Determination of The Potential of Some Weeds to be Used as An Indicator Plant in Studies On Plumpox virus-T

Cemile Temur Çınar; Elif Dertli; Yasin Kaya; Doğan Işık

doi:10.18016/ksutarimdoga.vi.1360250

EN TR

Determination of The Potential of Some Weeds to be Used as An Indicator Plant in Studies On Plumpox virus-T

Abstract

Sharka is the most destructive viral disease of Prunus species caused by the plum pox virus (PPV). A unique strain of PPV has been identified from Turkey and named as PPV-Turkey (PPV-T). Being obligate parasitic organisms, viruses cannot be cultured in artificial nutrient media. Weeds play a significant role in virus ecology and epidemiology as they serve as alternative hosts for plant viruses and food for virus vectors. This study investigated the indicator potential of some weeds (such as Chenopodium album and Amaranthus retroflexus) for PPV-T. C. album and A. retroflexus plants were inoculated by dusting three leaves on each 3-week-old plant. A homogeneous group of 10 plants grown singly in pots was inoculated with PPV-T. Mock- and non-inoculated plants were used as negative controls. Inoculated plants were monitored daily for symptom development. The PPV-T inoculated leaves were tested by reverse transcription-polymerase chain reaction (RT-PCR) following the appearance of symptoms. The results of the indicator test revealed that C. album (6 out of 10 plants) and A. retroflexus (10 plants) reacted with the infection. Initially, this indicator reacted with local symptoms, and later symptoms of systemic infection occurred.

Keywords

Bazı Yabancı Otların Plum Pox Virüs T ile Ilgili Çalışmalarda Indikatör Bitki Olarak Kullanım Potansiyelinin Belirlenmesi

Öz

Plum pox virus (PPV)’ün neden olduğu şarka hastalığı, sert çekirdekli meyvelerin en önemli ve en yıkıcı viral hastalığıdır. PPV, Türkiye'de sert çekirdekli meyve yetiştiriciliği yapılan bazı bölgelerdeki bahçelerde oldukça yaygındır ve ciddi bir sorun teşkil etmektedir. Ülkemize özgü bir ırk olan PPV-T (Turkey) ilk olarak kayısı, şeftali ve erikte ağaçlarında tespit edilmiştir. Virüsler obligat parazit organizmalar oldukları için yapay besin ortamlarda kültüre alınamazlar. Bu nedenle deneysel ortamda çalışma yapabilmek için canlı bir konukçuya ihtiyaç vardır. Yabancı otlar bitki virüs hastalıkları açısından değerlendirildiğinde virüslerin ve vektörlerinin doğal yada alternatif konukçusu olabilir ki buda yabancı otların virüs epidemiyolojisinin doğal bir unsuru olduğunu göstermektedir. Bu projede Türkiye’nin yerli ırkı kabul edilen PPV-T ile ilgili araştırmalarda kullanılabilecek en uygun indikatör yabancı ot konukçunun belirlenmesi amaçlanmıştır. İndikatör bitki olarak kullanılacak Chenopodium album ve Amaranthus retroflexus yabancı otlarının üç yaprağına PPV-T mekanik inokülasyonla uygulanmıştır. Her bir uygulama için 10 bitki kullanılmıştır. PPV-T inokulasyonu yapılmamış bitkiler kontrol bitkisi olarak kullanılmıştır. Simtom gelişimini takiben inokülasyon yapılmış ve kontrol bitkileri RT-PCR testi ile analiz yapılmıştır. Kullanılan üç bitkinin ikisinde simptomolojik gözlemler ve RT-PCR sonucunda PPV-T inokulasyonunun başarılı olduğu belirlenmiştir (C. album (10 bitkiden 6'sı) ve A. retroflexus (10 bitkinin tamamı).

Anahtar Kelimeler

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Details

Primary Language

English

Subjects

Phytopathology , Herbology

Journal Section

Research Article

Authors

Cemile Temur Çınar ^*
0000-0002-0248-1835
Türkiye

Elif Dertli
0000-0001-6612-3546
Türkiye

Yasin Kaya
0009-0001-3025-8120
Türkiye

Doğan Işık
0000-0002-0554-2912
Türkiye

Early Pub Date

July 2, 2024

Publication Date

September 17, 2024

Submission Date

September 14, 2023

Acceptance Date

February 14, 2024

Published in Issue

Year 2024 Volume: 27 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1360250

IZ

https://izlik.org/JA45YX64HH

Cite

RIS / Bibtex

APA

Temur Çınar, C., Dertli, E., Kaya, Y., & Işık, D. (2024). Determination of The Potential of Some Weeds to be Used as An Indicator Plant in Studies On Plumpox virus-T. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(5), 1087-1094. https://doi.org/10.18016/ksutarimdoga.vi.1360250