Saccharomyces cerevisiae L. Hücre Kültürü Oksidatif Stres Modelinde Bazı Fosfazen Türevlerinin Biyokimyasal Aktiviteleri

Ayşe Dilek Özşahin; Ayşe Erdoğdu; Oğuz Ayhan Kireçci; Fatih Aslan; Ali İhsan; Prof. Dr. Ökkeş Yılmaz

doi:10.18016/ksutarimdoga.vi.1051663

EN TR

Antioxidant Effects of Some Phosphazene Derivatives in Saccharomyces cerevisiae L. Cell Culture Oxidative Stress Model

Abstract

In this study, biochemical activities of some phosphazenes were determined in Saccharomyces cerevisiae L. culture medium. Different phosphazene molecules were used in experimental practice. The experimental groups within the scope of the study were organized as control group, H2O2 (Hydrogen peroxide) and groups of phosphazen molecules. After the groups were prepared, 30 µg of phosphazen and 100 µl of H2O2 were added to other cultures, except for the control group of S. cerevisiae culture. It was incubated for 72 hours at 30 °C. At the end of incubation, cell pellets were separated. Glutathione S-Transferase (GST) and Total protein levels were determined from supernatant. The fatty acid and lipophilic molecules were analyzed from the homogenate obtained with the hexane / isopropanol alcohol mixture. According to our experimental results, while total protein values and GST values increased in parallel with the phosphazen molecule and H2O2 added yeast cells, GST levels were decreased in some groups, although an increase in the amount of protein was observed. Ergosterol, which has an important place in the membrane structure of S. cerevisiae, was found to be higher in T3 and T3B coded phosphazenes and H2O2 groups compared to the control group, and low in T4 coded phosphazenes and H2O2 groups. Our study results revealed that as a result of the addition of phosphazen and H2O2 molecules to the culture medium of S. cerevisiae, ergosterol and fatty acid synthesis, fatty acids cause increases or decreases in the end products of enzymes that double-enter the hydrocarbon chain.

Keywords

Saccharomyces cerevisiae L. Hücre Kültürü Oksidatif Stres Modelinde Bazı Fosfazen Türevlerinin Biyokimyasal Aktiviteleri

Öz

Bu çalışmada, Saccharomyces cerevisiae L. kültür ortamında bazı fosfazenlerin biyokimyasal aktiviteleri belirlendi. Deneysel uygulamada farklı fosfazen molekülleri kullanıldı. Çalışma kapsamındaki deney grupları; kontrol grubu, H2O2 (Hidrojen peroksit) ve fosfazen molekülleri grupları şeklinde düzenlendi. Gruplar hazırlandıktan sonra, kontrol grubu dışında diğer kültürlere 30 µg fosfazen ve 100 µl H2O2 ilave edildi. 30 °C de 72 saat inkübasyona bırakıldı. İnkübasyon sonunda hücre peletleri ayrıldı. Elde edilen süpernatantdan Glutatyon S-Transferaz (GST) ile total protein düzeyleri belirlendi. Hekzan/izopropanol alkol karışımı ile elde edilen homojenattan da yağ asidi ve lipofilik moleküllerin analizi yapıldı. Deney sonuçlarına göre, fosfazen molekülü ve H2O2 ilave edilen maya hücrelerinde total protein değerleri ile GST değerlerinde paralel bir artış gözlenirken, bazı gruplarda protein miktarında artış saptandığı halde GST düzeyinde azalma olduğu belirlendi. S. cerevisiae’ nin membran yapısında önemli bir yer kaplayan ergosterolün, T3 ve T3B kodlu fosfazenler ile H2O2 gruplarında kontrol grubuna göre yüksek, T4 kodlu fosfazenler ile H2O2 gruplarında ise düşük olduğu belirlendi. Sonuç olarak; S. cerevisiae kültür ortamına fosfazen ve H2O2 moleküllerin eklenmesinin, ergosterol ve yağ asidi sentezi ile yağ asitlerinin hidrokarbon zincirine çift bağ girişi yapan enzimlerin son ürünlerinde artışlara ya da azalışlara neden olduğu ortaya konulmuştur.

Anahtar Kelimeler

Supporting Institution

Bitlis Eren Üniversitesi Rektörlüğü Bilimsel Araştırma Projeleri (BAP) birimi

Project Number

BEBAP 2019.008

Thanks

Bu çalışma. Bitlis Eren Üniversitesi Rektörlüğü Bilimsel Araştırma Projeleri (BAP) birimi tarafından BEBAP 2019.008 nolu proje numarası ile desteklenmiştir.

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Details

Primary Language

Turkish

Subjects

Structural Biology

Journal Section

Research Article

Authors

Ayşe Dilek Özşahin ^*
0000-0002-1832-7082
Türkiye

Ayşe Erdoğdu
0000-0001-5894-1442
Türkiye

Oğuz Ayhan Kireçci
0000-0003-2205-4758
Türkiye

Fatih Aslan
0000-0002-5948-6979
Türkiye

Ali İhsan
0000-0002-3912-0670
Türkiye

Prof. Dr. Ökkeş Yılmaz
0000-0002-8276-4498
Türkiye

Publication Date

February 28, 2023

Submission Date

December 31, 2021

Acceptance Date

March 10, 2022

Published in Issue

Year 2023 Volume: 26 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1051663

IZ

https://izlik.org/JA29US69WK

Cite

RIS / Bibtex

APA

Özşahin, A. D., Erdoğdu, A., Kireçci, O. A., Aslan, F., İhsan, A., & Yılmaz, P. D. Ö. (2023). Saccharomyces cerevisiae L. Hücre Kültürü Oksidatif Stres Modelinde Bazı Fosfazen Türevlerinin Biyokimyasal Aktiviteleri. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(1), 1-10. https://doi.org/10.18016/ksutarimdoga.vi.1051663