Altınözü İlçesinde Yetiştirilen Sarımsak (Allium sativum L.) Genotiplerinin Morfolojik ve Moleküler Karakterizasyonu

Faika Yaralı Karakan; Ayhan Kılıç; Berna Ergun Çetin

doi:10.18016/ksutarimdoga.vi.1324022

Araştırma Makalesi

Altınözü İlçesinde Yetiştirilen Sarımsak (Allium sativum L.) Genotiplerinin Morfolojik ve Moleküler Karakterizasyonu

Yıl 2024, , 333 - 343, 01.04.2024

Faika Yaralı Karakan , Ayhan Kılıç , Berna Ergun Çetin

https://doi.org/10.18016/ksutarimdoga.vi.1324022

Cited By: 2

Öz

Bu çalışma, Hatay ilinin Altınözü ilçesinde yetiştiriciliği yapılan sarımsak genotiplerinin morfolojik ve moleküler karakterizasyonunu yapmak amacıyla yürütülmüştür. Araştırma sonucunda genotipler arasında bitki boyu, yalancı gövde çapı, yalancı gövde uzunluğu, yaprak uzunluğu, yaprak genişliği, baş ağırlığı, baş uzunluğu, baş genişliği ve diş ağırlığı değerleri bakımından Mayadalı genotipi ön plana çıkarken, Çetenli genotipi ise diş sayısı bakımından diğer genotiplerden daha yüksek değere sahip olmuştur. Genotiplerin baş kabuklarında zemin rengi beyaz ve beyaz-sarımsı, başların dip şekli girintili ve yuvarlak, dişlerde et rengi sarımsı ve beyaz, diş dağılımı düzenli ve düzensiz, dişlerde kabuk rengi pembe, mor ve kahverengi olarak belirlenmiştir. UPGMA metoduna göre oluşturulan ve genotipler arası genetik yakınlığı gösteren dendograma göre sarımsak genotipleri iki ana gruba ayrılmıştır. Altınözü ilçesinde yetiştirilen sarımsak genotiplerinin özgünlüğünün ortaya konması bakımından önem taşıyan araştırmada tespit edilen genetik çeşitliliğin yeni sarımsak çeşitlerinin geliştirilmesine katkıda bulunacağı düşünülmektedir.

Anahtar Kelimeler

Allium sativum L., Sarımsak, Moleküler karakterizasyon, Morfolojik karakterizasyon, SSR

Teşekkür

Bu makale ikinci yazara ait yüksek lisans tezinden üretilmiştir.

Kaynakça

Akan, S. (2022). Morphological characterisation and volatile analysis of Turkish garlic genotypes. Turkish Journal of Agriculture and Forestry, 46(4), 424-440.
Akan, S., Horzum, Ö. & Tuna Güneş, N. (2019). Modifiye atmosferli paketlemenin yeşil sarımsak (Allium sativum L.) yapraklarında kalite korunumuna etkisi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 23(4),165-173. https://dergipark.org.tr/tr/download/article-file/890357
Ayed, C., Bayoudh, C., Rhimi, A., Mezghani, N., Haouala, F. & Dridi, B.A.M. (2018). In vitro propagation of tunisian local garlic (Allium Sativum L.) from shoot-tip culture. Journal of Horticulture and Postharvest Research, 1(2), 75-86. DOI: 10.22077/jhpr.2018.1457.1016. 10.22077/ JHPR.2018.1457.1016
Ayres, N.M., Mcclung, A.M., Larkin, P.D., Bligh, H.F.J., Jones, C.A. & Park, W.D. (1997). Microsatellite and a single nucleotide polymorphism differentiate apparent amylose classes in an extended pedigree of US rice germplasm. Theoretical and Applied Genetics, 94,773- 781. https://doi.org/10.1007/s001220050477
Barandiaran, X., Martin, N., Alba, C., Rodriguez-Conde, M.F., Pietro, A.D. & Martin, J. (1999). An efficient method for the ın vitro management of multiple garlic accessions. In Vitro Cell. Dev. Biol. Plant., 35, 466-469. https://doi.org/10.1007/s11627-999-0069-y
Beşirli, G., İnan, Y. & Türkeş, T. (1994). Sarımsak çeşit tespit denemesi, bilimsel araştırma ve incelemeler. Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü, Yayın No: 41, 14 s.,Yalova.
Buso, G.S.C., Paiva, M.R., Torres, A.C., Resende, F.V., Ferreira, M.A., Buso, J.A. & Dusi, A.N. (2008). Genetic diversity studies of Brazilian garlic cultivars and quality control of garlic-clover production. Genetics and Molecular Research, 7(2), 534-541. DOI: 10.4238/vol7-2gmr451
De-Oliveira, J.T., De-Oliveira, R.A., Cunha, F.F., Silva Ribeiro, I., Oliveira, L.A.A. & Teodoro, P.E. (2020). Contribution of morphological variables in garlic bulb yield. HortScience, 55(6), 896-897. https://doi.org/10.21273/HORTSCI14996-20
Doyle, J.J. & Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus,12: 13-15.
Etoh, T., & Simon, P.W. (2002). Diversity, fertility and seed production of garlic. in: Rabinowitch H.D. & Currah L. (eds) Allium Crop Science Recent Advances. CABI Publishing, Wallingford, UK, 101–117p.
Figliuolo, G., Candido, V., Logozzo, G., Miccolis, V. & Zeuli, P.S. (2001). Genetic evaluation of cultivated garlic germplasm Allium sativum L. and A. Ampeloprasum L. Euphytica. 121(3): 325-334. https://doi.org/10.1023/A:1012069532157
Garcia-Vallve, S., Palau J. & Romeu, A. (1999). Horizontal gene transfer in glycosyl hydrolases inferred from codon usage in Escherichia coli and Bacillus subtilis. Molecular Biology and Evololution, 16(9):1125-1134.
Geboloğlu, N., Karabekiroğlu, D.S., & Doksöz, S. (2017). Tokat sarımsağının morfolojik ve moleküler karekterizasyonu. Akademik Ziraat Dergisi, 6: 131-136. https://app.trdizin.gov.tr/makale/ TWpVMk 16SXlNZz09
Günaydın, Ö. (2011). Sarımsakta (Allium sativum L.) düşük sıcaklıkta depolama uygulamasından farklı etkilenen aday genlerin cDNA-AFLP yöntemi ile belirlenmesi (Tez no 284803). [Yüksek Lisans Tezi, Uludağ Üniversitesi, Fen Bilimleri Enstitüsü, Bahçe Bitkileri Ana Bilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
Jo, M.H., Ham, I.K., Moe, K.T., Kwon, S.W., Lu, F.H., Park, Y.J. & Lee, E.M. (2012). Classification of genetic variation in garlic (Allium sativum L.) using SSR markers. Australian Journal of Crop Science, 6(4), 625-631. https://search.informit.org/doi/ 10.3316/ informit.362158671579385
Khar, A., Asha Devi, A., Mahajan, V. & Lawande, K.E. (2006). Genetic divergence analysis in elite lines of garlic (Allium sativum L.). J. Maha. Agri. Univ., 31, 52- 55
Kıraç, H. (2019). Türkiye'de yaygın olarak yetiştiriciliği yapılan farklı sarımsak genotiplerinin morfolojik ve moleküler karakterizasyonu (Tez no: 560519). [Yüksek Lisans Tezi, Erciyes Üniversitesi Fen Bilimleri Enstitüsü Bahçe Bitkileri Anabilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
Kumar, M., Sharma, V. R., Kumar, V., Sirohi, U., Chaudhary, V., Sharma, S. & Sharma, S. (2019). Genetic diversity and population structure analysis of Indian garlic (Allium sativum L.) collection using SSR markers. Physiology and Molecular Biology of Plants, 25(2), 377-386. https://doi.org/10.1007/ s12298-018-0628-y
Manjunathagowda, D.C., Gopal, J. Archana, R. & Asiya, K.R. (2017). Virus–free seed production of garlic (Allium sativum L.): status and prospects. Int.J.Curr.Microbiol.App.Sci., 6(6), 2446-2456. https://doi.org/10.20546/ijcmas.2017.606.290
Paredes, M., Becerra, V. & González, M.I. (2008). Low genetic diversity among garlic (Allium sativum L.) accessions detected using random amplified polymorphic DNA (RAPD). Chilean Journal of Agricultural Research 68(1), 3-12. https://www.scielo.cl/scielo.php?pid=S0718-58392008000100001&script=sci_arttext
Panthee, D.R., KC, Regmi, R.B., Subedi, H.N., Bhattarai, P.P. & Dhakal, J. (2006). Diversity analysis of garlic (Allium sativum L.) germplasms available in Nepal based on morphological characters. Genetic Resources and Crop Evolution. 53(1), 205-212. https://doi.org/10.1007/s10722-004-6690-z
Petropoulos, S. A., Fernandes, A., Ntatsi, G., Petrotos, K., Barros, L., & Ferreira, C. F. R. (2018). Nutritional Value, chemical characterization and bulb morphology of greek garlic landraces. Molekules, 23(319), 1-14. https://doi.org/10.3390/ molecules23020319
Polyzos, N., Papasotiropoulos, V., Lamari, F.N., Petropoulos, S.A. & Bebeli, P.J. (2019). Phenotypic characterization and quality traits of Greek garlic (Allium sativum L.) germplasm cultivated at two different locations. Genetic Resources and Crop Evolution, 66(8), 1671-1689. https://doi.org/10.1007/ s10722-019-00831-4
Shaaf, S., Sharma, R., Kilian, B., Walther, A., Özkan, H., Karami, E. & Mohammadi, B. (2014). Genetic structure and eco-geographical adaptation of garlic landraces (Allium sativum L.) in Iran. Genetic Resources and Crop Evolution, 61(8), 1565-1580. https://doi.org/10.1007/s10722-014-0131-4
Takım, K. & Kutlu, T. (2020). Determination of phytochemical profile of Allium tuncelianum and evaluation of its antiproliferative effect on various human cell lines. KSU J. Agric Nat 23 (1), 259-270, DOI:10.18016/ksutarimdoga.vi.586805.
TÜİK (2022). Bitkisel Üretim İstatistikleri, Sarımsak Üretim Verileri, Https://Biruni.Tuik.Gov.Tr/ Medas/?Kn=92andlocale=Tr (Erişim Tarihi 17.02.2022)
Untergasser, A. (2008). DNA Miniprep using CTAB untergasser’s lab. Summer 2008. (include here the date when you accessed these page). <http://www.untergasser.de/lab/protocols/miniprep_dna_ctab_v1_0.htm>.
UPOV (2001). http://www.upov.int/edocs/ tgdocs/en/ tg162.
Wang, H., Li, X., Shen, D., Oiu, Y. & Song, J. (2014). Diversity evaluation of morphological traits and allicin content in garlic (Allium sativum L.) from China. Euphytica, 198(2), 243-254. https://doi.org/ 10.1007/s10681-014-1097
Volk, G.M. & Stern, D. (2009). Phenotypic characteristics of ten garlic cultivars grown at different North American locations. HortScience. 44(5), 1238- 1247. https://doi.org/10.21273/ HORTSCI.44.5.1238
Vural, H., Eşiyok, D., & Duman, İ. (2000). Kültür Sebzeleri (Sebze Yetiştirme). Ege Üniversitesi, Ziraat Fakültesi, Bornova, İzmir, 440 s.
Yarali Karakan, F. (2022). Relationship between volatile sulfur compounds, mineral content, morphological and molecular characterızatıon of local garlic genotypes. Bangladesh J. Bot. 51(1), 147-155. https://doi.org/10.3329/bjb.v51i1.58831

Morphological and Molecular Characterization of Garlic (Allium sativum L.) Genotypes Growing in Altınözü District

Yıl 2024, , 333 - 343, 01.04.2024

Faika Yaralı Karakan , Ayhan Kılıç , Berna Ergun Çetin

https://doi.org/10.18016/ksutarimdoga.vi.1324022

Cited By: 2

Öz

This study was carried out to determine the morphological characteristics and molecular characterization of some garlic genotypes grown in Altınözü district of Hatay province. As a result of the research, the Mayadalı genotype stood out in terms of plant height, pseudostem diameter, pseudostem length, leaf length, leaf width, bulb weight, bulb length, bulb width and clove weight while genotype Çetenli had a high value of a number of cloves. The ground color of the bulbs was white and white-yellowish, the base shape of the bulbs was recessed and rounded, the cloves color of flesh was yellowish and white, the distribution of cloves was regular and irregular, the color of the cloves crust was pink, purple and brown. Dendrogram based on UPGMA method grouped the garlic genotypes into two main clusters. It is thought that the genetic diversity determined in the research, which is important in terms of revealing the originality of garlic genotypes grown in Altınözü district, will contribute to the development of new garlic cultivars.

Anahtar Kelimeler

Allium sativum L, Garlic, Molecular characterization, Morphological characterization, SSR

Kaynakça

Akan, S. (2022). Morphological characterisation and volatile analysis of Turkish garlic genotypes. Turkish Journal of Agriculture and Forestry, 46(4), 424-440.
Akan, S., Horzum, Ö. & Tuna Güneş, N. (2019). Modifiye atmosferli paketlemenin yeşil sarımsak (Allium sativum L.) yapraklarında kalite korunumuna etkisi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 23(4),165-173. https://dergipark.org.tr/tr/download/article-file/890357
Ayed, C., Bayoudh, C., Rhimi, A., Mezghani, N., Haouala, F. & Dridi, B.A.M. (2018). In vitro propagation of tunisian local garlic (Allium Sativum L.) from shoot-tip culture. Journal of Horticulture and Postharvest Research, 1(2), 75-86. DOI: 10.22077/jhpr.2018.1457.1016. 10.22077/ JHPR.2018.1457.1016
Ayres, N.M., Mcclung, A.M., Larkin, P.D., Bligh, H.F.J., Jones, C.A. & Park, W.D. (1997). Microsatellite and a single nucleotide polymorphism differentiate apparent amylose classes in an extended pedigree of US rice germplasm. Theoretical and Applied Genetics, 94,773- 781. https://doi.org/10.1007/s001220050477
Barandiaran, X., Martin, N., Alba, C., Rodriguez-Conde, M.F., Pietro, A.D. & Martin, J. (1999). An efficient method for the ın vitro management of multiple garlic accessions. In Vitro Cell. Dev. Biol. Plant., 35, 466-469. https://doi.org/10.1007/s11627-999-0069-y
Beşirli, G., İnan, Y. & Türkeş, T. (1994). Sarımsak çeşit tespit denemesi, bilimsel araştırma ve incelemeler. Atatürk Bahçe Kültürleri Merkez Araştırma Enstitüsü, Yayın No: 41, 14 s.,Yalova.
Buso, G.S.C., Paiva, M.R., Torres, A.C., Resende, F.V., Ferreira, M.A., Buso, J.A. & Dusi, A.N. (2008). Genetic diversity studies of Brazilian garlic cultivars and quality control of garlic-clover production. Genetics and Molecular Research, 7(2), 534-541. DOI: 10.4238/vol7-2gmr451
De-Oliveira, J.T., De-Oliveira, R.A., Cunha, F.F., Silva Ribeiro, I., Oliveira, L.A.A. & Teodoro, P.E. (2020). Contribution of morphological variables in garlic bulb yield. HortScience, 55(6), 896-897. https://doi.org/10.21273/HORTSCI14996-20
Doyle, J.J. & Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus,12: 13-15.
Etoh, T., & Simon, P.W. (2002). Diversity, fertility and seed production of garlic. in: Rabinowitch H.D. & Currah L. (eds) Allium Crop Science Recent Advances. CABI Publishing, Wallingford, UK, 101–117p.
Figliuolo, G., Candido, V., Logozzo, G., Miccolis, V. & Zeuli, P.S. (2001). Genetic evaluation of cultivated garlic germplasm Allium sativum L. and A. Ampeloprasum L. Euphytica. 121(3): 325-334. https://doi.org/10.1023/A:1012069532157
Garcia-Vallve, S., Palau J. & Romeu, A. (1999). Horizontal gene transfer in glycosyl hydrolases inferred from codon usage in Escherichia coli and Bacillus subtilis. Molecular Biology and Evololution, 16(9):1125-1134.
Geboloğlu, N., Karabekiroğlu, D.S., & Doksöz, S. (2017). Tokat sarımsağının morfolojik ve moleküler karekterizasyonu. Akademik Ziraat Dergisi, 6: 131-136. https://app.trdizin.gov.tr/makale/ TWpVMk 16SXlNZz09
Günaydın, Ö. (2011). Sarımsakta (Allium sativum L.) düşük sıcaklıkta depolama uygulamasından farklı etkilenen aday genlerin cDNA-AFLP yöntemi ile belirlenmesi (Tez no 284803). [Yüksek Lisans Tezi, Uludağ Üniversitesi, Fen Bilimleri Enstitüsü, Bahçe Bitkileri Ana Bilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
Jo, M.H., Ham, I.K., Moe, K.T., Kwon, S.W., Lu, F.H., Park, Y.J. & Lee, E.M. (2012). Classification of genetic variation in garlic (Allium sativum L.) using SSR markers. Australian Journal of Crop Science, 6(4), 625-631. https://search.informit.org/doi/ 10.3316/ informit.362158671579385
Khar, A., Asha Devi, A., Mahajan, V. & Lawande, K.E. (2006). Genetic divergence analysis in elite lines of garlic (Allium sativum L.). J. Maha. Agri. Univ., 31, 52- 55
Kıraç, H. (2019). Türkiye'de yaygın olarak yetiştiriciliği yapılan farklı sarımsak genotiplerinin morfolojik ve moleküler karakterizasyonu (Tez no: 560519). [Yüksek Lisans Tezi, Erciyes Üniversitesi Fen Bilimleri Enstitüsü Bahçe Bitkileri Anabilim Dalı]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
Kumar, M., Sharma, V. R., Kumar, V., Sirohi, U., Chaudhary, V., Sharma, S. & Sharma, S. (2019). Genetic diversity and population structure analysis of Indian garlic (Allium sativum L.) collection using SSR markers. Physiology and Molecular Biology of Plants, 25(2), 377-386. https://doi.org/10.1007/ s12298-018-0628-y
Manjunathagowda, D.C., Gopal, J. Archana, R. & Asiya, K.R. (2017). Virus–free seed production of garlic (Allium sativum L.): status and prospects. Int.J.Curr.Microbiol.App.Sci., 6(6), 2446-2456. https://doi.org/10.20546/ijcmas.2017.606.290
Paredes, M., Becerra, V. & González, M.I. (2008). Low genetic diversity among garlic (Allium sativum L.) accessions detected using random amplified polymorphic DNA (RAPD). Chilean Journal of Agricultural Research 68(1), 3-12. https://www.scielo.cl/scielo.php?pid=S0718-58392008000100001&script=sci_arttext
Panthee, D.R., KC, Regmi, R.B., Subedi, H.N., Bhattarai, P.P. & Dhakal, J. (2006). Diversity analysis of garlic (Allium sativum L.) germplasms available in Nepal based on morphological characters. Genetic Resources and Crop Evolution. 53(1), 205-212. https://doi.org/10.1007/s10722-004-6690-z
Petropoulos, S. A., Fernandes, A., Ntatsi, G., Petrotos, K., Barros, L., & Ferreira, C. F. R. (2018). Nutritional Value, chemical characterization and bulb morphology of greek garlic landraces. Molekules, 23(319), 1-14. https://doi.org/10.3390/ molecules23020319
Polyzos, N., Papasotiropoulos, V., Lamari, F.N., Petropoulos, S.A. & Bebeli, P.J. (2019). Phenotypic characterization and quality traits of Greek garlic (Allium sativum L.) germplasm cultivated at two different locations. Genetic Resources and Crop Evolution, 66(8), 1671-1689. https://doi.org/10.1007/ s10722-019-00831-4
Shaaf, S., Sharma, R., Kilian, B., Walther, A., Özkan, H., Karami, E. & Mohammadi, B. (2014). Genetic structure and eco-geographical adaptation of garlic landraces (Allium sativum L.) in Iran. Genetic Resources and Crop Evolution, 61(8), 1565-1580. https://doi.org/10.1007/s10722-014-0131-4
Takım, K. & Kutlu, T. (2020). Determination of phytochemical profile of Allium tuncelianum and evaluation of its antiproliferative effect on various human cell lines. KSU J. Agric Nat 23 (1), 259-270, DOI:10.18016/ksutarimdoga.vi.586805.
TÜİK (2022). Bitkisel Üretim İstatistikleri, Sarımsak Üretim Verileri, Https://Biruni.Tuik.Gov.Tr/ Medas/?Kn=92andlocale=Tr (Erişim Tarihi 17.02.2022)
Untergasser, A. (2008). DNA Miniprep using CTAB untergasser’s lab. Summer 2008. (include here the date when you accessed these page). <http://www.untergasser.de/lab/protocols/miniprep_dna_ctab_v1_0.htm>.
UPOV (2001). http://www.upov.int/edocs/ tgdocs/en/ tg162.
Wang, H., Li, X., Shen, D., Oiu, Y. & Song, J. (2014). Diversity evaluation of morphological traits and allicin content in garlic (Allium sativum L.) from China. Euphytica, 198(2), 243-254. https://doi.org/ 10.1007/s10681-014-1097
Volk, G.M. & Stern, D. (2009). Phenotypic characteristics of ten garlic cultivars grown at different North American locations. HortScience. 44(5), 1238- 1247. https://doi.org/10.21273/ HORTSCI.44.5.1238
Vural, H., Eşiyok, D., & Duman, İ. (2000). Kültür Sebzeleri (Sebze Yetiştirme). Ege Üniversitesi, Ziraat Fakültesi, Bornova, İzmir, 440 s.
Yarali Karakan, F. (2022). Relationship between volatile sulfur compounds, mineral content, morphological and molecular characterızatıon of local garlic genotypes. Bangladesh J. Bot. 51(1), 147-155. https://doi.org/10.3329/bjb.v51i1.58831

Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	Türkçe
Konular	Sebze Yetiştirme ve Islahı
Bölüm	ARAŞTIRMA MAKALESİ (Research Article)
Yazarlar	Faika Yaralı Karakan 0000-0002-2176-8663 Ayhan Kılıç 0000-0002-8661-7268 Berna Ergun Çetin 0000-0002-6399-0916
Erken Görünüm Tarihi	21 Ocak 2024
Yayımlanma Tarihi	1 Nisan 2024
Gönderilme Tarihi	7 Temmuz 2023
Kabul Tarihi	12 Ekim 2023
Yayımlandığı Sayı	Yıl 2024

Kaynak Göster

APA	Yaralı Karakan, F., Kılıç, A., & Ergun Çetin, B. (2024). Altınözü İlçesinde Yetiştirilen Sarımsak (Allium sativum L.) Genotiplerinin Morfolojik ve Moleküler Karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(2), 333-343. https://doi.org/10.18016/ksutarimdoga.vi.1324022