In vitro Determination of Salt Stress Responses of OH×F 333 and OH×F 97 Pear Clonal Rootstocks

Elif Uyduran; Bekir Şan

doi:10.18016/ksutarimdoga.vi.1354060

TR EN

OH×F 333 ve OH×F 97 Armut Klon Anaçlarının Tuz Stresine Tepkilerinin in vitro Koşullarda Belirlenmesi

Öz

Bu çalışmada OH×F 333 ve OH×F 97 armut klon anaçlarının tuz stresine gösterdiği tepkiler araştırılmıştır. Bu amaçla OH×F 333 ve OH×F 97 armut klon anaçlarının in vitro bitkileri kademeli olarak artırılan farklı konsantrasyonlarda tuz (0, 50, 100, 150 ve 200 mM NaCl) içeren ½ MS ortamında kültüre alınmıştır. Denemede in vitro koşullarda tuz konsantrasyonları arttıkça, rejenerasyon oranı ve sürgün sayısı değerleri azalmış, zararlanma derecesi ise önemli derecede artmıştır. 200 mM NaCl uygulamasında çoğu sürgünün canlılıklarını yitirdikleri tespit edilmiştir. Farklı tuz konsantrasyonlarının biyokimyasal parametreler üzerindeki etkisi incelendiğinde toplam fenolik madde, toplam flavonoid madde, prolin ve çözünebilir protein içeriklerinin azaldığı, lipid peroksidasyon içeriğinin ise tuz konsantrasyonlarına paralel şekilde arttığı belirlenmiştir. Ancak toplam fenolik ve flavonoid madde içeriklerinde 150 mM NaCl uygulamasında tekrar bir yükselişin olduğu tespit edilmiştir.

Anahtar Kelimeler

In vitro Determination of Salt Stress Responses of OH×F 333 and OH×F 97 Pear Clonal Rootstocks

Abstract

In this study, the responses of OH×F 333 and OH×F 97 pear clonal rootstocks to salt stress were investigated. For this purpose, in vitro, plants of OH×F 333 and OH×F 97 pear clonal rootstocks were cultured in ½ MS medium containing different concentrations of salt (0, 50, 100, 150, and 200 mM NaCl). To adapt the plants to salt stress, the doses of NaCl added to the medium were gradually increased at weekly intervals. In the experiment, in parallel with the increasing salt stress, the regeneration rate and shoot number values decreased, while the degree of damage increased significantly. It was determined that most of the shoots died in the application of 200 mM NaCl. When the effects of different salt concentrations on biochemical parameters were examined, it was determined that total phenolics, total flavonoids, proline, and soluble protein contents decreased, while lipid peroxidation increased in parallel with salt concentrations. However, it was determined that there was an increase in the total phenolic and flavonoid contents in 150 mM NaCl application.

Keywords

Supporting Institution

Isparta Uygulamalı Bilimler Üniversitesi, Bilimsel Araştırma Projeleri Yönetim Birimi

Project Number

2021-YL1-0123

Ethical Statement

Araştırma etik kurul

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Details

Primary Language

English

Subjects

Pomology and Treatment

Journal Section

Research Article

Authors

Elif Uyduran
0009-0003-3781-2205
Türkiye

Bekir Şan ^*
0000-0001-6483-8433
Türkiye

Early Pub Date

April 23, 2024

Publication Date

August 15, 2024

Submission Date

September 1, 2023

Acceptance Date

December 3, 2023

Published in Issue

Year 2024 Volume: 27 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1354060

IZ

https://izlik.org/JA69GR29YH

Cite

RIS / Bibtex

APA

Uyduran, E., & Şan, B. (2024). In vitro Determination of Salt Stress Responses of OH×F 333 and OH×F 97 Pear Clonal Rootstocks. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(4), 817-827. https://doi.org/10.18016/ksutarimdoga.vi.1354060