Farklı Yıllarda Toplanan Benli Şalba (Salvia pisidica Boiss. & Heldr. ex Bentham) Türünün Sitotoksik Etkisinin Araştırılması

Orhan Ünal; Serap Özkaya; Esra Aydemir; Mehmet Torun

doi:10.18016/ksutarimdoga.vi.1205655

EN TR

Investigation of the Cytotoxic Effect of Benli Şalba (Salvia pisidica Boiss. & Heldr. ex Bentham) Species Collected in Different Years

Abstract

Cancer is a systemic disease that occurs with the accumulation of many mutations in the genome. Due to the different treatment methods used in cancer treatments, it prevents the success of the treatments due to the high toxicity and drug resistance in the cells over time. Therefore, natural products and their synthetic derivatives have great potential for cancer treatment. Benli Şalba (Salvia pisidica Boiss.&Heldr. ex Bentham) has many biological activities, especially antibacterial and antioxidative effects. In this study, the cytotoxic effects of water extracts of plants collected in different years against breast cancer cells were investigated. In this study, 24, 48 and 72 hour incubations were made in MCF-7, MDA-MB-231, and MCF10-A cell lines. The water extracts of S.pisidica plant collected in 2017, 2018 and 2019 were applied to the cells at doses of 1-1000 µg/mL. At the end of the incubation period, cytotoxicity test was performed with the WST-8 cell viability determination kit. In the results of the experiment, it was calculated that the IC50 value of the extract of 2017 was 1.95 ng/mL in 48 hours of incubation, and the IC40 value of the extract of 2019 was 3.9 and 1.95 µg/mL in 48 hours of incubation in MCF-7 cells. In the more aggressive MDA cells, the IC50 value of the 2019 S.pisidica water extract in 48 hours of incubation is 7.8 µg/mL. When the results obtained were examined, it was found that the phenolic contents differed depending on the year and were low in the samples of 2017 and high in the samples of 2019.

Keywords

Farklı Yıllarda Toplanan Benli Şalba (Salvia pisidica Boiss. & Heldr. ex Bentham) Türünün Sitotoksik Etkisinin Araştırılması

Öz

Kanser, genomdaki birçok mutasyonun birikimi ile ortaya çıkan sistemik bir hastalıktır. Kanser tedavilerinde kullanılan farklı tedavi yöntemleri nedeniyle zamanla hücrelerde yüksek toksisite ve ilaç direncine neden olması sebebiyle tedavilerdeki başarıyı engellemektedir. Bu nedenle kanser tedavisi için doğal ürünler ve bunların sentetik türevleri büyük bir potansiyel taşımaktadır. Benli Şalba (Salvia pisidica Boiss.&Heldr. Ex Bentham) antibakteriyel ve antioksidatif etki başta olmak üzere birçok biyolojik aktiviteye sahiptir. Bu çalışmada farklı yıllarda toplanan bitkileren elde edilen su ekstraktlarının meme kanser hücrelerinde sergilediği sitotoksik etkiler incelenmiştir. Çalışmada 24, 48 ve 72 saatlik inkübasyonlar MCF-7, MDA-MB-231 ve MCF10-A hücre hatlarında yapılmıştır. 2017, 2018 ve 2019 yıllarında toplanan S.pisidica bitkisinin su ekstraktları 1-1000 ng/mL dozlarda hücrelere uygulanmıştır. WST-8 hücre canlılığı belirleme kiti ile sitotoksisite testi inkübasyon süreleri sonunda yapılmıştır. Çalışmalar sonucunda MCF-7 hücrelerinde 2017 yılına ait ekstraktın 48 saatlik inkübasyonda IC50 değeri 1.95 ng/mL, 2019 yılına ait ekstraktın 48 saatlik inkübasyonda IC40 değeri 3.9 ve 1.95 ng/mL olduğu hesaplanmıştır. Daha agresif olan MDA hücrelerinde 2019 yılına ait S.pisidica su ekstraktının 48 saatlik inkübasyondaki IC50 değeri ise 7.8 ng/mL’dir. Elde edilen sonuçlara bakıldığında fenolik içeriklerin yıllara bağlı olarak farklılık gösterdiği ve 2017 örneğinde düşük 2019 örneğinde ise yüksek değerlerde olduğu bulunmuştur.

Anahtar Kelimeler

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Details

Primary Language

Turkish

Subjects

Structural Biology

Journal Section

Research Article

Authors

Orhan Ünal ^*
0000-0001-9633-5726
Türkiye

Serap Özkaya
0000-0002-7071-4805
Türkiye

Esra Aydemir
0000-0002-5206-7333
Türkiye

Mehmet Torun
0000-0002-6287-2993
Türkiye

Early Pub Date

June 14, 2023

Publication Date

December 31, 2023

Submission Date

November 16, 2022

Acceptance Date

May 5, 2023

Published in Issue

Year 2023 Volume: 26 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1205655

IZ

https://izlik.org/JA78KL55UX

Cite

RIS / Bibtex

APA

Ünal, O., Özkaya, S., Aydemir, E., & Torun, M. (2023). Farklı Yıllarda Toplanan Benli Şalba (Salvia pisidica Boiss. & Heldr. ex Bentham) Türünün Sitotoksik Etkisinin Araştırılması. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 26(6), 1268-1276. https://doi.org/10.18016/ksutarimdoga.vi.1205655

Cited By

Investigation of volatile oil contents and some ecological characteristics of wild and cultivated Sideritis stricta Boiss. & Heldr.

Prospects in Pharmaceutical Sciences

https://doi.org/10.56782/pps.255