Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster

Selda Öz; Zeynep Nur Sarıkaya; Özüm Larçın; Rabia Sarıkaya

doi:10.18016/ksutarimdoga.vi.1310729

TR EN

Fluoksetin Hidroklorür’ün Genotoksik Etkisinin Drosophila melanogaster’de Araştırılması

Abstract

Bu çalışmada depresyon tedavisinde yaygın olarak kullanılan bir antidepresan olan fluoksetin hidroklorürün (FLX-HCl) potansiyel genotoksik etkisinin Somatik Mutasyon ve Rekombinasyon Testi (SMART) kullanılarak belirlenmesi amaçlanmıştır. Deney grubunda çoklu kanat kılı (mwh) ve flare (flr3) mutasyonları için transheterozigot olan üçüncü dönem Drosophila melanogaster larvaları, farklı konsantrasyonlarda FLX-HCl (0.1, 0.5, 1 ve 2 mg/mL) içeren ortamda kronik olarak beslenmiştir. Distile su, 0.1 mM etil metan sülfonat (EMS) ve % 2 dimetil sülfoksit (DMSO) sırasıyla negatif, pozitif ve çözücü kontrol gruplarında kullanılmıştır. Deney ve kontrol gruplarında larvalar gelişimini tamamladığında hayatta kalan birey sayıları belirlenerek yaşama yüzdeleri hesaplanmıştır. Tüm uygulama gruplarında hem normal hem de serrat kanat fenotipine sahip 40 bireyin kanatları mikroskop altında incelenmiş ve kanatlardaki mutant klonlar sayılarak genetik değişimler değerlendirilmiştir. Elde edilen veriler, FLX-HCl'nin 1 ve 2 mg/mL konsantrasyonlarının D. melanogaster bireyleri üzerinde toksik etkiye neden olduğunu göstermiştir. Ayrıca FLX-HCl, D. melanogaster bireylerinde toplam mutasyon değerlendirmesi ve klon induksiyon frekansı bakımından 0.1 mg/mL konsantrasyonunda negatif, 0.5 mg/mL konsantrasyonunda önemsiz, 1 ve 2 mg/mL konsantrasyonlarında pozitif genotoksik etki göstermiştir.

Keywords

Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster

Abstract

This study aimed to determine the potential genotoxic effect of fluoxetine hydrochloride (FLX-HCl), an antidepressant commonly used for treating depression, using Somatic Mutation and Recombination Test (SMART). Third-¬instar Drosophila melanogaster larvae transheterozygous for the mutations multiple wing hair (mwh) and flare (flr3) were chronically fed in a medium containing different concentrations of FLX-HCl (0.1, 0.5, 1, and 2 mg/mL) in the experimental group. Distilled water, 0.1 mM ethyl methane sulfonate (EMS), and 2% dimethyl sulfoxide (DMSO) were used in negative, positive, and solvent control groups, respectively. The survival percentages were calculated by determining the number of individuals surviving when the larvae completed their development in the experimental and control groups. In all application groups, the wings of 40 individuals with both normal and serrate wing phenotypes were examined under a microscope, and genetic changes were evaluated by counting the mutant clones in the wings. The data obtained show that 1 and 2 mg/mL concentrations of FLX-HCl caused toxic effects in D. melanogaster individuals. Additionally, FLX-HCl showed a negative genotoxic effect at 0.1 mg/mL concentration, insignificant at 0.5 mg/mL concentration, and positive at 1 and 2 mg/mL concentrations in terms of total mutation evaluation and clone induction frequency in D. melanogaster individuals.

Keywords

References

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Details

Primary Language

English

Subjects

Zoology (Other)

Journal Section

Research Article

Authors

Selda Öz ^*
0000-0003-1883-3441
Türkiye

Zeynep Nur Sarıkaya
0009-0003-9343-8887
Türkiye

Özüm Larçın
0009-0000-3232-9140
Türkiye

Rabia Sarıkaya
0000-0001-9247-8973
Türkiye

Early Pub Date

January 21, 2024

Publication Date

April 1, 2024

Submission Date

June 7, 2023

Acceptance Date

September 27, 2023

Published in Issue

Year 2024 Volume: 27 Number: 2

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1310729

IZ

https://izlik.org/JA42CU36TR

Cite

RIS / Bibtex

APA

Öz, S., Sarıkaya, Z. N., Larçın, Ö., & Sarıkaya, R. (2024). Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(2), 316-324. https://doi.org/10.18016/ksutarimdoga.vi.1310729

AMA

1.Öz S, Sarıkaya ZN, Larçın Ö, Sarıkaya R. Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster. KSU J. Agric Nat. 2024;27(2):316-324. doi:10.18016/ksutarimdoga.vi.1310729

Chicago

Öz, Selda, Zeynep Nur Sarıkaya, Özüm Larçın, and Rabia Sarıkaya. 2024. “Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila Melanogaster”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi 27 (2): 316-24. https://doi.org/10.18016/ksutarimdoga.vi.1310729.

EndNote

Öz S, Sarıkaya ZN, Larçın Ö, Sarıkaya R (April 1, 2024) Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 27 2 316–324.

IEEE

[1]S. Öz, Z. N. Sarıkaya, Ö. Larçın, and R. Sarıkaya, “Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster”, KSU J. Agric Nat., vol. 27, no. 2, pp. 316–324, Apr. 2024, doi: 10.18016/ksutarimdoga.vi.1310729.

ISNAD

Öz, Selda - Sarıkaya, Zeynep Nur - Larçın, Özüm - Sarıkaya, Rabia. “Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila Melanogaster”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 27/2 (April 1, 2024): 316-324. https://doi.org/10.18016/ksutarimdoga.vi.1310729.

JAMA

1.Öz S, Sarıkaya ZN, Larçın Ö, Sarıkaya R. Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster. KSU J. Agric Nat. 2024;27:316–324.

MLA

Öz, Selda, et al. “Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila Melanogaster”. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, vol. 27, no. 2, Apr. 2024, pp. 316-24, doi:10.18016/ksutarimdoga.vi.1310729.

Vancouver

1.Selda Öz, Zeynep Nur Sarıkaya, Özüm Larçın, Rabia Sarıkaya. Investigation of the Genotoxic Effect of Fluoxetine Hydrochloride in Drosophila melanogaster. KSU J. Agric Nat. 2024 Apr. 1;27(2):316-24. doi:10.18016/ksutarimdoga.vi.1310729

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