Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı

Bülent Başyiğit

doi:10.18016/ksutarimdoga.vi.1197761

TR EN

Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı

Abstract

Bu çalışmada yağı alınmış nar çekirdeklerin fenolik antioksidanların uygun şartlarda özütlenmesine odaklanılmıştır. Bu bağlamda özütleme adımında ultrases sistemi kullanılmış ve şartlar yanıt yüzey yöntemi ile optimize edilmiştir. Optimizasyon işleminde, özütleme süresinin (5-60 dk) ve ultrases cihazının genliğinin (%20-100) toplam fenolik madde miktarı üzerine etkisi araştırılmıştır. Maksimum toplam fenolik madde miktarı (TFM), özütleme süresinin 52 dk ve genliğin %88 olduğu noktada elde edilmiştir. Optimum koşullarda elde edilen özütlere ait özellikler klasik yöntemle (metanolik özütler) elde edilen özütlerle karşılaştırmalı olarak verilmiştir. Ultrases sistemi kullanılarak elde edilen özütlerin TFM (2.94 mg GAE g-1), toplam flavanoid madde miktarı (TFMM) (0.36 mg KE g-1) ve toplam hidrolize tanen madde miktarı (THTM) (22.07 mg TAE g-1) metanolik özütlerden (2.60 mg GAE g-1, 0.27 mg KE g-1, 16.73 mg TAE g-1) daha yüksek olduğu tespit edilmiştir. LC-ESI-MS/MS sonuçları yağsız nar çekirdeklerinin gallik asit ve ellajik asit açısından zengin olduğunu göstermiştir. Fenolik asitlerin baskınlığı FTIR spektroskopisi ile doğrulanmıştır. Üstün antioksidatif davranış optimum koşullarda hazırlanan özütlerde (DPPH: 105.26 µmol TEAC g-1, ABTS: 57.65 µmol TEAC g-1, FRAP: 13.03 µmol TEAC g-1, CUPRAC: 8.91 µmol TEAC g-1) tespit edilmiştir. Sonuçlar, meyve çekirdeklerden biyoaktif maddelerin özütlenmesinde ultrases sisteminin efektif bir uygulama olduğunu ortaya koymuştur.

Keywords

Use of Ultrasound System in Extraction of Phenolic Antioxidants from Oil-Free Pomegranate Seeds

Abstract

This study focused on the extraction of phenolic antioxidants from oil-free pomegranate seeds under suitable conditions. In this context, the ultrasound system was applied in the extraction step and the conditions were optimized by the response surface method. The effects of the extraction time (5-60 min) and the amplitude (20-100%) on total phenolic content were investigated. The optimum conditions were 52 min and 88% amplitude for providing maximum total phenolic content. The attributes of the extracts produced under optimum conditions were compared with the extracts obtained by the classical method (methanolic extracts). Total contents of phenolic (2.94 mg GAE g-1), flavonoid (0.36 mg CE g-1), and hydrolysable tannin (22.07 mg TAE g-1) in the extracts prepared using ultrasound assisted system were superior than those of methanolic extracts (2.60 mg GAE g-1, 0.27 mg CE g-1, 16.73 mg TAE g-1). LC-ESI-MS/MS results indicated that defatted pomegranate seeds were rich in gallic acid and ellagic acid. The predominance of phenolic acids was endorsed by FTIR spectroscopy. The extracts produced in the optimum conditions exhibited higher antioxidative behavior (DPPH: 105.26 µmol TEAC g-1, ABTS: 57.65 µmol TEAC g-1, FRAP: 13.03 µmol TEAC g-1, CUPRAC: 8.91 µmol TEAC g-1). The results indicated that ultrasound system created awareness in terms of the effective extraction of bioactive substances from fruit seeds.

Keywords

References

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Details

Primary Language

Turkish

Subjects

Food Properties

Journal Section

Research Article

Authors

Bülent Başyiğit ^*
0000-0002-6617-1836
Türkiye

Early Pub Date

June 14, 2023

Publication Date

December 31, 2023

Submission Date

November 1, 2022

Acceptance Date

June 1, 2023

Published in Issue

Year 2023 Volume: 26 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1197761

IZ

https://izlik.org/JA38UZ73AD

Cite

RIS / Bibtex

APA

Başyiğit, B. (2023). Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(6), 1346-1357. https://doi.org/10.18016/ksutarimdoga.vi.1197761

AMA

1.Başyiğit B. Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı. KSU J. Agric Nat. 2023;26(6):1346-1357. doi:10.18016/ksutarimdoga.vi.1197761

Chicago

Başyiğit, Bülent. 2023. “Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 26 (6): 1346-57. https://doi.org/10.18016/ksutarimdoga.vi.1197761.

EndNote

Başyiğit B (December 1, 2023) Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 26 6 1346–1357.

IEEE

[1]B. Başyiğit, “Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı”, KSU J. Agric Nat., vol. 26, no. 6, pp. 1346–1357, Dec. 2023, doi: 10.18016/ksutarimdoga.vi.1197761.

ISNAD

Başyiğit, Bülent. “Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 26/6 (December 1, 2023): 1346-1357. https://doi.org/10.18016/ksutarimdoga.vi.1197761.

JAMA

1.Başyiğit B. Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı. KSU J. Agric Nat. 2023;26:1346–1357.

MLA

Başyiğit, Bülent. “Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 26, no. 6, Dec. 2023, pp. 1346-57, doi:10.18016/ksutarimdoga.vi.1197761.

Vancouver

1.Bülent Başyiğit. Yağı Alınmış Nar Çekirdeklerinden Fenolik Antioksidanların Özütlenmesinde Ultrases Sisteminin Kullanımı. KSU J. Agric Nat. 2023 Dec. 1;26(6):1346-57. doi:10.18016/ksutarimdoga.vi.1197761

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