Türkiye’de Yetişen Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez Bitkisinin Serbest Radikal Süpürücü, Anti-inflamatuvar ve Hipoglisemik Aktivitelerinin Değerlendirilmesi

Asli Can Agca; Sezen Yılmaz Sarıaltın; Betül Sever Yılmaz

doi:10.18016/ksutarimdoga.vi.1015574

TR EN

Türkiye’de Yetişen Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez Bitkisinin Serbest Radikal Süpürücü, Anti-inflamatuvar ve Hipoglisemik Aktivitelerinin Değerlendirilmesi

Öz

Helianthemum Türkiye’de 4’ü endemik olmak üzere 19 takson ile temsil edilmektedir. Bu çalışmada Türkiye’de doğal olarak yetişen Helianthemum oelandicum subsp. incanum toprak üstü kısımlarının etanollü (%75) ve sulu ekstrelerinin antioksidan, anti-inflamatuvar ve hipoglisemik aktiviteleri araştırılmıştır. Sulu ekstrenin DPPH ve ABTS radikalleri üzerine gösterdiği süpürücü etki miktarı sırasıyla 67.26±0.49 ve 68.08±0.41 µg ml-1 olarak tespit edilmiştir. Bitkinin etanollü ekstresinin DPPH radikaline karşı gösterdiği antioksidan aktivite değeri 46.71±0.27 µg ml-1 iken, ABTS radikali için bu değer 51.60±0.76 µg ml-1 olarak bulunmuştur. Bitkinin hem etanollü hem de sulu ekstresinin eritrosit membran hemolizini konsantrasyona bağlı olarak inhibe ederek in vitro anti-inflamatuvar etki gösterdiği ve etanollü ekstrenin sulu ekstreye göre daha güçlü etkiye sahip olduğu belirlenmiştir. Bitki ekstrelerinin in vitro hipoglisemik aktivite tayini için α-glukozidaza karşı inhibisyon etkileri ölçülmüş, etanollü ve sulu ekstrelerin IC50 değerleri sırasıyla 2.52±0.01 ve 3.21±0.01 µg ml-1 bulunmuş ve standart bileşik akarboz ile karşılaştırıldığında (IC50= 0.90±0.01 µg ml-1) α-glukozidaza karşı güçlü inhibisyon gösterdikleri tespit edilmiştir. Tüm aktivite sonuçları bir arada değerlendirildiğinde etanollü ekstrenin antioksidan, anti-inflamatuvar ve hipoglisemik aktivitesinin sulu ekstreden daha yüksek olduğu bulunmuştur.

Anahtar Kelimeler

Evaluation of Free Radical Scavenging, Anti-inflammatory and Hypoglycemic Activity of Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez from Türkiye

Abstract

Helianthemum is represented by 19 taxa, and out of four are endemic in Türkiye. The antioxidant, anti-inflammatory, and hypoglycemic activities of the ethanol (75%) and aqueous extracts from aerial parts of Helianthemum oelandicum subsp. incanum growing in Türkiye was examined in this study. The water extract exhibited antioxidant activity with 67.26±0.49 and 68.08±0.41 µg ml-1 values on DPPH and ABTS radicals, respectively. The antioxidant activity value of ethanol extract on DPPH radical was found as 46.71±0.27 µg ml-1, where as this value was determined as 51.60±0.76 µg ml-1 for scavenging effects on ABTS radical. Both ethanol and aqueous extracts possessed in vitro anti-inflammatory activity by inhibiting erythrocyte membrane hemolysis in a concentration dependent manner and ethanol extracts exhibited greater activity than aqueous extracts. By measuring the inhibitory effect of the extracts on α-glucosidase, in vitro hypoglycemic activity was assessed. According to the results of this study, the IC50 values of both ethanolic and aqueous extracts were found to be close to each other, 2.52±0.01 and 3.21±0.01 µg ml-1, respectively and compared with the standard compound acarbose (IC50= 0.90±0.01 µg ml-1) it was determined that both extracts exhibited strong inhibition on α-glucosidase. Based on all results evaluated, the ethanolic extract displayed higher antioxidant, anti-inflammatory, and hypoglycemic activities than the aqueous extract.

Keywords

Thanks

The authors would like to thank to Prof. Dr. Hayri Duman for identification of the plant.

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Asli Can Agca ^*
0000-0002-5710-3479
Türkiye

Sezen Yılmaz Sarıaltın
0000-0002-8387-4146
Türkiye

Betül Sever Yılmaz
Türkiye

Publication Date

April 30, 2023

Submission Date

October 27, 2021

Acceptance Date

March 3, 2022

Published in Issue

Year 2023 Volume: 26 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1015574

IZ

https://izlik.org/JA79FY35LL

Cite

RIS / Bibtex

APA

Can Agca, A., Yılmaz Sarıaltın, S., & Sever Yılmaz, B. (2023). Türkiye’de Yetişen Helianthemum oelandicum subsp. incanum (Willk.) G. Lopez Bitkisinin Serbest Radikal Süpürücü, Anti-inflamatuvar ve Hipoglisemik Aktivitelerinin Değerlendirilmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(2), 227-233. https://doi.org/10.18016/ksutarimdoga.vi.1015574

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