Partial Purification and Characterization of Polyphenol Oxidase Enzyme from Common-Morning Glory (Ipomoea purpurea)

Bektore Mansurov; Elif Kale; Arda Acemi; Yonca Yüzügüllü Karakuş

doi:10.18016/ksutarimdoga.vi.1008243

EN TR

Partial Purification and Characterization of Polyphenol Oxidase Enzyme from Common-Morning Glory (Ipomoea purpurea)

Abstract

This study aimed to purify and biochemically characterize polyphenol oxidase (PPO) enzyme from the plant Ipomoea purpurea (I. purpurea) for the first time. For this purpose, the crude extract sample obtained from the extraction of in vitro cultured plant leaves under optimum conditions (25 mgmL-1 Polyvinylpolypyrrolidone, pH 7.0) was subjected to three-phase partitioning, and the PPO enzyme was 10.5-fold purified with a 57% activity recovery. The optimum pH and temperature values were determined as 7.0 and 30°C, respectively. Laccase, peroxidase, and catechol oxidase activities were observed after activity staining of partially purified enzyme. From stability tests, it was noted that more than 75% and 65% of its original activity were maintained at temperatures 20℃-40℃ and pH 7.0-9.0, respectively.

Keywords

Gündüz Sefası (Ipomoea purpurea) Bitkisinden Polifenol Oksidaz Enziminin Kısmi Saflaştırılması ve Karakterizasyonu

Öz

Bu çalışmada, polifenol oksidaz (PPO) enziminin Ipomoea purpurea (I. purpurea) bitkisinden ilk kez saflaştırılması ve enzimin biyokimyasal özelliklerinin belirlenmesi amaçlandı. Buna göre, in vitro olarak üretilen bitkiye ait yapraklardan optimum koşullarda (25 mg ml-1 Polivinilpolipirrolidon, pH 7.0) gerçekleştirilen ekstraksiyon sonrası elde edilen ham ekstrakt örneğinden PPO enzimi üçlü faz ayırma tekniği ile yaklaşık %57 aktivite geri kazanımla 10,5 kat saflaştırıldı. Enzimin optimum pH ve sıcaklık değerleri sırasıyla 7.0 ve 30°C olarak belirlendi. Aktivite boyama sonrası kısmen saf enzimde lakkaz, peroksidaz ve katekol oksidaz aktiviteleri tespit edildi. Ayrıca, 20°C ile 40°C arasında enzim aktivitesinin ≥%75, pH 7.0 ile 9.0 arasında ≥%65 oranında korunduğu tespit edildi.

Anahtar Kelimeler

Supporting Institution

Kocaeli Üniversitesi

Project Number

FMP-2021-2448

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Bektore Mansurov
0000-0002-4262-9419
Türkiye

Elif Kale
0000-0002-4266-9517
Türkiye

Arda Acemi
0000-0003-0270-8507
Türkiye

Yonca Yüzügüllü Karakuş ^*
0000-0003-0286-8711
Türkiye

Publication Date

December 30, 2022

Submission Date

October 11, 2021

Acceptance Date

February 17, 2022

Published in Issue

Year 2022 Volume: 25 Number: Ek Sayı 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1008243

IZ

https://izlik.org/JA72DU74BF

Cite

RIS / Bibtex

APA

Mansurov, B., Kale, E., Acemi, A., & Yüzügüllü Karakuş, Y. (2022). Partial Purification and Characterization of Polyphenol Oxidase Enzyme from Common-Morning Glory (Ipomoea purpurea). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 1), 22-32. https://doi.org/10.18016/ksutarimdoga.vi.1008243

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