‘Skualen’ Triterpeninin Somatik Mutasyonlar Üzerine Etkisinin Drosophila melanogaster’de In vivo Kanat Benek Testi ile Araştırılması

Handan Uysal; Hatice Çelik

doi:10.18016/ksutarimdoga.vi.1103555

EN TR

Investigation of the Effect of ‘Squalene’ Triterpene on Somatic Mutations in Drosophila melanogaster by In vivo Wing Spot Test

Abstract

Squalene (SKU) terpene, which belongs to the triterpenes group of secondary metabolites and is synthesized by plants, is frequently used in different fields such as cosmetics, medicine and food industry due to its antioxidant, anti-inflammatory, antibacterial and anticarcinogenic properties. SKU, are also preferred in alternative medicine methods because of their bioactivity. In this study, the possible genotoxic effects of SKU, which is used in many different areas and taken into the body by contact or oral route, were investigated depending on the dose-time interaction. Wild and mutant strains of Drosophila melanogaster, an important model organism, are used in research in the field of genetics. The genotoxic effect of SKU was determined by somatic mutation and recombination test (SMART) and multiple wing hair (mwh) and flare (flr3) mutant strains of Drosophila melanogaster were used. For this purpose, the analysis of the data of the application groups containing different concentrations of SKU (50, 100, 200 and 400 ppm) was statistically evaluated by comparing them with the dimethylsulfoxide (DMSO) negative control group. According to the data obtained from the SMART result; while the clone induction frequency was 0.57 in the DMSO control group, this value was 0.41 for 50 ppm depending on the dose increase; 0.52 for 100 ppm; 0.93 for 200 ppm and 1.30 for 400 ppm. This increase in clone induction frequency can be considered as an indication that SKU induces somatic mutations and exhibits genotoxic effects.

Keywords

‘Skualen’ Triterpeninin Somatik Mutasyonlar Üzerine Etkisinin Drosophila melanogaster’de In vivo Kanat Benek Testi ile Araştırılması

Öz

Sekonder metabolitlerin triterpenler grubuna ait olan ve bitkiler tarafından sentezlenen skualen (SKU) terpeni, antioksidan, antienflamatuar, antibakteriyel ve antikanserojen özellikleri nedeniyle kozmetik, ilaç ve gıda endüstrisi gibi farklı alanlarda sıklıkla kullanılmaktadır. SKU, alternatif tıp yöntemlerinde de sahip oldukları bioaktiviteler nedeniyle tercih edilmektedir. Bu çalışmada, farklı birçok alanda kullanılan ve temas ya da oral yollarla vücuda alınan SKU’nun doz-süre etkileşimine bağlı olarak olası genotoksik etkileri araştırılmıştır. Genetik alanında yapılan araştırmalarda önemli bir model organizma olan Drosophila melanogaster’in yabanıl ve mutant soyları kullanılmaktadır. SKU’nun genotoksik etkisi somatik mutasyon ve rekombinasyon testi (SMART) ile belirlenmiş ve Drosophila melanogaster’in multiple wing hair (mwh) ve flare (flr3) mutant soyları kullanılmıştır. Bu amaçla SKU’nun farklı konsantrasyonlarını (50, 100, 200 ve 400 ppm) içeren uygulama gruplarına ait verilerin analizi dimetilsülfoksit (DMSO) negatif kontrol grubu ile karşılaştırılarak istatistiki olarak değerlendirilmiştir. SMART sonucundan elde edilen verilere göre; klon indüksiyon frekansı DMSO kontrol grubunda 0.57 iken bu değer doz artışına bağlı olarak 50 ppm için 0.41; 100 ppm için 0.52; 200 ppm için 0.93 ve 400 ppm içinde 1.30 olarak belirlenmiştir. Klon indüksiyon frekansındaki bu artış SKU’nun somatik mutasyonları uyardığı ve genotoksik etki sergilediğinin göstergesi olarak kabul edilebilir.

Anahtar Kelimeler

Supporting Institution

Atatürk Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi

Project Number

FHD-2019-7000

Thanks

Bu çalışma Atatürk Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi -FHD-2019-7000- kodu ile desteklenen Hatice ÇELİK’in “Drosophila Melanogaster’de Doz-Süre Etkileşimine Bağlı Olarak Terpenlerin In Vivo Toksik ve Genotoksik Etkilerinin Belirlenmesi” adlı Yüksek Lisans Tez Projesi olup, tezin gerçekleştirilmesine maddi destek sağlayan Atatürk Üniversitesi BAP birimine katkılarından dolayı teşekkür ederiz.

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Details

Primary Language

Turkish

Subjects

Structural Biology

Journal Section

Research Article

Authors

Handan Uysal ^*
0000-0002-4290-8223
Türkiye

Hatice Çelik
0000-0003-2531-6020
Türkiye

Publication Date

June 30, 2023

Submission Date

April 14, 2022

Acceptance Date

October 20, 2022

Published in Issue

Year 2023 Volume: 26 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1103555

IZ

https://izlik.org/JA56FA62RK

Cite

RIS / Bibtex

APA

Uysal, H., & Çelik, H. (2023). ‘Skualen’ Triterpeninin Somatik Mutasyonlar Üzerine Etkisinin Drosophila melanogaster’de In vivo Kanat Benek Testi ile Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(3), 477-486. https://doi.org/10.18016/ksutarimdoga.vi.1103555

‘Skualen’ Triterpeninin Somatik Mutasyonlar Üzerine Etkisinin Drosophila melanogaster’de In vivo Kanat Benek Testi ile Araştırılması

Investigation of the Effect of ‘Squalene’ Triterpene on Somatic Mutations in Drosophila melanogaster by In vivo Wing Spot Test

Abstract

Keywords

‘Skualen’ Triterpeninin Somatik Mutasyonlar Üzerine Etkisinin Drosophila melanogaster’de In vivo Kanat Benek Testi ile Araştırılması

Öz

Anahtar Kelimeler

Supporting Institution

Project Number

Thanks

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Cited By

Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster

Antigenotoxic Effects of Stachys viscosa (Lamiaceae), a potential medicinal plant in Drosophila melanogaster

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