Chemical Composition and Antimicrobial Effect of Essential Oil of Anthemis pauciloba Boiss. var. pauciloba from Türkiye

Damla Kırcı; Ayla Kaya; Betül Demirci; Süleyman Doğu

doi:10.18016/ksutarimdoga.vi.1402876

TR EN

Türkiye'den Anthemis pauciloba Boiss. var. pauciloba'nın Uçucu Yağının Kimyasal Bileşimi ve Antimikrobiyal Etkisi

Öz

Anthemis pauciloba Boiss. var. pauciloba, Türkiye’de tanımlanan dört varyeteden biridir ve halk arasında “bol papatya” olarak bilinmektedir. Bitkinin çiçekleri, astım tedavisinde soğuk çay şeklinde kullanılmaktadır. Araştırmanın amacı, A. pauciloba var. pauciloba'nın toprak üstü kısımlarından Clevenger tipi aparat kullanılarak hidrodistilasyon yöntemiyle elde edilen uçucu yağın (EO) kimyasal bileşimini belirlemektir. Uçucu yağın bileşenleri, GC-FID ve GC-MS teknikleriyle eşzamanlı olarak analiz edilmiştir. Uçucu yağının mikrobiyal suşlara karşı antibakteriyel ve antifungal aktivitesini mikrodilüsyon tekniği kullanarak değerlendirmiştir. Uçucu yağın ana bileşikleri olarak α-tuyon (%28.7), α-pinen (%26.7) ve β-tuyon (%9.0) bulunmuştur. Gram-negatif, gram-pozitif ve mayaya karşı antimikrobiyal aktivite (Minimum İnhibitör Konsantrasyon) değerlendirilmiştir. Uçucu yağ, Candida krusei'ye karşı en yüksek antimikrobiyal aktiviteyi göstermiştir (MİK: 1.25 mg/mL). Bu çalışmada, A. pauciloba var. pauciloba’nın toprak üstü kısımlarından elde edilen uçucu yağın antimikrobiyal aktivitesi ilk kez değerlendirilmiştir.

Anahtar Kelimeler

Chemical Composition and Antimicrobial Effect of Essential Oil of Anthemis pauciloba Boiss. var. pauciloba from Türkiye

Abstract

Anthemis pauciloba Boiss. var. pauciloba is one of four recognized varieties in Türkiye, locally known as “bol papatya.” Its flowers are traditionally used as a cold infusion to treat asthma. The aim of the research was to determine the chemical composition of essential oil (EO) of A. pauciloba var. pauciloba aerial parts obtained by hydrodistillation using a Clevenger-type apparatus, examined by GC-FID, and GC-MS, simultaneously. The EO was evaluated for antibacterial and antifungal activities against microbial strains utilizing the broth-microdilution technique. α-Thujone (28.7%), α-pinene (26.7%), and β-thujone (9.0%) were found as the main constituents of EO. The antimicrobial activity (Minimum Inhibitory Concentration) against gram-negative, gram-positive, and yeast was observed by the essential oil. The essential oil demonstrated the highest antimicrobial activity against Candida krusei (MIC: 1.25 mg/mL). The antimicrobial activity of the essential oil from the aerial parts of A. pauciloba var. pauciloba was evaluated for the first time in this study.

Keywords

References

Babushok, V. I., Linstrom, P. J., & Zenkevich, I. G. (2011). Retention indices for frequently reported compounds of plant essential oils. Journal of Physical and Chemical Reference Data, 40(1), 1–47. https://doi.org/ 10.1063/1.3653552
Bassolé, I. H. N., & Juliani, H. R. (2012). Essential oils in combination and their antimicrobial properties. Molecules 17(4), 3989–4006. https://doi.org/10.3390/molecules17043989
Bizimbitkiler. https://bizimbitkiler.org.tr/yeni/demos/technical. (Alınma Tarihi: 13.12.2024).
Bulatovic, V. M., Menkovic, N. R., Vajs, V. E., Milosavijevic, S. M. & Djokovic, D. D. (1997). Essential oil of Anthemis carpatica. Journal of Essential Oil Research 9(4), 397-400. https://doi.org/10.1080/ 10412905.1997.9700739
Bulatovic, V. M., Menkovic, N. R., Vajs, V. E., Milosavijevic, S. M. & Djokovic, D. D. (1998). Essential oil of Anthemis montana. Journal of Essential Oil Research 10(2), 223-226. https://doi.org/10.1080/10412905. 1998.9700887
Burt, S. (2004). Essential oils: Their antibacterial properties and potential applications in foods—A review. International Journal of Food Microbiology 94(3), 223–253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
CLSI (NCCLS) M7-A7 (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, seventh edition, Wayne, USA.
Davis, P. H. (1975). Flora of Türkiye and the East Aegean Islands, Vol. 5. Edinburgh, UK: Edinburgh University Press.

Demirci, B., Kırcı, D., Öztürk, G., & Demirci, F. (2022). Effect of Extraction Time on Origanum onites L. Infusions and Essential Oils–Biological Evaluation, Statistical Principal Component and Hierarchial Cluster Analyses. Chemistry & Biodiversity 19(12), e202200482. https://doi.org/10.1002/cbdv.202200482
Demirci, F., Güven, K., Demirci, B., Dadandi, M. Y., & Baser, K. H. C. (2008). Antibacterial activity of two Phlomis essential oils against food pathogens. Food control 19(12), 1159-1164. https://doi.org/10.1016/j.foodcont.2008.01.001
Formisano, C., Rigano, D., Senatore, F., Raimondo, F. M., Maggio, A. & Bruno, M. (2012). Essential oil composition and antibacterial activity of Anthemis mixta and A. tomentosa (Asteraceae). Natural Product Communications 7(10), 1934578X1200701035. https://doi.org/10.1177/1934578X1200701035
Güner, A., Özhatay, N., Ekim, T. & Baser, K. H. C. (2000). Flora of Türkiye and the East Aegean Islands, Vol. 11, University Press, Edinburgh.
Hamzaoğulu, E., Budak, Ü. & Koç, M.A. (2011). new taxon of Anthemis L. (Asteraceae) from Türkiye: Anthemis pauciloba Boiss. var. alba Hamzaoğlu & Budak var. nova. Turkish Journal of Botany 35, 85-88. https://doi.org/10.3906/bot-1003-15
Keskin, E. B., Servi, H. & Çelik, S. (2017). Essential oil composition and fatty acid profile of Cota tinctoria subsp. euxina (Boiss.) Oberpr. & Greuter and endemic Anthemis pauciloba Boiss. var. sieheana (Eig) Grierson from Türkiye. Natural Volatiles & Essential Oils 4(3), 112-113.
Kürkçüoğlu, M., Dualı, G., Duran, A. & Başer, K. H. C. (2009). The essential oil composition of Anthemis pauciloba 80155. var. microstephana (eig.) Grierson and Anthemis pauciloba var. pauciloba (Poster bildiri). 8th International Symposium on the Chemistry of Natural Compounds, Eskisehir, Türkiye, 15-17 June 2009, ss.152.
Kurtulmus, A., Fafal, T., Mert, T., Saglam, H., Kivcak, B., Ozturk, T., Demirci, B. & Baser, K. H. C. (2009). Chemical composition and antimicrobial activity of the essential oils of three Anthemis species from Türkiye. Chemistry of Natural Compounds 45, 900-904.
Melikoğlu, G., Kurtoğlu, S. & Kültür, Ş. (2015). Türkiye’de astım tedavisinde geleneksel olarak kullanılan bitkiler. Marmara Pharmaceutical Journal 19, 1-11. https://doi.org/10.12991/mpj.2015198604
Mighri, H., Hajlaoui, H., Akrout, A., Najjaa, H. & Neffati, M. (2010). Antimicrobial and antioxidant activities of Artemisia herba-alba essential oil cultivated in Tunisian arid zone. Comptes Rendus Chimie 13(3), 380-386. https://doi.org/10.1016/j.crci.2009.09.008
NIST Chemistry WebBook. https://webbook.nist.gov/(Alınma Tarihi: 27.03.2025).
Orlando, G., Zengin, G., Ferrante, C., Ronci, M., Recinella, L., Senkardes, I., Gevrenova, R., Zheleva-Dimitrova, D., Chiavaroli, A., Leone, S., Simone, S., Brunetti, L., Picot-Allain, C. M. N., Mahomoodally, M. F., Sinan, K. I. & Menghini, L. (2019). Comprehensive chemical profiling and multidirectional biological investigation of two wild Anthemis species (Anthemis tinctoria var. pallida and A. cretica subsp. tenuiloba): focus on neuroprotective effects. Molecules 24(14), 2582. https://doi.org/10.3390/molecules24142582
Özbek, M. F., Duman, H., Özbek, F. & Aytaç, Z. (2021). Anthemis ekicii (Asteraceae), a new species from Türkiye. Turkish Journal of Botany 45, 59-68. https://doi.org/10.3906/bot-2009-38
Pavlović, M., Lakušić, D., Kovačević, N., Tzakou, O. & Couladis, M. (2010). Comparative analysis of essential oils of six Anthemis taxa from Serbia and Montenegro. Chemistry & Biodiversity 7(5), 1231-1244. https://doi.org/10.1002/cbdv.200900156
Pelkonen, O., Abass, K. & Wiesner, J. (2013). Thujone and thujone-containing herbal medicinal and botanical products: Toxicological assessment. Regulatory Toxicology and Pharmacology 65(1), 100-107. https://doi.org/10.1016/j.yrtph.2012.11.002
The pherobase. http://www.pherobase.com/database/kovats/kovatsdetailsulcatone.php (Alınma Tarihi: 27.03.2025).
Puchem. https://pubchem.ncbi.nlm.nih.gov/compound/Isocaryophyllene-oxide#section=Computed-Properties (Alınma Tarihi: 27.03.2025).
Radulović, N. S., Genčić, M. S., Stojanović, N. M., Randjelović, P. J., Stojanović-Radić, Z. Z. & Stojiljković, N. I. (2017). Toxic essential oils. Part V: Behaviour modulating and toxic properties of thujones and thujone-containing essential oils of Salvia officinalis L., Artemisia absinthium L., Thuja occidentalis L. and Tanacetum vulgare L. Food and Chemical Toxicology 105, 355-369. https://doi.org/10.1016/j.fct.2017.04.044
Saltan, N., Kırcı, D., Köse, Y.B. & Demirci, B. (2018). Chemical compositions and antimicrobial activity of Prunella vulgaris L. International Journal of Agriculture Environment and Food Sciences 2(1), 186-189. https://doi.org/10.31015/jaefs.18032
Saroglou, V., Dorizas, N., Kypriotakis, Z. & Skaltsa, H. D. (2006). Analysis of the essential oil composition of eight Anthemis species from Greece. Journal of Chromatography A 1104, 313–322. https://doi.org/10.1016/ j.chroma.2005.11.087
Zámboriné Németh, É. & Thi Nguyen, H. (2020). Thujone, a widely debated volatile compound: What do we know about it? Phytochemistry Reviews 19, 405-423. https://doi.org/10.1007/s11101-020-09671-y

Details

Primary Language

English

Subjects

Pharmaceutical Botany

Journal Section

Research Article

Authors

Damla Kırcı ^*
0000-0002-3479-3999
Türkiye

Ayla Kaya
0000-0002-7598-7132
Türkiye

Betül Demirci
0000-0003-2343-746X
Türkiye

Süleyman Doğu
0000-0002-5352-9288
Türkiye

Early Pub Date

May 1, 2025

Publication Date

June 9, 2025

Submission Date

December 29, 2024

Acceptance Date

April 17, 2025

Published in Issue

Year 2025 Volume: 28 Number: 3

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1402876

IZ

https://izlik.org/JA65FL82JL

Cite

RIS / Bibtex

APA

Kırcı, D., Kaya, A., Demirci, B., & Doğu, S. (2025). Chemical Composition and Antimicrobial Effect of Essential Oil of Anthemis pauciloba Boiss. var. pauciloba from Türkiye. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 28(3), 617-624. https://doi.org/10.18016/ksutarimdoga.vi.1402876