Green Synthesis and Antibiofilm Activity of Silver Nanoparticles by Camellia sinensis L. (White Tea Leaf)

İlke Karakaş; Nurcihan Hacıoğlu; Bahri Emirhan Özdemir

doi:10.18016/ksutarimdoga.vi.1297130

TR EN

Camellia sinensis L. (Beyaz Çay Yaprağı) ile Gümüş Nanopartiküllerin Yeşil Sentezi ve Antibiyofilm Aktivitesi

Öz

Green Synthesis and Antibiofilm Activity of Silver Nanoparticles by Camellia sinensis L. (White Tea Leaf) Biosynthesis of nanoparticles refers to the production or synthesis of nanoparticles using organisms, such as bacteria, fungi, plants or their byproducts. This approach offers several advantages over conventional chemical methods, including eco-friendliness, cost-effectiveness, and potential for large-scale production. The silver nanoparticles (AgNPs) synthesized using aqueous Camellia sinensis L. (white tea leaf) extracts as reducing and stabilizing agent were reported and evaluated for antibiofilm activity against test microorganisms in the study. The synthesized AgNPs were observed and characterized using Uv-Vis spectroscopic analysis, scanning electron and transmission electron microscopy energy-dispersive spectra and Fourier transform infrared spectroscopy. The synthesized AgNP were also screened for antibiofilm activity against Acinetobacter baumanii ATCC 19606 NRRLB 3704, Pseudomonas aeruginosa ATCC 27853 (Gram –ve), and Bacillus subtilis ATCC 6633, Staphylococcus haemolyticus ATCC 43252 (Gram +ve), and Candida albicans ATCC 10231. It was observed that AgNPs may be a good alternative therapeutic approach in future. Our results show that the synthesized AgNPs has the potential to be used for antibiofilm materials and different biomedical applications. Camellia sinensis L. (Beyaz Çay Yaprağı) ile Gümüş Nanopartiküllerin Yeşil Sentezi ve Antibiyofilm Aktivitesi Nanopartiküllerin biyosentezi bakteriler, mantarlar, bitkiler veya yan ürünleri gibi organizmaları kullanarak nanopartiküllerin üretimini veay sentezini ifade eder. Bu yaklaşım, çevre dostu, maliyet etkinliği ve büyük ölçekli üretim potansiyeli dahil olmak üzere geleneksel kimyasal yöntemlere göre çeşitli avantajlar sunar. Indirgeyici ve stabilize edici ajan olarak Camellia sinensis L. (beyaz çay yaprağı) ekstraktları kullanılarak sentezlenen gümüş nanopartiküller (AgNP’ler) rapor edilmiş ve çalışmada test mikroorganizmalarına karşı antibiyofilm aktivitesi açısından değerlendirilmiştir. Sentezlenen AgNP’ler Uv-Vis spektroskopik analizi, taramalı electron mikroskobu, enerji dağıtıcı spektrumları ve Fourier dönüşümü kızılötesi spektroskopisi kullanılarak gözlemlendi ve karakterize edildi. Sentezlenen AgNP’nin Acinetobacter baumanii ATCC 19606 NRRLB 3704, Pseudomonas aeruginosa ATCC 27853 (Gram –), Bacillus subtilis ATCC 6633, Staphylococcus haemolyticus ATCC 43252 (Gram +), ve Candida albicans ATCC 10231’a karşı antibiyofilm aktivitesi de taranmıştır. AgNP’lerin gelecekte iyi bir alternative tedavi yaklaşımı olabileceği gözlenmiştir. Sonuçlarımız, sentezlenen AgNP'lerin antibiyofilm materyalleri ve farklı biyomedikal uygulamalar için kullanılma potansiyeline sahip olduğunu göstermektedir.Nanopartiküllerin biyosentezi bakteriler, mantarlar, bitkiler veya yan ürünleri gibi organizmaları kullanarak nanopartiküllerin üretimini veya sentezini ifade eder. Bu yaklaşım, çevre dostu, maliyet etkinliği ve büyük ölçekli üretim potansiyeli dahil olmak üzere geleneksel kimyasal yöntemlere göre çeşitli avantajlar sunar. İndirgeyici ve stabilize edici ajan olarak Camellia sinensis L. (beyaz çay yaprağı) ekstraktları kullanılarak sentezlenen gümüş nanopartiküller (AgNP’ler) rapor edilmiş ve çalışmada test mikroorganizmalarına (Acinetobacter baumanii ATCC 19606 NRRLB 3704, Pseudomonas aeruginosa ATCC 27853 (Gram –), Bacillus subtilis ATCC 6633, Staphylococcus haemolyticus ATCC 43252 (Gram +), ve Candida albicans ATCC 10231) karşı antibiyofilm aktivitesi açısından değerlendirilmiştir. Sentezlenen AgNP’ler Uv-Vis spektroskopik analizi, taramalı elektron mikroskobu, enerji dağıtıcı spektrumları ve Fourier dönüşümü kızılötesi spektroskopisi kullanılarak gözlemlendi ve karakterize edildi. Sentezlenen AgNP’nin test mikroorganizmalarına karşı antibiyofilm aktivitesi de taranmıştır. Sonuçlarımız, sentezlenen AgNP'lerin antibiyofilm materyalleri ve farklı biyomedikal uygulamalar için kullanılma potansiyeline sahip olduğunu göstermektedir.

Anahtar Kelimeler

Green Synthesis and Antibiofilm Activity of Silver Nanoparticles by Camellia sinensis L. (White Tea Leaf)

Abstract

Biosynthesis of nanoparticles refers to the production or synthesis of nanoparticles using organisms, such as bacteria, fungi, plants or their byproducts. This approach offers several advantages over conventional chemical methods, including eco-friendliness, cost-effectiveness, and potential for large-scale production. The silver nanoparticles (AgNPs) synthesized using aqueous Camellia sinensis L. (white tea leaf) extracts as reducing and stabilizing agents were reported and evaluated for antibiofilm activity against test microorganisms (Acinetobacter baumanii ATCC 19606 NRRLB 3704, Pseudomonas aeruginosa ATCC 27853 (Gram –), and Bacillus subtilis ATCC 6633, Staphylococcus haemolyticus ATCC 43252 (Gram +), and Candida albicans ATCC 10231) in the study. The synthesized AgNPs were observed and characterized using Uv-Vis spectroscopic analysis, scanning electron and transmission electron microscopy energy-dispersive spectra and Fourier transform infrared spectroscopy. The synthesized AgNP was also screened for antibiofilm activity against test microorganisms. Our results show that the synthesized AgNPs have the potential to be used for antibiofilm materials and different biomedical applications.

Keywords

Supporting Institution

Tübitak

Project Number

2209-A

Thanks

This research is a part of Mr. Bahri Emirhan ÖZDEMİR’s TÜBİTAK-2209 A Project which is supported with the frame of Undergraduate Students Grant in Biological Science.

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

İlke Karakaş ^*
0000-0001-6596-0879
Türkiye

Nurcihan Hacıoğlu
0000-0002-5812-9398
Türkiye

Bahri Emirhan Özdemir
0000-0002-5870-1171
Türkiye

Early Pub Date

January 21, 2024

Publication Date

April 1, 2024

Submission Date

May 15, 2023

Acceptance Date

October 12, 2023

Published in Issue

Year 2024 Volume: 27 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1297130

IZ

https://izlik.org/JA23MC33SF

Cite

RIS / Bibtex

APA

Karakaş, İ., Hacıoğlu, N., & Özdemir, B. E. (2024). Green Synthesis and Antibiofilm Activity of Silver Nanoparticles by Camellia sinensis L. (White Tea Leaf). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(2), 285-292. https://doi.org/10.18016/ksutarimdoga.vi.1297130

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