Öz
Bu çalışmada, kültürü
yapılan 30 pamuk çeşidi (Gossypium
hirsutum L.) arasındaki genetik ilişkinin ISSR yöntemi kullanılarak
belirlenmesi hedeflenmiştir. Otuz pamuk çeşidinde polimorfizmin belirlenmesi
amacıyla 24 ISSR primeri 8 pamuk çeşidinde test edilmiştir. Primerlerin
yalnızca 9 tanesi PCR ürünü oluşturmuş ve sonraki çalışmalar bu primerlerle
sürdürülmüştür. Seçilen 9 adet ISSR primeri 30 adet pamuk çeşidinde toplam 41
bant oluştururken bu bantlardan ortalama 22.3 tanesinin polimorfik olduğu
saptanmış, primer başına polimorfik bant sayısı ortalama 2.5 olarak
gerçekleşmiştir. Araştırmada kullanılan tüm primerler pamukta polimorfik bant
üretirken, polimorfizm oranı primerlere bağlı olarak %6 ile %89 arasında
değişim göstermiştir. ISSR primerlerine ilişkin polimorfik bilgi içeriği
değerleri 0.19 ile 0.68 aralığında değişim göstermiş ve ortalama 0.49 olmuştur.
Çeşitler arası ortalama Jaccard benzerlik katsayısı 0.77 olarak bulunurken,
UPGMA kümeleme analiz sonucu 30 pamuk çeşidi genetik yakınlık açısından 2 ana
kümeye ayrılmıştır.
Anahtar Kelimeler
Teşekkür
Yardımları ve destekleri için Prof.Dr. Nafiz ÇELİKTAŞ, Dr.Öğr.Üyesi Yaşar AKIŞCAN ve Prof.Dr. Hakan ÖZKAN’a teşekkür ediyoruz. Bu çalışma, Cenk Burak ŞAHİN’in yüksek lisans tezinden üretilmiştir. Çalışmanın özeti, “1st International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem’18)” kongresinde poster bildiri olarak sunulmuştur
Kaynakça
- Bardak A, Bolek Y 2012. Genetic diversity of diploid and tetraploid cottons determined by SSR and ISSR markers. Turkish Journal of Field Crops, 17(2):139-144
- Belaj A, Satovic Z, Cdpriani G, Baldoni L, Testolev R., Rallo L, Trujtllo I 2003. Comparative study of the discriming capacity of RAPD, AFLP and SSR markers and of their effectiveness inestablishing genetic relationships in olive. Theoretical and Applied Genetics, 107(4): 736-744.
- Cardy BJ, Beversdorf WD 1984. A procedure for the starch gel electrophoretic detection of isozymes in soybean [Glycine max (L.) Merr.]. Dep. Crop Sci. Tech. Bull 119/8401. Univ. of Guelph, Ontario, Canada.
- Chaudhary L, Sindhu A, Kumar M, Kumar R, Saini M 2010. Estimation of genetic divergence among some cotton varieties by RAPD analysis. In Journal of Plant Breeding and Crop Science, 2:39-43.
- Doyle JJ, Doyle JL 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissues. Phytochemical Bulletin, 19(1): 11-15.
- Erkılınç A, Karaca M 2005. Assessment of Genetic Variation in Some Cotton Varieties (Gossypium hirsutum L.) Grown in Turkey Using Microsatellite.
- FAO 2018. Food and Agriculture Organization of the United Nations. http://www.fao.org Erişim Tarihi: 27.12.2018
- Gorman MB, Kiang YT 1977. Variety-specific electrophoretic variants of four soybean enzymes. Crop Science 17: 963-965
- Hamrick JL, Godt MJW, Murawski DA, Loveless MD 1991. Correlations between species traits and allozyme diversity: implications for conservation biology. In: Genetic and conservation of rare plants (Falk DA and Holsinger KE, eds.). Oxford University Press, New York, 75-86.
- Hussein EHA, Mohamed AA, Attia S, Adawy SS 2006. Molecular characterization and genetic relationships among cotton genotypes 1- RAPD, ISSR and SSR analysis. Arab Journal of Biotechnology, 9: 313-328.
- Kwon YS, Ryu TH, Kim CH, Song ICH, Kim KM 2004. A Comparative Study of the RAPD and SSR Markers in Establishing a Genetic Relationship of the Various Types of Cucurbita. Korean Journal of Genetics, 26 (2): 115-122.
- Liu B, Wendel JF 2001. Intersimple sequence repeat (ISSR) polymorphisms as a genetic marker system in cotton. Molecular Ecology Notes, 1(3): 205-208.
- Mert, M., 2009. Lif Bitkileri. Nobel Yayın Dağıtım, 1446, 278 s, Ankara.
- Mignouna HD, Abanf MM, Fagbemi SA 2003. A Comparative Assessment of molecular marker assays (AFLP, RAPD and SSR) for White yam (Dioscirea rotundata Poir )germ plasm characterisation. Annals of Applied Biology, 142 :269-276.
- Nei M 1972. Genetic distance between populations. American Naturalist, 106: 283-292
- Nei M, Li WH 1979. Mathematical model for studying variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci., 76: 5269-5273.
- Peirce LC, Brewbaker JL 1973. Applications of isozyme analysis in horticultural science. Hort. Science, 8: 17-22
- Pillay M, Myers GO 1999. Genetic diversity in cotton assessed by variation in ribosomal RNA genes and AFLP markers. Crop Science, November-December. 39:1881-1886.
- Rana MK, Bhat KV 2004. A Comparison of AFLP and RAPD Markers for Genetic Diversity and Cultivar Identification in Cotton. J. Plant Biochemistry & Biotechnology, 13: 19-24.
- Reinisch AJ, Dong JM, Brubaker CL, Stelly DM, Wendel JF, Paterson AH 1994. A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome. Genetics, 138:829-847.
- Sammour RH 1991. Using electrophoretic techniques in varietal identification, biosystematic analysis, phylogenetic relations and genetic resources management. J. Islamic Acad. Sci., 4: 221-226.
- Tanksley SD, Young ND, Peterson AH, Bonierbale MW 1989. RFLP mapping in plant breeding:new tools for old sciences. Biotechnology, 7:257-264.
- TTSM 2018. Tohumluk Tescil ve Sertifikasyon Merkez Müdürlüğü. http://www.ttsm.gov.tr Erişim Tarihi: 27.12.2018
- TÜİK 2018. Türkiye İstatistik Kurumu. http://www.tuik.gov.tr Erişim Tarihi: 27.12.2018
- Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531–6535.
- Zietkiewicz E, Rafalski A, Labuda D 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20(2):176-183
Öz
This study was conducted to
determine the genetic diversity in 30 cotton (Gossypium hirsutum L.) cultivars extensively cultivated in Turkey
by using ISSR DNA molecular markers. To investigate the genetic diversity in 30
cotton varieties by ISSR molecular marker, 24 ISSR primers were screened in 8
varieties. Overall, 9 of 24 which produced a PCR product were selected
according their polymorphism level. These ISSR primers totally produced 41
bands, and 22.3 were polymorphic. The percentage of polymorphic bands per
primer was detected as 2.5. The rate of polymorphism depending on the primers
ranged between 6% and 89%. Average polymorphism information content was 0.49,
with minimum PIC 0.19 and maximum PIC 0.68. While the Jaccard similarity
coefficient between the genotypes was detected as 0.77, 30, cotton varieties
were grouped within two main clusters in respect to genetic similarity based on
UPGMA analyses.
Anahtar Kelimeler
Kaynakça
- Bardak A, Bolek Y 2012. Genetic diversity of diploid and tetraploid cottons determined by SSR and ISSR markers. Turkish Journal of Field Crops, 17(2):139-144
- Belaj A, Satovic Z, Cdpriani G, Baldoni L, Testolev R., Rallo L, Trujtllo I 2003. Comparative study of the discriming capacity of RAPD, AFLP and SSR markers and of their effectiveness inestablishing genetic relationships in olive. Theoretical and Applied Genetics, 107(4): 736-744.
- Cardy BJ, Beversdorf WD 1984. A procedure for the starch gel electrophoretic detection of isozymes in soybean [Glycine max (L.) Merr.]. Dep. Crop Sci. Tech. Bull 119/8401. Univ. of Guelph, Ontario, Canada.
- Chaudhary L, Sindhu A, Kumar M, Kumar R, Saini M 2010. Estimation of genetic divergence among some cotton varieties by RAPD analysis. In Journal of Plant Breeding and Crop Science, 2:39-43.
- Doyle JJ, Doyle JL 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissues. Phytochemical Bulletin, 19(1): 11-15.
- Erkılınç A, Karaca M 2005. Assessment of Genetic Variation in Some Cotton Varieties (Gossypium hirsutum L.) Grown in Turkey Using Microsatellite.
- FAO 2018. Food and Agriculture Organization of the United Nations. http://www.fao.org Erişim Tarihi: 27.12.2018
- Gorman MB, Kiang YT 1977. Variety-specific electrophoretic variants of four soybean enzymes. Crop Science 17: 963-965
- Hamrick JL, Godt MJW, Murawski DA, Loveless MD 1991. Correlations between species traits and allozyme diversity: implications for conservation biology. In: Genetic and conservation of rare plants (Falk DA and Holsinger KE, eds.). Oxford University Press, New York, 75-86.
- Hussein EHA, Mohamed AA, Attia S, Adawy SS 2006. Molecular characterization and genetic relationships among cotton genotypes 1- RAPD, ISSR and SSR analysis. Arab Journal of Biotechnology, 9: 313-328.
- Kwon YS, Ryu TH, Kim CH, Song ICH, Kim KM 2004. A Comparative Study of the RAPD and SSR Markers in Establishing a Genetic Relationship of the Various Types of Cucurbita. Korean Journal of Genetics, 26 (2): 115-122.
- Liu B, Wendel JF 2001. Intersimple sequence repeat (ISSR) polymorphisms as a genetic marker system in cotton. Molecular Ecology Notes, 1(3): 205-208.
- Mert, M., 2009. Lif Bitkileri. Nobel Yayın Dağıtım, 1446, 278 s, Ankara.
- Mignouna HD, Abanf MM, Fagbemi SA 2003. A Comparative Assessment of molecular marker assays (AFLP, RAPD and SSR) for White yam (Dioscirea rotundata Poir )germ plasm characterisation. Annals of Applied Biology, 142 :269-276.
- Nei M 1972. Genetic distance between populations. American Naturalist, 106: 283-292
- Nei M, Li WH 1979. Mathematical model for studying variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci., 76: 5269-5273.
- Peirce LC, Brewbaker JL 1973. Applications of isozyme analysis in horticultural science. Hort. Science, 8: 17-22
- Pillay M, Myers GO 1999. Genetic diversity in cotton assessed by variation in ribosomal RNA genes and AFLP markers. Crop Science, November-December. 39:1881-1886.
- Rana MK, Bhat KV 2004. A Comparison of AFLP and RAPD Markers for Genetic Diversity and Cultivar Identification in Cotton. J. Plant Biochemistry & Biotechnology, 13: 19-24.
- Reinisch AJ, Dong JM, Brubaker CL, Stelly DM, Wendel JF, Paterson AH 1994. A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome. Genetics, 138:829-847.
- Sammour RH 1991. Using electrophoretic techniques in varietal identification, biosystematic analysis, phylogenetic relations and genetic resources management. J. Islamic Acad. Sci., 4: 221-226.
- Tanksley SD, Young ND, Peterson AH, Bonierbale MW 1989. RFLP mapping in plant breeding:new tools for old sciences. Biotechnology, 7:257-264.
- TTSM 2018. Tohumluk Tescil ve Sertifikasyon Merkez Müdürlüğü. http://www.ttsm.gov.tr Erişim Tarihi: 27.12.2018
- TÜİK 2018. Türkiye İstatistik Kurumu. http://www.tuik.gov.tr Erişim Tarihi: 27.12.2018
- Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531–6535.
- Zietkiewicz E, Rafalski A, Labuda D 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20(2):176-183
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Ziraat, Veterinerlik ve Gıda Bilimleri |
| Bölüm | ARAŞTIRMA MAKALESİ (Research Article) |
| Yazarlar | |
| Yayımlanma Tarihi | 28 Şubat 2020 |
| Gönderilme Tarihi | 20 Mayıs 2019 |
| Kabul Tarihi | 5 Temmuz 2019 |
| Yayımlandığı Sayı | Yıl 2020 |
Cited By
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