Evaluation of Oxidative Stress And Growth Alterations on Arthrospira Platensis Gomont and Chlorella Vulgaris Beijerinck (Beijerinck) by Cambio

Şükrüye Er; Hatice Tunca; Ali Doğru; Tuğba Ongun Sevindik

doi:10.18016/ksutarimdoga.vi.1174954

TR EN

Cambio'nun Arthrospira Platensis Gomont ve Chlorella Vulgaris Beijerinck (Beijerinck) Üzerinde Oluşturduğu Oksidatif Stresin Ve Büyüme Değişimlerinin Değerlendirilmesi

Öz

Bu çalışma, Cambio'nun farklı konsantrasyonlarının Chlorella vulgaris (0-500 μg mL-1) ve Arthrospira platensis (0-50 μg mL-1) alglerinde oluşturduğu biyokütle (klorofil-a miktarı, OD 750) ve antioksidan parametrelerindeki (Süperoksit dismutaz (SOD), Askorbat peroksidaz (APX), glutatyon peroksidaz (GR) enzim aktiviteleri ve malondialdehit (MDA), hidrojen peroksit (H202) ve prolin içerikleri) değişimleri belirlemeyi amaçlamaktadır. A. platensis, yüksek besin içeriği nedeniyle ticari olarak kullanılan bir siyanobakteridir. C. vulgaris, biyoremediasyon kabiliyeti, ilaç hammaddesinin yapısı ve besin bileşimi nedeniyle tıbbi ve ticari amaçlar için kullanılmaktadır. Cambio, en yüksek konsantrasyonda A. platensis hücreleri için toksiktir, ancak C. vulgaris' in büyümesini uyarmıştır. Kozmopolit olan bu iki algin üzerinde yapılan ekotoksikolojik çalışmalar kimyasalların tatlı su ekosistemlerinde meydana getirdiği zararlı etkilerin belirlenmesi adına önemlidir. A. platensis uygulamasında, süperoksit dismutaz aktivitesi orta konsantrasyonlarda; Askorbat peroksidaz aktivitesi ise en yüksek konsantrasyonda anlamlı olarak azalmıştır (p<0.05). Ayrıca, Glutatyon redüktazın aktivitesi, 20 µg mL-1 konsantrasyonunda artış göstermiştir. Malondialdehit ve H2O2 miktarında önemli değişiklikler görülmemiştir. Ancak prolin içeriği, kontrole kıyasla tüm Cambio konsantrasyonlarında önemli artışlar göstermiştir (p<0.05). Ancak C. vulgaris uygulaması için antioksidan parametrelerde herhangi bir değişiklik gözlenmemiştir. Bu sonuçlar Cambio'nun A. platensis üzerindeki etkilerinin C. vulgaris üzerindeki etkilerine göre daha yıkıcı olduğunu göstermektedir.

Anahtar Kelimeler

Evaluation of Oxidative Stress And Growth Alterations on Arthrospira Platensis Gomont and Chlorella Vulgaris Beijerinck (Beijerinck) by Cambio

Abstract

This study aims to evaluate the toxicity effects of different concentrations of Cambio on Chlorella vulgaris (0-500 μg mL-1) and Arthrospira platensis (0-50 μg mL-1) algae by determining the changes in chlorophyll-a amount, OD 750 (biomass) and antioxidant parameters (the activities of Superoxide dismutase (SOD), Ascorbate peroxidase (APX), Glutathione reductase (GR) and the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2), proline). A. platensis is being a cyanobacterium used commercially because of its high nutrient content. C. vulgaris used for medical and commercial purposes due to the capability of bioremediation, the structure of drug raw material, and nutrient compound. Ecotoxicological studies on these cosmopolitan algae are important for determining the harmful effects of chemicals on freshwater ecosystems. Cambio was toxic to A. platensis cells at the highest concentration, however, it stimulated the growth of C. vulgaris. For A. platensis application, the activity of Superoxide dismutase significantly decreased at moderate concentrations (p<0.05), while the activity of Ascorbate peroxidase decreased at the highest concentration (p<0.05). Moreover, the activity of Glutathione reductase rose at 20 μg mL-1 concentration. Malondialdehyde and H2O2 did not show significant changes, but the proline content showed significant increases in all Cambio concentrations compared to the control (p<0.05). However, for C. vulgaris application the antioxidant parameters did not show any alterations. These results are indicated that the effects of Cambio on A. platensis are more destructive than C. vulgaris.

Keywords

Supporting Institution

Sakarya Üniversitesi, BAP

Project Number

2018-02- 09-173

Thanks

The authors would like to thank to Prof. Dr. Oya Işık from Çukurova University, Faculty of Fisheries for providing us C. vulgaris culture, and also, to Sakarya University Research Foundation for financially supporting this research

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Şükrüye Er ^*
0000-0002-0815-8430
Türkiye

Hatice Tunca
0000-0003-3724-5215
Türkiye

Ali Doğru
0000-0003-0060-4691
Türkiye

Tuğba Ongun Sevindik
0000-0001-7682-0142
Türkiye

Early Pub Date

May 27, 2023

Publication Date

October 31, 2023

Submission Date

September 14, 2022

Acceptance Date

March 31, 2023

Published in Issue

Year 2023 Volume: 26 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1174954

IZ

https://izlik.org/JA36HE45ZZ

Cite

RIS / Bibtex

APA

Er, Ş., Tunca, H., Doğru, A., & Ongun Sevindik, T. (2023). Evaluation of Oxidative Stress And Growth Alterations on Arthrospira Platensis Gomont and Chlorella Vulgaris Beijerinck (Beijerinck) by Cambio. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(5), 1120-1134. https://doi.org/10.18016/ksutarimdoga.vi.1174954