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Effects of Shilajit Added to Honeybee Sperm Extender on Sperm Freezing

Year 2022, Volume: 25 Issue: Ek Sayı 2, 591 - 596, 30.12.2022

Arda Onur Özkök , Burcu Yalçın Murat Selcuk

https://doi.org/10.18016/ksutarimdoga.vi.1031295
Cited By: 1

Abstract

The aim of this study was to determine the effects of shilajit (S) at different doses (0, 5, 10, 15, 20%) added to honey bee (Apis melilifera) semen extender on motility, plasma membrane integrity and acrosome integrity parameters. In the study, a total of 25 colonies, 5 groups and 5 colonies in each group, were used. The control group (S-0) constitutes the group where shilajit was not added to the honeybee extender. S-1, S-2, S-3 and S-4 groups were formed as groups with 5, 10, 15 and 20% shilajit added to the honey bee extender, respectively. Sperm were collected from approximately 400 drones aged 14-21 days in approximately 25 colonies that were fed every other day. Then they were frozen in liquid nitrogen vapor and stored in liquid nitrogen at -196°C for approximately 7 months. At the end of this period, the frozen samples were thawed at 37°C and examined in terms of relevant parameters. Compared with other groups, S-3 and S-4 groups increased sperm motility (p<0.05); It was determined that S-2, S-3 and S-4 groups increased plasma membrane integrity while the acrosome integrity was significantly preserved in the groups with different doses of shilajit added to the diluent compared to the control group (p<0.01). As a result, it was concluded that different doses of shilajit to honey bee extender had positive effects on spermatological parameters.

Keywords

Honeybee Freezing Shilajit Sperma

References

  • Agarwal SP, Khanna R, Karmarkar R, Anwer MK, Khar RK 2007. Shilajit: A Review. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 21(5): 401-405.
  • Alcay S, Cakmak S, Cakmak, Mulkpinar E, Toker MB, Ustuner B, Şen H Nur Z 2019. Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. Kafkas Univ Vet Fak Derg 25(4):553-557.
  • Bhattacharya SK, Sen AP, Ghosal S 1995. Effects of Shilajit on Biogenic Free Radicals. Phytotherapy Research 9(1): 56-59.
  • Biswas TK, Pandit S, Mondal S, Biswas SK, Jana U, Ghosh T, Tripathi PC, Debnath PK, Auddy RG, Auddy B 2010. Clinical Evaluation of Spermatogenic Activity of Processed Shilajit in Oligospermia. Andrologia 42(1): 48-56.
  • Burley LM, Fell RD, Saacke RG 2008. Survival of Honey Bee (Hymenoptera: Apidae) Spermatozoa Incubated at Room Temperature From Drones Exposed to Miticides. Journal of Economic Entomology 101(4): 1081-1087.
  • Collins AM 2000. Survival of Honey Bee (Hymenoptera: Apidae) Spermatozoa Stored at Above-Freezing Temperatures. Journal of Economic Entomology 93(3): 568-571.
  • Ghosal S 1990. Chemistry of Shilajit, an İmmunomodulatory Ayurvedic Rasayan. Pure and Applied Chemistry 62(7): 1285-1288.
  • Harbo JR 1979. Storage of Honeybee Spermatozoa at− 196° C. Journal of Apicultural Research 18(1): 57-63.
  • Harbo JR 1981. Viability of Honey Bee Eggs From Progeny of Frozen Spermatozoa. Annals of the Entomological Society of America 74(5): 482-486.
  • Hopkins BK, Herr C 2010. Factors Affecting the Successful Cryopreservation of Honey Bee (Apis Mellifera) Spermatozoa. Apidologie 41(5): 548-556.
  • Ikram-ul-Haq M, Ahmad M, Zubair M, Gul ST, Bashir MI 2016. Effects of Asphaltum (Shilajit) on Scrotal Circumference and Semen Quality Parameters of Adult Lohi Rams. Journal of Entomology and Zoology Studies 4(2): 559-563.
  • Kumar S, Kumar A, Singh AK, Honparkhe M, Singh P, Malhotra P. 2018. Improvement in Post-Thaw Semen Quality by Minimizing the Lipid Peroxidation Following Herbal Treatment in Sub Fertile Buffalo Bulls. The Pharma Innovation, 7(5, Part D): 240.
  • Locke SJ, Peng YS 1993. The Effects of Drone Age, Semen Storage and Contamination on Semen Quality in the Honey Bee (Apis Mellifera). Physiological Entomology 18(2): 144-148.
  • Mishra RK, Jain A, Singh SK 2018. Profertility Effects of Shilajit on Cadmium‐Induced Infertility in Male Mice. Andrologia 50(8): e13064.
  • Paillard M, Rousseau A, Giovenazzo P, Bailey JL 2017. Preservation of Domesticated Honey Bee (Hymenoptera: Apidae) Drone Semen. Journal of Economic Entomology 110(4): 1412-1418.
  • Rege A, Juvekar P, Juvekar A 2012. In Vitro Antioxidant and Anti-arthritic Activities of Shilajit. Int J Pharm Pharm Sci 4(2): 650-3.
  • Rousseau A, Giovenazzo P 2016. Optimizing Drone Fertility with Spring Nutritional Supplements to Honey Bee (Hymenoptera: Apidae) Colonies. Journal of Economic Entomology 109(3): 1009-1014.
  • Sultan J, Awan MA, Rakha BA, Waqar SA, Ansari MS, Naz S, Iqbal S, Akhter S 2021. Asphaltum Improves the Post-thaw Quality and Antioxidant Status of Nili Ravi Buffalo Bull Sperm. Biopreservation and Biobanking 19(3): 194-203.
  • Surapaneni DK, Adapa SRSS, Preeti K, Teja GR, Veeraragavan M, Krishnamurthy S 2012. Shilajit Attenuates Behavioral Symptoms of Chronic Fatigue Syndrome by Modulating the Hypothalamic–Pituitary–Adrenal Axis and Mitochondrial Bioenergetics in Rats. Journal of Ethnopharmacology 143(1): 91-99.
  • Taylor MA, Guzmán-Novoa E, Morfin N, Buhr MM 2009. Improving Viability of Cryopreserved Honey Bee (Apis Mellifera L.) Sperm with Selected Diluents, Cryoprotectants, and Semen Dilution Ratios. Theriogenology 72(2): 149-159.
  • Tripathi YB, Shukla S, Chaurasia S, Chaturvedi S 1996. Antilipid Peroxidative Property of Shilajit. Phytotherapy Research 10(3): 269-270.
  • Xiao Y, Wu Z, Wang M 2018. Effects of Fulvic Acids on Goat Sperm. Zygote 26(3): 220-223.
  • Zubair M 2016. Effects of Asphaltum (Shilajit) on Male Reproductive System (Mini Review). Sexual Dysfunction 3: 1.

Bal Arısı Sperma Sulandırıcısına Katılan Shilajitin Spermanın Dondurulması Üzerine Etkileri

Year 2022, Volume: 25 Issue: Ek Sayı 2, 591 - 596, 30.12.2022

Arda Onur Özkök , Burcu Yalçın Murat Selcuk

https://doi.org/10.18016/ksutarimdoga.vi.1031295
Cited By: 1

Abstract

Bu çalışmanın amacı, bal arısı (Apis melifera) sperma sulandırıcısına katılan farklı dozlardaki (%0, 5, 10, 15, 20) shilajitin (S) motilite, plazma membran bütünlüğü ve akrozom bütünlüğü parametreleri üzerine etkilerini belirlemektir. Çalışmada, 5 grup ve her bir grupta 5’er adet koloni olmak üzere toplam 25 adet koloni kullanıldı. Kontrol grubu (S-0), bal arısı sulandırıcısına shilajit ilavesi yapılmayan grubu oluşturmaktadır. S-1, S-2, S-3 ve S-4 grupları ise bal arısı sulandırıcısına sırasıyla %5, 10, 15 ve 20 shilajit ilaveli gruplar olarak oluşturuldu. Gün aşırı beslenen yaklaşık 25 adet koloni içerisinde 14-21 günlük yaşta bulunan yaklaşık 400 erkek arıdan sperma toplandı ve sıvı azot buharında dondurularak, -196°C sıvı azot içerisinde yaklaşık 7 ay süre ile saklandı. Bu süre sonunda dondurulan numuneler 37°C’ de çözdürülerek ilgili parametreler yönünden incelendi. Diğer gruplar ile karşılaştırıldığında, S-3 ve S-4 gruplarının sperma motilite değerini arttırdığı (p<0.05); S-2, S-3 ve S-4 gruplarının plazma membran bütünlüğünü arttırdığı tespit edildi. Sulandırıcıya farklı dozlarda shilajit ilave edilen gruplarda, kontrol grubuna kıyasla akrozom bütünlüğününün önemli derecede korunduğu belirlendi (p<0.01). Sonuç olarak, bal arısı sulandırıcısına farklı dozlardaki shilajitin spermatolojik parametreler üzerine olumlu etkileri olduğu saptandı.

Keywords

Bal arısı Dondurma Shilajit Sperma

References

  • Agarwal SP, Khanna R, Karmarkar R, Anwer MK, Khar RK 2007. Shilajit: A Review. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives 21(5): 401-405.
  • Alcay S, Cakmak S, Cakmak, Mulkpinar E, Toker MB, Ustuner B, Şen H Nur Z 2019. Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. Kafkas Univ Vet Fak Derg 25(4):553-557.
  • Bhattacharya SK, Sen AP, Ghosal S 1995. Effects of Shilajit on Biogenic Free Radicals. Phytotherapy Research 9(1): 56-59.
  • Biswas TK, Pandit S, Mondal S, Biswas SK, Jana U, Ghosh T, Tripathi PC, Debnath PK, Auddy RG, Auddy B 2010. Clinical Evaluation of Spermatogenic Activity of Processed Shilajit in Oligospermia. Andrologia 42(1): 48-56.
  • Burley LM, Fell RD, Saacke RG 2008. Survival of Honey Bee (Hymenoptera: Apidae) Spermatozoa Incubated at Room Temperature From Drones Exposed to Miticides. Journal of Economic Entomology 101(4): 1081-1087.
  • Collins AM 2000. Survival of Honey Bee (Hymenoptera: Apidae) Spermatozoa Stored at Above-Freezing Temperatures. Journal of Economic Entomology 93(3): 568-571.
  • Ghosal S 1990. Chemistry of Shilajit, an İmmunomodulatory Ayurvedic Rasayan. Pure and Applied Chemistry 62(7): 1285-1288.
  • Harbo JR 1979. Storage of Honeybee Spermatozoa at− 196° C. Journal of Apicultural Research 18(1): 57-63.
  • Harbo JR 1981. Viability of Honey Bee Eggs From Progeny of Frozen Spermatozoa. Annals of the Entomological Society of America 74(5): 482-486.
  • Hopkins BK, Herr C 2010. Factors Affecting the Successful Cryopreservation of Honey Bee (Apis Mellifera) Spermatozoa. Apidologie 41(5): 548-556.
  • Ikram-ul-Haq M, Ahmad M, Zubair M, Gul ST, Bashir MI 2016. Effects of Asphaltum (Shilajit) on Scrotal Circumference and Semen Quality Parameters of Adult Lohi Rams. Journal of Entomology and Zoology Studies 4(2): 559-563.
  • Kumar S, Kumar A, Singh AK, Honparkhe M, Singh P, Malhotra P. 2018. Improvement in Post-Thaw Semen Quality by Minimizing the Lipid Peroxidation Following Herbal Treatment in Sub Fertile Buffalo Bulls. The Pharma Innovation, 7(5, Part D): 240.
  • Locke SJ, Peng YS 1993. The Effects of Drone Age, Semen Storage and Contamination on Semen Quality in the Honey Bee (Apis Mellifera). Physiological Entomology 18(2): 144-148.
  • Mishra RK, Jain A, Singh SK 2018. Profertility Effects of Shilajit on Cadmium‐Induced Infertility in Male Mice. Andrologia 50(8): e13064.
  • Paillard M, Rousseau A, Giovenazzo P, Bailey JL 2017. Preservation of Domesticated Honey Bee (Hymenoptera: Apidae) Drone Semen. Journal of Economic Entomology 110(4): 1412-1418.
  • Rege A, Juvekar P, Juvekar A 2012. In Vitro Antioxidant and Anti-arthritic Activities of Shilajit. Int J Pharm Pharm Sci 4(2): 650-3.
  • Rousseau A, Giovenazzo P 2016. Optimizing Drone Fertility with Spring Nutritional Supplements to Honey Bee (Hymenoptera: Apidae) Colonies. Journal of Economic Entomology 109(3): 1009-1014.
  • Sultan J, Awan MA, Rakha BA, Waqar SA, Ansari MS, Naz S, Iqbal S, Akhter S 2021. Asphaltum Improves the Post-thaw Quality and Antioxidant Status of Nili Ravi Buffalo Bull Sperm. Biopreservation and Biobanking 19(3): 194-203.
  • Surapaneni DK, Adapa SRSS, Preeti K, Teja GR, Veeraragavan M, Krishnamurthy S 2012. Shilajit Attenuates Behavioral Symptoms of Chronic Fatigue Syndrome by Modulating the Hypothalamic–Pituitary–Adrenal Axis and Mitochondrial Bioenergetics in Rats. Journal of Ethnopharmacology 143(1): 91-99.
  • Taylor MA, Guzmán-Novoa E, Morfin N, Buhr MM 2009. Improving Viability of Cryopreserved Honey Bee (Apis Mellifera L.) Sperm with Selected Diluents, Cryoprotectants, and Semen Dilution Ratios. Theriogenology 72(2): 149-159.
  • Tripathi YB, Shukla S, Chaurasia S, Chaturvedi S 1996. Antilipid Peroxidative Property of Shilajit. Phytotherapy Research 10(3): 269-270.
  • Xiao Y, Wu Z, Wang M 2018. Effects of Fulvic Acids on Goat Sperm. Zygote 26(3): 220-223.
  • Zubair M 2016. Effects of Asphaltum (Shilajit) on Male Reproductive System (Mini Review). Sexual Dysfunction 3: 1.
There are 23 citations in total.

Details

Primary Language Turkish
Subjects Agricultural, Veterinary and Food Sciences
Journal Section RESEARCH ARTICLE
Authors

Arda Onur Özkök 0000-0001-9932-3608

Burcu Yalçın 0000-0002-5728-1478

Murat Selcuk 0000-0003-1371-6297

Publication Date December 30, 2022
Submission Date December 1, 2021
Acceptance Date April 1, 2022
Published in Issue Year 2022 Volume: 25 Issue: Ek Sayı 2

Cite

APA Özkök, A. O., Yalçın, B., & Selcuk, M. (2022). Bal Arısı Sperma Sulandırıcısına Katılan Shilajitin Spermanın Dondurulması Üzerine Etkileri. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 2), 591-596. https://doi.org/10.18016/ksutarimdoga.vi.1031295

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