Year 2019, Volume 22 , Issue 6, Pages 843 - 850 2019-12-31

Ağır Metal Stresinin Rokada (Eruca sativa L.) Bitki Büyümesi ve Fizyolojisi Üzerine Etkisi
Impact of Cadmium and Lead Heavy Metal Stress on Plant Growth and Physiology of Rocket (Eruca sativa L.)

Ertan YILDIRIM [1] , Melek EKİNCİ [2] , Metin TURAN [3] , Güleray AGAR [4] , Selda ÖRS [5] , Atilla DURSUN [6] , Raziye KUL [7] , Tayfun BALCI [8]


Bu çalışma, sera koşullarında kadmiyum (Cd) (0, 100, 150 ve 200 mg kg-1) ve kurşunun (Pb) (0, 1000, 1500 ve 2000 mg kg-1) rokada (Eruca sativa L.) morfolojik, fizyolojik ve biyokimyasal tepkiler üzerine etkilerini belirlemek amacıyla yapılmıştır. Roka bitkilerinin büyüme, bazı fizyolojik (membran geçirgenliği, nisbi su içeriği, stoma iletkenliği vb.) ve biyokimyasal (antioksidan enzim aktivitesi, prolin ve sukroz içeriği) parametreleri ağır metal stres koşulları altında değişmiştir. Rokanın Cd ve Pb içeriği yüksek konsantrasyonla artmıştır. Hem Cd hem de Pb stres koşulları bitki büyümesini, fotosentetik aktiviteyi ve klorofil içeriğini olumsuz etkilemiştir. Ağır metallerin negatif etkisi artan Cd ve Pb dozları ile daha fazla olmuştur. Cd ve Pb uygulamaları antioksidan enzim aktivitelerini önemli ölçüde artırmıştır. Ayrıca, ağır metal stresli bitkilerde, kontrol bitkilerine kıyasla daha fazla malondialdehit (MDA), hidrojen peroksit (H2O2), prolin ve sukroz bulunmuştur. Bu çalışma, roka bitkilerinin enzim aktivitesini ve osmolit birikimini düzenleyerek ağır metal stresine karşı savunma mekanizmaları geliştirdiklerini göstermiştir.

This study was conducted to evaluate the influence of cadmium (Cd) (0, 100, 150 and 200 mg kg-1) and lead (Pb) (0, 1000, 1500 and 2000 mg kg-1) on morphological, physiological and biochemical responses of rocket (Eruca sativa L.) in greenhouse conditions. Plant growth, some physiological (membrane permeability, relative water content, stomal conductance, etc.) and biochemical (antioxidant enzyme activity, proline and sucrose content) parameters of rocket plants were altered with Cd and Pb levels. The Cd and Pb content in rocket increased with elevated concentration. Both Cd and Pb stress conditions negatively affected plant growth, photosynthetic activity and chlorophyll content. The negative effect of heavy metals elevated with increased doses of Cd and Pb. The treatment of Cd and Pb significantly elevated antioxidant enzyme activities. Furthermore, the heavy metal stressed plants had more malondialdehyde (MDA), hydrogen peroxide (H2O2), proline and sucrose as compared to control plants. This study indicated that rocket plants developed defense mechanisms by regulating enzyme activity and osmolyte accumulation against heavy metal stress.

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Primary Language en
Subjects Agriculture
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0003-3369-0645
Author: Ertan YILDIRIM
Institution: ATATURK UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF HORTICULTURE
Country: Turkey


Orcid: 0000-0002-7604-3803
Author: Melek EKİNCİ (Primary Author)
Institution: ATATURK UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF HORTICULTURE
Country: Turkey


Orcid: 0000-0002-4849-7680
Author: Metin TURAN
Institution: YEDITEPE UNIVERSITY, FACULTY OF ENGINEERING AND ARCHITECTURE, DEPARTMENT OF GENETICS AND BIOENGINEERING
Country: Turkey


Orcid: 0000-0002-8445-5082
Author: Güleray AGAR
Institution: ATATURK UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY
Country: Turkey


Orcid: 0000-0001-6789-8642
Author: Selda ÖRS
Institution: ATATURK UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF AGRICULTURAL STRUCTURES AND IRRIGATION
Country: Turkey


Orcid: 0000-0002-8475-8534
Author: Atilla DURSUN
Institution: ATATURK UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF HORTICULTURE
Country: Turkey


Orcid: 0000-0002-5836-6473
Author: Raziye KUL
Institution: ATATURK UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF HORTICULTURE
Country: Turkey


Orcid: 0000-0002-8255-6760
Author: Tayfun BALCI
Institution: ATATURK UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF HORTICULTURE
Country: Turkey


Supporting Institution Atatürk University, Scientific Research Projects Foundation
Project Number Project Number FHD-2018-6702
Thanks We appreciate Atatürk University, Scientific Research Projects Foundation for generous financial support (Project Number FHD-2018-6702).
Dates

Publication Date : December 31, 2019

APA YILDIRIM, E , EKİNCİ, M , TURAN, M , AGAR, G , ÖRS, S , DURSUN, A , KUL, R , BALCI, T . (2019). Impact of Cadmium and Lead Heavy Metal Stress on Plant Growth and Physiology of Rocket (Eruca sativa L.). Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 22 (6) , 843-850 . DOI: 10.18016/ksutarimdoga.vi.548626