Investigation (In-Vitro) of Antiproliferative Properties of Pseudopediastrum boryanum (Turpin) E. Hegewald Extracts in Various Cancer Cell Lines

Tuğba Demiriz Yücer

doi:10.18016/ksutarimdoga.vi.1420650

EN TR

Investigation (In-Vitro) of Antiproliferative Properties of Pseudopediastrum boryanum (Turpin) E. Hegewald Extracts in Various Cancer Cell Lines

Abstract

In this study, the antiproliferative effects of Pseudopediastrum boryanum isolated and cultured from Tokat Yeşilırmak River benthic habitats were investigated with in-vitro experiments. The Anticancer activity of algae extracts was tested in cancer cell lines using the BrdU cell proliferation ELISA method. Vero (African Green Monkey Kidney Tumour Cells) and HeLa (Human Uterine Carcinoma Cells) were examined in concentrations of 100, 250 and 500 µg mL-1. As a result of antiproliferative tests, P. boryanum was found to have effective anti-cancer properties. P. boryanum extract concentrations of 250 and 500 µg mL-1 had a significant cytotoxic effect on Vero and HeLa cell lines after 24 hours. According to the results, P. boryanum is estimated to be a good option for quantitative pharmaceutical analyses and could be evaluated in the pharmaceutical industry.

Keywords

Pseudopediastrum boryanum (Turpin) E. Hegewald Ekstraktlarının Çeşitli Kanser Hücre Hatlarında Antiproliferatif Özelliklerinin (İn Vitro) Araştırılması

Öz

Bu çalışmada, Tokat Yeşilırmak Nehri bentik habitatlarından izole edilip kültürü yapılan Pseudopediastrum boryanum’un antiproliferatif etkileri, invitro deneylerle araştırıldı. Alg ekstraktlarının antikanser aktivitesi, BrdU hücre proliferasyon ELİSA yöntemi kullanılarak kanser hücre hatlarında denendi. Vero (Afrika Yeşil Maymun Böbrek Tümör Hücreleri) ve HeLa (İnsan Servikal Kanser Hücreleri) üzerinde 100, 250 ve 500 µg mL-1’ lik konsantrasyonlarda incelendi. Antiproliferatif testler sonucunda, P. boryanum’un, etkili antikanser özelliğe sahip olduğu görüldü. P. boryanum’un 250 ve 500 µg mL-1’ lik ekstrakt konsantrasyonlarının 24 saat sonunda Vero ve HeLa hücre hatları üzerinde, önemli derecede sitotoksik etki gösterdiği kaydedildi. Elde edilen sonuçlara göre, P. boryanum'un kantitatif farmasötik analizler için iyi bir aday olduğu ve ilaç endüstrisinde değerlendirilebileceği tahmin edilmektedir.

Anahtar Kelimeler

Thanks

The author thanks Professor doctor Köksal Pabuçcu for his help in the diagnosis of algae species.

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Details

Primary Language

English

Subjects

Microbiology (Other)

Journal Section

Research Article

Authors

Tuğba Demiriz Yücer ^*
0000-0002-2494-4511
Türkiye

Early Pub Date

July 2, 2024

Publication Date

September 17, 2024

Submission Date

January 16, 2024

Acceptance Date

March 28, 2024

Published in Issue

Year 2024 Volume: 27 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1420650

IZ

https://izlik.org/JA37UZ26FN

Cite

RIS / Bibtex

APA

Demiriz Yücer, T. (2024). Investigation (In-Vitro) of Antiproliferative Properties of Pseudopediastrum boryanum (Turpin) E. Hegewald Extracts in Various Cancer Cell Lines. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(5), 1026-1031. https://doi.org/10.18016/ksutarimdoga.vi.1420650