Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey

Selin Ceren Balsak; Seher Gül Avcu; Nihal Buzkan

doi:10.18016/ksutarimdoga.vi.1048580

TR EN

Türkiye'de Biberde Enfeksiyon Oluşturan Tobacco mild green mosaic virus İzolatlarının Belirlenmesi ve Moleküler Karakterizasyonu

Öz

Bu çalışmada, Türkiye’de biber yetiştiriciliğinin ekonomik olarak önemli olduğu iki bölgede Tobacco mild green mosaic virus (TMGMV) enfeksiyonu incelenmiştir. Mozaik, sararma, beneklenme ve çukurlaşma simptomu gösteren toplam 397 yaprak ve meyve örneği toplanmış ve double antibody sandwich ELISA (DAS-ELISA) ile poliklonal antiserum kullanılarak testlenmiştir. DAS-ELISA testinde testlenen örnekler içerisinde 97 örneğin (%24.4) TMGMV enfeksiyonuna sahip olduğu belirlenmiştir. TMGMV ile enfekteli pozitif örnekler, gen spesifik primerler kullanılarak RT-PCR yöntemiyle virüs kılıf protein (KP) geninin çoğaltılması ve izolatların moleküler karakterizasyonu için kullanılmıştır. KP nükleotid dizilerinin karşılaştırması sonucunda, izolatların kendi aralarında nükleotid benzerliğinin %97.2-100, amino asit benzerliğinin ise %96.8-100 arasında değiştiği belirlenmiştir. Bildiğimiz kadarıyla, bu Türkiye'de TMGMV’nin enfeksiyonunun Capsicum annuum'daki ilk raporudur.

Anahtar Kelimeler

Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey

Abstract

In this study, Tobacco mild green mosaic virus (TMGMV) infection was investigated in pepper crops from two regions having high economical importance in Turkey. A total of 397 leaf and fruit samples showing mosaic, yellowing, mottling, and pitting symptoms were collected to test by double antibody sandwich ELISA (DAS-ELISA) using polyclonal antiserum. DAS-ELISA results indicated that 97 out of all tested samples (24.4%) were found to be infected with TMGMV. Samples resulting positive for TMGMV infection were used to amplify the virus coat protein (CP) gene sequences with specific primers by RT-PCR for further molecular characterization of the virus isolates. A comparison of the CP sequences of the virus isolates revealed that the identity of nucleotides ranged between 97.2 and 100%, and the amino acid similarity ranged between 96.8 and 100% among themselves. To the best of our knowledge, this is the first report of TMGMV infection in Capsicum annuum in Turkey.

Keywords

Supporting Institution

This research was funded by the the Research Fund of Kahramanmaraş Sütçü Imam University

Project Number

2021/1-15YLS

References

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Selin Ceren Balsak ^*
0000-0002-2326-7520
Türkiye

Seher Gül Avcu
0000-0002-5499-6611
Türkiye

Nihal Buzkan
0000-0002-0428-0447
Türkiye

Publication Date

December 30, 2022

Submission Date

December 27, 2021

Acceptance Date

June 12, 2022

Published in Issue

Year 2022 Volume: 25 Number: Ek Sayı 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1048580

IZ

https://izlik.org/JA66NY34SJ

Cite

RIS / Bibtex

APA

Balsak, S. C., Avcu, S. G., & Buzkan, N. (2022). Detection and Molecular Characterization of Tobacco mild green mosaic virus Isolates Infecting Peppers in Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 2), 490-496. https://doi.org/10.18016/ksutarimdoga.vi.1048580

Cited By

Genetic Diversity of Tobacco mosaic virus (TMV) isolates from tobacco growing fields of Western Anatolia, Türkiye

Tekirdağ Ziraat Fakültesi Dergisi

https://doi.org/10.33462/jotaf.1413398