Year 2019, Volume 22 , Issue 5, Pages 763 - 771 2019-10-31

Türkiye’de Yetiştirilen Holştayn Irkı Sığırlarda GHRH ve PRL Gen Polimorfizmleri ile Süt Verimi Arasındaki İlişkinin Araştırılması
Investigation of the Relationship between GHRH and PRL Genes Polymorphsims and Milk Yield in Holstein Cattle Breed Reared in Turkey

Murat AKKAYA [1] , Bilal AKYÜZ [2]


Bu çalışmada, Türkiye’de yetiştirilen Holştayn ırkı sütçü sığırlarda büyüme hormonu salgılatıcı hormon (GHRH) ve prolaktin hormonu (PRL) genleri ile 305 günlük süt verimleri arasındaki ilişkinin araştırılması amaçlanmıştır. Çalışmada 150 baş sağmal Holştayn inek kullanılmıştır. GHRH genotiplerinin belirlenmesinde amacıyla yapılan PCR işlemi sonunda elde edilen PCR ürünleri HaeIII enzimiyle, PRL genotiplerinin belirlenmesinde amacıyla yapılan PCR işlemi sonunda elde edilen PCR ürünleri ise RsaI enzimi ile kesilmiştir. GHRH ve PRL genotipleri ile günlük ve laktasyon süt verimleri arasındaki ilişki varyans analizi ile değerlendirilmiştir. GHRH-HaeIII polimorfizmi yönünden incelenen örneklerinde iki allel (A ve B) ile üç genotip (AA, AB ve BB) gözlenmiş, B allel frekansının (0.68), A allel frekansından (0.32) yüksek olduğu görülmüştür. PRL-RsaI polimorfizmi yönünden incelenen ineklerde iki allel (A ve B) ile üç genotip (AA, AB ve BB) gözlenmiştir. A allel frekansı 0.86, B allel frekansı ise 0.14 olarak bulunmuştur. Çalışma sonunda incelenen Holştayn ineklerde GHRH-HaeIII ve PRL-RsaI polimorfizmleri ile 305 günlük süt verimleri arasında istatistiksel olarak önemli ilişki olmadığı belirlenmiştir. 

The aim of this study was to investigate the relationship between growth hormone, releasing hormone (GHRH) and prolactin hormone (PRL) genes polymorphisms and 305-day milk yield in Hostein dairy cattle reared in Turkey. Overall, 150 Hostein dairy cows was used for the study. In order to determine GHRH genotypes, obtained PCR products were restricted via HaeIII enzyme and to determine PRL genotypes, obtained PCR products were restricted via RsaI enzyme. The mean differences of 305-day milk yield among the genotype groups of GHRH and PRL genes were assessed by analysis of variance (ANOVA). Two alleles (A and B) and three genotypes (AA, AB and BB) for GHRH-HaeIII polymorphism were observed in examined Hostein cows. The frequency of the A and B alleles was found to be 0.32 and 0.68, respectively in examined Hostein cows. Two alleles (A and B) and three genotypes (AA, AB and BB) for PRL-RsaI polymorphism were observed. The frequency of the A and B alleles was found to be 0.86 and 0.14, respectively. Finally, GHRH-HaeIII and PRL-RsaI genotype effects on daily and lactation milk yield was not found to be significant in examined animals. 

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Primary Language tr
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0001-8354-6516
Author: Murat AKKAYA
Institution: SAĞLIK BİLİMLERİ ENSTİTÜSÜ

Orcid: 0000-0001-7548-9830
Author: Bilal AKYÜZ (Primary Author)
Institution: ERCİYES ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ
Country: Turkey


Thanks Bu çalışmayı TYL-2017-7703 proje kodlu destekleyen Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi’ne teşekkür ederiz.
Dates

Publication Date : October 31, 2019

APA AKKAYA, M , AKYÜZ, B . (2019). Türkiye’de Yetiştirilen Holştayn Irkı Sığırlarda GHRH ve PRL Gen Polimorfizmleri ile Süt Verimi Arasındaki İlişkinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 22 (5) , 763-771 . DOI: 10.18016/ksutarimdoga.vi.530786