Phylogenetic Relationships of The Trifolium L. Species Based on cpDNA Sequences

Aykut Yılmaz; Yudum Yeltekin

doi:10.18016/ksutarimdoga.vi.1095219

TR EN

cpDNA Sekansları Temelinde Trifolium L. Türlerinin Filogenetik İlişkileri

Öz

DNA barkodlama; tür çeşitliliğinin tayininde, filogenetik ilişkilerin değerlendirilmesinde ve yetersiz ayırt edici karakterlerden dolayı taksonomik açıdan problemli türlerin kimliklendirilmesinde önemli bir moleküler yaklaşımdır. Geniş coğrafik bölgelere yayılan ve buna bağlı yüksek varyasyon gösteren Trifolium gibi türlerde, filogenetik ilişkilerin değerlendirilmesinde en iyi sonucu verecek barkodlama bölgelerini tayin etmek büyük bir öneme sahiptir. Bu amaçla, cpDNA’ya (chloroplast DNA) ait matK (maturase K) ve rbcL (ribulose bisphosphate carboxylase gen) bölgeleri Trifolium cinsinin taksonomisini değerlendirmek ve barkodlama yeteneğini tayin etmek için kullanıldı. Bu çalışmada matK bölgesinden 63 taxa ve rbcL bölgesinden 47 taxa belirlendi ve kullanıldı. Transisyonel değişimlerin, matK ve rbcL bölgeleri için transversiyonel değişimlerden sırasıyla % 51.52 ve % 70.69 ile daha yüksek olduğu gözlemlendi. Heriki barkodlama bölgesinin taksonomik olarak türleri gruplama yeteneklerinin yanısıra, filogenetik ilişkileri ortaya çıkarmak adına değerli olduğu ifade edilebilir. Bununla birlikte, özellikle bütün taksonlar için açık bir şekilde gruplama yeteneğine ve yüksek oranda değişken bölgelere sahip (158) matK sekans bilgilerinin kullanımı tavsiye edilir.

Anahtar Kelimeler

Phylogenetic Relationships of The Trifolium L. Species Based on cpDNA Sequences

Abstract

DNA barcoding is an important molecular approach in the determination of species diversity, evaluation of phylogenetic relationships and identification of taxonomically problematic species because of insufficient diagnostic characters. It has great importance to determine the barcoding regions that will give the best result in the evaluation of phylogenetic relationships in species like Trifolium that spread over wide geographical regions in the world and show high variation accordingly. For this aim, matK (maturase K) and rbcL (ribulose bisphosphate carboxylase gene) regions belonging to cpDNA(chloroplast DNA) was used to determine the barcoding ability and evaluate the taxonomy of the genus Trifolium. 63 taxa from matK region and 47 taxa from rbcL region were determined and used in this study. It was observed that transitional substitutions for matK and rbcL regions are higher than transversional substitutions with the rate of 51.52 % and 70.69 %, respectively. It can be stated that both of barcoding regions are valuable to reveal the phylogenetic relationships, in addition to their grouping ability the species as taxonomically. However, especially the using of matK sequence informations that have high variable sites (158) and grouping ability clearly for all taxa is strongly recommended.

Keywords

Thanks

The author would like to thank to NCBI for sequence informations used in this study and the authors sharing the sequence informations in NCBI.

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Aykut Yılmaz ^*
0000-0002-0327-8388
Türkiye

Yudum Yeltekin
0000-0003-3891-8717
Türkiye

Publication Date

April 30, 2023

Submission Date

March 29, 2022

Acceptance Date

August 14, 2022

Published in Issue

Year 2023 Volume: 26 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1095219

IZ

https://izlik.org/JA26CK59TE

Cite

RIS / Bibtex

APA

Yılmaz, A., & Yeltekin, Y. (2023). Phylogenetic Relationships of The Trifolium L. Species Based on cpDNA Sequences. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(2), 299-306. https://doi.org/10.18016/ksutarimdoga.vi.1095219

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