Evaluation of The Effects of Pomegranate Juice on Hepato-Nephrotoxicity in Male Rats Exposed to Aluminum

Harun Çiftçi; Çiğdem Er Çalışkan; Fatih Kar; Ahmet Özkaya; Kenan Güçlü

doi:10.18016/ksutarimdoga.vi.896611

Research Article

Alüminyuma Maruz Kalmış Erkek Sıçanlarda Nar Suyunun Hepato-Nefrotoksisite Üzerine Etkilerinin Değerlendirilmesi.

Year 2022, Volume: 25 Issue: 2, 215 - 222, 30.04.2022

Harun Çiftçi , Çiğdem Er Çalışkan , Fatih Kar , Ahmet Özkaya , Kenan Güçlü

https://doi.org/10.18016/ksutarimdoga.vi.896611

Cited By: 4

Abstract

Alüminyum, günümüz aşı çalışmalarında stabilizatör ve efektör olarak kullanılmaktadır. Alüminyum tüm hayatımızda olmasına rağmen kimyasal özellikler açısından uygun olmayan özelliklere sahiptir. Narın önemli biyolojik ve tıbbi özellikleri vardır. Nar suyunun AlCl3 kaynaklı hepato-nefrotoksisiteye olan etkilerini analiz etmek için sıçan serumundaki elektrolit seviyeleri, böbrek ve karaciğer fonksiyon testi ve lipid profili gibi biyokimyasal parametreleri amaçlanmıştır. Yirmi sekiz wistar albino sıçan; kontrol (Grup I), nar suyu (4 ml/kg) (Grup II), AlCl3 (8,3 mg/kg) (Grup III) ve nar suyu + AlCl3 (Grup IV) şeklinde dört gruba ayrılmıştır. Alüminyum toksisitesi ve nar suyunun koruyucu etkisi elektrolit düzeyleri açısından Mg ve Cl hariç gruplar arasında istatistiksel olarak farklı bulunmamıştır (p <0.05). AlCl3, serum AST, ALT, BUN ve CREA düzeylerini önemli ölçüde artırdığı ve ALB ve TP'yi düşürdüğü (p <0.05) görülmüştür. AlCl3 ile birlikte veya öncesinde nar suyu uygulaması, karaciğer ve böbrek fonksiyonunun serum biyobelirteçlerini neredeyse normale yakın seviyelere getirdiği görülmüştür. Lipid metabolizmasında sadece LDL ve CHOL düzeylerinde istatistiksel olarak anlamlı bir fark görülmüştür (p <0.05). Nar suyu, elektrolit seviyeleri, karaciğer ve böbrek fonksiyon testi ve lipid profili açısından alüminyum toksisitesini azalttığı sonucuna varılmıştır.

Keywords

Alüminyum , Nar , Aşı , Böbrek-karaciğer toksisitesi , Elektrolit

References

Abd Elmonem HA 2014. Assessment the effect of pomegranate molasses against diazinon toxicity in male rats. IOSR Journal of Environmental Science, Toxicology and Food Technology 8(2): 135-141.
Aboulgasem GJ, Azab AE 2015. The potential protective effects of pomegranate juice against (S)-(-)-1-methyl-2-(3-pyridyl) pyrrolidine (+)-bitartrate salt induced serum biochemical changes in rabbits. International Journal of Science and Research 4(11): 360-371.
Al Dera HS 2016. Protective effect of resveratrol against aluminum chloride induced nephrotoxicity in rats. Saudi Medical Journal 37(4): 369–378
Al-Hashem F 2009. Camel’s milk protects against aluminum chloride-induced toxicity in the liver and kidney ofwhite albino rats. American Journal of Biochemistry and Biotechnology 5(3): 98–109.
Azza AA, Toqa ME, Abeer IAEF, Mona MK, Karema AE 2017. Comparative Study On The Influence Of Different Drugs And Nutrients Against Aluminium- Induced Nephrotoxicity And Hepatotoxicity In Rats. International Journal of Current Research 9(10): 58684-700
Balgoon MJ 2019. Assessment of the protective effect of Lepidium sativum against aluminum-induced liver and kidney effects in albino rat. BioMed Research International 4516730.
Celik H, Celik N, Kocyigit A, Dikilitas M 2012. The relationship between plasma aluminum content, lymphocyte DNA damage, and oxidative status in persons using aluminium containers and utensils daily. Clinical Biochemistry 45(18): 1629–1633.
El Kar C, Ferchichi A, Attia F, Bouajila J 2011. Pomegranate (Punica granatum) Juices: Chemical composition, micronutrient cations, and antioxidant capacity. J Food Sci 76(6): C795-800.
Esmaillzadeh A, Tahbaz F, Gaieni I, Alavi-Majd H, Azadbakht L 2006. Cholesterol‑lowering effect of concentrated pomegranate juice consumption in type II diabetic patients with hyperlipidemia. International Journal for Vitamin and Nutrition Research, 76: 147‑151.
Exley C 2013. Human exposure to aluminium. Environmental Science: Processes & Impacts 15: 1807–1816.
Ghorbel I, Elwej A, Chaabane M, Jamoussi, K, Zeghal N 2017. Protective effect of selenium against aluminium chloride induced cardiotoxicity in rats. Pharmaceutical and Biomedical Research 3(2): 19–25.
Hadipour JM, Mozaffari KR 2010. Chondroprotective effects of pomegranate juice on monoiodoacetate‐induced osteoarthritis of the knee joint of mice. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 24(2): 182-185.
Hassanen EI, Tohamy AF, Issa MY, Ibrahim MA, Farroh KY, Hassan AM 2019. Pomegranate Juice Diminishes The Mitochondria-Dependent Cell Death And NF-kB Signaling Pathway Induced By Copper Oxide Nanoparticles On Liver And Kidneys of Rats. International Journal of Nanomedicine 14: 8905.
Hotez PJ, Corry DB, Strych U, Bottazzi ME 2020. COVID-19 vaccines: neutralizing antibodies and the alum advantage. Nature Reviews Immunology 20: 399-400.
Hozayen WG, Soliman HAE, Desouky EM 2014. Potential protective effects of rosemary extract, against aspartame toxicity in male rats. Journal of International Academic Research for Multidisciplinary. 2(6): 111-125.
Ilbey YO, Ozbek E, Simsek A, Cekmen M, Somay A, Tasci AI 2009. Effects of pomegranate juice on hyperoxaluria-induced oxidative stress in the rat kidneys. Renal failure 31(6): 522-531.
Imam TS, Khalifa HA, Hussein MM, Ali HA 2016. Aluminum–induced oxidative stress and hepato-renal impairment in male albino rats: possible protective trial with naringenin, Life Science Journal 13(1): 93-104.
Kar F, Hacioglu C, Uslu S, Kanbak G 2019. Curcumin Acts as Post-protective Effects on Rat Hippocampal Synaptosomes in a Neuronal Model of Aluminum-Induced Toxicity. Neurochemical research 44(8): 2020-2029.
Manthou E, Georgakouli K, Deli CK, Sotiropoulos A, Fatouros IG, Kouretas D, Jamurtas AZ 2017. Effect of pomegranate juice consumption on biochemical parameters and complete blood count. Experimental and therapeutic medicine 14(2): 1756-1762.
Matthaiou CM, Goutzourelas N, Stagos D, Sarafoglou E, Jamurtas A, Koulocheri SD, Kouretas, D 2014. Pomegranate juice consumption increases GSH levels and reduces lipid and protein oxidation in human blood. Food and Chemical Toxicology 73: 1-6.
Moneim AEA, Dkhil MA, Al-Quraishy S 2011. Studies on the effect of pomegranate (Punica granatum) juice and peel on liver and kidney in adult male rats. Journal of Medicinal Plants Research, 5(20): 5083-5088.
Moneim AEA, El-Khadragy MF 2013. The potential effects of pomegranate (Punica granatum) juice on carbon tetrachloride-induced nephrotoxicity in rats. Journal of physiology and biochemistry, 69(3): 359-370.
Nayak P 2002. Aluminum: impacts & diseases. Environmental Research 89: 101–115.
Niedworok J, Fijałkowski P 2004. Effect of Long-Term Aluminium Chloride Intoxication on Selected Biochemical Parameters and Oxidative--Antioxidative Balance in Experimental Animals. Polish Journal of Environmental Studies 13(1): 41-3.
Ozkaya A, Celik S, Yuce A, Şahin Z, Yilmaz Ö 2010. The effects of ellagic acid on some biochemical parameters in the liver of rats against oxidative stress induced by aluminum. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16 (2): 263-268.
Parasuraman S, Qin BNY, Hui LC, Benk JYK 2020. Effect of epigallocatechin gallate on aluminum chloride-induced changes in behavior, biochemical parameters, and spermatogenesis of Sprague-Dawley rats. Beni-Suef University Journal of Basic and Applied Sciences 9(1): 1-10.
Parvin F, Monsefi M, Talaei KT 2014. Effect of Pomegranate Juice on Bone Calcium Content and Body Weight of Adult Mice. Zahedan Journal of Research in Medical Sciences 16(3): 64-66.
Pirinççioğlu M, Kızıl G, Kızıl M, Kanay Z, Ketani A 2014. The protective role of pomegranate juice against carbon tetrachloride–induced oxidative stress in rats. Toxicology and industrial health 30(10): 910-918.
Priest ND, Talbot RJ, Newton D, Day JP, King SJ, Fifield LK 1998. Uptake by man of aluminium in a public water supply. Hum Exp Toxicol 17: 296–301.
Samir AM, Rashed LA 2018. Effects of occupational exposure to aluminium on some oxidative stress and DNA damage parameters. Human & experimental toxicology 37(9): 901-908.
Stowe CB 2011. The effects of pomegranate juice consumption on blood pressure and cardiovascular health. Complementary Therapies in Clinical Practice 17(2): 113-5.
Sumathi T, Shobana C, Kumari BR, Nandhini, DN 2011. Protective role of Cynodon dactylon in ameliorating the aluminium-induced neurotoxicity in rat brain regions. Biological trace element research 144(1-3): 843-853.
Sumner MD, Elliott‑Eller M, Weidner G, Daubenmier JJ, Chew MH, Marlin R, Ornish D 2005. Effects of pomegranate juice consumption on myocardial perfusion in patients with coronary heart disease. American Journal of Cardiology, 96: 810‑814.
Vidal A, Fallarero A, Peña BR, Medina ME, Gra B, Rivera F, Vuorela PM 2003. Studies on the toxicity of Punica granatum L. (Punicaceae) whole fruit extracts. Journal of Ethnopharmacology 89: 295 300.

Evaluation of The Effects of Pomegranate Juice on Hepato-Nephrotoxicity in Male Rats Exposed to Aluminum

Year 2022, Volume: 25 Issue: 2, 215 - 222, 30.04.2022

Harun Çiftçi , Çiğdem Er Çalışkan , Fatih Kar , Ahmet Özkaya , Kenan Güçlü

https://doi.org/10.18016/ksutarimdoga.vi.896611

Cited By: 4

Abstract

Aluminum is used as a stabilizer and effector in today's vaccine studies. Although aluminum is in all our lives, it has unsuitable properties in terms of chemical properties. Pomegranate has notable biological and medicinal properties. We aimed to measure biochemical parameters in rat serum such as electrolyte levels, kidney and liver function test, and lipid profile to analyze the effects of pomegranate juice towards AlCl3-induced hepato-nephrotoxicity. The twenty-eight wistar albino rats divided into four groups; control (Group I), pomegranate juice (4 ml/kg) (Group II), AlCl3 (8.3 mg/kg) (Group III) and pomegranate juice + AlCl3 (Group IV). Aluminum toxicity and the protective effect of pomegranate juice did not statistically different among groups in electrolyte levels, except for Mg and Cl (p<0.05). AlCL3 considerably increased serum AST, ALT, BUN and CREA levels and decreased ALB and TP (p<0.05). Pomegranate juice administration with or before AlCl3 notably restored serum biomarkers of liver and kidney function to near-normal levels. There was only a statistically significant difference in LDL and CHOL levels in lipid metabolism (p<0.05). The pomegranate juice reduced aluminum toxicity in terms of electrolyte levels, liver and renal function test, and lipid profile.

Keywords

Aluminum , Pomegranate , Vaccine , Renal-liver toxicity , Electrolyte

References

Abd Elmonem HA 2014. Assessment the effect of pomegranate molasses against diazinon toxicity in male rats. IOSR Journal of Environmental Science, Toxicology and Food Technology 8(2): 135-141.
Aboulgasem GJ, Azab AE 2015. The potential protective effects of pomegranate juice against (S)-(-)-1-methyl-2-(3-pyridyl) pyrrolidine (+)-bitartrate salt induced serum biochemical changes in rabbits. International Journal of Science and Research 4(11): 360-371.
Al Dera HS 2016. Protective effect of resveratrol against aluminum chloride induced nephrotoxicity in rats. Saudi Medical Journal 37(4): 369–378
Al-Hashem F 2009. Camel’s milk protects against aluminum chloride-induced toxicity in the liver and kidney ofwhite albino rats. American Journal of Biochemistry and Biotechnology 5(3): 98–109.
Azza AA, Toqa ME, Abeer IAEF, Mona MK, Karema AE 2017. Comparative Study On The Influence Of Different Drugs And Nutrients Against Aluminium- Induced Nephrotoxicity And Hepatotoxicity In Rats. International Journal of Current Research 9(10): 58684-700
Balgoon MJ 2019. Assessment of the protective effect of Lepidium sativum against aluminum-induced liver and kidney effects in albino rat. BioMed Research International 4516730.
Celik H, Celik N, Kocyigit A, Dikilitas M 2012. The relationship between plasma aluminum content, lymphocyte DNA damage, and oxidative status in persons using aluminium containers and utensils daily. Clinical Biochemistry 45(18): 1629–1633.
El Kar C, Ferchichi A, Attia F, Bouajila J 2011. Pomegranate (Punica granatum) Juices: Chemical composition, micronutrient cations, and antioxidant capacity. J Food Sci 76(6): C795-800.
Esmaillzadeh A, Tahbaz F, Gaieni I, Alavi-Majd H, Azadbakht L 2006. Cholesterol‑lowering effect of concentrated pomegranate juice consumption in type II diabetic patients with hyperlipidemia. International Journal for Vitamin and Nutrition Research, 76: 147‑151.
Exley C 2013. Human exposure to aluminium. Environmental Science: Processes & Impacts 15: 1807–1816.
Ghorbel I, Elwej A, Chaabane M, Jamoussi, K, Zeghal N 2017. Protective effect of selenium against aluminium chloride induced cardiotoxicity in rats. Pharmaceutical and Biomedical Research 3(2): 19–25.
Hadipour JM, Mozaffari KR 2010. Chondroprotective effects of pomegranate juice on monoiodoacetate‐induced osteoarthritis of the knee joint of mice. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 24(2): 182-185.
Hassanen EI, Tohamy AF, Issa MY, Ibrahim MA, Farroh KY, Hassan AM 2019. Pomegranate Juice Diminishes The Mitochondria-Dependent Cell Death And NF-kB Signaling Pathway Induced By Copper Oxide Nanoparticles On Liver And Kidneys of Rats. International Journal of Nanomedicine 14: 8905.
Hotez PJ, Corry DB, Strych U, Bottazzi ME 2020. COVID-19 vaccines: neutralizing antibodies and the alum advantage. Nature Reviews Immunology 20: 399-400.
Hozayen WG, Soliman HAE, Desouky EM 2014. Potential protective effects of rosemary extract, against aspartame toxicity in male rats. Journal of International Academic Research for Multidisciplinary. 2(6): 111-125.
Ilbey YO, Ozbek E, Simsek A, Cekmen M, Somay A, Tasci AI 2009. Effects of pomegranate juice on hyperoxaluria-induced oxidative stress in the rat kidneys. Renal failure 31(6): 522-531.
Imam TS, Khalifa HA, Hussein MM, Ali HA 2016. Aluminum–induced oxidative stress and hepato-renal impairment in male albino rats: possible protective trial with naringenin, Life Science Journal 13(1): 93-104.
Kar F, Hacioglu C, Uslu S, Kanbak G 2019. Curcumin Acts as Post-protective Effects on Rat Hippocampal Synaptosomes in a Neuronal Model of Aluminum-Induced Toxicity. Neurochemical research 44(8): 2020-2029.
Manthou E, Georgakouli K, Deli CK, Sotiropoulos A, Fatouros IG, Kouretas D, Jamurtas AZ 2017. Effect of pomegranate juice consumption on biochemical parameters and complete blood count. Experimental and therapeutic medicine 14(2): 1756-1762.
Matthaiou CM, Goutzourelas N, Stagos D, Sarafoglou E, Jamurtas A, Koulocheri SD, Kouretas, D 2014. Pomegranate juice consumption increases GSH levels and reduces lipid and protein oxidation in human blood. Food and Chemical Toxicology 73: 1-6.
Moneim AEA, Dkhil MA, Al-Quraishy S 2011. Studies on the effect of pomegranate (Punica granatum) juice and peel on liver and kidney in adult male rats. Journal of Medicinal Plants Research, 5(20): 5083-5088.
Moneim AEA, El-Khadragy MF 2013. The potential effects of pomegranate (Punica granatum) juice on carbon tetrachloride-induced nephrotoxicity in rats. Journal of physiology and biochemistry, 69(3): 359-370.
Nayak P 2002. Aluminum: impacts & diseases. Environmental Research 89: 101–115.
Niedworok J, Fijałkowski P 2004. Effect of Long-Term Aluminium Chloride Intoxication on Selected Biochemical Parameters and Oxidative--Antioxidative Balance in Experimental Animals. Polish Journal of Environmental Studies 13(1): 41-3.
Ozkaya A, Celik S, Yuce A, Şahin Z, Yilmaz Ö 2010. The effects of ellagic acid on some biochemical parameters in the liver of rats against oxidative stress induced by aluminum. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16 (2): 263-268.
Parasuraman S, Qin BNY, Hui LC, Benk JYK 2020. Effect of epigallocatechin gallate on aluminum chloride-induced changes in behavior, biochemical parameters, and spermatogenesis of Sprague-Dawley rats. Beni-Suef University Journal of Basic and Applied Sciences 9(1): 1-10.
Parvin F, Monsefi M, Talaei KT 2014. Effect of Pomegranate Juice on Bone Calcium Content and Body Weight of Adult Mice. Zahedan Journal of Research in Medical Sciences 16(3): 64-66.
Pirinççioğlu M, Kızıl G, Kızıl M, Kanay Z, Ketani A 2014. The protective role of pomegranate juice against carbon tetrachloride–induced oxidative stress in rats. Toxicology and industrial health 30(10): 910-918.
Priest ND, Talbot RJ, Newton D, Day JP, King SJ, Fifield LK 1998. Uptake by man of aluminium in a public water supply. Hum Exp Toxicol 17: 296–301.
Samir AM, Rashed LA 2018. Effects of occupational exposure to aluminium on some oxidative stress and DNA damage parameters. Human & experimental toxicology 37(9): 901-908.
Stowe CB 2011. The effects of pomegranate juice consumption on blood pressure and cardiovascular health. Complementary Therapies in Clinical Practice 17(2): 113-5.
Sumathi T, Shobana C, Kumari BR, Nandhini, DN 2011. Protective role of Cynodon dactylon in ameliorating the aluminium-induced neurotoxicity in rat brain regions. Biological trace element research 144(1-3): 843-853.
Sumner MD, Elliott‑Eller M, Weidner G, Daubenmier JJ, Chew MH, Marlin R, Ornish D 2005. Effects of pomegranate juice consumption on myocardial perfusion in patients with coronary heart disease. American Journal of Cardiology, 96: 810‑814.
Vidal A, Fallarero A, Peña BR, Medina ME, Gra B, Rivera F, Vuorela PM 2003. Studies on the toxicity of Punica granatum L. (Punicaceae) whole fruit extracts. Journal of Ethnopharmacology 89: 295 300.

There are 34 citations in total.

Details

Primary Language	English
Journal Section	RESEARCH ARTICLE
Authors	Harun Çiftçi 0000-0002-3210-5566 Çiğdem Er Çalışkan 0000-0001-5821-7489 Fatih Kar 0000-0001-8356-9806 Ahmet Özkaya 0000-0002-0173-3084 Kenan Güçlü 0000-0002-0092-652X
Publication Date	April 30, 2022
Submission Date	March 14, 2021
Acceptance Date	May 7, 2021
Published in Issue	Year 2022 Volume: 25 Issue: 2

Cite

APA	Çiftçi, H., Er Çalışkan, Ç., Kar, F., … Özkaya, A. (2022). Evaluation of The Effects of Pomegranate Juice on Hepato-Nephrotoxicity in Male Rats Exposed to Aluminum. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(2), 215-222. https://doi.org/10.18016/ksutarimdoga.vi.896611