Year 2019, Volume 22 , Issue 1, Pages 78 - 84 2019-02-28

Primula vulgaris Çiçek Ekstraktının Antioksidan Özellikleri ve İnsan Kanser Hücre Serileri Üzerindeki Sitotoksik Etkisi
Antioxidant Properties of Primula vulgaris Flower Extract and Its Cytotoxic Effect on Human Cancer Cell Lines

Selim DEMİR [1] , İbrahim TURAN [2] , Yüksel ALİYAZICIOĞLU [3]


Primula vulgaris, Primula cinsine mensup bir bitki olup, bu türler sıklıkla geleneksel tedavide kullanılmaktadırlar. Farklı Primula türlerinin antioksidan ve sitotoksik etkilerini konu alan çok sayıda çalışma olmasına rağmen, P. vulgaris ekstraktlarının bu özellikleri ile ilgili sınırlı sayıda çalışma bulunmaktadır. Bu çalışmanın amacı P. vulgaris çiçeklerinden dimetil sülfoksit ile hazırlanan ekstraktın antioksidan özelliklerinin ve sitotoksik etkilerinin belirlenmesidir. Ekstraktın toplam fenolik madde miktarı, toplam flavonoid madde miktarı ve indirgeyici antioksidan güç tayini spektrofotometrik yöntemler kullanılarak belirlendi. Ekstraktın sitotoksik etkisi ise insan kolon (WiDr), akciğer (A549), karaciğer (HepG2), meme (MCF-7), ve prostat (PC-3) kanser hücre serileri ile normal insan fibroblast hücre serisi üzerinde MTT yöntemi kullanılarak değerlendirildi. Ekstraktın toplam fenolik madde içeriği ve indirgeyici antioksidan güç̧ değerleri g örnek başına sırasıyla 33.02±0.92 mg gallik asit eşdeğeri ve 64.86±1.18 mg troloks eşdeğeri olarak bulundu. Ekstraktın çalışılan tüm kanser hücre serileri üzerinde normal fibroblast hücrelerine göre seçici sitotoksik etki gösterdiği belirlendi ve IC50 değerleri 191.8-375.3 μg/mL arasında hesaplandı. Bu çalışma P. vulgaris çiçek ekstraktının kuvvetli antioksidan özelliklerini ve kanser cre serileri üzerindeki seçici sitotoksik etkisini ortaya koyan öncü çalışmalardan birisidir. Ekstrakttaki etken molekülleri ve bu moleküllerin sitotoksik etki mekanizmalarını belirleyebilmek için daha ileri çalışmalara ihtiyaç duyulmaktadır.

Primula vulgaris is a plant belonging to Primula genus, and these species are often used in traditional therapy. Although there are numerous studies on the antioxidant and cytotoxic effects of different Primula species, there are limited studies of these properties of P. vulgaris extracts. The aim of this study was to determine the antioxidant properties and cytotoxic effects of the extract prepared with dimethyl sulfoxide from P. vulgaris flowers. The total phenolic content, total flavonoid content, and reducing antioxidant power of the extract were determined using spectrophotometric methods. The cytotoxic effect of the extract on human colon (WiDr), lung (A549), liver (HepG2), breast (MCF-7), and prostate (PC-3) cancer cells and a normal human fibroblast cell line was assessed using the MTT assay. Total phenolic content and reducing power values of extract were found 33.02±0.92 mg gallic acid equivalents, and 64.86±1.18 mg trolox equivalents per g sample, respectively. Extract exhibited selective cytotoxic effect on all studied cancer cells compared to normal fibroblast cells, and the IC50 values of the extract in the cancer cell lines range from 191.8 to 375.3 μg/mL. This work is one of the pioneering work that reveals the powerful antioxidant properties of P. vulgaris flower extract and its selective cytotoxic effect on cancer cell lines. Further studies are needed to identify the active molecules in the extract and the cytotoxic action mechanisms of these molecules.

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Primary Language en
Subjects Science
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0002-1863-6280
Author: Selim DEMİR (Primary Author)
Institution: Karadeniz Technical University
Country: Turkey


Orcid: 0000-0003-3400-5494
Author: İbrahim TURAN
Institution: GÜMÜŞHANE MÜHENDİSLİK FAKÜLTESİ
Country: Turkey


Orcid: 0000-0001-9474-4307
Author: Yüksel ALİYAZICIOĞLU
Institution: KARADENİZ TEKNİK ÜNİVERSİTESİ, TIP FAKÜLTESİ
Country: Turkey


Dates

Publication Date : February 28, 2019

APA DEMİR, S , TURAN, İ , ALİYAZICIOĞLU, Y . (2019). Antioxidant Properties of Primula vulgaris Flower Extract and Its Cytotoxic Effect on Human Cancer Cell Lines. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 22 (1) , 78-84 . DOI: 10.18016/ksutarimdoga.vi.460242