Evaluation of Agronomic Traits and Allele Specific DNA Markers Related to Some Disease and Quality Traits in Mutant Karakılçık M4 Individuals

İlker Yüce; Ziya Dumlupınar

doi:10.18016/ksutarimdoga.vi.1120224

EN TR

Evaluation of Agronomic Traits and Allele Specific DNA Markers Related to Some Disease and Quality Traits in Mutant Karakılçık M4 Individuals

Abstract

Karakılçık (KK) is a landrace, of which both bread and durum wheat forms exist. This is the first study that reports mutation induction and detection with agronomic traits and allele specific markers on KK durum wheat landrace. In the study, KK landrace was induced with chemical mutation using sodium azide (NaN3) (3 mM) mutagen to improve agronomic traits. In the research, KK genotype and the 13 M4 individuals (KK-1 to KK-13) were used as plant materials. According to the results, the shortest mutant genotype was KK-10 (125.80 cm), with the highest protein ratio (18.50%) and wet gluten ratio (37.10%), while KK-9 genotype had the highest grain yield (4285.6 kg ha-1). The average polymorphism information content (PIC) was calculated as 0.97, while the average allele number was 15.2 per marker. Glu-B1 (Bx7OE primer) allele was determined on KK-11 and KK-13 genotypes. Wx-A1 allele was found on KK-1, KK-2, KK-4, KK-5 and KK-7 genotypes. Sr49 allele (Sun209) was determined on KK-5 genotype, while Yr45 allele (Xwgp118) was detected on KK-10 genotype. In addition, the high protein content allele Gpc-B1 (UHW89 primer) was found on KK-1, KK-2, KK-3 and KK-4 genotypes. Based on the principal component biplot analysis, it was determined that there was a positive relationship between grain yield (GY) with test weight (TW), grain number and weight per spike (GNS and GWS), and negative relationship with the other traits.

Keywords

Mutant Karakılçık M4 Bireylerinde Agronomik Özellikler, Bazı Hastalık ve Kalite Özellikleri ile İlişkili Allellerin DNA Markörleri ile Saptanması

Öz

Karakılçık (KK), hem ekmeklik hem de makarnalık buğday formları bulunan yerel bir çeşittir. Bu, yerel KK makarnalık buğday çeşidinde agronomik özellikler ve allel spesifik markörler ile mutasyon tespitini bildiren ilk çalışmadır. Çalışmada, agronomik özellikleri iyileştirmek için sodyum azid (NaN3) (3 mM) mutajeni kullanılarak kimyasal mutasyon ile yerel KK çeşidi muamele edilmiştir. Araştırmada bitki materyali olarak KK genotipi ve 13 M4 bireyi (KK-1 ila KK-13) kullanılmıştır. Elde edilen sonuçlara göre, en yüksek protein oranı (%18.50), yaş gluten oranı (%37.10) ile en kısa mutant genotip KK-10 (125.80 cm) olurken, KK-9 en yüksek tane verimine (4285.6 kg ha-1) sahip genotip olmuştur. Ortalama polimorfizm bilgi içeriği (PIC) 0.97, ortalama allel sayısı markör başına 15.2 olarak hesaplanmıştır. Glu-B1 (Bx7OE) alleli, KK-11 ve KK-13 genotiplerinde, Wx-A1 alleli ise KK-1, KK-2, KK-4, KK-5 ve KK-7 genotiplerinde belirlenmiştir. KK-5 genotipinde Sr49 alleli (Sun209), KK-10 genotipinde ise Yr45 alleli (Xwgp118) tespit edilmiştir. Ayrıca KK-1, KK-2, KK-3 ve KK-4 genotiplerinde yüksek protein içerikli allel olan Gpc-B1 alleli (UHW89) bulunmuştur. Temel bileşen biplot analizine göre, tane verimi (TV) ile hektolitre ağırlığı (HL), başakta tane sayısı ve başakta tane ağırlığı (BTS ve BTA) arasında pozitif, diğer özelliklerle negatif ilişki olduğu belirlenmiştir.

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

İlker Yüce
0000-0002-9761-3561
Türkiye

Ziya Dumlupınar ^*
0000-0003-3119-6926
Türkiye

Early Pub Date

May 15, 2023

Publication Date

August 31, 2023

Submission Date

May 23, 2022

Acceptance Date

February 27, 2023

Published in Issue

Year 2023 Volume: 26 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1120224

IZ

https://izlik.org/JA43LJ86BK

Cite

RIS / Bibtex

APA

Yüce, İ., & Dumlupınar, Z. (2023). Evaluation of Agronomic Traits and Allele Specific DNA Markers Related to Some Disease and Quality Traits in Mutant Karakılçık M4 Individuals. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(4), 861-869. https://doi.org/10.18016/ksutarimdoga.vi.1120224

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