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Evaluation of Cytotoxicity of Centaurea lycaonica Boiss. & Heldr. Plant on Human Cervical Cancer Cell Line with MTT Test and xCELLigence System

Year 2025, Volume: 28 Issue: 1, 47 - 52, 12.02.2025

Ayşe Kübra Karaboğa Arslan , Eylül Güngörenler , Leyla Paşayeva , Mehmet Bozkurt , Osman Tugay

https://doi.org/10.18016/ksutarimdoga.vi.1496499
Cited By: 1

Abstract

Many species belonging to the genus Centaurea L. are endemic in various parts of the world and are used in folk medicine. The cytotoxicity of some species belonging to this genus on cervical cancer cell lines was investigated. In this study, it was aimed to investigate the cytotoxic effect of dichloromethane (CRD) and methanol (CRM) extracts prepared by 24 h maceration method on human cervical cancer cell line (HeLa) at 48 h exposure based on the root part of Centaurea lycaonica, which is endemic to Turkey and about which there are almost no studies. The effect of different concentrations of plant extracts on cell viability was investigated using MTT and xCELLigence RTCA systems, which have different working principles, and IC50 values were determined. As a result, MTT findings of CRD and CRM extracts were consistent with xCELLigence analysis and found to have a cytotoxic effect on HeLa cells. It was thought that C. lycaonica species could be evaluated as a new natural source in cancer treatment. It is thought that this study will encourage the elucidation of the biological activities and mechanisms of action of many species endemic to Turkey.

Keywords

Centaurea lycaonica Cervical cancer HeLa MTT xCELLigence

Project Number

1919B012314005

References

  • Artun, F. T., & Karagöz, A. (2021). Antiproliferative and apoptosis-inducing effects of the methanolic extract of Centaurea hermannii in human cervical cancer cell line. Biotechnic & Histochemistry, 96(1), 1-10. https://doi.org/10.1080/10520295.2020.1751288
  • Atmaca, H., Bozkurt, E., Kısım, A., & Uslu, R. (2016). Comparative analysis of XTT assay and xCELLigence system by measuring cytotoxicity of resveratrol in human cancer cell lines. Turkish Journal of Biochemistry, 41(6), 413-421. https://doi.org/10.1515/tjb-2016-0128
  • Ayad, R., & Akkal, S. (2019). Phytochemistry and biological activities of Algerian Centaurea and related genera. Studies in natural products chemistry, 63, 357-414. https://doi.org/10.1016/B978-0-12-817901-7.00012-5
  • Brown, A. J., & Trimble, C. L. (2012). New technologies for cervical cancer screening. Best practice & research Clinical obstetrics & gynaecology, 26(2), 233-242. https://doi.org/10.1016/j.bpobgyn.2011.11.001
  • Davis P.H. (1970). Flora of Turkey and the East Aegean Islands. Vol. 3; 548-625.
  • Delgado-Waldo, I., Contreras-Romero, C., Salazar-Aguilar, S., Pessoa, J., Mitre-Aguilar, I., García-Castillo, V., ... & Jacobo-Herrera, N. J. (2023). A triple-drug combination induces apoptosis in cervical cancer-derived cell lines. Frontiers in Oncology, 13, 1106667. https://doi.org/10.3389/fonc.2023.1106667
  • Erol-Dayı, Ö., Pekmez, M., Bona, M., Aras Perk, A., Arda, N. (2011). Total phenolic contents, antioxidant activities cytotoxicity of three Centaurea species: C. calcitrapa subsp. calcitrapa, C. ptosimopappa C. spicata. Free Radicals and Antioxidants, 1(2), 31-36. https://doi.org/10.5530/ax.2011.2.7
  • Fattaheian-Dehkordi, S., Hojjatifard, R., Saeedi, M., & Khanavi, M. (2021). A review on antidiabetic activity of Centaurea spp.: A new approach for developing herbal remedies. Evidence-based complementary and alternative medicine, 2021. https://doi.org/10.1155/2021/5587938
  • Gras, A., Parada, M., Pellicer, J., Vallès, J., & Garnatje, T. (2022). Cancer and Traditional Plant Knowledge, an Interesting Field to Explore: Data from the Catalan Linguistic Area. Molecules, 27(13), 4070. https://doi.org/10.3390/molecules27134070
  • Huang, W., Gfeller, V., & Erb, M. (2018). Root volatiles in plant-plant interactions II: Root terpenes from Centaurea stoebe modify Taraxacum officinale root chemistry and root herbivore growth. bioRxiv, 441790. https://doi.org/10.1101/441790
  • Jabri, T., Imran, M., Aziz, A., Rao, K., Kawish, M., Irfan, M., ... & Shah, M. R. (2019). Design and synthesis of mixed micellar system for enhanced anticancer efficacy of Paclitaxel through its co-delivery with Naringin. Drug development and industrial pharmacy, 45(5), 703-714. https://doi.org/10.1080/03639045.2018.1550091
  • Keser, S., Keser, F., Turkoglu, İ., Kaygılı, O., ... & Turkoglu S. (2020). In Vitro Biological Evaluation and Phytochemical Contents of Three Centaurea L. Species Growing from Eastern Anatolia in Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(1), 148-156. https://doi.org/10.18016/ksutarimdoga.vi.589279
  • Khammar, A., & Djeddi, S. (2012). Pharmacological and biological properties of some Centaurea species. Eur J Sci Res, 84(3), 398-416. http://www.europeanjournalofscientificresearch.com
  • Kısa, D., Çelik, A., & İmamoğlu, R. (2024). Assessment of Inhibitory Ability Against Medicinally Important Enzymes with Invitro and In Silico Studies: Phenolic Content of Endemic Centaurea cadmea subsp. pontica. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(1), 14-25. https://doi.org/10.18016/ksutarimdoga.vi.1294720
  • Koc, S., Isgor, B. S., Isgor, Y. G., Shomali Moghaddam, N., & Yildirim, O. (2015). The potential medicinal value of plants from Asteraceae family with antioxidant defense enzymes as biological targets. Pharmaceutical biology, 53(5), 746-751. https://doi.org/10.3109/13880209.2014.942788
  • Lopes, D. C. D. X. P., de Oliveira, T. B., Viçosa, A. L., Valverde, S. S., & Júnior, E. R. (2021). Anti-inflammatory activity of the compositae family and its therapeutic potential. Planta Medica, 87(01/02), 71-100. https://doi.org/10.1055/a-1178-5158
  • Lu, X., Yang, F., Chen, D., Zhao, Q., Chen, D., Ping, H., & Xing, N. (2020). Quercetin reverses docetaxel resistance in prostate cancer via androgen receptor and PI3K/Akt signaling pathways. International Journal of Biological Sciences, 16(7), 1121. https://doi.org/10.7150/ijbs.41686
  • Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods, 65(1-2), 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  • Oberg, H. H., Peters, C., Kabelitz, D., & Wesch, D. (2020). Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro. In Methods in Enzymology (Vol. 631, pp. 429-441). Academic Press. https://doi.org/10.1016/bs.mie.2019.07.020
  • Rajaram, S., & Gupta, B. (2021). Screening for cervical cancer: Choices & dilemmas. Indian Journal of Medical Research, 154(2), 210-220. https://doi.org/10.4103/ijmr.IJMR_857_20
  • Saklani, A., & Kutty, S. K. (2008). Plant-derived compounds in clinical trials. Drug discovery today, 13(3-4), 161-171. https://doi.org/10.1016/j.drudis.2007.10.010
  • Shaikh, R., Daniel, A., & Lyng, F. M. (2023). Raman Spectroscopy for Early Detection of Cervical Cancer, a Global Women’s Health Issue-A Review. Molecules, 28(6), 2502. https://doi.org/10.3390/molecules28062502
  • Sung, H., Ferlay, J., Siegel, R. L., Laversanne, M., Soerjomataram, I., Jemal, A., & Bray, F. (2021). Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians, 71(3), 209-249. https://doi.org/10.3322/caac.21660
  • Wu, L., Cao, K., Ni, Z., Wang, S., Li, W., Liu, X., & Chen, Z. (2019). Rhein reverses doxorubicin resistance in SMMC‐7721 liver cancer cells by inhibiting energy metabolism and inducing mitochondrial permeability transition pore opening. Biofactors, 45(1), 85-96. https://doi.org/10.1002/biof.1462
  • Xu, J., Liu, D., Niu, H., Zhu, G., Xu, Y., Ye, D., ... & Zhang, Q. (2017). Resveratrol reverses Doxorubicin resistance by inhibiting epithelial-mesenchymal transition (EMT) through modulating PTEN/Akt signaling pathway in gastric cancer. Journal of Experimental & Clinical Cancer Research, 36, 1-14. https://doi.org/10.1186/s13046-016-0487-8
  • Yuan, C. H., Horng, C. T., Lee, C. F., Chiang, N. N., Tsai, F. J., Lu, C. C., ... & Chen, F. A. (2017). Epigallocatechin gallate sensitizes cisplatin‐resistant oral cancer CAR cell apoptosis and autophagy through stimulating AKT/STAT3 pathway and suppressing multidrug resistance 1 signaling. Environmental toxicology, 32(3), 845-855. https://doi.org/10.1002/tox.22284

Centaurea lycaonica Boiss. & Heldr. Bitkisinin İnsan Servikal Kanser Hücre Hattında Sitotoksisitesinin MTT Testi ve xCELLigence Sistemi ile Değerlendirilmesi

Year 2025, Volume: 28 Issue: 1, 47 - 52, 12.02.2025

Ayşe Kübra Karaboğa Arslan , Eylül Güngörenler , Leyla Paşayeva , Mehmet Bozkurt , Osman Tugay

https://doi.org/10.18016/ksutarimdoga.vi.1496499
Cited By: 1

Abstract

Centaurea L. cinsine ait birçok tür, dünyanın çeşitli bölgelerinde endemik olarak varlığını sürdürmekte ve halk hekimliğinde kullanımlarıyla literatürde yerini almaktadır. Söz konusu cinse ait bazı türlerin servikal kanser hücre hattı üzerindeki sitotoksisitesi incelenmiştir. Bu çalışmada Türkiye’ye endemik ve hakkında yok denecek kadar az sayıda çalışma bulunan Centaurea lycaonica türünün kök kısmından hareketle, 24 saat maserasyon yöntemiyle hazırlanmış diklorometan (CRD) ve metanol (CRM) ekstrelerinin 48 saatlik maruziyette insan servikal kanser hücre hattındaki (HeLa) sitotoksik etkisinin araştırılması amaçlandı. Bitki ekstresinden hazırlanan farklı konsantrasyonların hücre canlılığına etkisi çalışma prensipleri farklı olan MTT ve xCELLigence GZHA sistemi kullanılarak araştırıldı ve IC50 değerleri belirlendi. Sonuç olarak, CRD ve CRM ekstrelerinin MTT bulguları, xCELLigence analiziyle tutarlı olup HeLa hücrelerinde sitotoksik etkiye sahip olduğu bulundu. C. lycaonica türünün kanser tedavisinde yeni bir doğal kaynak olarak değerlendirilebileceği düşünüldü. Bu çalışmanın, Türkiye’ye endemik birçok türün biyolojik aktivite ve etki mekanizmalarının aydınlatılmasını teşvik edeceği düşünülmektedir.

Keywords

Centaurea lycaonica Servikal kanser HeLa MTT xCELLigence

Supporting Institution

TÜBİTAK

Project Number

1919B012314005

Thanks

Hazırlanmış olan bu araştırma çalışması, TÜBİTAK 2209-A kapsamında 1919B012314005 kodu ile desteklenen bitirme projesinden üretilmiş olup katkılarından dolayı TÜBİTAK’a teşekkürlerimizi sunarız.

References

  • Artun, F. T., & Karagöz, A. (2021). Antiproliferative and apoptosis-inducing effects of the methanolic extract of Centaurea hermannii in human cervical cancer cell line. Biotechnic & Histochemistry, 96(1), 1-10. https://doi.org/10.1080/10520295.2020.1751288
  • Atmaca, H., Bozkurt, E., Kısım, A., & Uslu, R. (2016). Comparative analysis of XTT assay and xCELLigence system by measuring cytotoxicity of resveratrol in human cancer cell lines. Turkish Journal of Biochemistry, 41(6), 413-421. https://doi.org/10.1515/tjb-2016-0128
  • Ayad, R., & Akkal, S. (2019). Phytochemistry and biological activities of Algerian Centaurea and related genera. Studies in natural products chemistry, 63, 357-414. https://doi.org/10.1016/B978-0-12-817901-7.00012-5
  • Brown, A. J., & Trimble, C. L. (2012). New technologies for cervical cancer screening. Best practice & research Clinical obstetrics & gynaecology, 26(2), 233-242. https://doi.org/10.1016/j.bpobgyn.2011.11.001
  • Davis P.H. (1970). Flora of Turkey and the East Aegean Islands. Vol. 3; 548-625.
  • Delgado-Waldo, I., Contreras-Romero, C., Salazar-Aguilar, S., Pessoa, J., Mitre-Aguilar, I., García-Castillo, V., ... & Jacobo-Herrera, N. J. (2023). A triple-drug combination induces apoptosis in cervical cancer-derived cell lines. Frontiers in Oncology, 13, 1106667. https://doi.org/10.3389/fonc.2023.1106667
  • Erol-Dayı, Ö., Pekmez, M., Bona, M., Aras Perk, A., Arda, N. (2011). Total phenolic contents, antioxidant activities cytotoxicity of three Centaurea species: C. calcitrapa subsp. calcitrapa, C. ptosimopappa C. spicata. Free Radicals and Antioxidants, 1(2), 31-36. https://doi.org/10.5530/ax.2011.2.7
  • Fattaheian-Dehkordi, S., Hojjatifard, R., Saeedi, M., & Khanavi, M. (2021). A review on antidiabetic activity of Centaurea spp.: A new approach for developing herbal remedies. Evidence-based complementary and alternative medicine, 2021. https://doi.org/10.1155/2021/5587938
  • Gras, A., Parada, M., Pellicer, J., Vallès, J., & Garnatje, T. (2022). Cancer and Traditional Plant Knowledge, an Interesting Field to Explore: Data from the Catalan Linguistic Area. Molecules, 27(13), 4070. https://doi.org/10.3390/molecules27134070
  • Huang, W., Gfeller, V., & Erb, M. (2018). Root volatiles in plant-plant interactions II: Root terpenes from Centaurea stoebe modify Taraxacum officinale root chemistry and root herbivore growth. bioRxiv, 441790. https://doi.org/10.1101/441790
  • Jabri, T., Imran, M., Aziz, A., Rao, K., Kawish, M., Irfan, M., ... & Shah, M. R. (2019). Design and synthesis of mixed micellar system for enhanced anticancer efficacy of Paclitaxel through its co-delivery with Naringin. Drug development and industrial pharmacy, 45(5), 703-714. https://doi.org/10.1080/03639045.2018.1550091
  • Keser, S., Keser, F., Turkoglu, İ., Kaygılı, O., ... & Turkoglu S. (2020). In Vitro Biological Evaluation and Phytochemical Contents of Three Centaurea L. Species Growing from Eastern Anatolia in Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 23(1), 148-156. https://doi.org/10.18016/ksutarimdoga.vi.589279
  • Khammar, A., & Djeddi, S. (2012). Pharmacological and biological properties of some Centaurea species. Eur J Sci Res, 84(3), 398-416. http://www.europeanjournalofscientificresearch.com
  • Kısa, D., Çelik, A., & İmamoğlu, R. (2024). Assessment of Inhibitory Ability Against Medicinally Important Enzymes with Invitro and In Silico Studies: Phenolic Content of Endemic Centaurea cadmea subsp. pontica. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(1), 14-25. https://doi.org/10.18016/ksutarimdoga.vi.1294720
  • Koc, S., Isgor, B. S., Isgor, Y. G., Shomali Moghaddam, N., & Yildirim, O. (2015). The potential medicinal value of plants from Asteraceae family with antioxidant defense enzymes as biological targets. Pharmaceutical biology, 53(5), 746-751. https://doi.org/10.3109/13880209.2014.942788
  • Lopes, D. C. D. X. P., de Oliveira, T. B., Viçosa, A. L., Valverde, S. S., & Júnior, E. R. (2021). Anti-inflammatory activity of the compositae family and its therapeutic potential. Planta Medica, 87(01/02), 71-100. https://doi.org/10.1055/a-1178-5158
  • Lu, X., Yang, F., Chen, D., Zhao, Q., Chen, D., Ping, H., & Xing, N. (2020). Quercetin reverses docetaxel resistance in prostate cancer via androgen receptor and PI3K/Akt signaling pathways. International Journal of Biological Sciences, 16(7), 1121. https://doi.org/10.7150/ijbs.41686
  • Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods, 65(1-2), 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  • Oberg, H. H., Peters, C., Kabelitz, D., & Wesch, D. (2020). Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro. In Methods in Enzymology (Vol. 631, pp. 429-441). Academic Press. https://doi.org/10.1016/bs.mie.2019.07.020
  • Rajaram, S., & Gupta, B. (2021). Screening for cervical cancer: Choices & dilemmas. Indian Journal of Medical Research, 154(2), 210-220. https://doi.org/10.4103/ijmr.IJMR_857_20
  • Saklani, A., & Kutty, S. K. (2008). Plant-derived compounds in clinical trials. Drug discovery today, 13(3-4), 161-171. https://doi.org/10.1016/j.drudis.2007.10.010
  • Shaikh, R., Daniel, A., & Lyng, F. M. (2023). Raman Spectroscopy for Early Detection of Cervical Cancer, a Global Women’s Health Issue-A Review. Molecules, 28(6), 2502. https://doi.org/10.3390/molecules28062502
  • Sung, H., Ferlay, J., Siegel, R. L., Laversanne, M., Soerjomataram, I., Jemal, A., & Bray, F. (2021). Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians, 71(3), 209-249. https://doi.org/10.3322/caac.21660
  • Wu, L., Cao, K., Ni, Z., Wang, S., Li, W., Liu, X., & Chen, Z. (2019). Rhein reverses doxorubicin resistance in SMMC‐7721 liver cancer cells by inhibiting energy metabolism and inducing mitochondrial permeability transition pore opening. Biofactors, 45(1), 85-96. https://doi.org/10.1002/biof.1462
  • Xu, J., Liu, D., Niu, H., Zhu, G., Xu, Y., Ye, D., ... & Zhang, Q. (2017). Resveratrol reverses Doxorubicin resistance by inhibiting epithelial-mesenchymal transition (EMT) through modulating PTEN/Akt signaling pathway in gastric cancer. Journal of Experimental & Clinical Cancer Research, 36, 1-14. https://doi.org/10.1186/s13046-016-0487-8
  • Yuan, C. H., Horng, C. T., Lee, C. F., Chiang, N. N., Tsai, F. J., Lu, C. C., ... & Chen, F. A. (2017). Epigallocatechin gallate sensitizes cisplatin‐resistant oral cancer CAR cell apoptosis and autophagy through stimulating AKT/STAT3 pathway and suppressing multidrug resistance 1 signaling. Environmental toxicology, 32(3), 845-855. https://doi.org/10.1002/tox.22284
There are 26 citations in total.

Details

Primary Language Turkish
Subjects Structural Biology
Journal Section RESEARCH ARTICLE
Authors

Ayşe Kübra Karaboğa Arslan 0000-0002-4689-0657

Eylül Güngörenler 0009-0008-4009-268X

Leyla Paşayeva 0000-0003-3860-7222

Mehmet Bozkurt 0000-0003-2965-6719

Osman Tugay 0000-0003-3980-7648

Project Number 1919B012314005
Early Pub Date January 30, 2025
Publication Date February 12, 2025
Submission Date June 5, 2024
Acceptance Date December 31, 2024
Published in Issue Year 2025 Volume: 28 Issue: 1

Cite

APA Karaboğa Arslan, A. K., Güngörenler, E., Paşayeva, L., … Bozkurt, M. (2025). Centaurea lycaonica Boiss. & Heldr. Bitkisinin İnsan Servikal Kanser Hücre Hattında Sitotoksisitesinin MTT Testi ve xCELLigence Sistemi ile Değerlendirilmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(1), 47-52. https://doi.org/10.18016/ksutarimdoga.vi.1496499

Cited By

Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
https://doi.org/10.18016/ksutarimdoga.vi.1651205
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