Yeni Laktik Asit Bakteri İzolatlarının Farklı Kuru Madde İçeriğine Sahip Yonca (Medicago sativa L.) Silajında Mikrobiyel İnokulant Olarak Kullanılma Olanakları

Mustafa Kızılsımsek; Kübra Keklik; Tuğba Günaydın

doi:10.18016/ksutarimdoga.vi.691853

Research Article

Using Possibilities of New Lactic Acid Bacteria Isolates as Microbial Inoculant on Different Dry Matter Containing Alfalfa (Medicago sativa L.) Silage

Year 2020, Volume: 23 Issue: 5, 1331 - 1339, 31.10.2020

Mustafa Kızılsımsek , Kübra Keklik Tuğba Günaydın

https://doi.org/10.18016/ksutarimdoga.vi.691853

Cited By: 7

Abstract

In this study, inoculation effects of two lactic acid bacteria, L. bifermentans and L. brevis, isolated from flora of Turkey, on alfalfa silage containing 23.92% DM (unwilted), and 32.97% DM (wilted) were investigated. Result of the research indicated that wilting effected the fermentation profile significantly by decreasing the pH level and increasing dry matter preservation. Bacterial inoculation decreased the pH level, increased the production of lactic acid, whereas decreased the acetic acid, propionic acid and butyric acid in silage content. L. brevis strain as well as wilting can be used successfully in alfalfa silage production when crude protein and lactic acid production in silage were taken into consideration.

Keywords

Alfalfa , Inoculation , Wilting , Lactic acid bacteria

Project Number

117O854

References

Agarussi MCN, Pereira OG, da Silva VP, Leonardo ES, Ribeiro KG, Santos AA 2019. Fermentative Profile and Lactic Acid Bacterial Dynamics in Non-Wilted And Wilted Alfalfa Silage in Tropical Conditions. Molecular Biology Reports, (46): 451-460.
AOAC, 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists. Washington. DC.US.
Canbolat Ö, Akbay KC, Kamalak A 2019. Yem Bezelyesi Silajlarında Karbonhidrat Kaynağı Olarak Melas Kullanılma Olanakları. KSU Tar Doğa Derg 22(1) : 122-130.
Cavallarin L, Antoniazzi S, Borreani G, Tabacco E 2005. Effects of Wilting and Mechanical Conditioning on Proteolysis in Sainfoin (Onobrychis viciifolia Scop.) Wilted Herbage and Silage. J. Sci. Food Agric (85): 831- 838.
Dumlu Gül Z, Tan M, Fayetörbay Kaynar D, Kharazmi K 2015. Effects of Some Additives. Harvest stage and wilting on quality Characteristics of Alfalfa Silage. Atatürk Üniv J of the Agricultural Faculty 46 (2): 113-118.
Deriaz RE 1961. Routine Analysis of Carbohydrates and Lignin in Herbage. Journal of the Science of Food and Agriculture (12): 152-160.
Dordevic S, Mandic V, Stanojevic D 2016. The Effect Of Bacterial Inoculant on Chemical Composition And Fermentation of Alfalfa Silage. Biotechnology in Animal Husbandry 32(4): 431-423.
Ertekin İ, Kızılşimşek M 2020. Effects of Lactic Acid Bacteria Inoculation in Pre-Harvesting Period on Fermentation and Feed Quality of Alfalfa Silage. Asian-Australian Journal of Animal Science 33 (2): 245-253.
Jatkauskas J, Vrotniakiene V 2016. Using Special Inoculants Reduces Dry Matter Losses and Increases Fermentation Parameters of Lucerne Silage. Animal Husbandry, Scientific Articles (64): 3-11.
Khafipour E, Li S, Plaizier JC, Krause DO 2009. Rumen Microbiome Composition Determined Using Two Nutritional Models of Subacute Ruminal Acidosis. Applied and Environmental Microbiology (75): 7115-7124.
Kızılşimşek M, Schmidtt RJ, Kung L Jr 2007. Effects of A Mixture of Lactic Acid Bacteria Applied as a Freeze-Dried or Fresh Culture on the Fermentation of Alfalfa Silage. J Dairy Science 90(12): 5698-5705.
Koç F, Karapınar B, Okuyucu B, Korucu Erdem D 2020. Kefir İlavesinin Yonca Silajlarının Fermantasyon Özellikleri ve Aerobik Stabilitesi Üzerine Etkileri. KSU Tarım ve Doğa Derg 23(2): 535-542.
Liu C, Lai Y, Lu X, Guo P, Luo H 2016. Effects of Lactic Acid Bacteria Inoculants on Alfalfa (Medicago sativa L.) Silage Quality: Assessment of Degradation (in situ) and Gas Production (in vitro). Journal of Integrative Agriculture 15(12): 2834-2841.
Pitt RE, Muck RE, Leibensperger RY 1985. A Quantitative Model of The Ensilage Process in Lactate Silages. Grass Forage Science (40): 279-303
Quiros ARB, Yusty MAL, Hernandez JL 2009. HPLC Analysis of Organic Acids Using A Novel Stationary Phase. Talanta (78): 643-646.
Schmidt RJ, Hu W, Mills JA, Kung L Jr 2009. The Development of Lactic Acid Bacteria and Lactobacillus buchneri and Their Effects on The Fermentation of Alfalfa Silage. J Dairy Sci (92): 5005–5010.
Tao L, Zhou H, Zang N, Si B, Tu Y, Ma T, Diao Q 2017. Effects of Different Source Additives and Wilt Conditions on The pH Value, Aerobic Stability and Carbohydrate and Protein Fractions of Alfalfa Silage. Animal Science Journal (88): 99-106.
Tyrolova Y, Výborna A 2011. The Effects of Wilting and Biological and Chemical Additives on The Fermentation Process in Field Pea Silage. Czech J Anim Sci 56(10): 427–432.
TUIK 2017. Türkiye İstatistik Kurumu verileri.
Van Soest PJ, Robertson JD, Lewis BA 1991. Methods for Dietary Fibre, Neutral Detergent Fibre and Non-Starch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science (74): 3583–3597.
Virtanen AI, 1993. The A.I.V. Method of Preserving Fresh Fodder. Empire Journal of Exp Agric (1): 143-155.
Yang WZ, Beauchemin KA 2009. Increasing Physically Effective Fiber Content of Dairy Cow Diets Through Forage Proportion Versus Forage Chop Length: Chewing and Ruminal pH. Journal of Dairy Science (92): 1603-1615.
Zheng M, Niu D, Zuo S, Mao P, Meng L, Xu C 2017. The Effect of Cultivar, Wilting and Storage Period on Fermentation and the Clostridial Community of Alfalfa Silage. Italian Journal of Animal Science 17(2): 336-346.

Yeni Laktik Asit Bakteri İzolatlarının Farklı Kuru Madde İçeriğine Sahip Yonca (Medicago sativa L.) Silajında Mikrobiyel İnokulant Olarak Kullanılma Olanakları

Year 2020, Volume: 23 Issue: 5, 1331 - 1339, 31.10.2020

Mustafa Kızılsımsek , Kübra Keklik Tuğba Günaydın

https://doi.org/10.18016/ksutarimdoga.vi.691853

Cited By: 7

Abstract

Bu çalışmada, ülkemiz florasından izole edilen L. bifermentans ve L. brevis bakterilerinin, KM içeriği %23.92 olan ve soldurularak %32.97 seviyesine çıkarılan yonca materyallerine aşılanmasının silaj kalitesine olan etkileri incelenmiştir. Araştırma sonucunda, soldurmanın fermentasyon profili üzerine önemli etkilerinin olduğu, pH seviyesini düşürdüğü ve kuru madde kayıplarını azalttığı belirlenmiştir. Bakteri aşılamasının ise pH, asetik asit, propiyonik asit ve bütrik asit içeriğini düşürdüğü, laktik asit üretimini artırdığı belirlenmiştir. Araştırma sonucunda, ham protein içeriği ve laktik asit değerleri dikkate alındığında, yonca silajında soldurma ve L. brevis izolatı ile aşılamanın başarılı sonuçlar verdiği belirlenmiştir.

Keywords

Yonca , İnokulasyon , Soldurma , Laktik asit bakterisi

Supporting Institution

TUBİTAK 1002 projesi

Project Number

117O854

Thanks

Bu çalışma TUBİTAK 1002 projeleri kapsamında (117O854) desteklenmiştir.

References

Agarussi MCN, Pereira OG, da Silva VP, Leonardo ES, Ribeiro KG, Santos AA 2019. Fermentative Profile and Lactic Acid Bacterial Dynamics in Non-Wilted And Wilted Alfalfa Silage in Tropical Conditions. Molecular Biology Reports, (46): 451-460.
AOAC, 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists. Washington. DC.US.
Canbolat Ö, Akbay KC, Kamalak A 2019. Yem Bezelyesi Silajlarında Karbonhidrat Kaynağı Olarak Melas Kullanılma Olanakları. KSU Tar Doğa Derg 22(1) : 122-130.
Cavallarin L, Antoniazzi S, Borreani G, Tabacco E 2005. Effects of Wilting and Mechanical Conditioning on Proteolysis in Sainfoin (Onobrychis viciifolia Scop.) Wilted Herbage and Silage. J. Sci. Food Agric (85): 831- 838.
Dumlu Gül Z, Tan M, Fayetörbay Kaynar D, Kharazmi K 2015. Effects of Some Additives. Harvest stage and wilting on quality Characteristics of Alfalfa Silage. Atatürk Üniv J of the Agricultural Faculty 46 (2): 113-118.
Deriaz RE 1961. Routine Analysis of Carbohydrates and Lignin in Herbage. Journal of the Science of Food and Agriculture (12): 152-160.
Dordevic S, Mandic V, Stanojevic D 2016. The Effect Of Bacterial Inoculant on Chemical Composition And Fermentation of Alfalfa Silage. Biotechnology in Animal Husbandry 32(4): 431-423.
Ertekin İ, Kızılşimşek M 2020. Effects of Lactic Acid Bacteria Inoculation in Pre-Harvesting Period on Fermentation and Feed Quality of Alfalfa Silage. Asian-Australian Journal of Animal Science 33 (2): 245-253.
Jatkauskas J, Vrotniakiene V 2016. Using Special Inoculants Reduces Dry Matter Losses and Increases Fermentation Parameters of Lucerne Silage. Animal Husbandry, Scientific Articles (64): 3-11.
Khafipour E, Li S, Plaizier JC, Krause DO 2009. Rumen Microbiome Composition Determined Using Two Nutritional Models of Subacute Ruminal Acidosis. Applied and Environmental Microbiology (75): 7115-7124.
Kızılşimşek M, Schmidtt RJ, Kung L Jr 2007. Effects of A Mixture of Lactic Acid Bacteria Applied as a Freeze-Dried or Fresh Culture on the Fermentation of Alfalfa Silage. J Dairy Science 90(12): 5698-5705.
Koç F, Karapınar B, Okuyucu B, Korucu Erdem D 2020. Kefir İlavesinin Yonca Silajlarının Fermantasyon Özellikleri ve Aerobik Stabilitesi Üzerine Etkileri. KSU Tarım ve Doğa Derg 23(2): 535-542.
Liu C, Lai Y, Lu X, Guo P, Luo H 2016. Effects of Lactic Acid Bacteria Inoculants on Alfalfa (Medicago sativa L.) Silage Quality: Assessment of Degradation (in situ) and Gas Production (in vitro). Journal of Integrative Agriculture 15(12): 2834-2841.
Pitt RE, Muck RE, Leibensperger RY 1985. A Quantitative Model of The Ensilage Process in Lactate Silages. Grass Forage Science (40): 279-303
Quiros ARB, Yusty MAL, Hernandez JL 2009. HPLC Analysis of Organic Acids Using A Novel Stationary Phase. Talanta (78): 643-646.
Schmidt RJ, Hu W, Mills JA, Kung L Jr 2009. The Development of Lactic Acid Bacteria and Lactobacillus buchneri and Their Effects on The Fermentation of Alfalfa Silage. J Dairy Sci (92): 5005–5010.
Tao L, Zhou H, Zang N, Si B, Tu Y, Ma T, Diao Q 2017. Effects of Different Source Additives and Wilt Conditions on The pH Value, Aerobic Stability and Carbohydrate and Protein Fractions of Alfalfa Silage. Animal Science Journal (88): 99-106.
Tyrolova Y, Výborna A 2011. The Effects of Wilting and Biological and Chemical Additives on The Fermentation Process in Field Pea Silage. Czech J Anim Sci 56(10): 427–432.
TUIK 2017. Türkiye İstatistik Kurumu verileri.
Van Soest PJ, Robertson JD, Lewis BA 1991. Methods for Dietary Fibre, Neutral Detergent Fibre and Non-Starch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science (74): 3583–3597.
Virtanen AI, 1993. The A.I.V. Method of Preserving Fresh Fodder. Empire Journal of Exp Agric (1): 143-155.
Yang WZ, Beauchemin KA 2009. Increasing Physically Effective Fiber Content of Dairy Cow Diets Through Forage Proportion Versus Forage Chop Length: Chewing and Ruminal pH. Journal of Dairy Science (92): 1603-1615.
Zheng M, Niu D, Zuo S, Mao P, Meng L, Xu C 2017. The Effect of Cultivar, Wilting and Storage Period on Fermentation and the Clostridial Community of Alfalfa Silage. Italian Journal of Animal Science 17(2): 336-346.

There are 23 citations in total.

Details

Primary Language	Turkish
Subjects	Agricultural, Veterinary and Food Sciences
Journal Section	RESEARCH ARTICLE
Authors	Mustafa Kızılsımsek 0000-0002-0295-0603 Kübra Keklik 0000-0002-9527-288X Tuğba Günaydın 0000-0002-4458-1287
Project Number	117O854
Publication Date	October 31, 2020
Submission Date	February 20, 2020
Acceptance Date	May 1, 2020
Published in Issue	Year 2020 Volume: 23 Issue: 5

Cite

APA	Kızılsımsek, M., Keklik, K., & Günaydın, T. (2020). Yeni Laktik Asit Bakteri İzolatlarının Farklı Kuru Madde İçeriğine Sahip Yonca (Medicago sativa L.) Silajında Mikrobiyel İnokulant Olarak Kullanılma Olanakları. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(5), 1331-1339. https://doi.org/10.18016/ksutarimdoga.vi.691853