Determination of in Vitro Antioxidant, Antimicrobial Properties and COX-1/COX-2 Enzyme Inhibition Activity of Capparis Sicula

Nağihan Acar; Fatih Çağlar Çelikezen; İbrahim Halil Şahin; Mehmet Fırat; Recep Koçyiğit; Oğuz Ayhan Kireçci

doi:10.18016/ksutarimdoga.vi.1139197

TR EN

Capparis sicula'nın in vitro Antioksidan, Antimikrobiyal Özellikleri ve COX-1/COX-2 Enzim Inhibisyon Aktivitesinin Belirlenmesi

Öz

Sentetik ilaçlar birçok yan etkiye ve yüksek maliyete neden olduğundan daha az yan etkisi olan ve nispeten daha ucuz olan bitkisel bazlı ilaçların geliştirilmesi artan bir ilgi görmüştür. Capparis sicula halk arasında bazı hastalıkların tedavisinde geleneksel olarak kullanılmaktadır. Bu amaçla sunulan çalışmada Capparis sicula bitkisinin metanol ekstraktının antioksidan ve antimikrobiyal özellikleri ile COX-1 ve COX-2 enzimleri üzerindeki inhibisyon etkileri araştırılmıştır. Çalışmada Capparis sicula bitkisinin antioksidan özellikleri DPPH ve CUPRAC yöntemleri ile belirlenirken, antimikrobiyal özellikleri disk difüzyon yöntemi ile belirlenmiştir. Capparis sicula'nın COX-1 ve COX-2 enzimleri üzerindeki etkisi ise, ticari kitler kullanılarak kolorimetrik olarak belirlendi. Sonuçlar, Capparis sicula'nın önemli bir antioksidan etkiye sahip olduğunu, ancak standart Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli ve Candida albicans suşları üzerinde herhangi bir antimikrobiyal etkiye sahip olmadığını gösterdi. Ayrıca COX-1 enzimi üzerindeki inhibisyon etkisi ilk kez %4.23, COX-2 enzimi üzerindeki inhibisyon etkisi ise %23.21 olarak tespit edilmiştir. Sonuç olarak, ilaç, gıda ve kozmetik endüstrileri, Capparis sicula'yı önemli bir doğal hammadde kaynağı olarak kullanabilir.

Anahtar Kelimeler

Determination of in Vitro Antioxidant, Antimicrobial Properties and COX-1/COX-2 Enzyme Inhibition Activity of Capparis Sicula

Abstract

Since synthetic drugs cause many side effects and have a high cost, there has been increasing interest in the development of herbal-based drugs that have fewer side effects and are relatively inexpensive. Capparis sicula is traditionally used in the treatment of some diseases among people. For this purpose, the antioxidant and antimicrobial properties of the methanol extract of the Capparis sicula plant and its inhibitory effects on COX-1 and COX-2 enzymes were investigated. In the study, the antioxidant properties of the Capparis sicula plant were determined by DPPH and CUPRAC methods, while its antimicrobial properties were determined by the disk diffusion method. The effect of Capparis sicula on COX-1 and COX-2 enzymes was determined colorimetrically using commercial kits. The results showed that Capparis sicula had a significant antioxidant effect, but did not have any antimicrobial effect on standard strains of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans. In addition, the inhibitory effect on the COX-1 enzyme was 4.23% for the first time, and the inhibition effect on the COX-2 enzyme was determined as 23.21%. As a result, the pharmaceutical, food and cosmetic industries can use Capparis sicula as an important source of natural raw materials.

Keywords

Supporting Institution

Bitlis Eren Üniversitesi BAP

Project Number

2020.07

Thanks

This study was produced from Nagihan ACAR's master's thesis with the same name. The present study was supported by Bitlis Eren University Scientific Research Projects Coordinatorship as project numbered BEBAP 2020.07.

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Details

Primary Language

English

Subjects

Plant Biochemistry

Journal Section

Research Article

Authors

Nağihan Acar
0000-0002-8186-9925
Türkiye

Fatih Çağlar Çelikezen
0000-0001-5489-7384
Türkiye

İbrahim Halil Şahin
0000-0001-7667-9310
Türkiye

Mehmet Fırat
0000-0001-5814-614X
Türkiye

Recep Koçyiğit
0000-0002-0472-743X
Türkiye

Oğuz Ayhan Kireçci ^*
0000-0003-2205-4758
Türkiye

Early Pub Date

June 14, 2023

Publication Date

December 31, 2023

Submission Date

July 8, 2022

Acceptance Date

March 31, 2023

Published in Issue

Year 2023 Volume: 26 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1139197

IZ

https://izlik.org/JA78ZR63PS

Cite

RIS / Bibtex

APA

Acar, N., Çelikezen, F. Ç., Şahin, İ. H., Fırat, M., Koçyiğit, R., & Kireçci, O. A. (2023). Determination of in Vitro Antioxidant, Antimicrobial Properties and COX-1/COX-2 Enzyme Inhibition Activity of Capparis Sicula. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(6), 1221-1229. https://doi.org/10.18016/ksutarimdoga.vi.1139197