Naftalin Asetik Asit, 6-Benzilaminopürin ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma

Aykut Topdemir; Tuba Okutan; Görkem Kırmızıkaya; Prof. Dr. Ökkeş Yılmaz

doi:10.18016/ksutarimdoga.vi.1281016

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Naftalin Asetik Asit, 6-Benzilaminopürin ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma

Abstract

Bu çalışmada oksin türevi olan naftalin asetik asit (NAA), sitokinin türevi 6-Benzilaminopürin (BAP) ile indol-3-butirik asit (IBA)’in farklı konsantrasyonları ve kombinasyonları kivi bitkisinin (Actinidia deliciosa) kotiledon eksplantlarına uygulandı. Kallus hücrelerinin fenolik bileşik, antioksidan aktivite, yağ asidi bileşimi ve lipofilik molekül içerikleri incelendi. Kültür ortamında yetiştirilen Actinidia deliciosa sürgünleri 5 santimetreye ulaşınca kotiledonları eksplant kaynağı olarak kullanıldı. Farklı doz ve kombinasyonlarda bitki büyüme düzenleyicileri (BBD) içeren kültür ortamlarında kallus indüksiyonu sağlandı. Kalluslar ile yapılan analiz sonuçlarına göre total fenolik içeriği 0.5 mg L-1 NAA + 1 mg L-1 BAP + 0.5 mg L-1 IBA kombinasyonu içeren F grubunda kontrol grubuna göre azaldığı halde (p<0.05), diğer gruplarda belirgin düzeyde yüksek bulundu (p<0.001). DPPH ve ABTS antioksidan kapasite değerleri NAA ve BAP kombinasyonu verilen gruplarda yüksek olduğu halde, NAA+BAP+IBA kombinasyonun bulunduğu gruplarda düşük olduğu belirlendi. Antioksidan etkili lipofilik moleküllerden α-tokoferol düzeyi farklı konsantrasyon uygulanan NAA ve BAP gruplarında yüksek (p<0.001), farklı konsantrasyon kullanılan NAA ile BAP ve NAA+BAP+IBA kombinasyon gruplarında ise azaldığı saptandı (p<0.001). Ergosterol, stigmasterol ve betasitosterol gibi fitosterol düzeylerinin bitki büyüme düzenleyilecilerinin (BBD) uygulandığı kombinasyon gruplarında farklı oranlarda azaldığı gözlendi. Yağ asidi kombinasyonu içinde kontrol grubuna göre BBD gruplarının çoğunda palmitik asit oranı arttığı halde (p<0.01), bazı BBD gruplarında istatistik farklılık bulunmadı. BBD’nin farklı kombinasyonlarının verildiği grupların yağ asidi bileşimi içinde palmitoleik, stearik ve linoleik asit oranlarında azalma gözlenirken, linolenik asit oranlarında yükselme belirlendi (p<0.05, p<0.01, p<0.001). Sonuç olarak kivi bitkisinin kallus oluşumu üzerinde farklı oranlarda kullanılan oksin ve sitokinin türevlerinin kallus dokusunun biyokimyasal metabolitleri üzerinde farklı etkilere sahip olduğu belirlendi.

Keywords

A Biochemical Study on the Development of Actinidia deliciosa Callus by Combinations of Naphthalene Acetic Acid, 6-Benzylaminopurine and Indole-3-Butyric Acid

Abstract

In this study, different concentrations and combinations of auxin derivative naphthalene acetic acid (NAA), cytokinin derivative 6-Benzylaminopurine (BAP) and indole-3-butyric acid (IBA) were applied to cotyledon explants of kiwifruit (Actinidia deliciosa). Phenolic compound, antioxidant activity, fatty acid composition and lipophilic molecule contents of callus cells were investigated. When the shoots of Actinidia deliciosa grown in a culture medium reached 5 cm, their cotyledons were used as explant source. Callus induction was achieved in culture media containing plant growth regulators (BBD) in different doses and combinations. According to the results of the analysis made with calli, the total phenolic content of the F group containing 0.5 mg L-1 NAA + 1 mg L-1 BAP + 0.5 mg L-1 IBA combination was decreased compared to the control group (p<0.05), but it was significantly higher in the other groups. (p<0.001). Although DPPH and ABTS antioxidant capacity values were higher in the groups given NAA and BAP combination, it was determined that they were lower in the groups with NAA+BAP+IBA combination. The level of α-tocopherol, an antioxidant- effective lipophilic molecule, was higher in NAA and BAP groups applied at different concentrations (p<0.001), but decreased in NAA and BAP and NAA+BAP+IBA combination groups used in different concentrations (p<0.001). It was observed that phytosterol levels such as ergosterol, stigmasterol and betasitosterol decreased at different rates in the combination groups in which plant growth regulators (BBD) were applied. Although the palmitic acid ratio increased in most of the BBD groups compared to the control group in the fatty acid combination (p<0.01), no statistical difference was found in some BBD groups. While a decrease was observed in palmitoleic, stearic and linoleic acid ratios in the fatty acid composition of the groups in which different combinations of BBD were given, an increase was observed in linolenic acid ratios (p<0.05, p<0.01, p<0.001). As a result, it was determined that the auxin and cytokinin derivatives used at different rates on the callus formation of the kiwi plant had different effects on the biochemical metabolites of the callus tissue.

Keywords

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Details

Primary Language

Turkish

Subjects

Structural Biology

Journal Section

Research Article

Authors

Aykut Topdemir
0000-0002-9112-4767
Türkiye

Tuba Okutan ^*
0000-0001-8745-0343
Türkiye

Görkem Kırmızıkaya
0000-0001-8516-4933
Türkiye

Prof. Dr. Ökkeş Yılmaz
0000-0002-8276-4498
Türkiye

Early Pub Date

January 21, 2024

Publication Date

April 1, 2024

Submission Date

April 12, 2023

Acceptance Date

August 25, 2023

Published in Issue

Year 2024 Volume: 27 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1281016

IZ

https://izlik.org/JA85DL94AZ

Cite

RIS / Bibtex

APA

Topdemir, A., Okutan, T., Kırmızıkaya, G., & Yılmaz, P. D. Ö. (2024). Naftalin Asetik Asit, 6-Benzilaminopürin ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(2), 249-260. https://doi.org/10.18016/ksutarimdoga.vi.1281016

AMA

1.Topdemir A, Okutan T, Kırmızıkaya G, Yılmaz PD Ö. Naftalin Asetik Asit, 6-Benzilaminopürin ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma. KSU J. Agric Nat. 2024;27(2):249-260. doi:10.18016/ksutarimdoga.vi.1281016

Chicago

Topdemir, Aykut, Tuba Okutan, Görkem Kırmızıkaya, and Prof. Dr. Ökkeş Yılmaz. 2024. “Naftalin Asetik Asit, 6-Benzilaminopürin Ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia Deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 27 (2): 249-60. https://doi.org/10.18016/ksutarimdoga.vi.1281016.

EndNote

Topdemir A, Okutan T, Kırmızıkaya G, Yılmaz PD Ö (April 1, 2024) Naftalin Asetik Asit, 6-Benzilaminopürin ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 27 2 249–260.

IEEE

[1]A. Topdemir, T. Okutan, G. Kırmızıkaya, and P. D. Ö. Yılmaz, “Naftalin Asetik Asit, 6-Benzilaminopürin ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma”, KSU J. Agric Nat., vol. 27, no. 2, pp. 249–260, Apr. 2024, doi: 10.18016/ksutarimdoga.vi.1281016.

ISNAD

Topdemir, Aykut - Okutan, Tuba - Kırmızıkaya, Görkem - Yılmaz, Prof. Dr. Ökkeş. “Naftalin Asetik Asit, 6-Benzilaminopürin Ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia Deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 27/2 (April 1, 2024): 249-260. https://doi.org/10.18016/ksutarimdoga.vi.1281016.

JAMA

1.Topdemir A, Okutan T, Kırmızıkaya G, Yılmaz PD Ö. Naftalin Asetik Asit, 6-Benzilaminopürin ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma. KSU J. Agric Nat. 2024;27:249–260.

MLA

Topdemir, Aykut, et al. “Naftalin Asetik Asit, 6-Benzilaminopürin Ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia Deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 27, no. 2, Apr. 2024, pp. 249-60, doi:10.18016/ksutarimdoga.vi.1281016.

Vancouver

1.Aykut Topdemir, Tuba Okutan, Görkem Kırmızıkaya, Prof. Dr. Ökkeş Yılmaz. Naftalin Asetik Asit, 6-Benzilaminopürin ve İndol-3-Bütirik Asit Kombinasyonlarının Actinidia deliciosa Kallus Gelişimi Üzerine Biyokimyasal Bir Araştırma. KSU J. Agric Nat. 2024 Apr. 1;27(2):249-60. doi:10.18016/ksutarimdoga.vi.1281016

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