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Research Article
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Investigation of the Relationship between GHRH and PRL Genes Polymorphsims and Milk Yield in Holstein Cattle Breed Reared in Turkey

Year 2019, Volume: 22 Issue: 5, 763 - 771, 31.10.2019

Murat Akkaya Bilal Akyüz

https://doi.org/10.18016/ksutarimdoga.vi.530786

Abstract

The aim of this study was to investigate the
relationship between growth hormone, releasing hormone (GHRH) and prolactin hormone (PRL)
genes polymorphisms and 305-day milk yield in Hostein dairy cattle reared in
Turkey. Overall, 150 Hostein dairy cows was used for the study. In order to
determine GHRH genotypes, obtained
PCR products were restricted via HaeIII
enzyme and to determine PRL genotypes,
obtained PCR products were restricted via RsaI
enzyme. The mean differences of 305-day milk yield among the genotype groups of
GHRH and PRL genes were assessed by analysis of variance (ANOVA). Two alleles
(A and B) and three genotypes (AA, AB and BB) for GHRH-HaeIII polymorphism were
observed in examined Hostein cows. The frequency of the A and B alleles was
found to be 0.32 and 0.68, respectively in examined Hostein cows. Two alleles
(A and B) and three genotypes (AA, AB and BB) for PRL-RsaI polymorphism were
observed. The frequency of the A and B alleles was found to be 0.86 and 0.14,
respectively. Finally, GHRH-HaeIII and PRL-RsaI genotype effects on daily and
lactation milk yield was not found to be significant in examined animals. 

Keywords

Hostein Milk yield GHRH PRL RFLP

References

  • Akyuz B, Agaoglu OK, Ertugrul O 2012. Genetic polymorphism of kappa‐casein, growth hormone and prolactin genes in Turkish native cattle breeds. International Journal of Dairy Technology, 65(1): 38-44.
  • Akyüz B, Arslan K, Bayram D, İşcan KM 2013. Allelic frequency of kappa-casein, growth hormone and prolactin gene in Holstein, Brown Swiss and Simmental cattle breeds in Turkey. Kafkas Universitesi Veteriner Fakultesi Dergisi, 19(3): 439-444.
  • Alipanah M, Kalashnikova L, Rodionov G 2007. Association of prolactin gene variants with milk production traits in Russian Red Pied cattle. Iranian Journal of Biotechnology, 5(3): 158-161.
  • Auchtung TL, Buchanan DS, Lents CA, Barao SM, Dahl GE 2001. Growth hormone response to growth hormone-releasing hormone in beef cows divergently selected for milk production. Journal of Dairy Science, 79(5): 1295-1300.
  • Baile CA, Buonomo FC 1987. Growth hormone-releasing factor effects on pituitary-function, growth, and lactation. Journal of Dairy Science, 70(2): 467-473.
  • Bonneau M, Laarveld B 1999. Biotechnology in animal nutrition, physiology and health. Livestock Production Science, 59(2-3): 223-241.
  • Cemal İ, Karaca O 2006. Çiftlik hayvanlarında major genlerin belirlenmesi ve genotip ayrımı. Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Dergisi, 21(1): 105-115.
  • Chrenek P, Huba J, Oravcova M, Hetenyi L, Peskovieova D, Bulla J 1999. Genotypes of bGH and bPRL genes in relationships to milk production. EAAP 50th Annual Meeting, 22-26 Augst, Zurich.
  • Curi RA, De Oliveira HN, Silveira AC, Lopes CR 2005. Association between IGF-I, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livestock Production Science, 94(3): 159-167.
  • Czerniawska-Piątkowska E, Szewczuk M, Zych S 2011. Association between genetic polymorphism of growth-hormone-releasing hormone and the yield, chemical composition and technological parameters of cow milk (brief report). Archives Animal Breeding, 54(3): 323-325.
  • Dahl GE, Chapin LT, Moseley WM, Tucker HA 1993. Galactopoietic effects of recombinant somatotropin and growth hormone- releasing factor in dairy cows. Journal of Dairy Science, 76(6): 1550-1557.
  • Dybus A, Kmiec M, Sobek Z,Pietrzyk W, Wisniewski B 2003. Associations between polymorphisms of growth hormone releasing hormone (GHRH) and pituitary transcription factor 1 (PIT1) genes and production traits of limousine cattle. Archives Animal Breeding, 46(6): 527-534.
  • Dybus A, Grzesiak W, Kamieniecki H, Szatkowska I, Sobek Z, Blaszczyk P, Czerniawska-Piatrowska E, Zych S, Muszynska M 2005. Association of genetic variants of bovine prolactin with milk production traits of Black-and-White and Jersey cattle. Archives Animal Breeding, 48: 149-156.
  • Dybus A, Grzesiak W 2006. GHRH/HaeIII gene polymorphism and its associations with milk production traits in Polish Black-and-White cattle. Archives Animal Breeding, 49(5): 434-438.
  • Erhardt G, Weimann C (2007). Use of molecular markers for evaluation of genetic diversity and in animal production. Archivos Latinoamericanos de Producción Animal, 15(supl 1): 63-66.
  • Frohman LA, Downs TR, Chomczynski P 1992. Regulation of growth hormone secretion. Frontiers in Neuroendocrinology, 13(4): 344-405.
  • Hu X, Lü A, Chen H, Gao X, Xu H, Zhang C 2009. Preliminary evidence for association of prolactin and prolactin receptor genes with milk production traits in Chinese Holsteins. Journal of Applied Animal Research, 36(2): 213-217.
  • Jiang L, Liu J, Sun D, Ma P, Ding X, Yu Y, Zhang Q 2010. Genome wide association studies for milk production traits in Chinese Holstein population. Plos ONE, 5(10): e13661.
  • Kaygısız A,Yılmaz İ, Koşum S 2017. Şanlıurfa ilinde Siyah Alaca ırkı sığırların yetiştirici şartlarında bazı adaptasyon özellikleri. KSÜ Doğa Bilimleri Dergisi, 20(2): 133-136.
  • Khatami SR, Lazebny OE, Maksimenko VF, Sulimova GE 2005. Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and black-and-white cattle. Russian Journal of Genetics, 41(2): 167–173.
  • Kmiec M, Kowalewska-Luczak I, Kulig H, Terman A, Wierzbicki H, Lepczynki A 2007. Associations Between GHRH/Haelll restriction polymorphism and milk production traits in a herd of dairy cattle. Journal of Animal and Veterinary Advances, 6(11): 1298-1303.
  • Konca MA, Akyüz B 2017. Investigation of growth hormone releasing hormone, growth hormone and prolactin hormone gene polymorphism in Anatolian water buffalo. Annals of Animal Science, 17(4): 1053-1062.
  • Lacasse P, Ollier S 2015. The dopamine antagonist domperidone increases prolactin concentration and enhances milk production in dairy cows. Journal of Dairy Science, 98(11): 7856-7864.
  • Løvendahl P, Woolliams JA, Sinnett-Smith PA 1991. Response of growth hormone to various doses of growth hormone releasing factor and thyrotropin releasing hormone administered separately and in combination to dairy calves. Canadian Journal of Animal Science, 71: 1045-1052.
  • Mauriæ M, Mašek T, Beniæ M, Špehar M, Starèeviæ 2017. Effect of DGAT1, FASN and PRL genes on milk production and milk composition traits in Simmental and crossbred Holstein cattle. Indian Journal of Animal Sciences, 87(7): 859-863.
  • Mehmannavaz Y, Amirinia C, Bonyadi M, Tordhizi RV 2009. Effects of bovine prolactin gene polymorphism within exon 4 on milk related traits and genetic trends in Iranian Holstein bulls. African Journal of Biotechnology, 8(19): 4797-4801.
  • Miltiadou D, Orford M, Symeou S, Banos G 2017. Identification of variation in the ovine prolactin gene of Chios sheep with a cost-effective sequence-based typing assay. Journal of Dairy Science, 100(2): 1290-1294.
  • Othman OE, Abdel-Samad MF, El-Maaty NAA, Sewifty KM 2015. Genotyping and nucleotide sequences of growth hormone releasing hormone and its receptor genes in Egyptian buffalo. British Biotechnology Journal, 5(2): 62-71.
  • Parmentier I, Portetellea D, Gengler N, Prandi A, Bertozzi C, Vleurick L, Gilson R, Renaville R (1999). Candidate gene markers associated with somatotropic axis and milk selection. Domestic Animal Endocrinology, 17: 139-148.
  • Patel, JB, Chauhan JB 2017. Polymorphism of the prolactin gene and its relationship with milk production in Gir and Kankrej cattle. Journal of Natural Science, Biology and Medicine, 8(2): 167-170.
  • Rini AO, Sumantri C, Anggraeni A 2013. GHRH/HaeIII polymorphism in dairy and beef cattle at natioan livestock breeding centers. Media Peternakan, 36(3): 185-191.
  • Sambrook J, Fritsch EF, Maniatis T 1989. Molecular Clonning: A Laboratory Manual. Second Edition, Cold-Spring Harbor, New York, USA, Volume 2, pp: 9.16-9.19.
  • Singh U, Deb R, Alyethodi RR, Alex R, Kumar S, Chakraborty S, Dhama K, Sharma A 2014. Molecular markers and their applications in cattle genetic research: A review. Biomarkers and Genomic Medicine, 6(2): 49-58.
  • Sodhi M, Mukesh M, Mishra BP, Parvesh K, Joshi BK 2011. Analysis of genetic variation at the prolactin-RsaI (PRL-RsaI) locus in Indian native cattle breeds (Bos indicus). Biochemical Genetics, 49(1-2): 39-45.
  • Szewczuk M, Zych S, Chaberski R 2008. Effect of growth hormone-releasing hormone gene polymorphism (GHRH/HaeIII) on milk performance in Polish Holstein-Friesian cows. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, Sbornik Mendelovy Zemedelske A Lesnicke Univerzty V Brne, 56(4): 177-182.
  • Szatkowska I, Dybus A, Grzesiak W, Jedrzejczak M, Muszynska M 2009. Association between the growth hormone releasing hormone (GHRH) gene polymorphism and milk production traits of dairy cattle. Journal of Applied Animal Research, 36(1): 119-123.
  • Thuy NTD, Thu NT, Cuong NH, Ty LV, Nguyen TTB, Khoa DVA 2018. Polymorphism of PIT-1 and prolactin genes and their effects on milk yield in Holştayn Frisian dairy cows bred in Vietnam. Russian Journal of Genetics, 54(3): 346-352.
  • Tyul’kin SV, Akhmetov TM, Valiullina EF, Vafin RR 2013. Polymorphism of somatotropin, prolactin, leptin, and thyreoglobulin genes in bulls. Russian Journal of Genetics: Applied Research, 3(3): 222-224.
  • Yıldırım F, Özdemir S, Yıldız A 2018. Koçaş tarım işletmesinde yetiştirilen Siyah Alaca (Holştayn) sığırlarda bazı süt verimi özellikleri ve ilişkili genlerin ekspresyonu. KSÜ Tarım ve Doğa Dergisi, 21(3): 353-362.

Türkiye’de Yetiştirilen Holştayn Irkı Sığırlarda GHRH ve PRL Gen Polimorfizmleri ile Süt Verimi Arasındaki İlişkinin Araştırılması

Year 2019, Volume: 22 Issue: 5, 763 - 771, 31.10.2019

Murat Akkaya Bilal Akyüz

https://doi.org/10.18016/ksutarimdoga.vi.530786

Abstract

Bu çalışmada, Türkiye’de yetiştirilen Holştayn ırkı sütçü sığırlarda
büyüme hormonu salgılatıcı hormon (GHRH)
ve prolaktin hormonu (PRL) genleri
ile 305 günlük süt verimleri arasındaki ilişkinin araştırılması amaçlanmıştır.
Çalışmada 150 baş sağmal Holştayn inek kullanılmıştır. GHRH genotiplerinin belirlenmesinde amacıyla yapılan PCR işlemi
sonunda elde edilen PCR ürünleri HaeIII enzimiyle, PRL genotiplerinin belirlenmesinde amacıyla yapılan PCR işlemi
sonunda elde edilen PCR ürünleri ise RsaI enzimi ile kesilmiştir. GHRH ve PRL genotipleri ile günlük
ve laktasyon süt verimleri arasındaki
ilişki varyans analizi ile değerlendirilmiştir. GHRH-HaeIII polimorfizmi yönünden incelenen örneklerinde iki allel
(A ve B) ile üç genotip (AA, AB ve BB) gözlenmiş, B allel frekansının (0.68), A
allel frekansından (0.32) yüksek olduğu görülmüştür. PRL-RsaI polimorfizmi yönünden incelenen ineklerde iki allel (A ve
B) ile üç genotip (AA, AB ve BB) gözlenmiştir. A allel frekansı 0.86, B allel
frekansı ise 0.14 olarak bulunmuştur. Çalışma sonunda incelenen Holştayn
ineklerde GHRH-HaeIII ve PRL-RsaI polimorfizmleri ile 305 günlük
süt verimleri arasında istatistiksel olarak önemli ilişki olmadığı
belirlenmiştir. 

Keywords

Holştayn GHRH PRL RFLP Süt verimi

Thanks

Bu çalışmayı TYL-2017-7703 proje kodlu destekleyen Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi’ne teşekkür ederiz.

References

  • Akyuz B, Agaoglu OK, Ertugrul O 2012. Genetic polymorphism of kappa‐casein, growth hormone and prolactin genes in Turkish native cattle breeds. International Journal of Dairy Technology, 65(1): 38-44.
  • Akyüz B, Arslan K, Bayram D, İşcan KM 2013. Allelic frequency of kappa-casein, growth hormone and prolactin gene in Holstein, Brown Swiss and Simmental cattle breeds in Turkey. Kafkas Universitesi Veteriner Fakultesi Dergisi, 19(3): 439-444.
  • Alipanah M, Kalashnikova L, Rodionov G 2007. Association of prolactin gene variants with milk production traits in Russian Red Pied cattle. Iranian Journal of Biotechnology, 5(3): 158-161.
  • Auchtung TL, Buchanan DS, Lents CA, Barao SM, Dahl GE 2001. Growth hormone response to growth hormone-releasing hormone in beef cows divergently selected for milk production. Journal of Dairy Science, 79(5): 1295-1300.
  • Baile CA, Buonomo FC 1987. Growth hormone-releasing factor effects on pituitary-function, growth, and lactation. Journal of Dairy Science, 70(2): 467-473.
  • Bonneau M, Laarveld B 1999. Biotechnology in animal nutrition, physiology and health. Livestock Production Science, 59(2-3): 223-241.
  • Cemal İ, Karaca O 2006. Çiftlik hayvanlarında major genlerin belirlenmesi ve genotip ayrımı. Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Dergisi, 21(1): 105-115.
  • Chrenek P, Huba J, Oravcova M, Hetenyi L, Peskovieova D, Bulla J 1999. Genotypes of bGH and bPRL genes in relationships to milk production. EAAP 50th Annual Meeting, 22-26 Augst, Zurich.
  • Curi RA, De Oliveira HN, Silveira AC, Lopes CR 2005. Association between IGF-I, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livestock Production Science, 94(3): 159-167.
  • Czerniawska-Piątkowska E, Szewczuk M, Zych S 2011. Association between genetic polymorphism of growth-hormone-releasing hormone and the yield, chemical composition and technological parameters of cow milk (brief report). Archives Animal Breeding, 54(3): 323-325.
  • Dahl GE, Chapin LT, Moseley WM, Tucker HA 1993. Galactopoietic effects of recombinant somatotropin and growth hormone- releasing factor in dairy cows. Journal of Dairy Science, 76(6): 1550-1557.
  • Dybus A, Kmiec M, Sobek Z,Pietrzyk W, Wisniewski B 2003. Associations between polymorphisms of growth hormone releasing hormone (GHRH) and pituitary transcription factor 1 (PIT1) genes and production traits of limousine cattle. Archives Animal Breeding, 46(6): 527-534.
  • Dybus A, Grzesiak W, Kamieniecki H, Szatkowska I, Sobek Z, Blaszczyk P, Czerniawska-Piatrowska E, Zych S, Muszynska M 2005. Association of genetic variants of bovine prolactin with milk production traits of Black-and-White and Jersey cattle. Archives Animal Breeding, 48: 149-156.
  • Dybus A, Grzesiak W 2006. GHRH/HaeIII gene polymorphism and its associations with milk production traits in Polish Black-and-White cattle. Archives Animal Breeding, 49(5): 434-438.
  • Erhardt G, Weimann C (2007). Use of molecular markers for evaluation of genetic diversity and in animal production. Archivos Latinoamericanos de Producción Animal, 15(supl 1): 63-66.
  • Frohman LA, Downs TR, Chomczynski P 1992. Regulation of growth hormone secretion. Frontiers in Neuroendocrinology, 13(4): 344-405.
  • Hu X, Lü A, Chen H, Gao X, Xu H, Zhang C 2009. Preliminary evidence for association of prolactin and prolactin receptor genes with milk production traits in Chinese Holsteins. Journal of Applied Animal Research, 36(2): 213-217.
  • Jiang L, Liu J, Sun D, Ma P, Ding X, Yu Y, Zhang Q 2010. Genome wide association studies for milk production traits in Chinese Holstein population. Plos ONE, 5(10): e13661.
  • Kaygısız A,Yılmaz İ, Koşum S 2017. Şanlıurfa ilinde Siyah Alaca ırkı sığırların yetiştirici şartlarında bazı adaptasyon özellikleri. KSÜ Doğa Bilimleri Dergisi, 20(2): 133-136.
  • Khatami SR, Lazebny OE, Maksimenko VF, Sulimova GE 2005. Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and black-and-white cattle. Russian Journal of Genetics, 41(2): 167–173.
  • Kmiec M, Kowalewska-Luczak I, Kulig H, Terman A, Wierzbicki H, Lepczynki A 2007. Associations Between GHRH/Haelll restriction polymorphism and milk production traits in a herd of dairy cattle. Journal of Animal and Veterinary Advances, 6(11): 1298-1303.
  • Konca MA, Akyüz B 2017. Investigation of growth hormone releasing hormone, growth hormone and prolactin hormone gene polymorphism in Anatolian water buffalo. Annals of Animal Science, 17(4): 1053-1062.
  • Lacasse P, Ollier S 2015. The dopamine antagonist domperidone increases prolactin concentration and enhances milk production in dairy cows. Journal of Dairy Science, 98(11): 7856-7864.
  • Løvendahl P, Woolliams JA, Sinnett-Smith PA 1991. Response of growth hormone to various doses of growth hormone releasing factor and thyrotropin releasing hormone administered separately and in combination to dairy calves. Canadian Journal of Animal Science, 71: 1045-1052.
  • Mauriæ M, Mašek T, Beniæ M, Špehar M, Starèeviæ 2017. Effect of DGAT1, FASN and PRL genes on milk production and milk composition traits in Simmental and crossbred Holstein cattle. Indian Journal of Animal Sciences, 87(7): 859-863.
  • Mehmannavaz Y, Amirinia C, Bonyadi M, Tordhizi RV 2009. Effects of bovine prolactin gene polymorphism within exon 4 on milk related traits and genetic trends in Iranian Holstein bulls. African Journal of Biotechnology, 8(19): 4797-4801.
  • Miltiadou D, Orford M, Symeou S, Banos G 2017. Identification of variation in the ovine prolactin gene of Chios sheep with a cost-effective sequence-based typing assay. Journal of Dairy Science, 100(2): 1290-1294.
  • Othman OE, Abdel-Samad MF, El-Maaty NAA, Sewifty KM 2015. Genotyping and nucleotide sequences of growth hormone releasing hormone and its receptor genes in Egyptian buffalo. British Biotechnology Journal, 5(2): 62-71.
  • Parmentier I, Portetellea D, Gengler N, Prandi A, Bertozzi C, Vleurick L, Gilson R, Renaville R (1999). Candidate gene markers associated with somatotropic axis and milk selection. Domestic Animal Endocrinology, 17: 139-148.
  • Patel, JB, Chauhan JB 2017. Polymorphism of the prolactin gene and its relationship with milk production in Gir and Kankrej cattle. Journal of Natural Science, Biology and Medicine, 8(2): 167-170.
  • Rini AO, Sumantri C, Anggraeni A 2013. GHRH/HaeIII polymorphism in dairy and beef cattle at natioan livestock breeding centers. Media Peternakan, 36(3): 185-191.
  • Sambrook J, Fritsch EF, Maniatis T 1989. Molecular Clonning: A Laboratory Manual. Second Edition, Cold-Spring Harbor, New York, USA, Volume 2, pp: 9.16-9.19.
  • Singh U, Deb R, Alyethodi RR, Alex R, Kumar S, Chakraborty S, Dhama K, Sharma A 2014. Molecular markers and their applications in cattle genetic research: A review. Biomarkers and Genomic Medicine, 6(2): 49-58.
  • Sodhi M, Mukesh M, Mishra BP, Parvesh K, Joshi BK 2011. Analysis of genetic variation at the prolactin-RsaI (PRL-RsaI) locus in Indian native cattle breeds (Bos indicus). Biochemical Genetics, 49(1-2): 39-45.
  • Szewczuk M, Zych S, Chaberski R 2008. Effect of growth hormone-releasing hormone gene polymorphism (GHRH/HaeIII) on milk performance in Polish Holstein-Friesian cows. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, Sbornik Mendelovy Zemedelske A Lesnicke Univerzty V Brne, 56(4): 177-182.
  • Szatkowska I, Dybus A, Grzesiak W, Jedrzejczak M, Muszynska M 2009. Association between the growth hormone releasing hormone (GHRH) gene polymorphism and milk production traits of dairy cattle. Journal of Applied Animal Research, 36(1): 119-123.
  • Thuy NTD, Thu NT, Cuong NH, Ty LV, Nguyen TTB, Khoa DVA 2018. Polymorphism of PIT-1 and prolactin genes and their effects on milk yield in Holştayn Frisian dairy cows bred in Vietnam. Russian Journal of Genetics, 54(3): 346-352.
  • Tyul’kin SV, Akhmetov TM, Valiullina EF, Vafin RR 2013. Polymorphism of somatotropin, prolactin, leptin, and thyreoglobulin genes in bulls. Russian Journal of Genetics: Applied Research, 3(3): 222-224.
  • Yıldırım F, Özdemir S, Yıldız A 2018. Koçaş tarım işletmesinde yetiştirilen Siyah Alaca (Holştayn) sığırlarda bazı süt verimi özellikleri ve ilişkili genlerin ekspresyonu. KSÜ Tarım ve Doğa Dergisi, 21(3): 353-362.
There are 39 citations in total.

Details

Primary Language Turkish
Subjects Agricultural, Veterinary and Food Sciences
Journal Section RESEARCH ARTICLE
Authors

Murat Akkaya 0000-0001-8354-6516

Bilal Akyüz 0000-0001-7548-9830

Publication Date October 31, 2019
Submission Date February 22, 2019
Acceptance Date April 18, 2019
Published in Issue Year 2019 Volume: 22 Issue: 5

Cite

APA Akkaya, M., & Akyüz, B. (2019). Türkiye’de Yetiştirilen Holştayn Irkı Sığırlarda GHRH ve PRL Gen Polimorfizmleri ile Süt Verimi Arasındaki İlişkinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 22(5), 763-771. https://doi.org/10.18016/ksutarimdoga.vi.530786
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