Anti-biofilm, Anti-quorum sensing, Anti-swarming, and Antimicrobial Activity of Rubus fruticosus L.

Ülkü Zeynep Üreyen Esertaş; İnci Durukan; Ali Osman Kılıç; Saliha Ekşi

doi:10.18016/ksutarimdoga.vi.1396393

TR EN

Rubus fruticosus L. nin Anti-biyofilm, Anti-quorum sensing, Anti-swarming ve Antimikrobiyal Aktivitesi

Öz

Son on yılda dünya çapında benzeri görülmemiş bir oranda artan antibiyotik direnci sağlık sistemleri için ciddi bir zorluk oluşturmaktadır. Araştırmalar, bu direnç sorununun özellikle 2050'li yıllarda büyük can kayıplarına yol açacağını öngörmektedir. Dirençle mücadelede etkili antibiyotiklerin yerini alabilecek alternatif yöntemler araştırılmaktadır. Rubus fruticosus L., böğürtlen adıyla bilinen ve meyvesiyle ünlü bir çalı bitkisidir. Bu meyvenin tıbbi, kozmetik ve besin değeri oldukça yüksektir. Çalışmamızda, Rize iline ait Rubus fruticosus meyvesinin metanol, etil asetat, etanol ve hekzan ekstraktları antimikrobiyal ve antiquorum sensing aktivite açısından incelenmiştir. Çeşitli Gram-negatif ve Gram-pozitif bakterilere ve iki Candida cinsine ait türe karşı antimikrobiyal aktivite, agar difüzyon yöntemi ile araştırılmıştır. Anti-quorum sensing ve antibiofilm aktiviteleri ise Chromobacterium violaceum ATCC 12472 ve Pseudomonas aeruginosa PAO1 kullanılarak değerlendirilmiştir. Sonuçlar, Rubus fruticosus’un metanol ekstraktının antimikrobiyal aktiviteye sahip olduğunu, etanol ekstraktının ise antibiofilm ve antiswarming aktivitelerine sahip olduğunu göstermektedir. Bu bulgular, Rubus fruticosus’un antimikrobiyal dirençle mücadelede potansiyel bir doğal ajan olarak kullanılabileceğini düşündürmektedir.

Anahtar Kelimeler

Anti-biofilm, Anti-quorum sensing, Anti-swarming, and Antimicrobial Activity of Rubus fruticosus L.

Abstract

Over the past decade, antibiotic resistance has increased at an unprecedented rate, posing a serious challenge to healthcare systems worldwide. Research indicates that this resistance issue, which is projected to cause significant loss of life by the 2050s, is particularly alarming. Consequently, alternative methods to effective antibiotics are being explored to combat resistance. Rubus fruticosus L., commonly known as blackberry, is a shrub plant famous for its fruit. This fruit holds significant medicinal, cosmetic, and nutritional value. In our study, methanol, ethyl acetate, ethanol, and hexane extracts of Rubus fruticosus fruit from the Rize region were screened for their antimicrobial and quorum-sensing activities. Antimicrobial activity was investigated using the agar diffusion method against various Gram-negative and Gram-positive bacteria, as well as two Candida species. Anti-quorum sensing and antibiofilm activities were evaluated using Chromobacterium violaceum ATCC 12472 and Pseudomonas aeruginosa PAO1. The results showed that the methanol extract of Rubus fruticosus exhibited antimicrobial activity, while the ethanol extract demonstrated antibiofilm and anti-swarming activities. These findings suggest that Rubus fruticosus has the potential to be used as a natural agent in combating antimicrobial resistance.

Keywords

Supporting Institution

Recep Tayyip Erdoğan Üniversitesi

Project Number

TDK-2018-959

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Details

Primary Language

English

Subjects

Microbiology (Other)

Journal Section

Research Article

Authors

Ülkü Zeynep Üreyen Esertaş ^*
0000-0001-9897-5313
Türkiye

İnci Durukan
0000-0002-9789-4738
Türkiye

Ali Osman Kılıç
0000-0002-5506-0866
Türkiye

Saliha Ekşi
0000-0002-8818-7855
Türkiye

Early Pub Date

June 10, 2025

Publication Date

July 4, 2025

Submission Date

November 26, 2023

Acceptance Date

May 7, 2025

Published in Issue

Year 2025 Volume: 28 Number: 4

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1396393

IZ

https://izlik.org/JA65JS79AF

Cite

RIS / Bibtex

APA

Üreyen Esertaş, Ü. Z., Durukan, İ., Kılıç, A. O., & Ekşi, S. (2025). Anti-biofilm, Anti-quorum sensing, Anti-swarming, and Antimicrobial Activity of Rubus fruticosus L. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(4), 903-913. https://doi.org/10.18016/ksutarimdoga.vi.1396393

Cited By

Anticancer, Antibiofilm, Antimicrobial Effects and Anti-Quorum Sensing Inhibition Potentials of Alchemilla oriturcica and Helichrysum armenium Extracts

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

https://doi.org/10.18016/ksutarimdoga.vi.1511359