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Investigation (In-Vitro) of Antiproliferative Properties of Pseudopediastrum boryanum (Turpin) E. Hegewald Extracts in Various Cancer Cell Lines

Year 2024, Volume: 27 Issue: 5, 1026 - 1031, 17.09.2024

Tuğba Demiriz Yücer

https://doi.org/10.18016/ksutarimdoga.vi.1420650

Abstract

In this study, the antiproliferative effects of Pseudopediastrum boryanum isolated and cultured from Tokat Yeşilırmak River benthic habitats were investigated with in-vitro experiments. The Anticancer activity of algae extracts was tested in cancer cell lines using the BrdU cell proliferation ELISA method. Vero (African Green Monkey Kidney Tumour Cells) and HeLa (Human Uterine Carcinoma Cells) were examined in concentrations of 100, 250 and 500 µg mL-1. As a result of antiproliferative tests, P. boryanum was found to have effective anti-cancer properties. P. boryanum extract concentrations of 250 and 500 µg mL-1 had a significant cytotoxic effect on Vero and HeLa cell lines after 24 hours. According to the results, P. boryanum is estimated to be a good option for quantitative pharmaceutical analyses and could be evaluated in the pharmaceutical industry.

Keywords

Antiproliferative effect BrdU Chlorophyta Pseudopediastrum boryanum

Thanks

The author thanks Professor doctor Köksal Pabuçcu for his help in the diagnosis of algae species.

References

  • Alghazeer, R., Howell, N. K., El-Naili, M. B. & Awayn, N. (2018). Anticancer and Antioxidant Activities of Some Algae from Western Libyan Coast. Natural Science, 10(7), 232-246. doi: 10.4236/ns.2018.107025
  • Altuner, Z., Pabuçcu, K. & Türkekul, İ. (2002). Tohumsuz Bitkiler Sistematiği, Altan Yayınları, Ankara, pp. 1:87.
  • Andersen, R. A. (2004). Algal Culturing Techniques. Elsevier Academic Press, Phycological Society, New York, 356 pg.
  • Chon S.U., Kim Y.M., Park Y.J., Heo B.G., Park Y.S., & Gorinstein S. (2009). Antioxidant and antiproliferative effects of methanol extracts from raw and fermented parts of mulberry plant (Morus alba L.). European Food Research and Technology, 230,231–237. doi: 10.1007/s00217-009-1165-2
  • Coşkun, T., Çelekli, A., Solak, C.N., Ongun Sevindik, T., Demir, N., Soylu, E. N., Sömek, H., Çetin, T., Karaaslan,Y., Öterler, B., Maraşlıoğlu, F., Yilmaz, E., Tunca, H., Güzel, U., Koca, M. & Temizel, B. (2023). New Records for the Turkish Freshwater Algal Flora in Twenty Five River Basins of Türkiye, Part I: Bacillariophyta. KSU Journal of Agriculture and Nature, 26 (5), 991-1004. doi:10.18016/ ksutarimdoga.vi. 1058856
  • Dulbecco, R. & Freeman, G. (1959). Plaque production by the polyoma virus. Virology, 8(3), 396-397. doi: 10.1016/0042-6822(59)90043-1
  • Elmastas, M., Celik, S. M., Genc, N., Aksit, H., Erenler, R., & Gulcin, İ. (2018). Antioxidant activity of an anatolian herbal tea—Origanum minutiflorum: isolation and characterization of its secondary metabolites. International Journal of Food Properties, 21(1), 374-384. doi:10.1080/10942912. 2017.1416399
  • Erenler, R., Pabuçcu, K., Yağlıoğlu, A. S., Demirtaş, I., & Gül, F. (2016). Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 71(3-4), 87-92. doi:10.1515/znc-2016-0010.
  • Garcez, M. C. S., Ferreiraa, S. P., Dora, C. L., Horta, M. A., Giroldob, D., Prates, D. F., Radmannd, E. M., Bemvenutid, R. H., Costad, J. A. V., Furlongd, E. V. & Baisch, A. L. M. (2020). Phenolic compounds and antioxidant capacity of Pediastrum boryanum (Chlorococcales) biomass. International Journal of Environmental Health Research, 23, 1-13. doi: 10.1080/09603123. 2020.1744113
  • Guiry, M. D. & Guiry, G. M. (2021). AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. Retrieved in July, 21, from http://www.algaebase.org
  • Işıkdağ, İ., Özkay, Y. & İncesu, Z. (2011). Synthesis And Anticancer Activity of Some Bisquinoxaline Derivatives. Turkish Journal of Pharmaceutical Sciences, 8(2), 179-188
  • Lehner, B., Sandner, B., Marschallinger, J., Lehner, C., Furtner, T., Couillard-Despres, S., Rivera, F. & Brockhoff, G. (2011). The dark side of BrdU in neural stem cell biology: Detrimental effects on cell cycle, differentiation and survival. Cell and Tissue Research, 345(3), 313–28. doi: 10.1007/s00441-011-1213-7
  • Leliaert, F., De Clerck, O., Verbruggen, H., Boedeke, C. & Coppejan, E. (2007). Molecular phylogeny of the Siphonocladales (Chlorophyta: Cladophorophyceae). Molecular Phylogenetics and Evolution, 44(3), 1237–1256. doi: 10.1016/ j.ympev.2007.04.016
  • Lobban, C. S., Chapman, D. J. & Kremer, B. P. (1988). Experimental Phycology A Laboratory Manual. Chambridge Univ.Press, London.
  • Lıu, M., Lı, X. Q., Weber, C., Lee, C. Y., Brown, J. & Lıu, R. H. (2002). Antioxidant and Antiproliferative Activities -of Raspberries. Journal of Agricultural Food Chemistry, 50(10), 2926-2930. doi: 10.1021/jf0111209
  • Masterson, C. & O’Dea, S. (2007). 5-Bromo-2-deoxyuridine activates DNA damage signalling responses and induces a senescence-like phenotype in p16-null lung cancer cells. Preclinical report, 18(9),1053-1068. doi: 10.1097/CAD. 0b013e32825209f6
  • Mofeed, J., Deyab, M. A. & Abd El-Halim, E. H. (2018). Anticancer activity of some filamentous cyanobacterial isolates against Hep-G2 and MCF-7 cancer cell lines. International Journal of Life Sciences, 8(1), 10-17.
  • Mofeed, J. (2019). Stimulating Gamma-Linolenic acid productivity by Arthrospira platensis (Spirulina platensis) under different culture conditions (temperatures, light regime, and H2O2 stress). Egyptian Academic Journal of Biological Sciences, 11(1), 89-99. doi: 10.21608/EAJBSG.2019.85014
  • Mtolera, M. S. P. & Semesi, A. K. (1996). Antimicrobial activity of extracts from six green algae from Tanzania. Current Trends in Marine Botanical Research in East African Region, 211-217.
  • Murakami, M., Makabe, K., Yamaguchi, K. & Konosu, S. (1989). Cytotoxic polyunsaturated fatty acid from Pediastrum. Phytochemistry, 28(2),625-626. doi: 10.1016/0031-9422(89)80065-2
  • Norris, J. N. (2010). Marine Algae of the Northern Gulf of California: Chlorophyta and Phaeophyceae. Smithsonion Contributions to Botany, 94, 210-271. doi: 10.5479/si.0081024X.94.276
  • Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M. & Stanier, R. Y. (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microb., 111, 1-61.
  • Pabuçcu, K. & Demiriz Yücer, T. (2022). Antibacterial and Antifungal effects of extracts of Spirogyra aequinoctialis prepared with different solvents. KSU Journal of Agriculture and Nature, 25 (5), 927-932. doi:10.18016/ ksutarimdoga.vi. 979361
  • Sathasivam, R., Radhakrishnan, R., Hashem, A. & AbdAllah, E. F. (2017). Microalgae metabolites: A rich source for food and medicine. Saudi Journal of Biological Sciences, 26(4), 709-722. doi: 10.1016/j.sjbs.2017.11.003
  • Syahril, M. Z., Roshani, O., Hasyimah, R., Hafiz, M. S., Sharida, M. D. & Ahmed, H. Y. (2011). Screening of Anticancer Activities of Crude Extracts of Unicellular Green Algae (Chlorella Vulgaris) And Filamentous Blue Green Algae (Spirulina Platensis) On Selected Cancer Cell Lines. International Conference on Applied Sciences, 82-87.
  • Wang, H. M. D., Chen, C. C., Huynh, P. & Chang, J. S. (2015). Exploring the potential of using algae in cosmetics. Bioresource Technology, 184, 355-362. doi: 10.1016/j.biortech.2014.12.001
  • Yang, J., Meyers, K. J., Heıde, J. V. D. & Lıu, R. H. (2004). Varietal Differences in Phenolic Content and Antioxidant and Antiproliferative Activities of Onions. Journal of Agricultural and Food Chemistry, 52(22), 6787-6793. doi: 10.1021/jf0307144
  • Yousefi, M. K., Hashtroudi, M. S., Moradi, A. M. & Ghasempour, A. R. (2018). In vitro investigating of anticancer activity of focuxanthin from marine brown seaweed species. Global J Environ Sci Manage, 4(1), 81-90. doi:10.22034/2018.04.01.008

Pseudopediastrum boryanum (Turpin) E. Hegewald Ekstraktlarının Çeşitli Kanser Hücre Hatlarında Antiproliferatif Özelliklerinin (İn Vitro) Araştırılması

Year 2024, Volume: 27 Issue: 5, 1026 - 1031, 17.09.2024

Tuğba Demiriz Yücer

https://doi.org/10.18016/ksutarimdoga.vi.1420650

Abstract

Bu çalışmada, Tokat Yeşilırmak Nehri bentik habitatlarından izole edilip kültürü yapılan Pseudopediastrum boryanum’un antiproliferatif etkileri, invitro deneylerle araştırıldı. Alg ekstraktlarının antikanser aktivitesi, BrdU hücre proliferasyon ELİSA yöntemi kullanılarak kanser hücre hatlarında denendi. Vero (Afrika Yeşil Maymun Böbrek Tümör Hücreleri) ve HeLa (İnsan Servikal Kanser Hücreleri) üzerinde 100, 250 ve 500 µg mL-1’ lik konsantrasyonlarda incelendi. Antiproliferatif testler sonucunda, P. boryanum’un, etkili antikanser özelliğe sahip olduğu görüldü. P. boryanum’un 250 ve 500 µg mL-1’ lik ekstrakt konsantrasyonlarının 24 saat sonunda Vero ve HeLa hücre hatları üzerinde, önemli derecede sitotoksik etki gösterdiği kaydedildi. Elde edilen sonuçlara göre, P. boryanum'un kantitatif farmasötik analizler için iyi bir aday olduğu ve ilaç endüstrisinde değerlendirilebileceği tahmin edilmektedir.

Keywords

Antiproliferatif etki Chlorophyta Pseudopediastrum boryanum BrdU

References

  • Alghazeer, R., Howell, N. K., El-Naili, M. B. & Awayn, N. (2018). Anticancer and Antioxidant Activities of Some Algae from Western Libyan Coast. Natural Science, 10(7), 232-246. doi: 10.4236/ns.2018.107025
  • Altuner, Z., Pabuçcu, K. & Türkekul, İ. (2002). Tohumsuz Bitkiler Sistematiği, Altan Yayınları, Ankara, pp. 1:87.
  • Andersen, R. A. (2004). Algal Culturing Techniques. Elsevier Academic Press, Phycological Society, New York, 356 pg.
  • Chon S.U., Kim Y.M., Park Y.J., Heo B.G., Park Y.S., & Gorinstein S. (2009). Antioxidant and antiproliferative effects of methanol extracts from raw and fermented parts of mulberry plant (Morus alba L.). European Food Research and Technology, 230,231–237. doi: 10.1007/s00217-009-1165-2
  • Coşkun, T., Çelekli, A., Solak, C.N., Ongun Sevindik, T., Demir, N., Soylu, E. N., Sömek, H., Çetin, T., Karaaslan,Y., Öterler, B., Maraşlıoğlu, F., Yilmaz, E., Tunca, H., Güzel, U., Koca, M. & Temizel, B. (2023). New Records for the Turkish Freshwater Algal Flora in Twenty Five River Basins of Türkiye, Part I: Bacillariophyta. KSU Journal of Agriculture and Nature, 26 (5), 991-1004. doi:10.18016/ ksutarimdoga.vi. 1058856
  • Dulbecco, R. & Freeman, G. (1959). Plaque production by the polyoma virus. Virology, 8(3), 396-397. doi: 10.1016/0042-6822(59)90043-1
  • Elmastas, M., Celik, S. M., Genc, N., Aksit, H., Erenler, R., & Gulcin, İ. (2018). Antioxidant activity of an anatolian herbal tea—Origanum minutiflorum: isolation and characterization of its secondary metabolites. International Journal of Food Properties, 21(1), 374-384. doi:10.1080/10942912. 2017.1416399
  • Erenler, R., Pabuçcu, K., Yağlıoğlu, A. S., Demirtaş, I., & Gül, F. (2016). Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 71(3-4), 87-92. doi:10.1515/znc-2016-0010.
  • Garcez, M. C. S., Ferreiraa, S. P., Dora, C. L., Horta, M. A., Giroldob, D., Prates, D. F., Radmannd, E. M., Bemvenutid, R. H., Costad, J. A. V., Furlongd, E. V. & Baisch, A. L. M. (2020). Phenolic compounds and antioxidant capacity of Pediastrum boryanum (Chlorococcales) biomass. International Journal of Environmental Health Research, 23, 1-13. doi: 10.1080/09603123. 2020.1744113
  • Guiry, M. D. & Guiry, G. M. (2021). AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. Retrieved in July, 21, from http://www.algaebase.org
  • Işıkdağ, İ., Özkay, Y. & İncesu, Z. (2011). Synthesis And Anticancer Activity of Some Bisquinoxaline Derivatives. Turkish Journal of Pharmaceutical Sciences, 8(2), 179-188
  • Lehner, B., Sandner, B., Marschallinger, J., Lehner, C., Furtner, T., Couillard-Despres, S., Rivera, F. & Brockhoff, G. (2011). The dark side of BrdU in neural stem cell biology: Detrimental effects on cell cycle, differentiation and survival. Cell and Tissue Research, 345(3), 313–28. doi: 10.1007/s00441-011-1213-7
  • Leliaert, F., De Clerck, O., Verbruggen, H., Boedeke, C. & Coppejan, E. (2007). Molecular phylogeny of the Siphonocladales (Chlorophyta: Cladophorophyceae). Molecular Phylogenetics and Evolution, 44(3), 1237–1256. doi: 10.1016/ j.ympev.2007.04.016
  • Lobban, C. S., Chapman, D. J. & Kremer, B. P. (1988). Experimental Phycology A Laboratory Manual. Chambridge Univ.Press, London.
  • Lıu, M., Lı, X. Q., Weber, C., Lee, C. Y., Brown, J. & Lıu, R. H. (2002). Antioxidant and Antiproliferative Activities -of Raspberries. Journal of Agricultural Food Chemistry, 50(10), 2926-2930. doi: 10.1021/jf0111209
  • Masterson, C. & O’Dea, S. (2007). 5-Bromo-2-deoxyuridine activates DNA damage signalling responses and induces a senescence-like phenotype in p16-null lung cancer cells. Preclinical report, 18(9),1053-1068. doi: 10.1097/CAD. 0b013e32825209f6
  • Mofeed, J., Deyab, M. A. & Abd El-Halim, E. H. (2018). Anticancer activity of some filamentous cyanobacterial isolates against Hep-G2 and MCF-7 cancer cell lines. International Journal of Life Sciences, 8(1), 10-17.
  • Mofeed, J. (2019). Stimulating Gamma-Linolenic acid productivity by Arthrospira platensis (Spirulina platensis) under different culture conditions (temperatures, light regime, and H2O2 stress). Egyptian Academic Journal of Biological Sciences, 11(1), 89-99. doi: 10.21608/EAJBSG.2019.85014
  • Mtolera, M. S. P. & Semesi, A. K. (1996). Antimicrobial activity of extracts from six green algae from Tanzania. Current Trends in Marine Botanical Research in East African Region, 211-217.
  • Murakami, M., Makabe, K., Yamaguchi, K. & Konosu, S. (1989). Cytotoxic polyunsaturated fatty acid from Pediastrum. Phytochemistry, 28(2),625-626. doi: 10.1016/0031-9422(89)80065-2
  • Norris, J. N. (2010). Marine Algae of the Northern Gulf of California: Chlorophyta and Phaeophyceae. Smithsonion Contributions to Botany, 94, 210-271. doi: 10.5479/si.0081024X.94.276
  • Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M. & Stanier, R. Y. (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microb., 111, 1-61.
  • Pabuçcu, K. & Demiriz Yücer, T. (2022). Antibacterial and Antifungal effects of extracts of Spirogyra aequinoctialis prepared with different solvents. KSU Journal of Agriculture and Nature, 25 (5), 927-932. doi:10.18016/ ksutarimdoga.vi. 979361
  • Sathasivam, R., Radhakrishnan, R., Hashem, A. & AbdAllah, E. F. (2017). Microalgae metabolites: A rich source for food and medicine. Saudi Journal of Biological Sciences, 26(4), 709-722. doi: 10.1016/j.sjbs.2017.11.003
  • Syahril, M. Z., Roshani, O., Hasyimah, R., Hafiz, M. S., Sharida, M. D. & Ahmed, H. Y. (2011). Screening of Anticancer Activities of Crude Extracts of Unicellular Green Algae (Chlorella Vulgaris) And Filamentous Blue Green Algae (Spirulina Platensis) On Selected Cancer Cell Lines. International Conference on Applied Sciences, 82-87.
  • Wang, H. M. D., Chen, C. C., Huynh, P. & Chang, J. S. (2015). Exploring the potential of using algae in cosmetics. Bioresource Technology, 184, 355-362. doi: 10.1016/j.biortech.2014.12.001
  • Yang, J., Meyers, K. J., Heıde, J. V. D. & Lıu, R. H. (2004). Varietal Differences in Phenolic Content and Antioxidant and Antiproliferative Activities of Onions. Journal of Agricultural and Food Chemistry, 52(22), 6787-6793. doi: 10.1021/jf0307144
  • Yousefi, M. K., Hashtroudi, M. S., Moradi, A. M. & Ghasempour, A. R. (2018). In vitro investigating of anticancer activity of focuxanthin from marine brown seaweed species. Global J Environ Sci Manage, 4(1), 81-90. doi:10.22034/2018.04.01.008
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Details

Primary Language English
Subjects Microbiology (Other)
Journal Section RESEARCH ARTICLE
Authors

Tuğba Demiriz Yücer 0000-0002-2494-4511

Early Pub Date July 2, 2024
Publication Date September 17, 2024
Submission Date January 16, 2024
Acceptance Date March 28, 2024
Published in Issue Year 2024 Volume: 27 Issue: 5

Cite

APA Demiriz Yücer, T. (2024). Investigation (In-Vitro) of Antiproliferative Properties of Pseudopediastrum boryanum (Turpin) E. Hegewald Extracts in Various Cancer Cell Lines. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(5), 1026-1031. https://doi.org/10.18016/ksutarimdoga.vi.1420650
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