Antibacterial Effects of Phlomoides molucelloides (Bunge) Salmaki

Anfal Izaldeen Al Kateeb; Enis Fuat Tüfekci; Mehmet Cengiz Baloğlu; Gokhan Zengin; Evren Yıldıztugay; Yasemin Çelik Altunoğlu

doi:10.18016/ksutarimdoga.v25i73358.1077985

TR EN

Phlomoides molucelloides (Bunge) Salmaki'nin Antibakteriyel Etkileri

Öz

Antibiyotiklere dirençli bakterilerle mücadelede mevcut antibiyotiklerin yetersizliği, yeni ve etkili antibakteriyel ilaçların keşfini zorunlu kılmıştır. Bakterilerin quorum sensing mekanizması ile çeşitli virülans faktörlerini sentezlediğinin keşfedilmesi, quorum sensing inhibitörlerinin enfeksiyon hastalıkları ile mücadelede kullanılabileceğini düşündürmüştür. Bu çalışmada, Phlomoides molucelloides (Bunge) Salmaki türünün toprak üstü ve kök kısımlarının metanol ve su özütlerinin antibakteriyel ve anti-quorum sensing aktivitelerinin araştırılması amaçlanmıştır. Özütlerin referans bakteri suşlarına karşı minimal inhibitör konsantrasyon (MİK) değerleri sıvı mikrodilüsyon yöntemi ile araştırılmıştır. Anti-quorum sensing aktivite, Chromobacterium violaceum ATCC 12472 ve Pseudomonas aeruginosa PAO1 biyoraportör suşlarda viyolasin ve piyosiyanin pigment üretiminin ve swarming (yayılma) hareketinin inhibisyonu üzerine belirlenmiştir. Antibiyofilm aktivite testi P. aeruginosa PAO1 suşuna karşı kristal viyole yöntemi ile değerlendirilmiştir. Tüm özütlerin test edilen bakterilere karşı çalışılan konsantrasyonlarda MİK değeri (> 2000 µg/mL) tespit edilmemiştir. Buna karşılık tüm özütlerin P. aeruginosa’nın swarming hareketini baskıladığı saptanmıştır. Ayrıca, bitkinin toprak üstü kısımlarının metanol özütlerinin bakteri üremesini baskılamadan piyosiyanin üretimini kontrole göre %81.7 oranında inhibe ettiği belirlenmiştir. Tüm özütlerin biyofilm oluşumu ve viyolasin pigment üretimi üzerine önemli bir inhibitör etkinliği saptanmamıştır. Sonuçlar P. molucelloides özütlerinin iyi bir anti-quorum sensing ajanı olabileceğini göstermiştir. Bu biyolojik aktivitelerin altında yatan mekanizmaları aydınlatmak amacıyla ileri çalışmaların yapılması gerekmektedir.

Anahtar Kelimeler

Antibacterial Effects of Phlomoides molucelloides (Bunge) Salmaki

Abstract

The insufficiency of existing antibiotics in the combat against antibiotics-resistant bacteria has necessitated the discovery of new and effective antibacterial drugs. The discovery that bacteria synthesize various virulence factors by the quorum sensing system has suggested that quorum sensing inhibitors may be used in the fight against infectious diseases. This study aimed to determine the antibacterial and anti-quorum sensing activities of methanol and water extracts of aerial and root parts of Phlomoides molucelloides (Bunge) Salmaki. The minimal inhibitory concentration (MIC) values of the extracts were investigated against reference bacterial strains using the broth microdilution method. Anti-quorum sensing activities were examined by violacein and pyocyanin pigments inhibition and swarming motility inhibition assays against Chromobacterium violaceum ATCC 12472 and Pseudomonas aeruginosa PAO1 bioreporter strains. The antibiofilm activities of the extracts were tested against P. aeruginosa PAO1 using the crystal violet staining method. The MIC value (> 2000 µg/mL) of all extracts against the tested bacteria could not be determined at the concentrations studied. All extracts partially inhibited the swarming motility of P. aeruginosa. Methanol extract of the aerial part inhibited pyocyanin production by 81.7% without interfering with P. aeruginosa growth. The extracts had no significant inhibitory activity on biofilm formation and violacein pigment production. These results showed that the extracts of P. molucelloides may be good anti-quorum sensing agents. Further research can be done to elucidate the mechanisms underlying these biological activities.

Keywords

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Anfal Izaldeen Al Kateeb
0000-0002-2915-5044
Türkiye

Enis Fuat Tüfekci
0000-0001-5051-2694
Türkiye

Mehmet Cengiz Baloğlu
0000-0003-2976-7224
Türkiye

Gokhan Zengin
0000-0001-6548-7823
Türkiye

Evren Yıldıztugay
0000-0002-4675-2027
Türkiye

Yasemin Çelik Altunoğlu ^*
0000-0003-2940-7464
Türkiye

Publication Date

December 30, 2022

Submission Date

February 23, 2022

Acceptance Date

May 9, 2022

Published in Issue

Year 2022 Volume: 25 Number: Ek Sayı 2

DOI

https://doi.org/10.18016/ksutarimdoga.v25i73358.1077985

IZ

https://izlik.org/JA63HH48CF

Cite

RIS / Bibtex

APA

Al Kateeb, A. I., Tüfekci, E. F., Baloğlu, M. C., Zengin, G., Yıldıztugay, E., & Çelik Altunoğlu, Y. (2022). Antibacterial Effects of Phlomoides molucelloides (Bunge) Salmaki. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 25(Ek Sayı 2), 402-410. https://doi.org/10.18016/ksutarimdoga.v25i73358.1077985

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