Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro.

Semih Dalkılıç; Lütfiye Kadıoğlu Dalkılıç; Songül Fidan; Mustafa Timurkaan; Lütfü Uygur; Gökhan Akay; İsmail Korkmaz

doi:10.18016/ksutarimdoga.vi.1192081

TR EN

Mentha pulegium ekstraktı güçlü in vitro antimikrobiyal ve sitotoksik etki göstermiştir

Öz

Mentha pulegium tıbbi açıdan önemli ve iyi bilinen bir bitkidir ve tıbbi, beslenme ve baharat gibi çeşitli amaçlar için kullanılır. Bu çalışmada M. pulegium ekstraktının antimikrobiyal, antioksidan ve sitotoksik özelliklerini görmek için analiz edildi. M. pulegium'un antimikrobiyal aktivitesi agar kuyucuk yöntemi kullanılarak test edildi. MIC, MBC ve antimikrobiyal aktivite Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25322), Bacillus megaterium (DSM32) ve Candida albicans (FMC17) mikroorganizmaları üzerinde test edildi. Pozitif kontrol olarak Klindamisin (2 µg) ve Amoksisilin (30 µg), negatif kontrol olarak Dimetilsülfoksit (DMSO) kullanıldı. 3-(4,5-dimetiltiyazol-2-il) -2,5-difenil tetrazolyum bromür (MTT) test yöntemi kullanılarak aseton, kloroform ve metanol gibi çözücülerden elde edilen farklı konsantrasyonlarda ekstraktların sitotoksik aktivitesi; ekstraktların farklı konsantrasyonlarına maruz kaldıktan sonra µg/ml sitotoksik aktivite ve hücre ölüm şekilleri; Aseton, kloroform ve metanol gibi çözücülerden 3-(4,5-dimetiltiyazol-2-il) -2,5-difenil tetrazolyum bromür (MTT) test yöntemi kullanılarak aseton, kloroform ve metanol insan meme kanseri (MDA-MB-231), insan pankreas kanseri (PANC1), insan yumurtalık kanseri (OVCAR3) ve insan akciğer kanseri (A549) hücre hatları buna göre belirlendi. Sonuç olarak M. pulegium ekstraktının güçlü bir antimikrobiyal aktivite spektrumuna ve sitotoksik etkiye sahip olduğu belirlenmiştir.

Anahtar Kelimeler

Supporting Institution

TÜBİTAK

Project Number

1919B012001186

Thanks

Thanks to Prof. Dr. Mustafa Kaplan, Fırat University Faculty of Medicine Department of Parasitology, for contributing to the laboratory environment in the realization of this study.

References

1. Awouafack MD, McGaw LJ, Gottfried S, Mbouangouere R, Tane P, Spiteller M, et al. Antimicrobial activity and cytotoxicity of the ethanol extract, fractions and eight compounds isolated from Eriosema robustum (Fabaceae). BMC Complementary and Alternative Medicine. 2013;13(1):1-9. https://doi.org/10.1186/1472-6882-13-289
2. Ahmed A, Ayoub K, Chaima AJ, Hanaa L, Abdelaziz C. Effect of drying methods on yield, chemical composition and bioactivities of essential oil obtained from Moroccan Mentha pulegium L. Biocatalysis and Agricultural Biotechnology. 2018;16:638-643. https://doi.org/10.1016/j.bcab.2018.10.016
3. Andersson DI, Hughes D. Persistence of antibiotic resistance in bacterial populations. FEMS Microbiology Reviews. 2011;35(5):901-911. https://doi.org/10.1111/j.1574-6976.2011.00289.x
4. Benlarbi KH, Elmtili N, Macías FA, Galindo JCG. Influence of in vitro growth conditions in the production of defence compounds in Mentha pulegium L. Phytochemistry Letters. 2014;8:233-244. https://doi.org/10.1016/j.phytol.2014.03.007
5. Buys SS, Partridge E, Greene MH, Prorok PC, Reding D, Riley TL, Hartge P, Fagerstrom RM, Ragard LR, Chia D, Izmirlian G, Fouad M, Johnson CC, Gohagan JK, PLCO Project Team. Ovarian cancer screening in the Prostate, Lung, Colorectal and Ovarian (PLCO) cancer screening trial: findings from the initial screen of a randomized trial. American Journal of Obstetrics and Gynecology. 2005;193(5):1630–1639. https://doi.org/10.1016/j.ajog.2005.05.005
6. Chalchat JC, Gorunovic MS, Maksimovic ZA, Petrovic SD. Essential oil of wild growing Mentha Pulegium L. from Yugoslavia. Journal of Essential Oil Research. 2000;12(5):598-600. https://doi.org/10.1080/10412905.2000.9712166
7. Cevatemre B. Fenretinid ve indol-3-karbinol kombinasyonunun meme kanseri hücre soyları üzerindeki sitotoksik/apoptotik etkilerinin araştırılması [Master's thesis]. Bursa: Uludağ Üniversitesi; 2012. Türkçe.
8. Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 1999;12(4):564–582. https://doi.org/10.1128/CMR.12.4.564

9. Coşkun MD. Nf-kb ve oksidatif stresin inhibisyonu ile peripheral nöropatinin iyileştirilmesi ve kanser kemoterapi sonuçlarının geliştirilmesi [Doctoral thesis]. Bursa: Uludağ Üniversitesi; 2017.Türkçe.
10. Caceres A, Cano O, Samayoa B, Aguilar L. Plants used in guatemala for the treatment of gastrointestinal disorders. 1. Screening of 84 plants against enterobacteria. Journal of Ethnopharmacology. 1990;30(1):55–73. https://doi.org/10.1016/0378-8741(90)90017-n
11. Çöteli E, Erden Y, Karataş F. Yarpuz (Mentha Pulegium L.) bitkisindeki malondialdehit, glutatyon ve vitamin miktarları ile total antioksidan kapasitesinin araştırılması. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2013;17(2):4-10. Türkçe. https://dergipark.org.tr/en/pub/sdufenbed/issue/20801/222003
12. Dorman HJD, Koşar M, Kahlos K, Holm Y, Hiltunen R. Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. Journal of Agricultural and Food Chemistry. 2003;51(16):4563–4569. https://doi.org/10.1021/jf034108k
13. Dalkılıç S, Dalkılıç LK, Korkmaz İ. Geleneksel kahvaltılık zahterin antimikrobiyal etkisi. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2020;10(1):128-133. Türkçe. https://doi.org/10.17714/gumusfenbil.579489 14. Duru ME, Oztürk M, Uğur A, Ceylan O. The constituents of essential oil and in vitro antimicrobial activity of Micromeria cilicica from Turkey. Journal of Ethnopharmacology. 2000;94(1):43–48. https://doi.org/10.1016/j.jep.2004.03.053
15. Dutta D, Chakraborty A, Mukherjee B, Gupta S. Aptamer-conjugated apigenin nanoparticles to target colorectal carcinoma: a promising safe alternative of colorectal cancer chemotherapy. ACS Applied Bio Materials. 2018;1(5):1538-1556. https://doi.org/10.1021/acsabm.8b00441
16. Erarslan ZB, Ecevit Genç G, Kültür Ş. Medicinal plants traditionally used to treat skin diseases in Turkey–eczema, psoriasis, vitiligo. Journal of Faculty of Pharmacy of Ankara University. 2020;44(1):137-166. https://doi.org/10.33483/jfpau.586114
17. Erdogan EA, Everest A, Kaplan E. Antimicrobial activities of aqueous extracts and essential oils of two endemic species from Turkey. Indian Journal of Traditional Knowledge. 2013;12(2):221-4.
18. Ebana RUB, Etim OE, Edet UO, John RS, Osom AO. Comparative evaluation of phytochemicals and antibacterial activity of the roots and leaves of anthocleista vogelii on some clinical ısolates. Asian Journal of Research in Botany. 2018;1(1):1-8. https://journalajrib.com/index.php/AJRIB/article/view/1118
19. Faydaoğlu E, Sürücüoğlu M. Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidan aktiviteleri ve kullanım olanakları. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2013;6(2):233-265. Türkçe. https://dergipark.org.tr/en/pub/erzifbed/issue/6027/80707
20. Greenwell M, Rahman PKSM. Medicinal plants: their use in anticancer treatment. International Journal of Pharmaceutical Sciences And Research. 2015;6(10):4103. https://dx.doi.org/10.13040%2FIJPSR.0975-8232.6(10).4103-12
21. González-Tejero MR, Casares-Porcel M, Sánchez-Rojas CP, Ramiro-Gutiérrez JM, Molero-Mesa J, Pieroni A, et al. Medicinal plants in the Mediterranean area: synthesis of the results of the project Rubia. Journal of Ethnopharmacology. 2008; 116(2):341–357. https://doi.org/10.1016/j.jep.2007.11.045
22. Gülçin İ, Gören AC, Taslimi P, Alwasel SH, Kılıc O, Bursal E. Anticholinergic, antidiabetic and antioxidant activities of Anatolian pennyroyal (Mentha pulegium)-analysis of its polyphenol contents by LC-MS/MS. Biocatalysis and Agricultural Biotechnology. 2020;23:101441. https://doi.org/10.1016/j.bcab.2019.101441
23. Hassan E, Ullah H. Antibacterial and antifungal activities of the medicinal plant veronica biloba. Journal of Chemistry. 2019. https://doi.org/10.1155/2019/5264943
24. Hesham HM, Lasheen DS, Abouzid KA. Chimeric HDAC inhibitors: Comprehensive review on the HDAC-based strategies developed to combat cancer. Medicinal Research Reviews. 2018;38(6):2058-2109. https://doi.org/10.1002/med.21505
25. Hemeg HA, Moussa IM, Ibrahim S, Dawoud TM, Alhaji JH, Mubarak AS, et al. Antimicrobial effect of different herbal plant extracts against different microbial population. Saudi Journal of Biological Sciences. 2020; 27(12):3221–3227. https://doi.org/10.1016/j.sjbs.2020.08.015
26. . Kurt H, Keşkek ŞÖ, Çil T, Canataroğlu A. Meme kanserli hastalarda tamamlayıcı/alternatif tedavi kullanımı. Türk Onkoloji Dergisi. 2013; 28(1):10-15. Türkçe. https://doi.org/10.5505/tjoncol.2013.827
27. Lowy FD. Antimicrobial resistance: the example of Staphylococcus aureus. The Journal of Clinical Investigation. 2003;111(9):1265-1273. https://doi.org/10.1172/JCI18535
28. Liu Q, Meng X, Li Y, Zhao CN, Tang GY, Li HB. Antibacterial and antifungal activities of spices. International Journal of Molecular Sciences. 2017;18(6):1283. https://doi.org/10.3390/ijms18061283
29. Liu J, Chen H, Miller DS, Saavedra JE, Keefer LK, Johnson DR, Klaassen CD, Waalkes MP. Overexpression of glutathione S-transferase II and multidrug resistance transport proteins is associated with acquired tolerance to inorganic arsenic. Molecular Pharmacology. 2001;60(2):302–309. https://doi.org/10.1124/mol.60.2.302
30. McGaw LJ, Eloff JN. Ethnoveterinary use of southern African plants and scientific evaluation of their medicinal properties. Journal of Ethnopharmacology. 2008;119(3):559-574. https://doi.org/10.1016/j.jep.2008.06.013
31. Mamadalieva NZ, Hussain H, Xiao J. Recent advances in genus Mentha: Phytochemistry, antimicrobial effects, and food applications. Food Frontiers. 2020;1(4):435-458. https://doi.org/10.1002/fft2.53
32. Marzouk B, Fredj MBH, Chraief I, Mastouri M, Boukef K, Marzouk Z. Chemical composition and antimicrobial activity of essential oils from tunisian Mentha pulegium L. Journal of Food Agriculture and Environment. 2008;6(1):78
33. Mensor LL, Menezes FS, Leitão GG, Reis AS, Santos TC, Coube CS, Leitão SG. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research. 2001;15(2):127–130. https://doi.org/10.1002/ptr.687
34. Nascimento GGF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology. 2000;31(4):247-256. https://doi.org/10.1590/S1517-83822000000400003
35. Pucci C, Martinelli C, Ciofani G. Innovative approaches for cancer treatment: current perspectives and new challenges. Ecancer Medical Science. 2019;13:961. https://dx.doi.org/10.3332%2Fecancer.2019.961
36. Sofowora A, Ogunbodede E, Onayade A. The role and place of medicinal plants in the strategies for disease prevention. African Journal of Traditional, Complementary and Alternative Medicines. 2013;10(5):210-229. https://doi.org/10.4314/ajtcam.v10i5.2
37. Sakagami Y, Kajimura K. Bactericidal activities of disinfectants against vancomycin-resistant enterococci. The Journal of Hospital İnfection. 2002;50(2):140–144. https://doi.org/10.1053/jhin.2001.1150
38. Swamy MK, Akhtar MS, Sinniah UR. Antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review. Evidence-Based Complementary and Alternative Medicine. 2016. https://doi.org/10.1155/2016/3012462
39. Salem N, Bachrouch O, Sriti J, Msaada K, Khammassi S, Hammami M, Selmi S, Boushih E, Koorani S, Abderraba M, Marzouk B, Limam F. Fumigant and repellent potentials of Ricinus communis and Mentha pulegium essential oils against Tribolium castaneum and Lasioderma serricorne. International Journal of Food Properties. 2017;20:2899-2913. https://doi.org/10.1080/10942912.2017.1382508
40. Stockert JC, Blázquez-Castro A, Cañete M, Horobin RW, Villanueva A. MTT assay for cell viability: Intracellular localization of the formazan product is in lipid droplets. Acta Histochemica. 2012;114(8):785–796. https://doi.org/10.1016/j.acthis.2012.01.006
41. Soylu EN, Çebi A. Scenedesmus sp.’nin DPPH (1, 1-difenil-2-pikrilhidrazil) serbest radikal süpürücü aktivitesi. Karadeniz Fen Bilimleri Dergisi. 2017;7(2):146-151. Türkçe. https://doi.org/10.31466/kfbd.353777
42. Sharma OP, Bhat TK. DPPH antioxidant assay revisited. Food Chemistry. 2009;113(4):1202-1205. https://doi.org/10.1016/j.foodchem.2008.08.008
43. Shoeb M. Anti-cancer agents from medicinal plants. Bangladesh Journal of Pharmacology. 2006;1(2):35-41. https://doi.org/10.3329/bjp.v1i2.486
44. Şekerli M, Kılıç N, Duman DC. Liken metabolitlerinin antikanser aktivite etkisinin moleküler düzeydeki mekanizmaları. Türk Hijyen ve Deneysel Biyoloji Dergisi. 2017;74(1): 95-102. Türkçe. https://dx.doi.org/10.5505/TurkHijyen.2016.24650
45. Top R, Erden Y, Tekin S. Bazı önemli tıbbi bitkilerin antioksidan ve antikanser etkilerinin araştırılması. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi. 2019;8(2):435-442. Türkçe. https://doi.org/10.17798/bitlisfen.485413
46. Talbaoui A, Aneb M, Bouyahya A, Boury HE, Amzazi S, Benjouad A, et al. In vitro cytotoxic effects and antibacterial activity of Moroccan medicinal plants Aristolochia longa and Lavandula multifida. European Journal of Medicinal Plants. 2016;16(2): 1-13. http://dx.doi.org/10.9734/ejmp
47. Tariq A, Sadia S, Pan K, Ullah I, Mussarat S, Sun F. A systematic review on ethnomedicines of anti-cancer plants. Phytotherapy Research. 2017;31(2):202–264. https://doi.org/10.1002/ptr.5751
48. Ugboko HU, Nwinyi OC, Oranusi SU, Fatoki TH, Omonhinmin A. Antimicrobial importance of medicinal plants in Nigeria. The Scientific World Journal. 2020. https://doi.org/10.1155/2020/7059323
49. Wińska K, Mączka W, Łyczko J, Grabarczyk M, Czubaszek A, Szumny A. Essential oils as antimicrobial agents-myth or real alternative. Molecules. 2019; 24(11):2130. https://doi.org/10.3390/molecules24112130
50. . Wink M. Evolution of secondary metabolites from an ecological and molecular phylogenetic perspective. Phytochemistry. 2003;64(1):3–19. https://doi.org/10.1016/s0031-9422(03)00300-5
51. Woopen H, Sehouli J. Current and future options in the treatment of malignant ascites in ovarian cancer. Anticancer Research. 2009;29(8):3353–3359.

Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Semih Dalkılıç ^*
0000-0002-6892-247X
Türkiye

Lütfiye Kadıoğlu Dalkılıç
0000-0002-6791-3811
Türkiye

Songül Fidan
0000-0003-1876-858X
Türkiye

Mustafa Timurkaan
0000-0003-1950-0489
Türkiye

Lütfü Uygur
0000-0002-5113-2703
Türkiye

Gökhan Akay
0000-0003-3892-2572
Türkiye

İsmail Korkmaz
0000-0003-4631-7786
Türkiye

Early Pub Date

June 14, 2023

Publication Date

December 31, 2023

Submission Date

October 20, 2022

Acceptance Date

February 27, 2023

Published in Issue

Year 1970 Volume: 26 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1192081

IZ

https://izlik.org/JA27GR67UZ

Cite

RIS / Bibtex

APA

Dalkılıç, S., Kadıoğlu Dalkılıç, L., Fidan, S., Timurkaan, M., Uygur, L., Akay, G., & Korkmaz, İ. (2023). Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(6), 1291-1304. https://doi.org/10.18016/ksutarimdoga.vi.1192081