The Effect of Nettle (Urtica dioica L.), Carob (Ceratonia siliqua L.), and Chaste (Vitex agnus-castus L.) Plants on Fertility in Caenorhabditis elegans

Handan Saraç; Hülya Özpınar

doi:10.18016/ksutarimdoga.vi.1357294

EN TR

The Effect of Nettle (Urtica dioica L.), Carob (Ceratonia siliqua L.), and Chaste (Vitex agnus-castus L.) Plants on Fertility in Caenorhabditis elegans

Abstract

This study was carried out to determine the effects of different concentrations of water extract of Nettle (Urtica dioica L.), Carob (Ceratonia siliqua L.), and Chaste (Vitex agnus-castus L.) plants, which are traditionally used in the treatment of infertility among the people, on fertility in Caenorhabditis elegans (C. elegans), which is a model organism. Scope of work; 0.1%, 0.05%, 0.02%, and 0.01% concentrations of the water extracts of the plants were applied to the C. elegans standard medium and egg counts were made for 3 days according to the Koelle protocol. In addition, one day after each egg count, uncracked eggs were determined, and egg productivity was calculated. The analyses were performed in triplicate, the averages were determined and the differences between the groups were statistically compared with the SPSS program. According to the results obtained from the study, it was determined that Nettle, Carob, and Chaste plants have positive effects on fertility. Among the plants, it was determined that the plant that affected fertility the most was Nettle, followed by Chaste and Carob plants, respectively. In addition, in terms of dosage applications, the best effect in all three plants has been observed at the highest dose of 0.1%.

Keywords

Isırgan (Urtica dioica L.), Keçiboynuzu (Ceratonia siliqua L.) ve Hayıt (Vitex agnus-castus L.) Bitkilerinin Caenorhabditis elegans' ta Fertiliteye Etkisi

Öz

Bu çalışma, halk arasında geleneksel olarak infertilite tedavisinde kullanıldığı bilinen Isırgan (Urtica dioica L.), Keçiboynuzu (Ceratonia siliqua L.) ve Hayıt (Vitex agnus-castus L.) bitkilerinin su ekstraktının farklı konsantrasyonlarının, bir model organizma olan Caenorhabditis elegans (C. elegans)' ta fertilite üzerine olan etkilerini belirlemek amacıyla planlanmıştır. Çalışma kapsamında; bitkilerin su ekstraktlarının %0.1, %0.05, %0.02 ve %0.01’lik konsantrasyonları C. elegans standart besiyerine uygulanmış ve Koelle protokolüne göre 3 gün boyunca yumurta sayımı yapılmıştır. Ayrıca, her yumurta sayımı yapıldıktan bir gün sonra çatlamayan yumurtalar tespit edilerek, yumurta verimlilikleri de hesaplanmıştır. Analizler üç tekrarlı olarak yapılmıştır ve ortalamalar belirlenerek SPSS programı ile gruplar arasındaki farklar istatistiksel olarak karşılaştırılmıştır. Çalışmadan elde edilen sonuçlara göre, Isırgan, Keçiboynuzu ve Hayıt bitkilerinin fertilite üzerinde olumlu etkilere sahip olduğu belirlenmiştir. Bitkiler arasında fertiliteye en fazla etki eden bitkinin Isıgan olduğu bunu sırasıyla Hayıt ve Keçiboynuzu bitkilerinin takip ettiği belirlenmiştir. Ayrıca, doz uygulamalarında her üç bitkide de benzer şekilde en iyi etki en yüksek doz olan %0.1’ lik uygulamada tespit edilmiştir.

Anahtar Kelimeler

Supporting Institution

Sivas Cumhuriyet Üniversitesi Bilimsel Araştırma Projeleri (CÜBAP)

Project Number

SMYO-028

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Details

Primary Language

English

Subjects

Pharmaceutical Botany

Journal Section

Research Article

Authors

Handan Saraç ^*
0000-0001-7481-7978
Türkiye

Hülya Özpınar
0000-0001-8154-0874
Türkiye

Early Pub Date

April 23, 2024

Publication Date

August 15, 2024

Submission Date

September 13, 2023

Acceptance Date

January 5, 2024

Published in Issue

Year 2024 Volume: 27 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1357294

IZ

https://izlik.org/JA72TG88CJ

Cite

RIS / Bibtex

APA

Saraç, H., & Özpınar, H. (2024). The Effect of Nettle (Urtica dioica L.), Carob (Ceratonia siliqua L.), and Chaste (Vitex agnus-castus L.) Plants on Fertility in Caenorhabditis elegans. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(4), 758-766. https://doi.org/10.18016/ksutarimdoga.vi.1357294

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