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Evaluation Of Raftlin And 8-Iso Prostaglandin Levels On The Clinical Severity Of Cases With Covid-19 In Turkey : A Cross-Sectional Study

Year 2024, Volume: 27 Issue: Ek Sayı 2 (Suppl 2), 298 - 306, 31.12.2024

Ergul Belge Kurutas , Erkan Öner , Figen Güzelgül

https://doi.org/10.18016/ksutarimdoga.vi.1481677

Abstract

COVID-19 is an infectious respiratory disease caused by the severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2). Raftlin is a large lipid raft protein found in cell membranes and plays a crucial role in inducing autoimmune and vascular inflammatory responses. Although there have been few studies on 8-iso-prostaglandin F2α (8-iso-PGF2α) as a marker of oxidative stress in COVID-19, the levels of raftlin in the disease have not been previously investigated. The objective of in this study was to evaluate the levels of Raftlin and 8-iso-PGF2α in COVID-19 patients. We analyzed clinical findings from three groups: healthy controls, patients with a mild course of COVID-19, and patients with a severe course of COVID-19. We measured the levels of Raftlin and 8-iso-PGF2α in serum samples using ELISA. This results showed that the levels of Raftlin and 8-iso-PGF2α were higher in patients with severe COVID-19 compared to the control and mild course groups. Furthermore, a significant positive correlation was found between Raftlin and 8-iso-PGF2α in the severe COVID-19 group. Raftlin was identified as a potentially important biomarker in COVID-19 patients. This study is valuable as it is the first to investigate changes in Raftlin and 8-iso-PGF2α levels in COVID-19 disease.

Keywords

COVID-19 Raftlin 8-iso-prostaglandin F2α

References

  • Angel, B.P.L., Jadoon, S., Malik, A. 2018. A comprehensive review article on isoprostanes as biological markers. Biochemical Pharmacology 7, 2.
  • Bae, J.S., Yang, L., & Rezaie, A.R. 2008. Lipid raft localization regulates the cleavage specifactivated protease-activated receptor 1 in endothelial cells. Journal of Thrombosis and Haemostasis 6, 954–961.
  • Bae, J.S., Yang, L., Rezaie, A.R. 2007. Receptors of the protein C activation and activated protein C signalling pathways are colocalised in lipid rafts of endothelial cells. Proceedings of the National Academy of Sciences 2004, 2867–2872.
  • Bilal, N., Kurutas, E.B., Orhan, I., Bilal, B., Doganer, A. 2021. Evaluation of preoperative and postoperative serum interleukin-6, interleukin-8, tumour necrosis factor α and raftlin levels in patients with obstructive sleep apnea. Sleep and Breathing 25, 819-826.
  • Bilgen, F., Ural, A., Kurutas, E.B., Bekerecioglu, M. 2019. The effect of oxidative stress and raftlin levels on wound healing. International Wound Journal 16, 1178-1184.
  • Demirhan, I., Oner, E., Yuksel, Z., Yuksel, M., Kurutas, E.B. 2023. Raftlin and 8-iso-prostaglandin F2α levels and gene network analysis in patients with Modic changes. European Spine Journal 32, 2368–2376.
  • Derouiche, S. 2020. Oxidative stress associated with SARS-Cov-2 (COVID-19) increases the severity of the lung disease—a systematic review. Journal of Infectiology and Epidemiology 6, (3), 1–6.
  • Hui, D.S., Azhar, I.E., Madani, T.A., et al. 2020. The continuing 2019-nCoV epidemic threat of novel coronaviruses to global health - The latest 2019 novel coronavirus outbreak in Wuhan, China. International Journal of Infectious Diseases 91, 264-266.
  • Guan, W.J., Ni, Z.Y., Hu, Y., et al. 2020. China Medical Treatment Expert Group for Covid-19. Clinical Characteristics of Coronavirus Disease 2019 in China. The New England Journal of Medicine 30, 382(18), 1708-1720.
  • Juan, C.A., Pérez, J.M., Plou, F.J., Pérez-Lebeña, E. 2021. The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. International Journal of Molecular Sciences 22, 4642.
  • Karahan, M.D. 2021. Determination of CMV Viral Load Cut-off Value in Plasma in the Diagnosis of Cytomegalovirus Gastrointestinal Disease, Evaluation of Diagnostic Performance Measures of the Test and Developing a Scoring System to Predict the Disease. Hacettepe University Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Medical Specialty Thesis, Ankara.
  • Kaviarasan, S., Muniandy, S., Qvist, R., Ikram S.I., 2009. F2-Isoprostanes as Novel Biomarkers for Type 2 Diabetes: a Review. J Clin Biochem Nutr, 45, 1–8
  • Kurutas, E.B. 2016. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition Journal 5, 71.
  • Lee, W., Yoo, H., Ku, S., Kim, S., Bae, J. 2014. Raftlin: a New Biomarker in Human Sepsis. Inflammation 37, 706-711.
  • Milne, G.L., Yin, H., Morrow, J.D. 2008. Human biochemistry of the isoprostane pathway. J. Biol. Chem, 283,15533-7.
  • Miraloglu, M., Kurutas, E.B., Ozturk, P., Arican, O. 2016. Evaluation of local trace element status and 8-iso-prostaglandin F2α concentrations in patients with Tinea pedis. Biol Proced Online 18, 1.
  • Muhammad, Y., Aminu, Y.K., Ahmad, A.E., et al. 2020. An elevated 8-isoprostaglandin F2 alpha (8-iso-PGF2α) in COVID-19 subjects co-infected with malaria. The Pan African Medical Journal 21, 37,78.
  • Muhammad, Y., Kani, Y.A., Iliya, S., et al. 2021. Deficiency of antioxidants and increased oxidative stress in COVID-19 patients: A cross-sectional comparative study in Jigawa, Northwestern Nigeria. SAGE Open Medicine 9, 2050312121991246.
  • Noonong, K., Chatatikun, M., Surinkaew, S., et al. 2023. Mitochondrial oxidative stress, mitochondrial ROS storms in long COVID pathogenesis. Frontiers in Immunology 14, 1275001.
  • Nour, E.E.M., El-Readi, M.Z. 2020. Evaluation of the diagnostic and predicative values of 8-iso-prostaglandin F2α as a biomarker of breast cancer. Oncology Research and Treatment 43, (10), 506–517.
  • Pike, L.J. 2003. Lipid rafts: bringing order to chaos. Journal of Lipid Research 44, 655–667.
  • Pratico, D., Rokach, J., Lawson, J., FitzGerald, G.A., 2004. F2-isoprostanes as indices of lipid peroxidation in inflammatory diseases. Chem Phys Lipids, 128, 165-71.
  • Saeki, K., Miura, Y., Aki, D., Kurosaki, T., Yoshimura, A. 2003. The B cell-specific major raft protein, Raftlin, is necessary for the integrity of lipid raft and BCR signal transduction. EMBO Journal 22, 3015-3026.
  • Saeki, K., Fukuyama, S., Ayada, T., et al. 2009. A major lipid raft protein raftlin modulates T cell receptor signaling and enhances th17-mediated autoimmune responses. The Journal of Immunology 182, 5929–5937.
  • Schmidt, C., Kim, D., Ippolito, G.C., et al. 2009. Signalling of the BCR is regulated by a lipid rafts-localised transcription factor, Bright. EMBO Journal 28, 711-724.
  • Taylor, R. 1990. Interpretation of the correlation coefficient: a basic review. Journal of Diagnostic Medical Sonography 6, 35–39.
  • Tekin, M., Çalıskan, M.N., Kayak, D., Konca, C. 2017. The C-Reactive Protein a reliable marker for the diagnosis of bacterial infection. Health Sciences Journal of Adıyaman University 3, (2), 475-486.
  • The Novel Coronavirus Pneumonia Emergency Response Epidemiology Team. 2020. The epidemiological characteristics of an outbreak of 2019 novel coronavirus diseases (COVID-19)-China, 2020. China CDC Wkly. 2, (8), 113-122.
  • Watanabe, A., Tatematsu, M., Saeki, K., et al. 2011. Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation. Journal of Biological Chemistry 286, 10702–10711.
  • Žarković, N., Łuczaj, W., Jarocka-Karpowicz, I., et al. 2022. Diversified Effects of COVID-19 as a Consequence of the Differential Metabolism of Phospholipids and Lipid Peroxidation Evaluated in the Plasma of Survivors and Deceased Patients upon Admission to the Hospital. International Journal of Molecular Sciences 5, 23(19), 11810.

Türkiye'de Covid-19 Olgularında Raftlin ve 8-Iso Prostaglandin Düzeylerinin Klinik Şiddet Üzerine Etkisinin Değerlendirilmesi: Kesitsel Bir Çalışma

Year 2024, Volume: 27 Issue: Ek Sayı 2 (Suppl 2), 298 - 306, 31.12.2024

Ergul Belge Kurutas , Erkan Öner , Figen Güzelgül

https://doi.org/10.18016/ksutarimdoga.vi.1481677

Abstract

COVID-19, şiddetli akut solunum sendromu SARS-CoV-2‘nin neden olduğu bulaşıcı bir solunum yolu hastalığıdır. Raftlin, hücre zarlarında bulunan büyük bir lipid sal proteinidir ve otoimmün ve vasküler inflamatuar yanıtların indüklenmesinde önemli rol oynar. COVID-19'da oksidatif stresin bir belirteci olarak 8-iso-prostaglandin F2α (8-iso-PGF2α) ile ilgili az sayıda çalışma olmasına rağmen, hastalıktaki raftlin seviyeleri daha önce araştırılmamıştır. Çalışmamızın amacı, COVID-19 hastalarında Raftlin ve 8-iso-PGF2α düzeylerini değerlendirmektir. Üç gruptan elde edilen klinik bulguları analiz edildi: sağlıklı kontroller, hafif COVID-19 seyri olan hastalar ve şiddetli COVID-19 seyri olan hastalar. ELISA kit kullanarak serum örneklerinde Raftlin ve 8-iso-PGF2α seviyelerini ölçtük. Sonuçlarımız, Raftlin ve 8-iso-PGF2α seviyelerinin şiddetli COVID-19 hastalarında kontrol ve hafif seyirli gruplara kıyasla daha yüksek olduğunu göstermiştir. Ayrıca, şiddetli COVID-19 grubunda Raftlin ve 8-iso-PGF2α arasında anlamlı bir pozitif korelasyon bulunmuştur.
Raftlin, COVID-19 hastalarında potansiyel olarak önemli bir biyobelirteç olarak tanımlanmıştır. Bu çalışma, COVID-19 hastalığında Raftlin ve 8-iso-PGF2α seviyelerindeki değişiklikleri araştıran ilk çalışma olması açısından değerlidir.

Keywords

COVID-19 Raftlin 8-izo-prostaglandin

References

  • Angel, B.P.L., Jadoon, S., Malik, A. 2018. A comprehensive review article on isoprostanes as biological markers. Biochemical Pharmacology 7, 2.
  • Bae, J.S., Yang, L., & Rezaie, A.R. 2008. Lipid raft localization regulates the cleavage specifactivated protease-activated receptor 1 in endothelial cells. Journal of Thrombosis and Haemostasis 6, 954–961.
  • Bae, J.S., Yang, L., Rezaie, A.R. 2007. Receptors of the protein C activation and activated protein C signalling pathways are colocalised in lipid rafts of endothelial cells. Proceedings of the National Academy of Sciences 2004, 2867–2872.
  • Bilal, N., Kurutas, E.B., Orhan, I., Bilal, B., Doganer, A. 2021. Evaluation of preoperative and postoperative serum interleukin-6, interleukin-8, tumour necrosis factor α and raftlin levels in patients with obstructive sleep apnea. Sleep and Breathing 25, 819-826.
  • Bilgen, F., Ural, A., Kurutas, E.B., Bekerecioglu, M. 2019. The effect of oxidative stress and raftlin levels on wound healing. International Wound Journal 16, 1178-1184.
  • Demirhan, I., Oner, E., Yuksel, Z., Yuksel, M., Kurutas, E.B. 2023. Raftlin and 8-iso-prostaglandin F2α levels and gene network analysis in patients with Modic changes. European Spine Journal 32, 2368–2376.
  • Derouiche, S. 2020. Oxidative stress associated with SARS-Cov-2 (COVID-19) increases the severity of the lung disease—a systematic review. Journal of Infectiology and Epidemiology 6, (3), 1–6.
  • Hui, D.S., Azhar, I.E., Madani, T.A., et al. 2020. The continuing 2019-nCoV epidemic threat of novel coronaviruses to global health - The latest 2019 novel coronavirus outbreak in Wuhan, China. International Journal of Infectious Diseases 91, 264-266.
  • Guan, W.J., Ni, Z.Y., Hu, Y., et al. 2020. China Medical Treatment Expert Group for Covid-19. Clinical Characteristics of Coronavirus Disease 2019 in China. The New England Journal of Medicine 30, 382(18), 1708-1720.
  • Juan, C.A., Pérez, J.M., Plou, F.J., Pérez-Lebeña, E. 2021. The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. International Journal of Molecular Sciences 22, 4642.
  • Karahan, M.D. 2021. Determination of CMV Viral Load Cut-off Value in Plasma in the Diagnosis of Cytomegalovirus Gastrointestinal Disease, Evaluation of Diagnostic Performance Measures of the Test and Developing a Scoring System to Predict the Disease. Hacettepe University Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Medical Specialty Thesis, Ankara.
  • Kaviarasan, S., Muniandy, S., Qvist, R., Ikram S.I., 2009. F2-Isoprostanes as Novel Biomarkers for Type 2 Diabetes: a Review. J Clin Biochem Nutr, 45, 1–8
  • Kurutas, E.B. 2016. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition Journal 5, 71.
  • Lee, W., Yoo, H., Ku, S., Kim, S., Bae, J. 2014. Raftlin: a New Biomarker in Human Sepsis. Inflammation 37, 706-711.
  • Milne, G.L., Yin, H., Morrow, J.D. 2008. Human biochemistry of the isoprostane pathway. J. Biol. Chem, 283,15533-7.
  • Miraloglu, M., Kurutas, E.B., Ozturk, P., Arican, O. 2016. Evaluation of local trace element status and 8-iso-prostaglandin F2α concentrations in patients with Tinea pedis. Biol Proced Online 18, 1.
  • Muhammad, Y., Aminu, Y.K., Ahmad, A.E., et al. 2020. An elevated 8-isoprostaglandin F2 alpha (8-iso-PGF2α) in COVID-19 subjects co-infected with malaria. The Pan African Medical Journal 21, 37,78.
  • Muhammad, Y., Kani, Y.A., Iliya, S., et al. 2021. Deficiency of antioxidants and increased oxidative stress in COVID-19 patients: A cross-sectional comparative study in Jigawa, Northwestern Nigeria. SAGE Open Medicine 9, 2050312121991246.
  • Noonong, K., Chatatikun, M., Surinkaew, S., et al. 2023. Mitochondrial oxidative stress, mitochondrial ROS storms in long COVID pathogenesis. Frontiers in Immunology 14, 1275001.
  • Nour, E.E.M., El-Readi, M.Z. 2020. Evaluation of the diagnostic and predicative values of 8-iso-prostaglandin F2α as a biomarker of breast cancer. Oncology Research and Treatment 43, (10), 506–517.
  • Pike, L.J. 2003. Lipid rafts: bringing order to chaos. Journal of Lipid Research 44, 655–667.
  • Pratico, D., Rokach, J., Lawson, J., FitzGerald, G.A., 2004. F2-isoprostanes as indices of lipid peroxidation in inflammatory diseases. Chem Phys Lipids, 128, 165-71.
  • Saeki, K., Miura, Y., Aki, D., Kurosaki, T., Yoshimura, A. 2003. The B cell-specific major raft protein, Raftlin, is necessary for the integrity of lipid raft and BCR signal transduction. EMBO Journal 22, 3015-3026.
  • Saeki, K., Fukuyama, S., Ayada, T., et al. 2009. A major lipid raft protein raftlin modulates T cell receptor signaling and enhances th17-mediated autoimmune responses. The Journal of Immunology 182, 5929–5937.
  • Schmidt, C., Kim, D., Ippolito, G.C., et al. 2009. Signalling of the BCR is regulated by a lipid rafts-localised transcription factor, Bright. EMBO Journal 28, 711-724.
  • Taylor, R. 1990. Interpretation of the correlation coefficient: a basic review. Journal of Diagnostic Medical Sonography 6, 35–39.
  • Tekin, M., Çalıskan, M.N., Kayak, D., Konca, C. 2017. The C-Reactive Protein a reliable marker for the diagnosis of bacterial infection. Health Sciences Journal of Adıyaman University 3, (2), 475-486.
  • The Novel Coronavirus Pneumonia Emergency Response Epidemiology Team. 2020. The epidemiological characteristics of an outbreak of 2019 novel coronavirus diseases (COVID-19)-China, 2020. China CDC Wkly. 2, (8), 113-122.
  • Watanabe, A., Tatematsu, M., Saeki, K., et al. 2011. Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation. Journal of Biological Chemistry 286, 10702–10711.
  • Žarković, N., Łuczaj, W., Jarocka-Karpowicz, I., et al. 2022. Diversified Effects of COVID-19 as a Consequence of the Differential Metabolism of Phospholipids and Lipid Peroxidation Evaluated in the Plasma of Survivors and Deceased Patients upon Admission to the Hospital. International Journal of Molecular Sciences 5, 23(19), 11810.
There are 30 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other)
Journal Section RESEARCH ARTICLE
Authors

Ergul Belge Kurutas 0000-0002-6653-4801

Erkan Öner 0000-0002-6332-6484

Figen Güzelgül 0000-0002-2796-9511

Early Pub Date December 19, 2024
Publication Date December 31, 2024
Submission Date May 10, 2024
Acceptance Date August 31, 2024
Published in Issue Year 2024 Volume: 27 Issue: Ek Sayı 2 (Suppl 2)

Cite

APA Belge Kurutas, E., Öner, E., & Güzelgül, F. (2024). Evaluation Of Raftlin And 8-Iso Prostaglandin Levels On The Clinical Severity Of Cases With Covid-19 In Turkey : A Cross-Sectional Study. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(Ek Sayı 2 (Suppl 2), 298-306. https://doi.org/10.18016/ksutarimdoga.vi.1481677
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