Exploring the Potential of Psephellus huber-Marathi (Wagenitz) Wagenitz: A Comprehensive UHPLC-MS/MS Analysis of Phytochemical Composition and Evaluation of Antioxidant, Antimicrobial, and Antiproliferative Activities

Tuğrul Çağrı Akman; Samed Şimşek; Zeynep Akşit; Ali Aydın; Mustafa Abdullah Yılmaz

doi:10.18016/ksutarimdoga.vi.1339345

EN TR

Exploring the Potential of Psephellus huber-Marathi (Wagenitz) Wagenitz: A Comprehensive UHPLC-MS/MS Analysis of Phytochemical Composition and Evaluation of Antioxidant, Antimicrobial, and Antiproliferative Activities

An Erratum to this article was published on December 31, 2024. http://dergipark.org.tr/en/pub/ksutarimdoga/article/1594909

Abstract

Psephellus huber-morathii (PH) is an endemic species in the Eastern Anatolia Region. In this study, besides the biological activities of the 70% methanol extract of PH, its comprehensive phytochemical composition was investigated. The phenolic composition of the PH extract was analyzed using the UHPLC-MS/MS method. To evaluate its antimicrobial characteristics, the microdilution technique was employed. The antioxidant capabilities and total phenolic and flavonoid contents of the extract were determined using the spectrophotometer. Additionally, the effects of the extracts on cell proliferation and NCI-60 survival parameter values were assessed using the MTT assay. Quinic acid and chlorogenic acid were major compounds. The extract showed high antioxidant activity in DPPH (IC50:13.9±0.4 μg mL-1) and FRAP (61.3±2.3 mg TE g extract-1) methods. The total phenolic and flavonoid contents of the extract were found as 52.2±1.9 mg GAE g extract-1 and 28.6±0.9 mg QE g extract-1, respectively. Discussion: The extract showed low antiproliferative activity against normal lung and retinal cell lines, promising anticancer effects on gynecological and colon cancer cells, and moderate antimicrobial activity against Bacillus cereus and Enterococcus faecium. The study demonstrated the medicinal potential and value of PH extract against infectious diseases and cancer.

Keywords

Psephellus huber-Marathi (Wagenitz) Wagenitz'in Potansiyelini Keşfetme: Fitokimyasal Bileşiminin Kapsamlı Bir UHPLC-MS/MS Analizi ve Antioksidan, Antimikrobiyal ve Antiproliferatif Aktivitelerinin Değerlendirmesi

Bu makale için 31 Aralık 2024 tarihinde bir düzeltme yayımlandı. http://dergipark.org.tr/en/pub/ksutarimdoga/article/1594909

Öz

Psephellus huber-morathii (PH), Doğu Anadolu Bölgesi'nde endemik bir türdür. Bu çalışmada PH'nin %70 metanol ekstraktının biyolojik aktivitelerinin yanı sıra kapsamlı fitokimyasal bileşimi de incelenmiştir. PH ekstraktının fenolik bileşimi, UHPLC-MS/MS yöntemi kullanılarak analiz edildi. Antimikrobiyal özelliklerini değerlendirmek için mikrodilüsyon tekniği kullanıldı. Ekstrenin antioksidan özellikleri, toplam fenolik ve flavonoit içerikleri spektrofotometre kullanılarak belirlendi. Ek olarak, ekstraktların hücre proliferasyonu ve NCI-60 sağkalım parametre değerleri üzerindeki etkileri, MTT deneyi kullanılarak değerlendirildi. Kinik asit ve klorojenik asit majör bileşiklerdi. Ekstrakt, DPPH (IC50:13.9±0.4 μg mL-1) ve FRAP (61.3±2.3 mg TE g ekstrakt-1) yöntemlerinde yüksek antioksidan aktivite göstermiştir. Ekstraktın toplam fenolik ve flavonoid içerikleri sırasıyla 52.2±1.9 mg GAE g ekstrakt-1 ve 28.6±0.9 mg QE g ekstrakt-1 olarak bulundu. Ekstrakt, normal akciğer ve retina hücre hatlarına karşı düşük antiproliferatif aktivite, jinokolojik ve kolon kanser hücreleri üzerinde umut verici bir antikanser etki ve Bacillus cereus ve Enterococcus faecium'a karşı orta derecede antimikrobiyal aktivite gösterdi. Çalışma, PH ekstraktının bulaşıcı hastalıklara ve kansere karşı tıbbi potansiyelini ve değerini göstermiştir.

Anahtar Kelimeler

Thanks

The authors thank Dr. Hüseyin AKŞİT and Prof. Dr. Ali Kandemir for the collection and taxonomic description of the plant samples.

References

Aksit, H., Aksit, Z., Simsek, S., Aydin, A., Yilmaz, M. A., Kandemir, A., & Koksal, E. (2022). LC-MS/MS profiling phytochemical content of Echinophora chrysantha (Apiaceae) and antiproliferative, antioxidant activity. Pharmacy & Pharmacology International Journal, 10(5), 190–194. https://doi.org/10.15406/PPIJ.2022.10.00384
Altundağ, E., Altundağ, B. G. E., & Gürdal, B. (2013). Anatomical characteristics of Centaurea glastifolia L. (Asteraceae) used as folk medicine in East Anatolia. Journal of Faculty of Pharmacy of Istanbul University, 40, 57-64. Retrieved from https://dergipark.org.tr/en/pub/iujfp/issue/578/5676
Arif, R., Küpeli, E., & Ergun, F. (2004). The biological activity of Centaurea l. species (Review). Fen Bilimleri Dergisi, 17(4), 149–164.
Aydin, Ö., Çoşkunçelebi, K., Gültepe, M., & Güzel, M. E. (2013). A contribution to the taxonomy of Centaurea including Psephellus (Asteraceae) based on anatomical and molecular data. Turkish Journal of Botany, 37(3), 419–427. https://doi.org/10.3906/ bot-1204-25
Barrero, A. F., Herrador, M. M., Arteaga, P., Cabrera, E., Rodriguez-Garcia, I., Garcia-Moreno, M., & Gravalos, D. G. (1997). Cytotoxic activity of flavonoids from Carthamus arborescens, Ononis natrix ssp. ramosissima and Centaurea malacitana. Fitoterapia (Milano), 68(3), 281–283.
Başer, K. H. C., Özek, G., Özek, T., & Duran, A. (2006). Composition of the essential oil of Centaurea huber-Marathi Wagenitz isolated from seeds by micro distillation. Flavor and Fragrance Journal, 21(3), 568–570. https://doi.org/10.1002/FFJ.1620
Chanda, S., & Nagani, K. (2013). In vitro and in vivo methods for anticancer activity evaluation and some Indian medicinal plants possessing anticancer properties: An Overview. Journal of Pharmacognosy and Phytochemistry, 2(2). Retrieved from www.phytojournal.com www.phy tojournal.com
Demiroz, T., Nalbantsoy, A., Kose, F. A., & Baykan, S. (2020). Phytochemical composition and antioxidant, cytotoxic, and anti-inflammatory properties of Psephellus goeksunensis (Aytaç & H. Duman) Greuter & Raab-Straube. South African Journal of Botany, 130, 1–7. https://doi.org/10.1016/ J.SAJB. 2019.11.021

Elshikh, M., Ahmed, S., Funston, S., Dunlop, P., McGaw, M., Marchant, R., & Banat, I. M. (2016). Resazurin-based 96-well plate microdilution method for the determination of the minimum inhibitory concentration of biosurfactants. Biotechnology Letters, 38(6), 1015–1019. https:// doi.org/10.1007/S10529-016-2079-2/ TABLES/3
Farrag, N., Abdel-Aziz, E., El-Domiaty, M., & El Shafea, A. (1993). Phytochemical investigation of Centaurea araneosa growing in Egypt. Zagazig Journal of Pharmaceutical Sciences, 2(1), 29–45. https://doi.org/10.21608/ZJPS.1993.188986
Güçlü, G., Ergül, M., Uçar, E., Eruygur, N., Ataş, M., & Aşkın AKPULAT, H. (2022). Anticancer, Antioxidant, Antimicrobial and Enzyme Inhibitory Activities of Inula aucheriana. 2022 KSU J. Agric Nat, 25(5), 946–954. https://doi.org/10.18016/ ksutarimdoga.vi.985837
Güven, K., Çelik, S., & Uysal, I. (2005). Antimicrobial activity of Centaurea species. Pharmaceutical Biology, 43(1), 67–71. https://doi.org/10.1080/ 13880200590903390
Holetz, F. B., Pessini, G. L., Sanches, N. R., Cortez, D. A. G., Nakamura, C. V., & Dias Filho, B. P. (2002). Screening of some plants used in Brazilian folk medicine for the treatment of infectious diseases. Memórias Do Instituto Oswaldo Cruz, 97(7), 1027–1031. https://doi.org/10.1590/S0074-0276200 20007 00017
Khammar, A., & Djeddi, S. (2012). Pharmacological and Biological Properties of some Centaurea Species. European Journal of Scientific Research, 84(3), 398–416. Retrieved from http://www. European Journal of scientificresearch.com
Khan, N. A., Quereshi, S., Pandey, A., & Srivastava, A. (2009). Antibacterial Activity of Seed Extracts of Commercial and Wild Lathyrus Species. Turkish Journal of Biology, 33(2), 165–169. https://doi.org/ 10.3906/biy-0806-25
Korkmaz, N., Sener, S. O., Balturk, N., Kanbolat, S., Badem, M., Aliyazicioglu, R., Ozgen, U., Kandemir, A., Karaoglu, S. A. (2019). Determination of Phenolic Contents by HPLC, Antioxidant, Antimicrobial, Antityrosinase, and Anticholinesterase Activities of Psephellus huber-marathon. Journal Of Pharmaceutical Research International, 26(1), 1–10. https://doi.org/10.9734/ JPRI/2019/V26I130125
Orallo, F., Lamela, M., Camiña, M., Uriarte, E., & Calleja, J. M. (1998). Preliminary study of the potential vasodilator effects on rat aorta of centaurein and centaureidin, two flavonoids from Centaurea corcubionensis. Planta Medica, 64(2), 116–119. https://doi.org/10.1055/S-2006-957386/ BIB
Özcan, M. (2013). Morphological and anatomical aspects of Psephellus pulcherrimus (syn: Centaurea pulcherrima var. freynii) (Cardueae, Asteraceae) growing in Turkey. Journal of Forestry Faculty, 14(1), 104-112.
Polatoglu, K., Sen, A., Bulut, G., Bitis, L., & Gören, N. (2015). The essential oil composition of Centaurea stenolepis Kerner. from Turkey. Http://Dx.Doi.Org/ 10.1080/0972060X.2014.935090, 17(6), 1268–1278. https://doi.org/10.1080/ 0972060X.2014.935090
Ríos, J. L., & Recio, M. C. (2005). Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology, 100(1–2), 80–84. https:// doi.org/10.1016/J.JEP.2005.04.025
Sarker, S. D., Shoeb, M., Celik, S., Jaspars, M., Nahar, L., Kong-Thoo-Lin, P., & MacManus, S. M. (2007). Extracts of Centaurea bornmuelleri and Centaurea huber-morathii inhibit the growth of colon cancer cells in vitro. Advances in Traditional Medicine, 7(4), 336–340. https://doi.org/10.3742/OPEM. 2007.7.4.336
Sarker, S. D., Kumarasamy, Y., Shoeb, M., Celik, S., Eucel, E., Middleton, M., & Nahar, L. (2005). Antibacterial and antioxidant activities of three Turkish species of the genus Centaurea. Advances in Traditional Medicine, 5(3), 246–250. https://doi.org/10.3742/OPEM.2005.5.3.246
Shoeb, A., MacManus, S., Jaspars, M., Nahar, L., Kong-Thoo-Lin, P., Celik, S., & Sarker, S. (2007). Lignans and Flavonoids from the Seeds of Centaurea bornmuelleri Hausskn. Ex. Bornm. and Centaurea huber-morathii Wagenitz. Polish Journal of Chemistry, 81(1), 39–44. https://doi.org/ 10.2/JQUERY.MIN.JS
Sipahi, H., Orak, D., Reis, R., Yalman, K., Şenol, O., Palabiyik-Yücelik, S. S., … Yesilada, E. (2022). A comprehensive study to evaluate the wound healing potential of okra (Abelmoschus esculentus) fruit. Journal of Ethnopharmacology, 287, 114843. https://doi.org/10.1016/J.JEP.2021.114843
Tarhan, L., Ozturk Urek, R., Oner, A., & Nakiboglu, M. (2022). Evaluation of phenolic profiles, antioxidant activities, and cytotoxic and apoptotic potentials of Phlomis angustissima and Phlomis fruticosa, medicinal plants from Turkey. European Journal of Integrative Medicine, 55, 102188. https://doi.org/10.1016/J.EUJIM.2022.102188
Yilmaz, M. A. (2020). Simultaneous quantitative screening of 53 phytochemicals in 33 species of medicinal and aromatic plants: A detailed, robust and comprehensive LC-MS/MS method validation. Industrial Crops and Products, 149, 112347. https://doi.org/10.1016/J.INDCROP.2020.112347
Zengin, G., Cakmak, Y. S., Guler, G. O., & Aktumsek, A. (2010). In vitro antioxidant capacities and fatty acid compositions of three Centaurea species collected from the Central Anatolia region of Turkey. Food and Chemical Toxicology, 48(10), 2638–2641. https://doi.org/10.1016/J.FCT.2010.06. 033

Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences (Other)

Journal Section

Research Article

Authors

Tuğrul Çağrı Akman ^*
0000-0002-5439-950X
Türkiye

Samed Şimşek
0000-0001-8451-3425
Türkiye

Zeynep Akşit
0000-0002-0349-0223
Türkiye

Ali Aydın
0000-0002-9550-9111
Türkiye

Mustafa Abdullah Yılmaz
0000-0002-4090-7227
Türkiye

Early Pub Date

April 23, 2024

Publication Date

August 15, 2024

Submission Date

August 8, 2023

Acceptance Date

December 19, 2023

Published in Issue

Year 2024 Volume: 27 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1339345

IZ

https://izlik.org/JA23MP99NA

Cite

RIS / Bibtex

APA

Akman, T. Ç., Şimşek, S., Akşit, Z., Aydın, A., & Yılmaz, M. A. (2024). Exploring the Potential of Psephellus huber-Marathi (Wagenitz) Wagenitz: A Comprehensive UHPLC-MS/MS Analysis of Phytochemical Composition and Evaluation of Antioxidant, Antimicrobial, and Antiproliferative Activities. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(4), 782-792. https://doi.org/10.18016/ksutarimdoga.vi.1339345