Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro.

Semih Dalkılıç; Lütfiye Kadıoğlu Dalkılıç; Songül Fidan; Mustafa Timurkaan; Lütfü Uygur; Gökhan Akay; İsmail Korkmaz

doi:10.18016/ksutarimdoga.vi.1192081

TR EN

Mentha pulegium ekstraktı güçlü in vitro antimikrobiyal ve sitotoksik etki göstermiştir

Abstract

Mentha pulegium tıbbi açıdan önemli ve iyi bilinen bir bitkidir ve tıbbi, beslenme ve baharat gibi çeşitli amaçlar için kullanılır. Bu çalışmada M. pulegium ekstraktının antimikrobiyal, antioksidan ve sitotoksik özelliklerini görmek için analiz edildi. M. pulegium'un antimikrobiyal aktivitesi agar kuyucuk yöntemi kullanılarak test edildi. MIC, MBC ve antimikrobiyal aktivite Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25322), Bacillus megaterium (DSM32) ve Candida albicans (FMC17) mikroorganizmaları üzerinde test edildi. Pozitif kontrol olarak Klindamisin (2 µg) ve Amoksisilin (30 µg), negatif kontrol olarak Dimetilsülfoksit (DMSO) kullanıldı. 3-(4,5-dimetiltiyazol-2-il) -2,5-difenil tetrazolyum bromür (MTT) test yöntemi kullanılarak aseton, kloroform ve metanol gibi çözücülerden elde edilen farklı konsantrasyonlarda ekstraktların sitotoksik aktivitesi; ekstraktların farklı konsantrasyonlarına maruz kaldıktan sonra µg/ml sitotoksik aktivite ve hücre ölüm şekilleri; Aseton, kloroform ve metanol gibi çözücülerden 3-(4,5-dimetiltiyazol-2-il) -2,5-difenil tetrazolyum bromür (MTT) test yöntemi kullanılarak aseton, kloroform ve metanol insan meme kanseri (MDA-MB-231), insan pankreas kanseri (PANC1), insan yumurtalık kanseri (OVCAR3) ve insan akciğer kanseri (A549) hücre hatları buna göre belirlendi. Sonuç olarak M. pulegium ekstraktının güçlü bir antimikrobiyal aktivite spektrumuna ve sitotoksik etkiye sahip olduğu belirlenmiştir.

Keywords

Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro.

Abstract

Mentha pulegium is a medicinally important and well-known plant and used for various purposes such as medicinal, nutritional and spice. We are analyzed to observe the antimicrobial, antioxidant and cytotoxic properties of M. pulegium extracts in this study. The antimicrobial activity of M. pulegium was tested using the agar well method. MIC, MBC and antimicrobial activity were tested on Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25322), Bacillus megaterium (DSM32) and Candida albicans (FMC17) microorganisms. Clindamycin (2 µg) and Amoxicillin (30 µg) were used as positive control and Dimethylsulfoxide (DMSO) as negative control. Cytotoxic activity of extracts at different concentrations obtained from solvents such as acetone, chloroform and methanol using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test method; µg/ml cytotoxic activity and cell death patterns after exposure to different concentrations of the extracts on human breast cancer (MDA-MB-231), human pancreatic cancer (PANC1), human ovarian cancer (OVCAR3) and human lung cancer (A549) cell lines determined accordingly. As a result, it was determined that M. pulegium extract has a strong antimicrobial activity spectrum and cytotoxic effect.

Keywords

Supporting Institution

TÜBİTAK

Project Number

1919B012001186

Thanks

Thanks to Prof. Dr. Mustafa Kaplan, Fırat University Faculty of Medicine Department of Parasitology, for contributing to the laboratory environment in the realization of this study.

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Semih Dalkılıç ^*
0000-0002-6892-247X
Türkiye

Lütfiye Kadıoğlu Dalkılıç
0000-0002-6791-3811
Türkiye

Songül Fidan
0000-0003-1876-858X
Türkiye

Mustafa Timurkaan
0000-0003-1950-0489
Türkiye

Lütfü Uygur
0000-0002-5113-2703
Türkiye

Gökhan Akay
0000-0003-3892-2572
Türkiye

İsmail Korkmaz
0000-0003-4631-7786
Türkiye

Early Pub Date

June 14, 2023

Publication Date

December 31, 2023

Submission Date

October 20, 2022

Acceptance Date

February 27, 2023

Published in Issue

Year 2023 Volume: 26 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1192081

IZ

https://izlik.org/JA27GR67UZ

Cite

RIS / Bibtex

APA

Dalkılıç, S., Kadıoğlu Dalkılıç, L., Fidan, S., Timurkaan, M., Uygur, L., Akay, G., & Korkmaz, İ. (2023). Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(6), 1291-1304. https://doi.org/10.18016/ksutarimdoga.vi.1192081

AMA

1.Dalkılıç S, Kadıoğlu Dalkılıç L, Fidan S, et al. Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro. KSU J. Agric Nat. 2023;26(6):1291-1304. doi:10.18016/ksutarimdoga.vi.1192081

Chicago

Dalkılıç, Semih, Lütfiye Kadıoğlu Dalkılıç, Songül Fidan, et al. 2023. “Mentha Pulegium Extracts Showed Strong Antimicrobial and Cytotoxic Effects in Vitro”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 26 (6): 1291-1304. https://doi.org/10.18016/ksutarimdoga.vi.1192081.

EndNote

Dalkılıç S, Kadıoğlu Dalkılıç L, Fidan S, Timurkaan M, Uygur L, Akay G, Korkmaz İ (December 1, 2023) Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 26 6 1291–1304.

IEEE

[1]S. Dalkılıç et al., “Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro”., KSU J. Agric Nat., vol. 26, no. 6, pp. 1291–1304, Dec. 2023, doi: 10.18016/ksutarimdoga.vi.1192081.

ISNAD

Dalkılıç, Semih - Kadıoğlu Dalkılıç, Lütfiye - Fidan, Songül - Timurkaan, Mustafa - Uygur, Lütfü - Akay, Gökhan - Korkmaz, İsmail. “Mentha Pulegium Extracts Showed Strong Antimicrobial and Cytotoxic Effects in Vitro”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 26/6 (December 1, 2023): 1291-1304. https://doi.org/10.18016/ksutarimdoga.vi.1192081.

JAMA

1.Dalkılıç S, Kadıoğlu Dalkılıç L, Fidan S, Timurkaan M, Uygur L, Akay G, Korkmaz İ. Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro. KSU J. Agric Nat. 2023;26:1291–1304.

MLA

Dalkılıç, Semih, et al. “Mentha Pulegium Extracts Showed Strong Antimicrobial and Cytotoxic Effects in Vitro”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 26, no. 6, Dec. 2023, pp. 1291-04, doi:10.18016/ksutarimdoga.vi.1192081.

Vancouver

1.Semih Dalkılıç, Lütfiye Kadıoğlu Dalkılıç, Songül Fidan, Mustafa Timurkaan, Lütfü Uygur, Gökhan Akay, İsmail Korkmaz. Mentha pulegium extracts showed strong antimicrobial and cytotoxic effects in vitro. KSU J. Agric Nat. 2023 Dec. 1;26(6):1291-304. doi:10.18016/ksutarimdoga.vi.1192081

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