Year 2019, Volume 22 , Issue , Pages 361 - 364 2019-12-31

The Effects of Different Storage Conditions and Storage Time on The Quality of The Mitochondrial DNA on Insects: Sample Study is A Fruit Fly (Diptera: Tephiritidae)
Böceklerde Farklı Muhafaza Koşulları ve Zamanın Mitokondrial DNA Kalitesi Üzerine Etkisi: Bir Diptera (Tephiritidae) Örneği Çalışması

Adile AKPINAR [1] , Fidan JUMAID [2] , Vedat GÖRMEZ [3] , Murat KÜTÜK [4] , Canan CAN [5]


In this study, DNA was isolated from the samples of Euaresta bullans (Wiedemann, 1830) belonging to the Tephritidae family which was obtained by traditional killing methods and kept under different conditions. In this sense, the standard barcode gene mitochondrial cytochrome oxidase I gene (COI) was used. The lack of relationship observed between the age of samples that were kept in dry conditions (museum material) for 15 years and PCR success. However, the DNA quality and PCR success of the samples stored in alcohol (95% ethyl alcohol) were found to be high.

Bu çalışmada geleneksel öldürme yöntemleri ile elde edilmiş ve farklı koşullarda muhafaza edilen Tephritidae familyasına ait Euaresta bullans (Wiedemann, 1830) örneklerinden DNA izole edilerek muhafaza koşulları ve muhafaza sürelerine bağlı olarak DNA kalitesi ve PZR başarıları araştırılmıştır. Bu anlamda standart barkod geni olan mitokondriyal sitokrom oksidaz I  (COI) gen bölgesi kullanılmıştır. 15 yıllık örneklerden kuru ortamda (müze materyali) muhafaza edilenlerin yaşı ile PZR başarısı arasında tam bir ilişki gözlemlenmemiştir. Ancak alkolde (%95’lik etil alkol) saklanan örneklerde ise DNA kalitesi ve PZR başarısı yüksek bulunmuştur. 

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Primary Language tr
Subjects Biology
Journal Section RESEARCH ARTICLE
Authors

Orcid: 0000-0001-5815-1096
Author: Adile AKPINAR (Primary Author)
Institution: GAZİANTEP ÜNİVERSİTESİ, FEN EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Country: Turkey


Orcid: 0000-0001-8839-7695
Author: Fidan JUMAID
Institution: GAZİANTEP ÜNİVERSİTESİ, FEN EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Country: Turkey


Orcid: 0000-0001-8136-8226
Author: Vedat GÖRMEZ
Institution: GAZİANTEP ÜNİVERSİTESİ, FEN EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Country: Turkey


Orcid: 0000-0003-1567-1002
Author: Murat KÜTÜK
Institution: GAZİANTEP ÜNİVERSİTESİ, FEN EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Country: Turkey


Orcid: 0000-0002-0473-1914
Author: Canan CAN
Institution: GAZİANTEP ÜNİVERSİTESİ, FEN EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ
Country: Turkey


Supporting Institution Gaziantep Üniversitesi BAP Yönetim Birimi
Project Number FEF.YLT.18.20
Thanks Bu çalışmaya, Gaziantep Üniversitesi, Bilimsel Araştırma Projeleri Biriminin, FEF.YLT.18.20 no’lu proje ile vermiş oldukları destekten dolayı teşekkür ederiz.
Dates

Application Date : May 24, 2019
Acceptance Date : August 23, 2019
Publication Date : December 31, 2019

APA AKPINAR, A , JUMAID, F , GÖRMEZ, V , KÜTÜK, M , CAN, C . (2019). Böceklerde Farklı Muhafaza Koşulları ve Zamanın Mitokondrial DNA Kalitesi Üzerine Etkisi: Bir Diptera (Tephiritidae) Örneği Çalışması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi , 22 () , 361-364 . DOI: 10.18016/ksutarimdoga.vi.569813