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Investigation of Chromium (III) Accumulation of Ceratophyllum demersum L. under In vitro Conditions

Year 2018, Volume: 21 Issue: 3, 277 - 285, 30.05.2018

Muhammet Doğan , Mehmet Karataş Muhammad Aasım

https://doi.org/10.18016/ksudobil.332980

Abstract

This
study presents heavy metal accumulation levels of Ceratophyllum dermersum L. exposed to chromium (Cr) at different
concentrations (0-12 mg/L) and durations (1, 3 and 5 days) and effects of Cr
toxicity on fresh and dry weights, photosynthetic pigmentation (chlorophyll a, chlorophyll b, total chlorophyll and carotenoid), protein contents and lipid
peroxidation. Cr accumulation increased with increasing metal concentration and
exposure time. The highest Cr accumulation value was determined at 12 mg/L Cr
application and after 5 days. Significant reductions in photosynaptic pigment and
protein contents from Cr toxicity were noted. Compared with the control groups,
minimum photosynthetic pigment levels were determined at 12 mg/L Cr after 5
days. The maximum reduction in the protein content was determined to be 35.84%.
Malondialdehyde (MDA) content was measured as a product of lipid peroxidation.
MDA content has also increased in direct proportion to the increase in
concentration and application time (p<0.05).
The maximum MDA level in the plant was measured as 15.58 nmol/g at 12 mg L Cr.

Keywords

Heavy metal Bioaccumulation Cr toxicity Phytoremediation

References

  • Akın M, Akın G 2017. Suyun Önemi, Türkiye’de Su Potansiyeli,Su Havzaları ve Su Kirliliği. Ankara Üniversitesi Dil ve Tarih-Coğrafya Fakültesi Dergisi, 47: 105-118.
  • Ali H, Khan E, Sajad MA 2013. Phytoremediation of Heavy Metals-Concepts and Applications. Chemosphere, 91: 869-881.
  • Al-Ubaidy HJ, Rasheed KA 2015. Phytoremediation of Cadmium in River Water by Ceratophyllum demersum. World Journal Of Experimental Biosciences, 3: 14-17.
  • Arora M, Kiran B, Rani S, Rani A, Kaur B, Mittal N 2008. Heavy Metal Accumulation in Vegetables Irrigated with Water from Different Sources. Food Chemistry, 111: 811-815.
  • Bunluesin S, Kruatrachue M, Pokethitiyook P, Lanza GR, Upatham ES, Soonthornsarathool V 2004. Plant Screening and Comparison of Ceratophyllum demersum and Hydrilla verticillata for Cadmium Accumulation. Bulletin of Environmental Contamination and Toxicology, 73: 591-598.
  • Chen M, Zhang L, Li J, He X, Cai J 2015. Bioaccumulation and Tolerance Characteristics of a Submerged Plant (Ceratophyllum demersum L.) Exposed to Toxic Metal Lead. Ecotoxicology and Environmental Safety, 122: 313-321.
  • Costa M, Klein KB 2006. Toxicity and Carcinogenicity of Chromium Compounds in Humans. Critical Reviews in Toxicology, 36: 155-163.
  • Das S, Goswami S, Talukdar AD 2016. Physiological Responses of Water Hyacinth, Eichhornia crassipes (Mart.) Solms, to Cadmium and Its Phytoremediation Potential. Turkish Journal of Biology, 40: 84-94.
  • Doğan M 2005. Ceratophyllum demersum L.’de Kadmiyum Klorür, Sodyum Klorür ve Bunların Kombinasyonlarının Fizyolojik ve Morfolojik Etkileri. Ç.Ü. Fen Bil. Ens., Biyoloji ABD, Doktora Tezi, 137 s.
  • Duman F, Koca FD 2014. Single and Combined Effects of Exposure Concentration and Duration on Biological Responses of Ceratophyllum demersum L. Exposed to Cr Species. International Journal of Phytoremediation, 16: 1192-1208.
  • Duman F, Şahan S, Ceylan A, Koca FD 2010. Biological Responses of Ceratophyllum demersum L. Exposed to Chromium (Cr+6). SDU Journal of Science (E-Journal), 5: 163-171.
  • Eryılmaz H, İpek Şİ, Çelik BY 2014. Investigation of Water Quality of Borçka Dam Lake (Artvin). Journal of Science and Technology of Dumlupinar University, 33: 1-8.
  • Favas PJC, Pratas J, Varun M, D’Souza R, Paul MS 2014. Phytoremediation of Soils Contaminated with Metals and Metalloids at Mining Areas: Potential of Native Flora.( Envormental Risk Assessment of Soil Contamination, InTech Press: Ed. Hernandez-Soriano MC) 485-517.
  • Gaur JP, Noraho N, Chauhan YS 1994. Relationship Between Heavy Metal Accumulation and Toxicity in Spirodela polyrrhiza L. Schleid. and Azolla pinnata R. Br.. Aquatic Botany, 49: 183-192.
  • Gill RA, Zang L, Ali B, Farooq MA, Cui P, Yang Su, Ali S, Zhou W 2015. Chromium-Induced Physio-Chemical and Ultrastructural Changes in Four Cultivars of Brassica napus L.. Chemosphere, 120: 154-164.
  • Heath RL, Packer L 1968. Photoperoxidation in Isolated Chloroplasts I. Kinetic and Stoichiometry of Fatty Acid Peroxidation. Archives of Biochemistry and Biophysics, 125: 189-198.
  • Hoagland DR, Arnon DI 1950. The Water-Culture Method for Growing Plants without Soil. California Agricultural Experiment Station, Circular, 347: 1-32.
  • Kara Y, Zeytunluoglu A 2007. Bioaccumulation of Toxic Metals (Cd and Cu) by Groenlandia densa (L.) Fourr.. Bulletin of Environmental Contamination and Toxicology, 79: 609-612.
  • Koca FD 2012. Cr(III) ve Cr(VI) Maruziyetinde Ceratophyllum demersum L.’nin Biyolojik Cevabı: Konsantrasyon ve Uygulama Periyodunun Etkisi. E.Ü., Fen Bil. Ens., Biyoloji ABD, Yüksek Lisans Tezi, 75 s.
  • Lichtenthaler HK, Wellburn AR 1983. Determinations of Total Carotenoids and Chlorophylls a and b of Leaf Extracts in Different Solvents. Biochemical Society Transactions, 11: 591-592.
  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ, 1951. Protein Measurement with Folin Phenol Reagent. Journal of Biological Chemistry, 193: 265-275.
  • Memon AR, Schröder P 2009. Implications of Metal Accumulation Mechanisms to Phytoremediation. Environmental Science and Pollution Research, 16: 162-175.
  • Mishra S, Srivastava S, Tripathi RD, Kumar R, Seth CD, Gupta DK 2006. Lead Detoxification by Coontail (Ceratophyllum demersum L.) Involves Induction of hytochelatins and Antioxidant System in Response to Its Accumulation. Chemosphere, 65: 1027-1039.
  • Muluk ÇB, Kurt B, Turak A, Türker A, Çalışkan MA, Balkız Ö, Gümrükçü S, Sarıgül G, Zeydanlı U 2013. Türkiye’de Suyun Durumu ve Su Yönetiminde Yeni Yaklaşımlar: Çevresel Perspektif. İş Dünyası ve Sürdürülebilir Kalkınma Derneği - Doğa Koruma Merkezi.
  • Murashige T, Skoog F 1962. A Revised Medium for Rapid Growth and Bioassays With Tobacco Tissue Cultures. Plant Physiology, 15: 473-497.
  • Nath K, Saini S, Sharma YK 2005. Chromium in Tannery Industry Effluent and Its Effect on Plant Metabolism and Growth. Journal of Environmental Biology, 26: 197-204.
  • Piotrowska A, Bajguz A, Godlewska-Z yłkiewicz B, Czerpak R, Kaminska M 2009. Jasmonic Acid as Modulator of Lead Toxicity in Aquatic Plant Wolffia arrhiza (Lemnaceae). Environmental and Experimental Botany, 66: 507-513.
  • Satyakala G, Jamil Q 1992. Chromium Induced Biochemical Changes in Eichhornia crassipes (Mart.) Solms and Pistia stratioites. Bulletin of Environmental Contamination and Toxicology, 48: 921-928.
  • Sharma DC, Chatterjee C, Sharma CP 1995. Chromium Accumulation and Its Effect on Wheat (Triticum aestivum L. Cv. Dh2204) Metabolism. Plant Science, 111: 145-151.
  • Singh R, Tripathi RD, Dwivedi S, Kumar A, Trivedi PK, Chakrabarty D 2010. Lead Bioaccumulation Potential of an Aquatic Macrophyte Najas indica are Related to Antioxidant System. Bioresource Technology, 101: 3025-3032.
  • Tripathi RD, Rai RN, Gupta M, Yunus M, Chandra P 1995. Cadmium Transport in Submerged Macrophyte Ceratophyllum demersum L. in Presence of Various Metabolic Inhibitors and Calcium Channel Blockers. Chemosphere, 31: 3783-3791.
  • Yang X, Feng Y, He Z, Stoffella PJ 2005. Molecular Mechanisms of Heavy Metal Hyperaccumulation and Phytoremediation. Journal of Trace Elements in Medicine and Biology, 18: 339-353.
  • Yaowakhan P, Kruatrachue M, Pokethitiyook P, Soonthornsarathool V 2005. Removal of Lead Using Some Aquatic Macrophytes. Bulletin of Environmental Contamination and Toxicology, 75: 723-730.
  • Yousefi N, Meserghani M, Bahrami H, Mahvi AH 2016. Assessment of Human Health Risk for Heavy Metals in Imported Rice and Its Daily Intake in Iran. Research Journal of Environmental Toxicology, 10: 75-81.
  • Yüce M 2011. Bazı Ağır Metallerin Tayininde Kullanılmak Üzere Mikrobiyal Biyosensör Geliştirilmesi. A.Ü., Biyoteknoloji Ens., Doktora Tezi, 113 s.
  • Zayed A, Gowthaman S, Terry N 1998. Phytoremediation of Trace Elements by Wetland Plants: I. Duckweed. Journal of Environmental Quality, 27: 715-721.
  • Zhang FQ, Wang YS, Lou ZP, Dong JD 2007. Effect of Heavy Metal Stres on Antioxidative Enzymes and Lipid Peroxidation in Leaves and Roots of Two Mangrove Plant Seedlings (Kandelia candel and Bruguiera gymnorrhiz). Chemosphere, 67: 44-50.

In vitro Koşullarda Ceratophyllum demersum L.’un Krom (III) Akümülasyonunun Araştırılması

Year 2018, Volume: 21 Issue: 3, 277 - 285, 30.05.2018

Muhammet Doğan , Mehmet Karataş Muhammad Aasım

https://doi.org/10.18016/ksudobil.332980

Abstract

Bu çalışma,
farklı konsantrasyon (0-12 mg/L) ve sürelerde (1, 3 ve 5 gün) kroma (Cr) maruz
bırakılan Ceratophyllum dermersum L.
'un ağır metal akümülasyon seviyelerini ve Cr toksisitesinin taze ve kuru
ağırlıklar, fotosentetik pigmentasyon (klorofil a, klorofil b, toplam
klorofil ve karotenoid), protein içeriği ve lipid peroksidasyonu üzerine
etkilerini sunmaktadır. Cr akümülasyonu, metal konsantrasyonu ve uygulama
süresinin artışı ile artış göstermiştir. En fazla Cr akümülasyon değeri 12 mg/L
Cr uygulamasında ve 5 gün sonunda tespit edilmiştir. Cr toksisitesinden
kaynaklı fotosenteik pigment ve protein içeriklerinde önemli azalışlar
kaydedilmiştir. Kontrol gruplarıyla karşılaştırıldığında, minimum fotosentetik
pigment seviyeleri 5 gün sonra 12 mg/L kromda belirlenmiştir. Protein içeriğindeki
maksimum azalma %35.84 olarak tespit edilmiştir. Malondialdehit (MDA) içeriği,
lipid peroksidasyonunun bir ürünü olarak ölçülmüştür. Konsantrasyon ve uygulama
süresindeki artış ile doğru orantılı olarak MDA içeriği de artmıştır (p<0,05). Bitkideki maksimum MDA
seviyesi 12 mg/L kromda 15,58 nmol/g olarak ölçülmüştür.

Keywords

Ağır metal Biyoakümülasyon Cr toksisitesi Fitoremediasyon

References

  • Akın M, Akın G 2017. Suyun Önemi, Türkiye’de Su Potansiyeli,Su Havzaları ve Su Kirliliği. Ankara Üniversitesi Dil ve Tarih-Coğrafya Fakültesi Dergisi, 47: 105-118.
  • Ali H, Khan E, Sajad MA 2013. Phytoremediation of Heavy Metals-Concepts and Applications. Chemosphere, 91: 869-881.
  • Al-Ubaidy HJ, Rasheed KA 2015. Phytoremediation of Cadmium in River Water by Ceratophyllum demersum. World Journal Of Experimental Biosciences, 3: 14-17.
  • Arora M, Kiran B, Rani S, Rani A, Kaur B, Mittal N 2008. Heavy Metal Accumulation in Vegetables Irrigated with Water from Different Sources. Food Chemistry, 111: 811-815.
  • Bunluesin S, Kruatrachue M, Pokethitiyook P, Lanza GR, Upatham ES, Soonthornsarathool V 2004. Plant Screening and Comparison of Ceratophyllum demersum and Hydrilla verticillata for Cadmium Accumulation. Bulletin of Environmental Contamination and Toxicology, 73: 591-598.
  • Chen M, Zhang L, Li J, He X, Cai J 2015. Bioaccumulation and Tolerance Characteristics of a Submerged Plant (Ceratophyllum demersum L.) Exposed to Toxic Metal Lead. Ecotoxicology and Environmental Safety, 122: 313-321.
  • Costa M, Klein KB 2006. Toxicity and Carcinogenicity of Chromium Compounds in Humans. Critical Reviews in Toxicology, 36: 155-163.
  • Das S, Goswami S, Talukdar AD 2016. Physiological Responses of Water Hyacinth, Eichhornia crassipes (Mart.) Solms, to Cadmium and Its Phytoremediation Potential. Turkish Journal of Biology, 40: 84-94.
  • Doğan M 2005. Ceratophyllum demersum L.’de Kadmiyum Klorür, Sodyum Klorür ve Bunların Kombinasyonlarının Fizyolojik ve Morfolojik Etkileri. Ç.Ü. Fen Bil. Ens., Biyoloji ABD, Doktora Tezi, 137 s.
  • Duman F, Koca FD 2014. Single and Combined Effects of Exposure Concentration and Duration on Biological Responses of Ceratophyllum demersum L. Exposed to Cr Species. International Journal of Phytoremediation, 16: 1192-1208.
  • Duman F, Şahan S, Ceylan A, Koca FD 2010. Biological Responses of Ceratophyllum demersum L. Exposed to Chromium (Cr+6). SDU Journal of Science (E-Journal), 5: 163-171.
  • Eryılmaz H, İpek Şİ, Çelik BY 2014. Investigation of Water Quality of Borçka Dam Lake (Artvin). Journal of Science and Technology of Dumlupinar University, 33: 1-8.
  • Favas PJC, Pratas J, Varun M, D’Souza R, Paul MS 2014. Phytoremediation of Soils Contaminated with Metals and Metalloids at Mining Areas: Potential of Native Flora.( Envormental Risk Assessment of Soil Contamination, InTech Press: Ed. Hernandez-Soriano MC) 485-517.
  • Gaur JP, Noraho N, Chauhan YS 1994. Relationship Between Heavy Metal Accumulation and Toxicity in Spirodela polyrrhiza L. Schleid. and Azolla pinnata R. Br.. Aquatic Botany, 49: 183-192.
  • Gill RA, Zang L, Ali B, Farooq MA, Cui P, Yang Su, Ali S, Zhou W 2015. Chromium-Induced Physio-Chemical and Ultrastructural Changes in Four Cultivars of Brassica napus L.. Chemosphere, 120: 154-164.
  • Heath RL, Packer L 1968. Photoperoxidation in Isolated Chloroplasts I. Kinetic and Stoichiometry of Fatty Acid Peroxidation. Archives of Biochemistry and Biophysics, 125: 189-198.
  • Hoagland DR, Arnon DI 1950. The Water-Culture Method for Growing Plants without Soil. California Agricultural Experiment Station, Circular, 347: 1-32.
  • Kara Y, Zeytunluoglu A 2007. Bioaccumulation of Toxic Metals (Cd and Cu) by Groenlandia densa (L.) Fourr.. Bulletin of Environmental Contamination and Toxicology, 79: 609-612.
  • Koca FD 2012. Cr(III) ve Cr(VI) Maruziyetinde Ceratophyllum demersum L.’nin Biyolojik Cevabı: Konsantrasyon ve Uygulama Periyodunun Etkisi. E.Ü., Fen Bil. Ens., Biyoloji ABD, Yüksek Lisans Tezi, 75 s.
  • Lichtenthaler HK, Wellburn AR 1983. Determinations of Total Carotenoids and Chlorophylls a and b of Leaf Extracts in Different Solvents. Biochemical Society Transactions, 11: 591-592.
  • Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ, 1951. Protein Measurement with Folin Phenol Reagent. Journal of Biological Chemistry, 193: 265-275.
  • Memon AR, Schröder P 2009. Implications of Metal Accumulation Mechanisms to Phytoremediation. Environmental Science and Pollution Research, 16: 162-175.
  • Mishra S, Srivastava S, Tripathi RD, Kumar R, Seth CD, Gupta DK 2006. Lead Detoxification by Coontail (Ceratophyllum demersum L.) Involves Induction of hytochelatins and Antioxidant System in Response to Its Accumulation. Chemosphere, 65: 1027-1039.
  • Muluk ÇB, Kurt B, Turak A, Türker A, Çalışkan MA, Balkız Ö, Gümrükçü S, Sarıgül G, Zeydanlı U 2013. Türkiye’de Suyun Durumu ve Su Yönetiminde Yeni Yaklaşımlar: Çevresel Perspektif. İş Dünyası ve Sürdürülebilir Kalkınma Derneği - Doğa Koruma Merkezi.
  • Murashige T, Skoog F 1962. A Revised Medium for Rapid Growth and Bioassays With Tobacco Tissue Cultures. Plant Physiology, 15: 473-497.
  • Nath K, Saini S, Sharma YK 2005. Chromium in Tannery Industry Effluent and Its Effect on Plant Metabolism and Growth. Journal of Environmental Biology, 26: 197-204.
  • Piotrowska A, Bajguz A, Godlewska-Z yłkiewicz B, Czerpak R, Kaminska M 2009. Jasmonic Acid as Modulator of Lead Toxicity in Aquatic Plant Wolffia arrhiza (Lemnaceae). Environmental and Experimental Botany, 66: 507-513.
  • Satyakala G, Jamil Q 1992. Chromium Induced Biochemical Changes in Eichhornia crassipes (Mart.) Solms and Pistia stratioites. Bulletin of Environmental Contamination and Toxicology, 48: 921-928.
  • Sharma DC, Chatterjee C, Sharma CP 1995. Chromium Accumulation and Its Effect on Wheat (Triticum aestivum L. Cv. Dh2204) Metabolism. Plant Science, 111: 145-151.
  • Singh R, Tripathi RD, Dwivedi S, Kumar A, Trivedi PK, Chakrabarty D 2010. Lead Bioaccumulation Potential of an Aquatic Macrophyte Najas indica are Related to Antioxidant System. Bioresource Technology, 101: 3025-3032.
  • Tripathi RD, Rai RN, Gupta M, Yunus M, Chandra P 1995. Cadmium Transport in Submerged Macrophyte Ceratophyllum demersum L. in Presence of Various Metabolic Inhibitors and Calcium Channel Blockers. Chemosphere, 31: 3783-3791.
  • Yang X, Feng Y, He Z, Stoffella PJ 2005. Molecular Mechanisms of Heavy Metal Hyperaccumulation and Phytoremediation. Journal of Trace Elements in Medicine and Biology, 18: 339-353.
  • Yaowakhan P, Kruatrachue M, Pokethitiyook P, Soonthornsarathool V 2005. Removal of Lead Using Some Aquatic Macrophytes. Bulletin of Environmental Contamination and Toxicology, 75: 723-730.
  • Yousefi N, Meserghani M, Bahrami H, Mahvi AH 2016. Assessment of Human Health Risk for Heavy Metals in Imported Rice and Its Daily Intake in Iran. Research Journal of Environmental Toxicology, 10: 75-81.
  • Yüce M 2011. Bazı Ağır Metallerin Tayininde Kullanılmak Üzere Mikrobiyal Biyosensör Geliştirilmesi. A.Ü., Biyoteknoloji Ens., Doktora Tezi, 113 s.
  • Zayed A, Gowthaman S, Terry N 1998. Phytoremediation of Trace Elements by Wetland Plants: I. Duckweed. Journal of Environmental Quality, 27: 715-721.
  • Zhang FQ, Wang YS, Lou ZP, Dong JD 2007. Effect of Heavy Metal Stres on Antioxidative Enzymes and Lipid Peroxidation in Leaves and Roots of Two Mangrove Plant Seedlings (Kandelia candel and Bruguiera gymnorrhiz). Chemosphere, 67: 44-50.
There are 37 citations in total.

Details

Primary Language Turkish
Journal Section RESEARCH ARTICLE
Authors

Muhammet Doğan 0000-0003-3138-5903

Mehmet Karataş

Muhammad Aasım

Publication Date May 30, 2018
Submission Date August 6, 2017
Acceptance Date September 9, 2017
Published in Issue Year 2018 Volume: 21 Issue: 3

Cite

APA Doğan, M., Karataş, M., & Aasım, M. (2018). In vitro Koşullarda Ceratophyllum demersum L.’un Krom (III) Akümülasyonunun Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 21(3), 277-285. https://doi.org/10.18016/ksudobil.332980
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