Assessment of Inhibitory Ability Against Medicinally Important Enzymes with Invitro and In Silico Studies: Phenolic Content of Endemic Centaurea cadmea subsp. pontica

Dursun Kısa; Ahmet Çelik; Rizvan İmamoğlu

doi:10.18016/ksutarimdoga.vi.1294720

TR EN

Tıbbi Açıdan Önemli Enzimlere Karşı İnhibisyon Yeteneğinin Invitro ve Insilico Çalışmalarla Değerlendirilmesi: Endemik Centaurea cadmea subsp. pontica Bitkisinin Fenolik İçeriği

Öz

Centaurea türleri geleneksel şifalı bitki olarak büyük bir potansiyele sahiptir. Küre Dağı'nın kayalık yamaç yarıklarından toplanan C. cadmea subsp. pontica Türkiye florasına endemiktir. Bu çalışmada, bitkinin farmakolojik önemini ortaya koymak için çeşitli tıbbi enzimleri inhibe etme pontansiyeli, moleküler yerleştirme çalışmaları ile desteklenerek araştırılmıştır. Çalışılan enzimler için yarı maksimum inhibitör konsantrasyon (IC50) sonuçları 0.50-86.97 µg mLˉˡ arasında belirlendi. Bitki ekstraktı diyabet ve kolesterol ile bağlantılı olan HMG_CoA R, α-amilaz ve α -glukosidaz enzimlerine karşı etki gösterdi. C. cadmea subsp. pontica özütünde dokuz fenolik bileşik tanımlandı ve en fazla bulunan fenolik bileşiklerin enzimlerle etkileşimleri moleküler yerleştirme çalışmaları ile incelenmiştir. Sonuç olarak, mevcut çalışmadan elde edilen bulgular, bu endemik bitkinin sağlıklı yaşam kalitesinin geliştirilmesine yönelik yeni bileşenlerin formüle edilmesi için etkili bir bitki olarak yararlı olabileceğini göstermektedir.

Anahtar Kelimeler

Assessment of Inhibitory Ability Against Medicinally Important Enzymes with Invitro and In Silico Studies: Phenolic Content of Endemic Centaurea cadmea subsp. pontica

Abstract

Centaurea species has great potential as a traditional medicinal herb and C. cadmea subsp. pontica collected from rocky slope crevices of Küre Mountain is endemic to the flora of Türkiye. In the present work, to reveal the plant's pharmacological importance, its potency to inhibit various medicinal enzymes was investigated, supported by molecular docking studies. The half-maximal inhibitory concentration (IC50) results for studied enzymes were quantified between 0.50-86.97 µg mLˉˡ, and the extract was efficient against HMG_CoA R, α-glucosidase, and α-amylase enzymes linked to diabetes and cholesterol. Nine phenolic compounds were identified in the C. cadmea subsp. pontica extract and the interactions of the most abundant phenolic compounds with the enzymes were examined with molecular docking studies. In conclusion, findings amassed from the present study inclined to support the opinion that C. cadmea subsp. pontica may be beneficial as an effective herb for formulating novel health-promoting ingredients.

Keywords

Supporting Institution

Bartın University

Project Number

2021-FEN-A-004

Thanks

The present study was financially supported with a research grant coded 2021-FEN-A-004 by the Scientific Research Projects Unit of Bartın University, Türkiye. We are very grateful to Prof. Dr. Zafer Kaya, who identified the plant and passed away from this world.

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Dursun Kısa ^*
0000-0002-7681-2385
Türkiye

Ahmet Çelik
0000-0001-9802-4868
Türkiye

Rizvan İmamoğlu
0000-0002-6306-4760
Türkiye

Early Pub Date

October 13, 2023

Publication Date

February 28, 2024

Submission Date

May 9, 2023

Acceptance Date

July 28, 2023

Published in Issue

Year 2024 Volume: 27 Number: 1

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1294720

IZ

https://izlik.org/JA22DH37FT

Cite

RIS / Bibtex

APA

Kısa, D., Çelik, A., & İmamoğlu, R. (2024). Assessment of Inhibitory Ability Against Medicinally Important Enzymes with Invitro and In Silico Studies: Phenolic Content of Endemic Centaurea cadmea subsp. pontica. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(1), 14-25. https://doi.org/10.18016/ksutarimdoga.vi.1294720

Cited By

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Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1496499