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Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması

Year 2025, Volume: 28 Issue: 4, 945 - 954, 04.07.2028

Bülent Kaynak , Gülizar Aydoğdu , Zeynep Kolören , Onur Kolören

https://doi.org/10.18016/ksutarimdoga.vi.1651205

Abstract

Bu çalışmada, Ordu iline ait yöresel bitkilerinden Brassica oleracea var. viridis, Smilax excelsa ve Conium maculatum’un etanolik özütlerinin DNA hasarına karşı koruyucu potansiyelleri ve insan serviks adenokarsinomu (HeLa) hücre hattına karşı antikanser aktiviteleri araştırılmıştır. Özütler, farklı konsantrasyonlarda hazırlanarak antikanser aktiviteleri, HeLa hücre hattında 3-(4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolyum bromid (MTT) testi ile değerlendirilmiştir. Özütlerin, hidroksil radikali (-OH) ile indüklenen pBR322 plazmid DNA hasarını engelleme potansiyelleri de araştırılmıştır. Özütlerin HeLa hücreleri ile yapılan MTT analizleri neticesinde, farklı konsantrasyonlarla yapılan uygulamalarda, hücre canlılığının doza bağlı olarak azaldığı gözlenmiştir. S. excelsa, B. oleracea var. viridis ve C. maculatum özütlerinin 24 saatteki yaklaşık IC50 değerleri sırasıyla 3.88, 2.20 ve 1.48 mg/mL, 48 saatteki yaklaşık IC50 değerleri ise sırasıyla 1.82, 1.13 ve 0.39 mg/mL olarak tespit edilmiştir. Ayrıca, -OH ile indüklenen DNA hasarını önleme açısından özütlerin farklı (20, 10 ve 5 mg/mL) konsantrasyonlarının güçlü koruyucu aktivite gösterdiği bulunmuştur.
Bu bulgular, S. excelsa, B. oleracea var. viridis ve C. maculatum etanolik özütlerinin DNA koruyucu ve antikanser etkilerine dair öncü veriler sunmakta olup, ileri çalışmalarda bu etkilerden sorumlu aktif bileşiklerin belirlenmesi ve terapötik potansiyellerinin detaylı olarak incelenmesi gerektiğini ortaya koymaktadır.

Keywords

[Brassica oleracea var. viridis Conium maculatum HeLa pBR322 Plazmid DNA Smilax excelsa

Ethical Statement

Etik onay gerekmemektedir.

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Investigation of the Anticancer Effects on Human Cervical Cancer (HeLa) Cell Line and the DNA Protective Activities of Traditional Plants from Ordu Province (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum)

Year 2025, Volume: 28 Issue: 4, 945 - 954, 04.07.2028

Bülent Kaynak , Gülizar Aydoğdu , Zeynep Kolören , Onur Kolören

https://doi.org/10.18016/ksutarimdoga.vi.1651205

Abstract

This study investigates the protective potential of ethanolic extracts of local plants from Ordu province, Brassica oleracea var. viridis, Smilax excelsa, and Conium maculatum, against DNA damage and their anticancer activities in HeLa (cervix adenocarcinoma) cells. The extracts were prepared at varying concentrations, and their anticancer effects were evaluated using the 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) assay in HeLa cells. The ability of these extracts to prevent hydroxyl radical (-OH)-induced damage to pBR322 plasmid DNA was also examined. MTT assay results showed that cell viability decreased in a dose-dependent manner when treated with different concentrations of the extracts. The approximate IC50 values for S. excelsa, B. oleracea var. viridis, and C. maculatum extracts at 24 hours were determined to be 3.88, 2.20, and 1.48 mg/mL, respectively, while at 48 hours, these values were 1.82, 1.13, and 0.39 mg/mL. Furthermore, the extracts demonstrated strong protective activity at various concentrations (20, 10, and 5 mg/mL) in preventing OH-induced DNA damage. These findings provide preliminary data on the DNA protective and anticancer effects of ethanol extracts of S. excelsa, B. oleracea var. viridis, and C. maculatum, indicating that future studies should focus on identifying the active compounds responsible for these effects and investigating their therapeutic potential in more detail.

Keywords

[Brassica oleracea var. viridis Conium maculatum HeLa pBR322 Plasmid DNA Smilax excelsa

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There are 45 citations in total.

Details

Primary Language Turkish
Subjects Plant Cell and Molecular Biology
Journal Section RESEARCH ARTICLE
Authors

Bülent Kaynak 0000-0002-9207-5485

Gülizar Aydoğdu 0000-0001-8390-1477

Zeynep Kolören 0000-0001-9708-2716

Onur Kolören 0000-0002-3359-4904

Early Pub Date June 10, 2025
Publication Date July 4, 2028
Submission Date March 4, 2025
Acceptance Date May 14, 2025
Published in Issue Year 2025 Volume: 28 Issue: 4

Cite

APA Kaynak, B., Aydoğdu, G., Kolören, Z., Kolören, O. (2028). Ordu İli Yöresel Bitkilerinin (Brassica oleracea var. viridis, Smilax excelsa, Conium maculatum) İnsan Serviks Kanseri (HeLa) Hücre Hattına Karşı Antikanser Etkilerinin ve DNA Koruyucu Aktivitelerinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 945-954. https://doi.org/10.18016/ksutarimdoga.vi.1651205
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