanoparticles to target colorectal carcinoma: a promising safe alternative of colorectal cancer chemotherapy. ACS Applied Bio Materials. 2018;1(5):1538-1556. https://doi.org/10.1021/acsabm.8b00441
16. Erarslan ZB, Ecevit Genç G, Kültür Ş. Medicinal plants traditionally used to treat skin diseases in Turkey–eczema, psoriasis, vitiligo. Journal of Faculty of Pharmacy of Ankara University. 2020;44(1):137-166. https://doi.org/10.33483/jfpau.586114
17. Erdogan EA, Everest A, Kaplan E. Antimicrobial activities of aqueous extracts and essential oils of two endemic species from Turkey. Indian Journal of Traditional Knowledge. 2013;12(2):221-4.
18. Ebana RUB, Etim OE, Edet UO, John RS, Osom AO. Comparative evaluation of phytochemicals and antibacterial activity of the roots and leaves of anthocleista vogelii on some clinical ısolates. Asian Journal of Research in Botany. 2018;1(1):1-8. https://journalajrib.com/index.php/AJRIB/article/view/1118
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21. González-Tejero MR, Casares-Porcel M, Sánchez-Rojas CP, Ramiro-Gutiérrez JM, Molero-Mesa J, Pieroni A, et al. Medicinal plants in the Mediterranean area: synthesis of the results of the project Rubia. Journal of Ethnopharmacology. 2008; 116(2):341–357. https://doi.org/10.1016/j.jep.2007.11.045
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Mentha pulegium ekstraktı güçlü in vitro antimikrobiyal ve sitotoksik etki göstermiştir

Yıl 2023, , 1291 - 1304, 31.12.2023

Semih Dalkılıç , Lütfiye Kadıoğlu Dalkılıç , Songül Fidan , Mustafa Timurkaan , Lütfü Uygur , Gökhan Akay , İsmail Korkmaz

https://doi.org/10.18016/ksutarimdoga.vi.1192081

Cited By: 2

Öz

Mentha pulegium tıbbi açıdan önemli ve iyi bilinen bir bitkidir ve tıbbi, beslenme ve baharat gibi çeşitli amaçlar için kullanılır. Bu çalışmada M. pulegium ekstraktının antimikrobiyal, antioksidan ve sitotoksik özelliklerini görmek için analiz edildi. M. pulegium'un antimikrobiyal aktivitesi agar kuyucuk yöntemi kullanılarak test edildi. MIC, MBC ve antimikrobiyal aktivite Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25322), Bacillus megaterium (DSM32) ve Candida albicans (FMC17) mikroorganizmaları üzerinde test edildi. Pozitif kontrol olarak Klindamisin (2 µg) ve Amoksisilin (30 µg), negatif kontrol olarak Dimetilsülfoksit (DMSO) kullanıldı. 3-(4,5-dimetiltiyazol-2-il) -2,5-difenil tetrazolyum bromür (MTT) test yöntemi kullanılarak aseton, kloroform ve metanol gibi çözücülerden elde edilen farklı konsantrasyonlarda ekstraktların sitotoksik aktivitesi; ekstraktların farklı konsantrasyonlarına maruz kaldıktan sonra µg/ml sitotoksik aktivite ve hücre ölüm şekilleri; Aseton, kloroform ve metanol gibi çözücülerden 3-(4,5-dimetiltiyazol-2-il) -2,5-difenil tetrazolyum bromür (MTT) test yöntemi kullanılarak aseton, kloroform ve metanol insan meme kanseri (MDA-MB-231), insan pankreas kanseri (PANC1), insan yumurtalık kanseri (OVCAR3) ve insan akciğer kanseri (A549) hücre hatları buna göre belirlendi. Sonuç olarak M. pulegium ekstraktının güçlü bir antimikrobiyal aktivite spektrumuna ve sitotoksik etkiye sahip olduğu belirlenmiştir.

Anahtar Kelimeler

Antimikrobiyal, Antioksidan, Apoptoz, Sitotoksik, Mentha pulegium

Proje Numarası

1919B012001186

Kaynakça

1. Awouafack MD, McGaw LJ, Gottfried S, Mbouangouere R, Tane P, Spiteller M, et al. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae). BMC Complementary and Alternative Medicine. 2013;13(1):1-9. https://doi.org/10.1186/1472-6882-13-289
2. Ahmed A, Ayoub K, Chaima AJ, Hanaa L, Abdelaziz C. Effect of drying methods on yield, chemical composition and bioactivities of essential oil obtained from Moroccan Mentha pulegium L. Biocatalysis and Agricultural Biotechnology. 2018;16:638-643. https://doi.org/10.1016/j.bcab.2018.10.016
3. Andersson DI, Hughes D. Persistence of antibiotic resistance in bacterial populations. FEMS Microbiology Reviews. 2011;35(5):901-911. https://doi.org/10.1111/j.1574-6976.2011.00289.x
4. Benlarbi KH, Elmtili N, Macías FA, Galindo JCG. Influence of in vitro growth conditions in the production of defence compounds in Mentha pulegium L. Phytochemistry Letters. 2014;8:233-244. https://doi.org/10.1016/j.phytol.2014.03.007
5. Buys SS, Partridge E, Greene MH, Prorok PC, Reding D, Riley TL, Hartge P, Fagerstrom RM, Ragard LR, Chia D, Izmirlian G, Fouad M, Johnson CC, Gohagan JK, PLCO Project Team. Ovarian cancer screening in the Prostate, Lung, Colorectal and Ovarian (PLCO) cancer screening trial: findings from the initial screen of a randomized trial. American Journal of Obstetrics and Gynecology. 2005;193(5):1630–1639. https://doi.org/10.1016/j.ajog.2005.05.005
6. Chalchat JC, Gorunovic MS, Maksimovic ZA, Petrovic SD. Essential oil of wild growing Mentha Pulegium L. from Yugoslavia. Journal of Essential Oil Research. 2000;12(5):598-600. https://doi.org/10.1080/10412905.2000.9712166
7. Cevatemre B. Fenretinid ve indol-3-karbinol kombinasyonunun meme kanseri hücre soyları üzerindeki sitotoksik/apoptotik etkilerinin araştırılması [Master's thesis]. Bursa: Uludağ Üniversitesi; 2012. Türkçe.
8. Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 1999;12(4):564–582. https://doi.org/10.1128/CMR.12.4.564
9. Coşkun MD. Nf-kb ve oksidatif stresin inhibisyonu ile peripheral nöropatinin iyileştirilmesi ve kanser kemoterapi sonuçlarının geliştirilmesi [Doctoral thesis]. Bursa: Uludağ Üniversitesi; 2017.Türkçe.
10. Caceres A, Cano O, Samayoa B, Aguilar L. Plants used in guatemala for the treatment of gastrointestinal disorders. 1. Screening of 84 plants against enterobacteria. Journal of Ethnopharmacology. 1990;30(1):55–73. https://doi.org/10.1016/0378-8741(90)90017-n
11. Çöteli E, Erden Y, Karataş F. Yarpuz (Mentha Pulegium L.) bitkisindeki malondialdehit, glutatyon ve vitamin miktarları ile total antioksidan kapasitesinin araştırılması. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2013;17(2):4-10. Türkçe. https://dergipark.org.tr/en/pub/sdufenbed/issue/20801/222003
12. Dorman HJD, Koşar M, Kahlos K, Holm Y, Hiltunen R. Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. Journal of Agricultural and Food Chemistry. 2003;51(16):4563–4569. https://doi.org/10.1021/jf034108k
13. Dalkılıç S, Dalkılıç LK, Korkmaz İ. Geleneksel kahvaltılık zahterin antimikrobiyal etkisi. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020;10(1):128-133. Türkçe. https://doi.org/10.17714/gumusfenbil.579489 14. Duru ME, Oztürk M, Uğur A, Ceylan O. The constituents of essential oil and in vitro antimicrobial activity of Micromeria cilicica from Turkey. Journal of Ethnopharmacology. 2000;94(1):43–48. https://doi.org/10.1016/j.jep.2004.03.053
15. Dutta D, Chakraborty A, Mukherjee B, Gupta S. Aptamer-conjugated apigenin nanoparticles to target colorectal carcinoma: a promising safe alternative of colorectal cancer chemotherapy. ACS Applied Bio Materials. 2018;1(5):1538-1556. https://doi.org/10.1021/acsabm.8b00441
16. Erarslan ZB, Ecevit Genç G, Kültür Ş. Medicinal plants traditionally used to treat skin diseases in Turkey–eczema, psoriasis, vitiligo. Journal of Faculty of Pharmacy of Ankara University. 2020;44(1):137-166. https://doi.org/10.33483/jfpau.586114
17. Erdogan EA, Everest A, Kaplan E. Antimicrobial activities of aqueous extracts and essential oils of two endemic species from Turkey. Indian Journal of Traditional Knowledge. 2013;12(2):221-4.
18. Ebana RUB, Etim OE, Edet UO, John RS, Osom AO. Comparative evaluation of phytochemicals and antibacterial activity of the roots and leaves of anthocleista vogelii on some clinical ısolates. Asian Journal of Research in Botany. 2018;1(1):1-8. https://journalajrib.com/index.php/AJRIB/article/view/1118
19. Faydaoğlu E, Sürücüoğlu M. Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidan aktiviteleri ve kullanım olanakları. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2013;6(2):233-265. Türkçe. https://dergipark.org.tr/en/pub/erzifbed/issue/6027/80707
20. Greenwell M, Rahman PKSM. Medicinal plants: their use in anticancer treatment. International Journal of Pharmaceutical Sciences And Research. 2015;6(10):4103. https://dx.doi.org/10.13040%2FIJPSR.0975-8232.6(10).4103-12
21. González-Tejero MR, Casares-Porcel M, Sánchez-Rojas CP, Ramiro-Gutiérrez JM, Molero-Mesa J, Pieroni A, et al. Medicinal plants in the Mediterranean area: synthesis of the results of the project Rubia. Journal of Ethnopharmacology. 2008; 116(2):341–357. https://doi.org/10.1016/j.jep.2007.11.045
22. Gülçin İ, Gören AC, Taslimi P, Alwasel SH, Kılıc O, Bursal E. Anticholinergic, antidiabetic and antioxidant activities of Anatolian pennyroyal (Mentha pulegium)-analysis of its polyphenol contents by LC-MS/MS. Biocatalysis and Agricultural Biotechnology. 2020;23:101441. https://doi.org/10.1016/j.bcab.2019.101441
23. Hassan E, Ullah H. Antibacterial and antifungal activities of the medicinal plant veronica biloba. Journal of Chemistry. 2019. https://doi.org/10.1155/2019/5264943
24. Hesham HM, Lasheen DS, Abouzid KA. Chimeric HDAC inhibitors: Comprehensive review on the HDAC-based strategies developed to combat cancer. Medicinal Research Reviews. 2018;38(6):2058-2109. https://doi.org/10.1002/med.21505
25. Hemeg HA, Moussa IM, Ibrahim S, Dawoud TM, Alhaji JH, Mubarak AS, et al. Antimicrobial effect of different herbal plant extracts against different microbial population. Saudi Journal of Biological Sciences. 2020; 27(12):3221–3227. https://doi.org/10.1016/j.sjbs.2020.08.015
26. . Kurt H, Keşkek ŞÖ, Çil T, Canataroğlu A. Meme kanserli hastalarda tamamlayıcı/alternatif tedavi kullanımı. Türk Onkoloji Dergisi. 2013; 28(1):10-15. Türkçe. https://doi.org/10.5505/tjoncol.2013.827
27. Lowy FD. Antimicrobial resistance: the example of Staphylococcus aureus. The Journal of Clinical Investigation. 2003;111(9):1265-1273. https://doi.org/10.1172/JCI18535
28. Liu Q, Meng X, Li Y, Zhao CN, Tang GY, Li HB. Antibacterial and antifungal activities of spices. International Journal of Molecular Sciences. 2017;18(6):1283. https://doi.org/10.3390/ijms18061283
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Toplam 50 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Yapısal Biyoloji
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Semih Dalkılıç 0000-0002-6892-247X Lütfiye Kadıoğlu Dalkılıç 0000-0002-6791-3811 Songül Fidan 0000-0003-1876-858X Mustafa Timurkaan 0000-0003-1950-0489 Lütfü Uygur 0000-0002-5113-2703 Gökhan Akay 0000-0003-3892-2572 İsmail Korkmaz 0000-0003-4631-7786
Proje Numarası	1919B012001186
Erken Görünüm Tarihi	14 Haziran 2023
Yayımlanma Tarihi	31 Aralık 2023
Gönderilme Tarihi	20 Ekim 2022
Kabul Tarihi	27 Şubat 2023
Yayımlandığı Sayı	Yıl 2023

Kaynak Göster

APA	Dalkılıç, S., Kadıoğlu Dalkılıç, L., Fidan, S., Timurkaan, M., vd. (2023). Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(6), 1291-1304. https://doi.org/10.18016/ksutarimdoga.vi.1192081