Pleurotus eryngii’ nin Misel Biyokütlesinin ve Farklı Olgunlaşma Seviyesindeki Gövdelerinin Ekstraksiyonunun Optimizasyonu ve Antidiyabetik Özelliklerinin Belirlenmesi
Öz
Yenilebilir mantarlar, tarih öncesi zamanlardan beri küresel olarak tüketilen bir gıda maddesidir. Mantarların bu nama sahip olmalarında duyusal özellikleri başta olmak üzere, besleyici değeri ve tıbbi özellikleri etkili olmuştur. Ancak ülkemiz bazında bakıldığında yenilebilir mantarlar üretim, tüketim ve bilimsel çalışmalar açısından yeterli düzeye ulaşamamıştır. Bu çalışmada, Pleurotus eryngii mantarının yaşam döngüsünden farklı parçalarının ekstraksiyon koşullarının optimizasyonu, Yanıt Yüzey Yöntemi (YYY) esas alınarak araştırılmıştır. Optimize edilmiş ekstraktların toplam fenolik madde miktarı (TPC), antioksidan ve antidiyabetik etkileri incelenmiştir. Ektrasksiyon parametleri olan; sıcaklık, sıvı / katı oranı ve solvent konsantrasyonu, örneklerin TPC ve antioksidan kapasitelerini önemli ölçüde etkilemiştir. Mantarların genç meyve gövdesi miselyum biyokütlesine göre daha yüksek antioksidan ve antidiyabetik aktivite göstermiştir. Ancak meyve gövdesi yaşlandıkça bu etkiler kısmen azalmıştır. Sonuç olarak mantarların hem meyve gövdesi hem de miselyum biyokütlesi birçok biyo-fonksiyonel özelliğe sahiptir. Miselyum biyokütlesinin üretim süresinin kısa oluşu ancak aktivitesinin meyve gövdesine göre azlığı değerlendirilmesi gereken konulardan biri olup, hasat zamanının gecikmesi ile de bu özellikleri önemli ölçüde azalması yadsınamaz bir gerçektir. Bu çalışma ile P.eryngii mantarının doğal antioksidan ve anti-diyabetik gıda olma potansiyeli ortaya konulmuştur.
Anahtar Kelimeler
Pleurotus eryngii Miselyum biyokütle Antioksidian antidiyabetik YYY
Destekleyen Kurum
Yozgat Bozok Üniversitesi Proje Koordinasyon Uygulama ve Araştırma Merkezi
Proje Numarası
6602b/BMYO/17-125
Teşekkür
Bu çalışmaya 6602b/BMYO/17-125 numaralı Bilimsel Araştırma Projeleri ile destek sağlayan Yozgat Bozok Üniversitesi Proje Koordinasyon Uygulama ve Araştırma Merkezi’ne teşekkürlerimi sunarım.
Kaynakça
- Arora, D. (2008). Notes on economic mushrooms. Econ. Bot, 62, 540-544.
- Arora, D., ve Shepard, G. H. (2008). Mushrooms and economic botany. Economic Botany, 62(3), 207-212.
- Atila, F., Tuzel, Y., Fernández, J. A., Cano, A. F., ve Sen, F. (2018). The effect of some agro–industrial wastes on yield, nutritional characteristics and antioxidant activities of Hericium erinaceus isolates. Scientia Horticulturae, 238, 246-254.
- Barwick, V. J. (1997). Strategies for solvent selection—a literature review. TrAC Trends in Analytical Chemistry, 16(6), 293-309.
- Benzie, I., ve Strain, J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of" antioxidant power". Anal Biochem. 1996: the FRAP assay, 15(239), 1.
- Bowman, S., ve Free, S. (2006). The structure and synthesis of the fungal cell wall. Bioassays, 28: 799–808.
- Brand-Williams, B., Cuvelier, M., & Berset, C. (1995). Review: Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int, 28, 25-30.
- Cam, M., Basyigit, B., Alasalvar, H., Yilmaztekin, M., Ahhmed, A., Sagdic, O., . . . ve Telci, I. (2020). Bioactive properties of powdered peppermint and spearmint extracts: Inhibition of key enzymes linked to hypertension and type 2 diabetes. Food Bioscience, 100577.
- Çam, M., Hışıl, Y., ve Durmaz, G. (2009). Classification of eight pomegranate juices based on antioxidant capacity measured by four methods. Food chemistry, 112(3), 721-726.
- Cohen, R., Persky, L., ve Hadar, Y. (2002). Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Applied microbiology and biotechnology, 58(5), 582-594.
- Daba, A. S., Kabeil, S., Botros, W. A., ve El-Saadani, M. (2008). Production of mushroom (Pleurotus ostreatus) in Egypt as a source of nutritional and medicinal food. World J. Agric. Sci, 4(5), 630-634.
- Daba, A., ve Ezeronye, O. (2003). Anti-cancer effect of polysaccharides isolated from higher basidiomycetes mushrooms. African Journal of Biotechnology, 2(12), 672-678.
- De Silva, D. D., Rapior, S., Hyde, K. D., ve Bahkali, A. H. (2012). Medicinal mushrooms in prevention and control of diabetes mellitus. Fungal Diversity, 56(1), 1-29.
- Dent, M., Dragović-Uzelac, V., Penić, M., Bosiljkov, T.,ve Levaj, B. (2013). The effect of extraction solvents, temperature and time on the composition and mass fraction of polyphenols in Dalmatian wild sage (Salvia officinalis L.) extracts. Food technology and biotechnology, 51(1), 84-91.
- Erkan, E., ve Pekşen, A. (2019). Türkiye’de Kültür Mantarı Üretimi ve Teknolojik Gelişmeler. Mantar Dergisi, 10(3), 225-233. Friedman, J., Hastie, T., ve Tibshirani, R. (2001). The elements of statistical learning (Vol. 1): Springer series in statistics New York.
- Gunde-Cimerman, N. (1999). Medicinal value of the genus Pleurotus (Fr.) P. Karst.(agaricales sl, Basidiomycetes). International Journal of Medicinal Mushrooms, 1(1).
- Jablonsky, I., Copikova, J., Blafkova, P., Mickova, K., Synytsya, A., Cerna, J., ve Erban, V. (2005). Isolation and Characterisation of β-Glucans from Fruit Bodies of Pleurotus Strains. http.
- Kris-Etherton, P. M., Hecker, K. D., Bonanome, A., Coval, S. M., Binkoski, A. E., Hilpert, K. F., . . . ve Etherton, T. D. (2002). Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. The American journal of medicine, 113(9), 71-88.
- Lambert, J. D., ve Elias, R. J. (2010). The antioxidant and pro-oxidant activities of green tea polyphenols: a role in cancer prevention. Archives of biochemistry and biophysics, 501(1), 65-72.
- Lebovitz, H. E. (1995). A new oral therapy for diabetes management: alpha-glucosidase inhibition with acarbose. Clinical diabetes, 13(6), 99-104.
- Lee, J. S., Cho, J. Y., Hong, E. K. (2009). Study on macrophage activation and structural characteristics of purified polysaccharides from the liquid culture broth of Hericium erinaceus. Carbohydrate polymers, 78(1), 162-168.
- Litwinienko, G., ve Ingold, K. (2003). Abnormal solvent effects on hydrogen atom abstractions. 1. The reactions of phenols with 2, 2-diphenyl-1-picrylhydrazyl (dpph•) in alcohols. The Journal of organic chemistry, 68(9), 3433-3438. Liyana-Pathirana, C. (2005). Optimization of extraction of phenolics compounds from wheat using response surface methodology. Food Chem., 93, 45-46.
- Manzi, P., Aguzzi, A., Vivanti, V., Paci, M., ve Pizzoferrato, L. (1999). Mushrooms as a source of functional ingredients, Euro Food Chemistry, 10. Paper presented at the Europien Conference on: Functional foods, a new challenge fort the food chemist, Budapest.
- Markom, M., Hasan, M., Daud, W. R. W., Singh, H., ve Jahim, J. M. (2007). Extraction of hydrolysable tannins from Phyllanthus niruri Linn.: Effects of solvents and extraction methods. Separation and purification technology, 52(3), 487-496.
- McDougall, G. J., Shpiro, F., Dobson, P., Smith, P., Blake, A., ve Stewart, D. (2005). Different polyphenolic components of soft fruits inhibit α-amylase and α-glucosidase. Journal of agricultural and food chemistry, 53(7), 2760-2766.
- Miles, P. G., ve Chang, S.-T. (2004). Mushrooms: cultivation, nutritional value, medicinal effect, and environmental impact: CRC press.
- Myers, R. H., Montgomery, D. C., ve Anderson-Cook, C. M. (1995). Response Surface Methodology: Process and Product Optimization Using Designed Experiments, John Wiley & Sons. Inc., New York, NY, 134-174.
- Natrella, M. (2010). NIST/SEMATECH e-handbook of statistical methods. Nist/Sematech, 49.
- Ohga, S., ve Royse, D. J. (2004). Cultivation of Pleurotus eryngii on umbrella plant (Cyperus alternifolius) substrate. Journal of Wood Science, 50(5), 466-469.
- Öztürk, A., ve Çopur, Ö. U. (2009). Mantar bileşenlerinin teröpatik etkileri. Bahçe, 38(1), 19-24.
- Sanmee, R., Dell, B., Lumyong, P., Izumori, K., ve Lumyong, S. (2003). Nutritive value of popular wild edible mushrooms from northern Thailand. Food chemistry, 82(4), 527-532.
- Sarangi, I., Ghosh, D., Bhutia, S. K., Mallick, S. K.,ve Maiti, T. K. (2006). Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans. International immunopharmacology, 6(8), 1287-1297.
- Singh, R., ve Mishra, K. (2008). Mushroom cultivation.
- Singleton, V. L., Orthofer, R., ve Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology, 299, 152-178.
- Snyder, L. (1974). Classification of the solvent properties of common liquids. Journal of Chromatography A, 92(2), 223-230.
- Stamets, P. (2011). Growing gourmet and medicinal mushrooms: Ten speed press.
- Stojkovic, D., Smiljkovic, M., Ciric, A., Glamoclija, J., Van Griensven, L., Ferreira, I. C., ve Sokovic, M. (2019). An insight into antidiabetic properties of six medicinal and edible mushrooms: Inhibition of α-amylase and α-glucosidase linked to type-2 diabetes. South African Journal of Botany, 120, 100-103.
- Sturion, G., ve Oetterer, M. (1995). Utilization of banana leaves as substrate on edible mushroom cultivation (Pleurotus spp.). Ciencia e Technologia de Alimentos, 15(2), 194-200.
- Su, C.-H., Lai, M.-N., ve Ng, L.-T. (2013). Inhibitory effects of medicinal mushrooms on α-amylase and α-glucosidase–enzymes related to hyperglycemia. Food & function, 4(4), 644-649.
- Suleria, H. A. R., Goyal, M. R., ve Butt, M. S. (Eds.). (2019). Phytochemicals from Medicinal Plants: Scope, Applications, and Potential Health Claims. CRC Press.
- Totelin, L. (2015). When foods become remedies in ancient Greece: The curious case of garlic and other substances. Journal of ethnopharmacology, 167, 30-37.
- Vetter, J. (2003). Chemical composition of fresh and conserved Agaricus bisporus mushroom. European Food Research and Technology, 217(1), 10-12.
Optimization of Extraction of Mycelial Biomass and Parts at Different Maturation Levels of Pleurotus eryngii and, Determination of İts Antidiabetic Properties
Öz
Edible mushrooms have been consumed as a global food since prehistoric times. Its sensory properties, its nutritional value, and medicinal properties have been affected in having this famous. However, edible mushrooms could not reach a sufficient level in production, consumption, and scientific studies in our country. In this study, optimization of extraction conditions of parts of the life-cycle of Pleurotus eryngii mushroom was investigated based on Response Surface Method (RSM). Total phenolic content (TPC), antioxidant and antidiabetic effects of optimized extracts were investigated. Extract parameters; temperature, liquid/solid ratio, and solvent concentration significantly affected the TPC and antioxidant capacities of the samples. The young fruit body of mushrooms showed higher antioxidant and antidiabetic activity than mycelium biomass. However, these effects partially decreased as the fruit body ages. As a result, both the fruit body and mycelium biomass have many bio-functional properties. The short production time of mycelium biomass, but the low bioactivity than the fruit body, is one of the issues that needs to be evaluated. This research has shown that P.eryngii mushrooms have potential as natural antioxidants and anti-diabetic food.
Anahtar Kelimeler
Pleurotus eryngii Mycelium biomass Antioxidant anti-diabetic RSM
Proje Numarası
6602b/BMYO/17-125
Kaynakça
- Arora, D. (2008). Notes on economic mushrooms. Econ. Bot, 62, 540-544.
- Arora, D., ve Shepard, G. H. (2008). Mushrooms and economic botany. Economic Botany, 62(3), 207-212.
- Atila, F., Tuzel, Y., Fernández, J. A., Cano, A. F., ve Sen, F. (2018). The effect of some agro–industrial wastes on yield, nutritional characteristics and antioxidant activities of Hericium erinaceus isolates. Scientia Horticulturae, 238, 246-254.
- Barwick, V. J. (1997). Strategies for solvent selection—a literature review. TrAC Trends in Analytical Chemistry, 16(6), 293-309.
- Benzie, I., ve Strain, J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of" antioxidant power". Anal Biochem. 1996: the FRAP assay, 15(239), 1.
- Bowman, S., ve Free, S. (2006). The structure and synthesis of the fungal cell wall. Bioassays, 28: 799–808.
- Brand-Williams, B., Cuvelier, M., & Berset, C. (1995). Review: Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int, 28, 25-30.
- Cam, M., Basyigit, B., Alasalvar, H., Yilmaztekin, M., Ahhmed, A., Sagdic, O., . . . ve Telci, I. (2020). Bioactive properties of powdered peppermint and spearmint extracts: Inhibition of key enzymes linked to hypertension and type 2 diabetes. Food Bioscience, 100577.
- Çam, M., Hışıl, Y., ve Durmaz, G. (2009). Classification of eight pomegranate juices based on antioxidant capacity measured by four methods. Food chemistry, 112(3), 721-726.
- Cohen, R., Persky, L., ve Hadar, Y. (2002). Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Applied microbiology and biotechnology, 58(5), 582-594.
- Daba, A. S., Kabeil, S., Botros, W. A., ve El-Saadani, M. (2008). Production of mushroom (Pleurotus ostreatus) in Egypt as a source of nutritional and medicinal food. World J. Agric. Sci, 4(5), 630-634.
- Daba, A., ve Ezeronye, O. (2003). Anti-cancer effect of polysaccharides isolated from higher basidiomycetes mushrooms. African Journal of Biotechnology, 2(12), 672-678.
- De Silva, D. D., Rapior, S., Hyde, K. D., ve Bahkali, A. H. (2012). Medicinal mushrooms in prevention and control of diabetes mellitus. Fungal Diversity, 56(1), 1-29.
- Dent, M., Dragović-Uzelac, V., Penić, M., Bosiljkov, T.,ve Levaj, B. (2013). The effect of extraction solvents, temperature and time on the composition and mass fraction of polyphenols in Dalmatian wild sage (Salvia officinalis L.) extracts. Food technology and biotechnology, 51(1), 84-91.
- Erkan, E., ve Pekşen, A. (2019). Türkiye’de Kültür Mantarı Üretimi ve Teknolojik Gelişmeler. Mantar Dergisi, 10(3), 225-233. Friedman, J., Hastie, T., ve Tibshirani, R. (2001). The elements of statistical learning (Vol. 1): Springer series in statistics New York.
- Gunde-Cimerman, N. (1999). Medicinal value of the genus Pleurotus (Fr.) P. Karst.(agaricales sl, Basidiomycetes). International Journal of Medicinal Mushrooms, 1(1).
- Jablonsky, I., Copikova, J., Blafkova, P., Mickova, K., Synytsya, A., Cerna, J., ve Erban, V. (2005). Isolation and Characterisation of β-Glucans from Fruit Bodies of Pleurotus Strains. http.
- Kris-Etherton, P. M., Hecker, K. D., Bonanome, A., Coval, S. M., Binkoski, A. E., Hilpert, K. F., . . . ve Etherton, T. D. (2002). Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer. The American journal of medicine, 113(9), 71-88.
- Lambert, J. D., ve Elias, R. J. (2010). The antioxidant and pro-oxidant activities of green tea polyphenols: a role in cancer prevention. Archives of biochemistry and biophysics, 501(1), 65-72.
- Lebovitz, H. E. (1995). A new oral therapy for diabetes management: alpha-glucosidase inhibition with acarbose. Clinical diabetes, 13(6), 99-104.
- Lee, J. S., Cho, J. Y., Hong, E. K. (2009). Study on macrophage activation and structural characteristics of purified polysaccharides from the liquid culture broth of Hericium erinaceus. Carbohydrate polymers, 78(1), 162-168.
- Litwinienko, G., ve Ingold, K. (2003). Abnormal solvent effects on hydrogen atom abstractions. 1. The reactions of phenols with 2, 2-diphenyl-1-picrylhydrazyl (dpph•) in alcohols. The Journal of organic chemistry, 68(9), 3433-3438. Liyana-Pathirana, C. (2005). Optimization of extraction of phenolics compounds from wheat using response surface methodology. Food Chem., 93, 45-46.
- Manzi, P., Aguzzi, A., Vivanti, V., Paci, M., ve Pizzoferrato, L. (1999). Mushrooms as a source of functional ingredients, Euro Food Chemistry, 10. Paper presented at the Europien Conference on: Functional foods, a new challenge fort the food chemist, Budapest.
- Markom, M., Hasan, M., Daud, W. R. W., Singh, H., ve Jahim, J. M. (2007). Extraction of hydrolysable tannins from Phyllanthus niruri Linn.: Effects of solvents and extraction methods. Separation and purification technology, 52(3), 487-496.
- McDougall, G. J., Shpiro, F., Dobson, P., Smith, P., Blake, A., ve Stewart, D. (2005). Different polyphenolic components of soft fruits inhibit α-amylase and α-glucosidase. Journal of agricultural and food chemistry, 53(7), 2760-2766.
- Miles, P. G., ve Chang, S.-T. (2004). Mushrooms: cultivation, nutritional value, medicinal effect, and environmental impact: CRC press.
- Myers, R. H., Montgomery, D. C., ve Anderson-Cook, C. M. (1995). Response Surface Methodology: Process and Product Optimization Using Designed Experiments, John Wiley & Sons. Inc., New York, NY, 134-174.
- Natrella, M. (2010). NIST/SEMATECH e-handbook of statistical methods. Nist/Sematech, 49.
- Ohga, S., ve Royse, D. J. (2004). Cultivation of Pleurotus eryngii on umbrella plant (Cyperus alternifolius) substrate. Journal of Wood Science, 50(5), 466-469.
- Öztürk, A., ve Çopur, Ö. U. (2009). Mantar bileşenlerinin teröpatik etkileri. Bahçe, 38(1), 19-24.
- Sanmee, R., Dell, B., Lumyong, P., Izumori, K., ve Lumyong, S. (2003). Nutritive value of popular wild edible mushrooms from northern Thailand. Food chemistry, 82(4), 527-532.
- Sarangi, I., Ghosh, D., Bhutia, S. K., Mallick, S. K.,ve Maiti, T. K. (2006). Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans. International immunopharmacology, 6(8), 1287-1297.
- Singh, R., ve Mishra, K. (2008). Mushroom cultivation.
- Singleton, V. L., Orthofer, R., ve Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology, 299, 152-178.
- Snyder, L. (1974). Classification of the solvent properties of common liquids. Journal of Chromatography A, 92(2), 223-230.
- Stamets, P. (2011). Growing gourmet and medicinal mushrooms: Ten speed press.
- Stojkovic, D., Smiljkovic, M., Ciric, A., Glamoclija, J., Van Griensven, L., Ferreira, I. C., ve Sokovic, M. (2019). An insight into antidiabetic properties of six medicinal and edible mushrooms: Inhibition of α-amylase and α-glucosidase linked to type-2 diabetes. South African Journal of Botany, 120, 100-103.
- Sturion, G., ve Oetterer, M. (1995). Utilization of banana leaves as substrate on edible mushroom cultivation (Pleurotus spp.). Ciencia e Technologia de Alimentos, 15(2), 194-200.
- Su, C.-H., Lai, M.-N., ve Ng, L.-T. (2013). Inhibitory effects of medicinal mushrooms on α-amylase and α-glucosidase–enzymes related to hyperglycemia. Food & function, 4(4), 644-649.
- Suleria, H. A. R., Goyal, M. R., ve Butt, M. S. (Eds.). (2019). Phytochemicals from Medicinal Plants: Scope, Applications, and Potential Health Claims. CRC Press.
- Totelin, L. (2015). When foods become remedies in ancient Greece: The curious case of garlic and other substances. Journal of ethnopharmacology, 167, 30-37.
- Vetter, J. (2003). Chemical composition of fresh and conserved Agaricus bisporus mushroom. European Food Research and Technology, 217(1), 10-12.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | ARAŞTIRMA MAKALESİ |
| Yazarlar | |
| Proje Numarası | 6602b/BMYO/17-125 |
| Yayımlanma Tarihi | 30 Nisan 2021 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 12 Sayı: 1 |
Uluslararası Hakemli Dergi
Dergimiz, herhangi bir başvuru veya yayımlama ücreti almamaktadır
