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Investigation of the Erythrocyte Fragility, Hematological and Antioxidant Effects of Oleander (Nerium oleander L.) Flower Ethanolic Lyophilized Extract in Diabetic Rats

Year 2020, Volume: 23 Issue: 6, 1495 - 1502, 31.12.2020

Abdulahad Doğan

https://doi.org/10.18016/ksutarimdoga.vi.712369
Cited By: 2

Abstract

The present study was focused on the erythrocyte fragility, hematological parameters, total antioxidant capacity (TAS), total oxidant capacity (TOS) and oxidative stress index (OSI) of flower ethanolic lyophilized extract obtained from N. oleander plant on Streptozotocin (50 mg kg-1)-induced Type 1 diabetes in rats. After toxicity test, 49 rats were divided into 7 groups. Control, Nerium oleander (NO) (50 mg kg-1), Diabetes mellitus (DM), DM+Gly-3 (3 mg kg-1), DM+NO-25 (25 mg kg-1), DM+NO-75 (75 mg kg-1) and DM+NO-225 (225 mg kg-1). Based on the results, after 21 days of application, erythrocyte hemolysis of DM group was increased (at 0.2-0.6% NaCI concentrations) compared to Control groups whereas treatment groups significantly decreased (P<0.05) erythrocyte hemolysis, especially in DM+NO-225 group. Although, hematological parameters showed some fluctuations in the groups, DM+Gly group showed significant increase (P<0.05) in erythrocyte (RBCs), hemoglobin (HGB), hematocrit (HCT) levels compared to all other groups. In addition, leukocyte (WBC), red cell distribution width (RDWs) and mean corpuscular volume (MCV) values of DM group increased with respect to the control groups whereas mean cell corpuscular hemoglobin concentration (MCHC) value significantly decreased (P<0.05). The decrease in plasma TAS level and increase in TOS and OSI values in the DM group were found significant compared to control and application groups. It was concluded that ethanolic lyophilized extract of N. oleander flowers prevented/decreased increased hemolysis in STZ-induced diabetic rats and also it could possess antioxidant properties.

Keywords

Nerium oleander Fragility Hematologic Antioxidant Diabetic Rat

Project Number

TAP-2018-7098

References

  • Abdou RH, Basha WA, Khalil WF 2019. Subacute Toxicity of Nerium oleander Ethanolic Extract in Mice. Toxicological research 35 (3): 233.
  • Al-Yahya MA, Al-Farhan AH, Adam SEI 2000. Preliminary toxicity study on the individual and combined effects of Citrullus colocynthis and Nerium oleander in rats. Fitoterapia 71(4): 385-391.
  • American Diabetes Association. 2018. 2. Classification and diagnosis of diabetes: standards of medical care in diabetes-2018. Diabetes care 41 (Supplement 1): S13-S27.
  • Bandara V, Weinstein SA, White J, Eddleston M 2010. A review of the natural history, toxinology, diagnosis and clinical management of Nerium oleander (common oleander) and Thevetia peruviana (yellow oleander) poisoning. Toxicon 56: 273–281.
  • Bavunoğlu I, Balta M, Türkmen Z 2016. Oleander poisoning as an example of self-medication attempt. Balkan medical journal 33 (5): 559.
  • Baytop T, Baytop A, Mat A, Sun S 1989. Türkiyede zehirli bitkiler, bitki zehirlenmeleri ve tedavi yöntemleri. İstanbul Üniversitesi Yayınları, 3560, 975- 404-111-3.
  • Boswell BR, Dorweiler MA, Erbs NC, Chm B, Caplan JP 2013. A case of Nerium oleander toxicity: a thorny predicament. Psychosomatics (Washington, DC) 54 (4): 379-381.
  • Botelho AFM, Santos-Miranda A, Joca HC, Mattoso CRS, de Oliveira MS, Pierezan F, Cruz JS, Soto-Blanco B, Melo MM 2017. Hydroalcoholic extract from Nerium oleander L. (Apocynaceae) elicits arrhythmogenic activity. Journal of ethnopharmacology 206: 170-177.
  • Dalar A, Dogan A, Bengu AS, Mukemre M, Celik I 2018. Screening in vivo antioxidant and haematological properties of sumac and acorn bioactive rich extracts. Industrial Crops and Products 124: 20-27.
  • Dey P, Saha MR, Chowdhuri SR, Sen A, Sarkar MP, Haldar B, Chaudhuri TK 2015. Assessment of anti-diabetic activity of an ethnopharmacological plant Nerium oleander through alloxan induced diabetes in mice. Journal of ethnopharmacology 161: 128-137.
  • Dogan A, Celik I, Kaya MS 2015. Antidiabetic properties of lyophilized extract of acorn (Quercus brantii Lindl.) on experimentally STZ-induced diabetic rats. Journal of ethnopharmacology 176: 243-251.
  • Dogan A, Dalar A, Sadullahoglu C, Battal A, Uzun Y, Celik I, Demirel K 2018. Investigation of the protective effects of horse mushroom (Agaricus arvensis Schaeff.) against carbon tetrachloride-induced oxidative stress in rats. Molecular biology reports 45(5): 787-797.
  • Doğan A 2018. CCI4 İle oksidatif stres oluşturulan sıçanlarda Pholiota aurivella liyofilize ekstrenin bazı biyokimyasal ve hematolojik parametrelere etkisi. Mantar Dergisi 9 (1): 58-66.
  • Erel O 2004. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry 37(4): 277–285.
  • Erel O 2005. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry 38(12): 1103–1111.
  • Ergun B 1992. Nerium oleander’in bazı suda çözünen bileşiklerinin in vitro biyolojik etkileri. Anadolu Üniversitesi Sağlık Bilimleri Enstitüsü Farmakoloji Anabilim Dalı, Doktora Tezi, 56 sy.
  • Farkhondeh T, Kianmehr M, Kazemi T, Samarghandian S, Khazdair MR 2020. Toxicity effects of Nerium oleander, basic and clinical evidence: A comprehensive review. Human & Experimental Toxicology 0960327120901571.
  • Gürgen A, Sevindik M, Yıldız S, Akgül H 2020. Determination of Antioxidant and Oxidant Potentials of Pleurotus citrinopileatus Mushroom Cultivated on Various Substrates. KSU J. Agric Nat 23(3), 586-591.
  • Hase GJ, Deshmukh KK, Murade VD, Pokharkar RD, Phatanagre ND, Hase DP, Dichayal S, Gosavi AB 2016. Phytopharmacology of Nerium oleander L. A review. International Journal of Phytopharmacology 7 (2): 0975-9328.
  • Inci A, Olmaz R, Sarı F, Coban M, Ellidag HY, Sarıkaya M 2016. Increased oxidative stress in diabetic nephropathy and its relationship with soluble Klotho levels. Hippokratia 20 (3): 198.
  • Kgosana KG 2019. The effects of extraction techniques and quantitative determination of oxalates in Nerium oleander and feeds. Onderstepoort Journal of Veterinary Research 86 (1): 1-9.
  • Kumar A, De T, Mishra A, Mishra AK 2013. Oleandrin: A cardiac glycosides with potent cytotoxicity. Pharmacognosy reviews 7 (14): 131.
  • Kumar D, Al Hassan M, Naranjo MA, Agrawal V, Boscaiu M, Vicente O 2017. Effects of salinity and drought on growth, ionic relations, compatible solutes and activation of antioxidant systems in oleander (Nerium oleander L.). Plos one 12 (9).
  • Kung CM, Tseng ZL, Wang HL 2009. Erythrocyte fragility increases with level of glycosylated hemoglobin in type 2 diabetic patients. Clinical hemorheology and microcirculation 43 (4): 345-351.
  • Miikue-Yobe TFB 2015. Effect of aqueous leaf extract of Heinsia crinata on haematological and some biochemical indices of toxicity in streptozotocin induced diabetic rats. International Journal for Innovative Research in Science & Technology 2: 116-126.
  • Pradeep SR, Srinivasan K 2018. Haemato-protective influence of dietary fenugreek (Trigonella foenum-graecum L.) seeds is potentiated by onion (Allium cepa L.) in streptozotocin-induced diabetic rats. Biomedicine & Pharmacotherapy 98: 372-381.
  • Sureka C, Ramesh T, Begum VH 2015. Attenuation of erythrocyte membrane oxidative stress by Sesbania grandiflora in streptozotocin-induced diabetic rats. Biochemistry and Cell Biology 93 (4): 385-395.
  • Thomas MC, MacIsaac RJ, Tsalamandris C, Power D, Jerums G 2003. Unrecognized anemia in patients with diabetes: a cross-sectional survey. Diabetes care 26 (4): 1164-1169.

Diyabetik Ratlarda Zakkum (Nerium oleander L.) Çiçeği Etanolik Liyofilize Ekstresinin Eritrosit Frajilite, Hematolojik ve Antioksidan Etkilerinin Araştırılması

Year 2020, Volume: 23 Issue: 6, 1495 - 1502, 31.12.2020

Abdulahad Doğan

https://doi.org/10.18016/ksutarimdoga.vi.712369
Cited By: 2

Abstract

Bu çalışma streptozotosin (50 mg kg-1) ile Tip 1 diyabet oluşturulan ratlarda zakkum (N. oleander) çiçeğinden elde edilen etanolik liyofilize ekstresinin eritrosit frajilite, hematolojik parametreler, total antioksidan kapasite (TAS), total oksidan kapasite (TOS) ve oksidatif stress indeksi (OSI) üzerine olan etkilerine odaklandı. Toksisite testi sonrası, 49 rat 7 gruba ayrıldı. Kontrol, Nerium oleander (NO) (50 mg kg-1), Diabetes mellitus (DM), DM+Gly-3 (3 mg kg-1), DM+NO-25 (25 mg kg-1), DM+NO-75 (75 mg kg-1) ve DM+NO-225 (225 mg kg-1). 21 günlük uygulamadan sonra elde edilen bulgulara göre, DM grubunda eritrosit hemolizi (%0.2-0.6 NaCI konsantrasyonlarda) kontrol gruplarına göre önemli artış gösterirken (P<0.05); özellikle DM+NO-225 grubunda eritrosit hemolizi önemli oranda azaldı (P<0.05). Hematolojik parametreler, bazı gruplarda dalgalanmalar göstermesine rağmen, DM+Gly grubunda eritrosit (RBC), hemoglobin (HGB) ve hematokrit (HCT) düzeyleri diğer gruplara göre önemli artış gösterdi (P<0.05). Ayrıca, DM grubu lökosit (WBC), eritrosit dağılım genişliği (RDW) ve ortalama eritrosit hacmi (MCV) değerleri kontrol gruplarına göre önemli artış gösterirken, eritrosit ortalama hemoglobin konsantrasyonu (MCHC) ise önemli oranda azaldı (P<0.05). DM grubunda plazma TAS seviyesindeki azalma, ile TOS ve OSI değerindeki artış kontrol ve uygulama gruplarına göre önemli bulundu. N. oleander çiçek liyofilize ekstresinin STZ ile diyabet oluşturulan ratlarda diyabetin sebep olduğu artan hemolizi önleyici/azaltıcı etkilere ve antioksidan özelliklere sahip olabileceği sonucuna varılmıştır. 

Keywords

Nerium oleander Frajilite Hematolojik Antioksidan Diyabetik Rat

Supporting Institution

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Project Number

TAP-2018-7098

Thanks

Bu araştırma Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı tarafından TAP-2018-7098 numaralı proje olarak desteklenmiştir. Ayrıca bitkiyi teşhis eden sayın Doç. Dr. Süleyman Mesut PINAR hocamıza teşekkürü bir borç bilirim.

References

  • Abdou RH, Basha WA, Khalil WF 2019. Subacute Toxicity of Nerium oleander Ethanolic Extract in Mice. Toxicological research 35 (3): 233.
  • Al-Yahya MA, Al-Farhan AH, Adam SEI 2000. Preliminary toxicity study on the individual and combined effects of Citrullus colocynthis and Nerium oleander in rats. Fitoterapia 71(4): 385-391.
  • American Diabetes Association. 2018. 2. Classification and diagnosis of diabetes: standards of medical care in diabetes-2018. Diabetes care 41 (Supplement 1): S13-S27.
  • Bandara V, Weinstein SA, White J, Eddleston M 2010. A review of the natural history, toxinology, diagnosis and clinical management of Nerium oleander (common oleander) and Thevetia peruviana (yellow oleander) poisoning. Toxicon 56: 273–281.
  • Bavunoğlu I, Balta M, Türkmen Z 2016. Oleander poisoning as an example of self-medication attempt. Balkan medical journal 33 (5): 559.
  • Baytop T, Baytop A, Mat A, Sun S 1989. Türkiyede zehirli bitkiler, bitki zehirlenmeleri ve tedavi yöntemleri. İstanbul Üniversitesi Yayınları, 3560, 975- 404-111-3.
  • Boswell BR, Dorweiler MA, Erbs NC, Chm B, Caplan JP 2013. A case of Nerium oleander toxicity: a thorny predicament. Psychosomatics (Washington, DC) 54 (4): 379-381.
  • Botelho AFM, Santos-Miranda A, Joca HC, Mattoso CRS, de Oliveira MS, Pierezan F, Cruz JS, Soto-Blanco B, Melo MM 2017. Hydroalcoholic extract from Nerium oleander L. (Apocynaceae) elicits arrhythmogenic activity. Journal of ethnopharmacology 206: 170-177.
  • Dalar A, Dogan A, Bengu AS, Mukemre M, Celik I 2018. Screening in vivo antioxidant and haematological properties of sumac and acorn bioactive rich extracts. Industrial Crops and Products 124: 20-27.
  • Dey P, Saha MR, Chowdhuri SR, Sen A, Sarkar MP, Haldar B, Chaudhuri TK 2015. Assessment of anti-diabetic activity of an ethnopharmacological plant Nerium oleander through alloxan induced diabetes in mice. Journal of ethnopharmacology 161: 128-137.
  • Dogan A, Celik I, Kaya MS 2015. Antidiabetic properties of lyophilized extract of acorn (Quercus brantii Lindl.) on experimentally STZ-induced diabetic rats. Journal of ethnopharmacology 176: 243-251.
  • Dogan A, Dalar A, Sadullahoglu C, Battal A, Uzun Y, Celik I, Demirel K 2018. Investigation of the protective effects of horse mushroom (Agaricus arvensis Schaeff.) against carbon tetrachloride-induced oxidative stress in rats. Molecular biology reports 45(5): 787-797.
  • Doğan A 2018. CCI4 İle oksidatif stres oluşturulan sıçanlarda Pholiota aurivella liyofilize ekstrenin bazı biyokimyasal ve hematolojik parametrelere etkisi. Mantar Dergisi 9 (1): 58-66.
  • Erel O 2004. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry 37(4): 277–285.
  • Erel O 2005. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry 38(12): 1103–1111.
  • Ergun B 1992. Nerium oleander’in bazı suda çözünen bileşiklerinin in vitro biyolojik etkileri. Anadolu Üniversitesi Sağlık Bilimleri Enstitüsü Farmakoloji Anabilim Dalı, Doktora Tezi, 56 sy.
  • Farkhondeh T, Kianmehr M, Kazemi T, Samarghandian S, Khazdair MR 2020. Toxicity effects of Nerium oleander, basic and clinical evidence: A comprehensive review. Human & Experimental Toxicology 0960327120901571.
  • Gürgen A, Sevindik M, Yıldız S, Akgül H 2020. Determination of Antioxidant and Oxidant Potentials of Pleurotus citrinopileatus Mushroom Cultivated on Various Substrates. KSU J. Agric Nat 23(3), 586-591.
  • Hase GJ, Deshmukh KK, Murade VD, Pokharkar RD, Phatanagre ND, Hase DP, Dichayal S, Gosavi AB 2016. Phytopharmacology of Nerium oleander L. A review. International Journal of Phytopharmacology 7 (2): 0975-9328.
  • Inci A, Olmaz R, Sarı F, Coban M, Ellidag HY, Sarıkaya M 2016. Increased oxidative stress in diabetic nephropathy and its relationship with soluble Klotho levels. Hippokratia 20 (3): 198.
  • Kgosana KG 2019. The effects of extraction techniques and quantitative determination of oxalates in Nerium oleander and feeds. Onderstepoort Journal of Veterinary Research 86 (1): 1-9.
  • Kumar A, De T, Mishra A, Mishra AK 2013. Oleandrin: A cardiac glycosides with potent cytotoxicity. Pharmacognosy reviews 7 (14): 131.
  • Kumar D, Al Hassan M, Naranjo MA, Agrawal V, Boscaiu M, Vicente O 2017. Effects of salinity and drought on growth, ionic relations, compatible solutes and activation of antioxidant systems in oleander (Nerium oleander L.). Plos one 12 (9).
  • Kung CM, Tseng ZL, Wang HL 2009. Erythrocyte fragility increases with level of glycosylated hemoglobin in type 2 diabetic patients. Clinical hemorheology and microcirculation 43 (4): 345-351.
  • Miikue-Yobe TFB 2015. Effect of aqueous leaf extract of Heinsia crinata on haematological and some biochemical indices of toxicity in streptozotocin induced diabetic rats. International Journal for Innovative Research in Science & Technology 2: 116-126.
  • Pradeep SR, Srinivasan K 2018. Haemato-protective influence of dietary fenugreek (Trigonella foenum-graecum L.) seeds is potentiated by onion (Allium cepa L.) in streptozotocin-induced diabetic rats. Biomedicine & Pharmacotherapy 98: 372-381.
  • Sureka C, Ramesh T, Begum VH 2015. Attenuation of erythrocyte membrane oxidative stress by Sesbania grandiflora in streptozotocin-induced diabetic rats. Biochemistry and Cell Biology 93 (4): 385-395.
  • Thomas MC, MacIsaac RJ, Tsalamandris C, Power D, Jerums G 2003. Unrecognized anemia in patients with diabetes: a cross-sectional survey. Diabetes care 26 (4): 1164-1169.
There are 28 citations in total.

Details

Primary Language Turkish
Subjects Structural Biology
Journal Section RESEARCH ARTICLE
Authors

Abdulahad Doğan 0000-0002-5438-8560

Project Number TAP-2018-7098
Publication Date December 31, 2020
Submission Date March 31, 2020
Acceptance Date May 14, 2020
Published in Issue Year 2020 Volume: 23 Issue: 6

Cite

APA Doğan, A. (2020). Diyabetik Ratlarda Zakkum (Nerium oleander L.) Çiçeği Etanolik Liyofilize Ekstresinin Eritrosit Frajilite, Hematolojik ve Antioksidan Etkilerinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(6), 1495-1502. https://doi.org/10.18016/ksutarimdoga.vi.712369

Cited By

Achillea arabica Kotschy. Liyolifilize Etanolik Ekstresinin Streptozotosin ile Diyabet Oluşturulan Ratların Adacık β Hücreleri Üzerindeki Koruyucu Etkileri
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
Hanife HANALP
https://doi.org/10.18016/ksutarimdoga.vi.811253
Kangal Çoban Köpeklerinde Yaş ve Cinsiyetin Bazı Hematolojik Parametreler ve Eritrosit Ozmotik Kırılganlık Üzerine Etkisi
Atatürk Üniversitesi Veteriner Bilimleri Dergisi
https://doi.org/10.17094/ataunivbd.1051477
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