Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey

Selin Ceren Balsak; Seher Gül Avcu; Nihal Buzkan

doi:10.18016/ksutarimdoga.vi.1048580

TR EN

Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey

Abstract

In this study, Tobacco mild green mosaic virus (TMGMV) infection was investigated in pepper crops from two regions having high economical importance in Turkey. A total of 397 leaf and fruit samples showing mosaic, yellowing, mottling, and pitting symptoms were collected to test by double antibody sandwich ELISA (DAS-ELISA) using polyclonal antiserum. DAS-ELISA results indicated that 97 out of all tested samples (24.4%) were found to be infected with TMGMV. Samples resulting positive for TMGMV infection were used to amplify the virus coat protein (CP) gene sequences with specific primers by RT-PCR for further molecular characterization of the virus isolates. A comparison of the CP sequences of the virus isolates revealed that the identity of nucleotides ranged between 97.2 and 100%, and the amino acid similarity ranged between 96.8 and 100% among themselves. To the best of our knowledge, this is the first report of TMGMV infection in Capsicum annuum in Turkey.

Keywords

Supporting Institution

This research was funded by the the Research Fund of Kahramanmaraş Sütçü Imam University

Project Number

2021/1-15YLS

References

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Selin Ceren Balsak ^*
0000-0002-2326-7520
Türkiye

Seher Gül Avcu
0000-0002-5499-6611
Türkiye

Nihal Buzkan
0000-0002-0428-0447
Türkiye

Publication Date

December 30, 2022

Submission Date

December 27, 2021

Acceptance Date

June 12, 2022

Published in Issue

Year 2022 Volume: 25 Number: Ek Sayı 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1048580

IZ

https://izlik.org/JA66NY34SJ

Cite

RIS / Bibtex

APA

Balsak, S. C., Avcu, S. G., & Buzkan, N. (2022). Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 2), 490-496. https://doi.org/10.18016/ksutarimdoga.vi.1048580

AMA

1.Balsak SC, Avcu SG, Buzkan N. Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey. KSU J. Agric Nat. 2022;25(Ek Sayı 2):490-496. doi:10.18016/ksutarimdoga.vi.1048580

Chicago

Balsak, Selin Ceren, Seher Gül Avcu, and Nihal Buzkan. 2022. “Detection and Molecular Characterization of Tobacco Mild Green Mosaic Virus Isolates Infecting Peppers in Turkey”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 25 (Ek Sayı 2): 490-96. https://doi.org/10.18016/ksutarimdoga.vi.1048580.

EndNote

Balsak SC, Avcu SG, Buzkan N (December 1, 2022) Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 25 Ek Sayı 2 490–496.

IEEE

[1]S. C. Balsak, S. G. Avcu, and N. Buzkan, “Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey”, KSU J. Agric Nat., vol. 25, no. Ek Sayı 2, pp. 490–496, Dec. 2022, doi: 10.18016/ksutarimdoga.vi.1048580.

ISNAD

Balsak, Selin Ceren - Avcu, Seher Gül - Buzkan, Nihal. “Detection and Molecular Characterization of Tobacco Mild Green Mosaic Virus Isolates Infecting Peppers in Turkey”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 25/Ek Sayı 2 (December 1, 2022): 490-496. https://doi.org/10.18016/ksutarimdoga.vi.1048580.

JAMA

1.Balsak SC, Avcu SG, Buzkan N. Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey. KSU J. Agric Nat. 2022;25:490–496.

MLA

Balsak, Selin Ceren, et al. “Detection and Molecular Characterization of Tobacco Mild Green Mosaic Virus Isolates Infecting Peppers in Turkey”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 25, no. Ek Sayı 2, Dec. 2022, pp. 490-6, doi:10.18016/ksutarimdoga.vi.1048580.

Vancouver

1.Selin Ceren Balsak, Seher Gül Avcu, Nihal Buzkan. Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey. KSU J. Agric Nat. 2022 Dec. 1;25(Ek Sayı 2):490-6. doi:10.18016/ksutarimdoga.vi.1048580

Cited By

Genetic Diversity of Tobacco mosaic virus (TMV) isolates from tobacco growing fields of Western Anatolia, Türkiye

Tekirdağ Ziraat Fakültesi Dergisi

https://doi.org/10.33462/jotaf.1413398

Türkiye'de Biberde Enfeksiyon Oluşturan Tobacco mild green mosaic virus İzolatlarının Belirlenmesi ve Moleküler Karakterizasyonu

Abstract

Keywords

Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey

Abstract

Keywords

Supporting Institution

Project Number

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Genetic Diversity of Tobacco mosaic virus (TMV) isolates from tobacco growing fields of Western Anatolia, Türkiye