Van Gölü Havzası’nda Bazı Endemik ve Nadir Astragalus Taksonlarının Filogenetik İlişkilerinin Belirlenmesi

İlhan Kaya

doi:10.18016/ksutarimdoga.vi.1183518

EN TR

Determining Phylogenetic Relationships of Some Endemic and Rare Astragalus Taxa in the Van Lake BasinDetermining Phylogenetic Relationships of Some Endemic and Rare Astragalus Taxa in the Van Lake Basin

Abstract

Astragalus, the most diverse genus in the world, contains about 3000 different taxa. This genus has 489 taxa and 63 sections in Turkey, giving it a 51% endemism rate. 82 Astragalus taxa were collected from the Van Lake Basin in 2019 for this research. These species' morphological diagnoses revealed that four of them were rare and 17 of them were endemic. In this research, the universal primers ITS4 and ITS5 were used to amplify the ITS1 and ITS2 sections, which included the 5.8S gene of rDNA. Different programs (SnapGene, CLC DNA Workbench) were used to analyze the genome information of species in the genus Astragalus. The DNA sequences of Astragalus species have been uploaded to the GenBank database of the National Center for Biotechnology Information (NCBI) (Maryland, USA), which is open to all researchers worldwide. The length of the ITS in the study, including the 5.8S sequence, varied from 669 to 687 bp. The results showed that the phylogenetic tree combined with the most stable secondary (20) structure obtained from the universal ITS4 and ITS5 primers is an effective tool for the identification of Astragalus taxa.

Keywords

Van Gölü Havzası’nda Bazı Endemik ve Nadir Astragalus Taksonlarının Filogenetik İlişkilerinin Belirlenmesi

Öz

Dünyanın en çeşitli cinsi olan Astragalus, yaklaşık 3000 farklı takson içermektedir. Bu cins Türkiye'de 489 takson ve 63 bölüme sahiptir ve bu da ona %51 endemizm oranı vermektedir. Bu araştırma için 2019 yılında Van Gölü Havzası'ndan 82 Astragalus taksonu toplanmıştır. Bu türlerin morfolojik tanıları, dördünün nadir, 17'sinin endemik olduğunu ortaya koymuştur. Bu araştırmada, rDNA'nın 5.8S genini içeren ITS1 ve ITS2 bölümlerini çoğaltmak için evrensel primerler olan ITS4 ve ITS5 kullanılmıştır. Astragalus cinsindeki türlerin genom bilgilerini analiz etmek için farklı programlar (SnapGene, CLC DNA Workbench) kullanılmıştır. Astragalus türlerinin DNA dizileri, Ulusal Biyoteknoloji Bilgi Merkezi'nin (NCBI) (Maryland, USA) dünya çapında tüm araştırmacılara açık olan GenBank veri tabanına yüklenmiştir. 5.8S dizisi de dahil olmak üzere çalışmadaki ITS uzunluğu 669 ila 687 bp arasında değişmiştir. Sonuçlar, evrensel ITS4 ve ITS5 primerlerinden elde edilen en kararlı ikincil (20) yapı ile birleştirilen filogenetik ağacın, Astragalus taksonlarının tanımlanması için etkili bir araç olduğunu göstermiştir.

Anahtar Kelimeler

Supporting Institution

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırmalar Proje Başkanlığı

Project Number

FYL-2019-8085

Thanks

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırmalar Proje Başkanlığına teşşekür ederiz

References

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Details

Primary Language

Turkish

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

İlhan Kaya ^*
0000-0002-1271-4479
Türkiye

Early Pub Date

May 15, 2023

Publication Date

August 31, 2023

Submission Date

October 3, 2022

Acceptance Date

January 23, 2023

Published in Issue

Year 2023 Volume: 26 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1183518

IZ

https://izlik.org/JA67US88KT

Cite

RIS / Bibtex

APA

Kaya, İ. (2023). Van Gölü Havzası’nda Bazı Endemik ve Nadir Astragalus Taksonlarının Filogenetik İlişkilerinin Belirlenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(4), 806-814. https://doi.org/10.18016/ksutarimdoga.vi.1183518