Response of Different Safflower Genotypes to Anther Culture

Dudu Büşra Nakas; Süleyman Avcı; Mehmet Demir Kaya

doi:10.18016/ksutarimdoga.vi.1084134

TR EN

Farklı Aspir Genotiplerinin Anter Kültürüne Tepkisi

Öz

Bu çalışmada; 12 aspir genotipinin artan dozlarda BAP (0, 0.5, 1 ve 2 mg L-1) ve NAA (0, 0.1 ve 0.5 mg L-1) içeren MS ortamında anter kültürüne karşı tepkileri belirlenmiştir. Donör bitkiler tarla koşullarında yetiştirilmiş ve ana çiçek tablaları erken veya geç tek çekirdekli dönemde hasat edilmiştir. Ön işlem olarak +4 °C’de ve 3-4 gün süreyle soğuk muamelesi uygulanmıştır. Aspir genotiplerinden sadece Balcı ve Linas çeşitleri rejenere olabilir kallus üretmiştir. Balcı çeşidi daha yüksek oranda kallus oluşturmasına rağmen sadece Linas çeşidinde 0.5 veya 1 mg L-1 BAP + 0.1 mg L-1 NAA kombinasyonlarından sürgün rejenerasyonu gerçekleşmiştir. Toplamda Linas çeşidine ait 8 sürgün 0.1 mg L-1 NAA içeren ½ MS ortamına aktarılmış, ancak köklenme sağlanamamıştır. Sonuç olarak; 12 aspir genotipi arasında Balcı ve Linas çeşitlerinin anter kültürü rejenerasyon potansiyelinin daha yüksek olduğu belirlenmiştir. Bununla birlikte, bu çeşitler üzerinde gelecekte sürgün geliştirme ve köklendirme çalışmalarına odaklanılması gerekmektedir.

Anahtar Kelimeler

Response of Different Safflower Genotypes to Anther Culture

Abstract

In this study; the responses of 12 safflower genotypes to anther culture were determined in MS medium containing increasing doses of BAP (0, 0.5, 1, and 2 mg L-1) and NAA (0, 0.1, and 0.5 mg L-1). Donor plants were grown under field conditions and the main capitulas were collected in early or late uninucleate stage. A pre-treatment was applied to their capitules at +4 °C for 3-4 days. Only cvs. Balcı and Linas produced regenerable callus among these genotypes. Although regenerable callus rate was higher in Balcı than Linas, shoot regeneration was achieved only in Linas with 0.5 or 1 mg L-1 of BAP + 0.1 mg L-1 of NAA combinations. In total, 8 shoots of Linas were moved to ½ MS medium consisting of 0.1 mg L-1 of NAA, but rooting of them could not be performed. In sum, among the 12 safflower genotypes, cvs. Balcı and Linas have higher anther culture regeneration potential; however, it is necessary to focus on shoot development and rooting on these cultivars in further experiments.

Keywords

Supporting Institution

Eskişehir Osmangazi University Scientific Research Projects Commission

Project Number

202023029

Thanks

This study was provided by Eskişehir Osmangazi University Scientific Research Projects Commission (Project code: 202023029). In addition, this study was prepared by using of the master thesis of Dudu Büşra Duru.

References

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Dudu Büşra Nakas
0000-0002-0717-3110
Türkiye

Süleyman Avcı ^*
0000-0002-4653-5567
Türkiye

Mehmet Demir Kaya
0000-0002-4681-2464
Türkiye

Publication Date

April 30, 2023

Submission Date

March 7, 2022

Acceptance Date

August 24, 2022

Published in Issue

Year 2023 Volume: 26 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1084134

IZ

https://izlik.org/JA65HL26UU

Cite

RIS / Bibtex

APA

Nakas, D. B., Avcı, S., & Kaya, M. D. (2023). Response of Different Safflower Genotypes to Anther Culture. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(2), 293-298. https://doi.org/10.18016/ksutarimdoga.vi.1084134

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