Effects of Pistacia terebinthus L. Subsp. palaestina and Rhus coriaria L. Plants on Some Biochemical Parameters of Brain Tissue of Sprague-Dawley Rats in Experimental Breast Cancer Model

Figen Erdem Erişir; Oğuz Ayhan Kireçci; Ayşe Dilek Özşahin; Prof. Dr. Ökkeş Yılmaz

doi:10.18016/ksutarimdoga.vi.1074615

EN TR

Effects of Pistacia terebinthus L. Subsp. palaestina and Rhus coriaria L. Plants on Some Biochemical Parameters of Brain Tissue of Sprague-Dawley Rats in Experimental Breast Cancer Model

Abstract

In this study, the therapeutic effects of Pistacia terebinthus L. subsp. Palaestina (terebinth) and Rhus coriaria L (sumac) plants on DMBA-induced breast cancer in 66 female Sprague-Dawley rats were investigated through biochemical analysis. The rats were divided into 6 groups as Control, DMBA (7.12-Dimethylbenzanthracene), PT (terebinth), RC (sumac), PT+DMBA and RC+DMBA. DMBA was administered to 8-week-old rats via gavage, a single dose of 80 mg/kg according to body weight. Aqueous extracts of terebinth and sumac were given orally to rats in antioxidant groups 3 days a week. In biochemical studies, changes in activities of the antioxidant enzymes CAT (catalase), GST (glutathione transferase), and SOD (superoxide dismutase) in brain tissues as well as total protein, MDA (malondialdehyde), GSH (glutathione), fatty acid, and vitamin levels were determined. Total protein levels generally reduced in the DMBA group compared to the control group (p<0.05)while the levels of the MDA in the DMBA brain tissue groups elevated compared to the control group and reduced in antioxidant groups (p<0.01; p<0.001). Cholesterol levels and lipophilic vitamins were determined by HPLC. Their grades were different in the DMBA and antioxidant groups. Fatty acids were analyzed by GC. As a result of analysis, fatty acids such as palmitic, palmitoleic, stearic, oleic, linoleic, arachidonic, and docosahexaenoic were high in the tissues examined (p<0.01; p<0.001). The fatty acid levels were also found to vary in the DMBA and antioxidant tissue groups. These data suggest that Pistacia terebinthus and Rhus coriaria plants can be used against breast cancer and to reduce its negative outcomes, and studies should be continued for their safe medical application.

Keywords

Sprague-Dawley Sıçanlarda Deneysel Meme Kanseri Modelinde Pistacia terebinthus L. Subsp palaestina ve Rhus coriaria L. Bitkilerinin Beyin Dokusunun Bazı Biyokimyasal Parametreleri Üzerindeki Etkileri

Öz

Bu çalışmada, dişi 66 Sprague-Dawley ratında DMBA ile indüklenen meme kanserine karşı Pistacia terebinthus L. subsp Palaestina (terebinth) ve Rhus coriaria L. (sumak)’nin iyileştirici etkileri biyokimyasal olarak incelendi. Sıçanlar, Kontrol, DMBA (7.12-Dimetilbenzantrasene), PT (menengiç), RC (sumak), PT+DMBA ve RC+DMBA olmak üzere 6 gruba ayrıldı. DMBA, vücut ağırlıklarına göre, tek doz 80 mg/kg olacak şekilde, gavaj yoluyla, 8 haftalık dişi Sprague-Dawley sıçanlara uygulandı. Antioksidan gruplarındaki ratlara oral yolla menengiç ve sumak’ın sulu ekstraktları verildi. Biyokimyasal çalışmalarda beyin dokusunda antioksidan enzimler olan CAT (katalaz), GST (glutatyon transferaz) ve SOD (süperoksit dismutaz) aktivitelerindeki değişiklikler, total protein, MDA, (malondialdehit) GSH (glutatyon), yağ asidi ve vitamin düzeyleri belirlendi. Toplam protein seviyelerinin genellikle DMBA grubunda kontrole göre azaldığı gözlendi (p<0.05). DMBA beyin dokusu gruplarındaki MDA seviyeleri kontrol grubuna göre arttığı; antioksidan gruplarında azaldığı saptandı (p<0.01; p<0.001). Kolesterol seviyeleri ve lipofilik vitaminler HPLC ile belirlendi. DMBA ve antioksidan gruplarında bunların derecelerinin farklı olduğu görüldü. Yağ asitleri GC ile analiz edildi. Analiz sonucunda dokularda palmitik, palmitoleik, stearik, oleik, linoleik, araşidonik, dokosaheksaenoik gibi yağ asitlerinin yüksek olduğu bulundu (p<0.01; p<0.001). Bu yağ asidi seviyeleri DMBA ve antioksidan doku gruplarında değişiklikler gösterdi. Bu veriler, Pistacia terebinthus ve Rhus coriaria bitkilerinin meme kanseri ve olumsuzluklarına karşı bir uygulama olarak kullanılabileceği, güvenli tıbbi uygulaması için çalışmaların sürdürülmesi önerilir.

Anahtar Kelimeler

Supporting Institution

Fırat Üniversitesi

Project Number

FUBAP FF. 18.14

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Figen Erdem Erişir
0000-0002-0499-9339
Türkiye

Oğuz Ayhan Kireçci
0000-0003-2205-4758
Türkiye

Ayşe Dilek Özşahin ^*
0000-0002-1832-7082
Türkiye

Prof. Dr. Ökkeş Yılmaz
0000-0002-8276-4498
Türkiye

Publication Date

December 30, 2022

Submission Date

February 16, 2022

Acceptance Date

July 18, 2022

Published in Issue

Year 2022 Volume: 25 Number: Ek Sayı 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1074615

IZ

https://izlik.org/JA73YX55NF

Cite

RIS / Bibtex

APA

Erdem Erişir, F., Kireçci, O. A., Özşahin, A. D., & Yılmaz, P. D. Ö. (2022). Effects of Pistacia terebinthus L. Subsp. palaestina and Rhus coriaria L. Plants on Some Biochemical Parameters of Brain Tissue of Sprague-Dawley Rats in Experimental Breast Cancer Model. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 1), 33-41. https://doi.org/10.18016/ksutarimdoga.vi.1074615

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