Quantification of Some Phenolic Compounds in Consolida thirkeana (Boiss.) Bornm. by HPLC and Validation of Method

Derya Çiçek Polat; Muhammed Mesud Hürkul

doi:10.18016/ksutarimdoga.vi.1210096

TR EN

Consolida thirkeana (Boiss.) Bornm.’daki Bazı Fenolik Bileşiklerin YPSK ile Miktarı Tayini ve Yöntemin Validasyonu

An Erratum to this article was published on December 31, 2024. http://dergipark.org.tr/en/pub/ksutarimdoga/article/1441809

Öz

Consolida türleri başta deri hastalıkları olmak üzere çeşitli hastalıkların tedavisinde geleneksel kullanıma sahiptir. Türkiye'de de bazı Consolida türlerinin geleneksel kullanımı mevcuttur. Fenolik bileşiklerin önemli farmakolojik etkileri bulunmaktadır, bu nedenle bitkilerde miktarlarının bilinmesi önemli bir yere sahiptir. Consolida thirkeana, Türkiye'de “boz mahmuz” olarak bilinen endemik bir türdür ve fenolik bileşikler açısından herhangi bir çalışmaya rastlanmamıştır. Bu nedenle C. thikeana için bazı fenolik bileşiklerin miktar tayini yapılmıştır. Bu çalışmada, C. thirkeana, YPSK kullanılarak kafeik asit, klorojenik asit, hiperosit ve rutin açısından kantitatif olarak analiz edilmiş ve yöntemin validasyonu (doğrusallık, kesinlik, doğruluk, geri kazanım, tespit limitleri ve ölçüm limitleri) gerçekleştirilmiştir. Toprak üstü kısımlarında, klorojenik asit (%0,098), kafeik asit (%0,107), rutin (%0,078) ve hiperosit (%0,134) tespit edilirken, toprak altı kısmı sadece rutin (%0,007) tespit edilmiştir. Bu çalışma sonucunda bu endemik tür bazı fenolik bileşikler açısından değerlendirilmiştir. Bu yöntem kullanılarak diğer Consolida türleri üzerinde fenolik bileşiklerin tayininin yapılabileceği düşünülmektedir.

Anahtar Kelimeler

Quantification of Some Phenolic Compounds in Consolida thirkeana (Boiss.) Bornm. by HPLC and Validation of Method

An Erratum to this article was published on December 31, 2024. http://dergipark.org.tr/en/pub/ksutarimdoga/article/1441809

Abstract

Consolida species have traditional uses in the treatment of various diseases, especially skin diseases. There is also traditional use of some Consolida species in Turkey. Phenolic compounds have significant pharmacological effects, therefore it is important to know their amount in plants. Consolida thirkeana is endemic to Turkey and known as “boz mahmuz” and no study had conducted in terms of phenolic compounds. Therefore, some phenolic amount, which has been done for the first time for C. thikeana, was analyzed. In this study, C. thirkeana was analyzed quantitatively for caffeic acid, chlorogenic acid, hyperosit, and rutin by using HPLC and the method was validated (linearity, precision, accuracy, recovery, limits of detection (LOD), and limits of quantification (LOQ)). While chlorogenic acid (0.098%), caffeic acid (0.107%), rutin (0.078%), and hyperoside (0.134%) were detected in the aerial part, only rutin (0.007%) was detected in the root. As a result of this study, this endemic species was evaluated in terms of some phenolic compounds. It is thought that phenolic compounds can be determined on other Consolida species with this method.

Keywords

References

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Details

Primary Language

English

Subjects

Plant Biochemistry

Journal Section

Research Article

Authors

Derya Çiçek Polat ^*
0000-0002-4331-6828
Türkiye

Muhammed Mesud Hürkul
0000-0002-9241-2496
Türkiye

Early Pub Date

October 13, 2023

Publication Date

February 28, 2024

Submission Date

November 25, 2022

Acceptance Date

June 15, 2023

Published in Issue

Year 2024 Volume: 27 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1210096

IZ

https://izlik.org/JA93ZF78LR

Cite

RIS / Bibtex

APA

Çiçek Polat, D., & Hürkul, M. M. (2024). Quantification of Some Phenolic Compounds in Consolida thirkeana (Boiss.) Bornm. by HPLC and Validation of Method. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(1), 68-74. https://doi.org/10.18016/ksutarimdoga.vi.1210096