Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts: Comparison with MCF-7 and MCF-12A Cells

Fadime Beyazyüz; Emine Arslan; Gozde Koygun

doi:10.18016/ksutarimdoga.vi.1292478

EN TR

Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts: Comparison with MCF-7 and MCF-12A Cells

Abstract

Jerusalem artichoke (Helianthus tuberosus L.) is an important medicinal plant due to its composition of several bioactive substances, high antioxidant activity, rich inulin content and produces functional food ingredients. Complementary alternative medicine therapies become more popular for cancer treatments because of higher efficiency, lower cost and minimum side effects. This study reported anti-cancer efficiency of both shell and tuber extract of Jerusalem artichoke (JA) on different breast cell lines with the purpose of discovering new alternative medicine therapies. Cytotoxic effects of JA were evaluated by XTT method by using different concentrations on different incubation times (24h, 48h and 72h). Invasion, adhesion and apoptotic studies were performed with IC50 values for 48h and 72h. JA extracts do not show cytotoxic activity on healthy human breast cell, while they induce tumoral cell death with dose and time-dependent manner. The most effective doses of tuber and shell extracts were 108.8 µM and 134.2 µM at 48h and 20 µM ve 5 µM at 72h, respectively. It demonstrated robust anti-metastatic activity on MCF-7 cell lines because it inhibited adhesion, and invasion, significantly. TMRE and AnnexinV/7AAD staining were used for determine to mitochondrial membrane potential and nuclear morphology, respectively The results were found consistent with each other. Overall, this paper which first in literature demonstrates the anti-cancer efficacy of the JA both tuber and shell extract which can be proposed as a potent candidate for the treatment of breast cancer.

Keywords

Yer Elması (Helianthus tuberosus L.) Ekstraktlarının Sitotoksik, Antimetastatik ve Apoptotik Aktivitelerinin Araştırılması: MCF-7 ve MCF-12A Hücrelerinin Karşılaştırılması

Abstract

Tamamlayıcı alternatif tıp tedavileri, daha yüksek verim, daha düşük maliyet ve yan etkilerinin minimum olması nedeniyle kanser tedavileri için daha popüler hale gelmektedir. Bu bağlamda çeşitli biyoaktif maddelerden oluşması, yüksek antioksidan aktivitesi, zengin inülin içeriği ve fonksiyonel gıda bileşenleri üretmesi nedeniyle yer elması (Helianthus tuberosus L.) önemli bir tıbbi bitkidir. Bu çalışma, yeni alternatif tıp tedavilerini keşfetmek amacıyla, yer elmasının hem kabuk hem de yumru özütünün farklı meme hücre hatları üzerinde kansere karşı etkinliğini araştırmak için yapılmıştır. Yer elmasının sitotoksik etkileri, farklı inkübasyon sürelerinde (24s,48s,72s) farklı konsantrasyonlar kullanılarak XTT yöntemi ile değerlendirilmiştir. İnvazyon, adezyon ve apoptotik çalışmalar 48 saat ve 72 saat uygulamalardan elde edilen IC50 değerlerine göre yürütülmüştür. Yer elması özütleri sağlıklı insan meme hücresi üzerinde sitotoksik aktivite göstermezken, doz ve zamana bağlı olarak tümöral hücre ölümünü indüklemiştir. Yumru ve kabuk özlerinin en etkili dozları 48 saatte sırasıyla 108.8 µM ve 134.2 µM ve 72 saatte 20 µM ve 5 µM olarak gözlenmiştir. Yer elması özleri MCF-7 hücre hatları üzerinde adezyon ve invazyonu önemli ölçüde engellemesi nedeniyle güçlü bir anti-metastatik aktivite göstermiştir. Mitokondriyal membran potansiyelini ve nükleer morfolojiyi belirlemek için sırasıyla TMRE ve AnnexinV/ 7AAD boyaması kullanıldı. Sonuçlar birbiriyle uyumlu bulundu. Literatürde bir ilk olan bu çalışma, yer elmasının hem yumru hem de kabuk özütünün anti-kanser etkinliğini ortaya koyarak meme kanseri tedavisi için önerilebilir.

Keywords

Supporting Institution

BAP

Project Number

17201144

Thanks

This research was supported by Selçuk University Scientific Research Project (BAP) (No. 17201144). This study was presented as an oral presentation 1st International Plant Biology Congress in 2018(10-12May) and published as an abstract in the congrees book. F. B and E.A. conceived the original idea and build E.A. supervised the project. The experiments were performed by F.B and G.K. I am grateful to Elif GÜLBAHÇE MUTLU for the plant collection and extraction steps of the experiment, and I would like to thank Mert SUDAĞIDAN for helping us with the devices for the experiments.

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Fadime Beyazyüz ^*
0000-0003-3629-0559
Türkiye

Emine Arslan
0000-0002-0782-506X
Türkiye

Gozde Koygun
0000-0003-1571-2986
Türkiye

Early Pub Date

January 21, 2024

Publication Date

April 1, 2024

Submission Date

May 5, 2023

Acceptance Date

September 28, 2023

Published in Issue

Year 2024 Volume: 27 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1292478

IZ

https://izlik.org/JA85RG55WT

Cite

RIS / Bibtex

APA

Beyazyüz, F., Arslan, E., & Koygun, G. (2024). Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts: Comparison with MCF-7 and MCF-12A Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(2), 293-303. https://doi.org/10.18016/ksutarimdoga.vi.1292478

AMA

1.Beyazyüz F, Arslan E, Koygun G. Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts: Comparison with MCF-7 and MCF-12A Cells. KSU J. Agric Nat. 2024;27(2):293-303. doi:10.18016/ksutarimdoga.vi.1292478

Chicago

Beyazyüz, Fadime, Emine Arslan, and Gozde Koygun. 2024. “Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus Tuberosus L.) Extracts: Comparison With MCF-7 and MCF-12A Cells”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 27 (2): 293-303. https://doi.org/10.18016/ksutarimdoga.vi.1292478.

EndNote

Beyazyüz F, Arslan E, Koygun G (April 1, 2024) Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts: Comparison with MCF-7 and MCF-12A Cells. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 27 2 293–303.

IEEE

[1]F. Beyazyüz, E. Arslan, and G. Koygun, “Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts: Comparison with MCF-7 and MCF-12A Cells”, KSU J. Agric Nat., vol. 27, no. 2, pp. 293–303, Apr. 2024, doi: 10.18016/ksutarimdoga.vi.1292478.

ISNAD

Beyazyüz, Fadime - Arslan, Emine - Koygun, Gozde. “Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus Tuberosus L.) Extracts: Comparison With MCF-7 and MCF-12A Cells”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 27/2 (April 1, 2024): 293-303. https://doi.org/10.18016/ksutarimdoga.vi.1292478.

JAMA

1.Beyazyüz F, Arslan E, Koygun G. Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts: Comparison with MCF-7 and MCF-12A Cells. KSU J. Agric Nat. 2024;27:293–303.

MLA

Beyazyüz, Fadime, et al. “Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus Tuberosus L.) Extracts: Comparison With MCF-7 and MCF-12A Cells”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 27, no. 2, Apr. 2024, pp. 293-0, doi:10.18016/ksutarimdoga.vi.1292478.

Vancouver

1.Fadime Beyazyüz, Emine Arslan, Gozde Koygun. Investigation of Cytotoxic, Antimetastatic and Apoptotic Activities of Jerusalem Artichoke (Helianthus tuberosus L.) Extracts: Comparison with MCF-7 and MCF-12A Cells. KSU J. Agric Nat. 2024 Apr. 1;27(2):293-30. doi:10.18016/ksutarimdoga.vi.1292478

Cited By

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Pharmaceuticals

https://doi.org/10.3390/ph17121672

Phytochemical Composition and Mechanistic Pharmacology of Jerusalem Artichoke (Helianthus tuberosus L.): Implications for Functional and Therapeutic Applications

Current Issues in Molecular Biology

https://doi.org/10.3390/cimb48020180