Biological activities of Elaeagnus umbellata methanol extract

Ülkü Zeynep Üreyen Esertaş; Merve Cora

doi:10.18016/ksutarimdoga.vi.1434448

TR EN

Elaeagnus umbellata metanol ekstraktının biyolojik aktivitesi

Öz

Asya ve Güney Avrupa’da yaygın olarak yetişen ve Sonbahar zeytini olarak bilinen Elaeagnus umbellata, kanser dahil olmak üzere birçok hastalığın geleneksel tedavisinde kullanılan bir çalı ağacıdır. Bu çalışmanın amacı E. umbellate ekstraktının çeşitli biyolojik etkilerinin belirlenmesidir. Ekstraktın 11 mikroorganizma üzerindeki aktivitesi agar kuyucuk difüzyon tekniği ile belirlendi. Ekstraktın anti-quorum sensing aktivitesi Chromobacterium violaceum ATCC 12472, anti-biyofilm ve anti-swarming aktiviteleri ise Pseudomonas aeruginosa PAO1 kullanılarak test edildi. Ekstraktın pankreatik tümöral hücre hattı (AR42J), meme kanseri hücre hattı (MDA-MB-231), akciğer adenokarsinoma hücre hattı (A549) ve normal epitel hücre hattı (Vero)’nasitotoksik etkisi ve herpes simpleks virüs tip-1’e karşı antiviral etkisi MTT yöntemi ile araştırıldı. Ekstraktın 6/11 mikroorganizmaya karşı orta düzeyde etkili olduğu ve anti-quorum sensing aktivite gösterdiği belirlendi. Ekstraktın kanser hücre hatlarında sitotoksk etkisi görülmezken, Vero hücrelerine 100 µg/mL ve üzeri konsantrasyonlarda sitotoksik etkisi olduğu tespit edildi. Bununla birlikte ekstraktın anti-biyofilm, anti-swarming ve antiviral aktivitesi görülmedi. Yapılan çalışma E. umbellata meyvesinin sınırlı biyolojik aktivitesinin olduğunu göstermektedir.

Anahtar Kelimeler

Biological activities of Elaeagnus umbellata methanol extract

Abstract

Elaeagnus umbellata, known as Autumn olive and growing widely in Asia and Southern Europe, is a shrub tree used in the traditional treatment of many diseases, including cancer. In this study, it was aimed to investigate the several biological effects of methanol extract of E. umbellate. The activity of the extract on 11 microorganisms was determined by the agar well diffusion technique. The anti-quorum sensing activity of the extract was tested using Chromobacterium violaceum ATCC 12472. The anti-biofilm and anti-swarming activities were tested using Pseudomonas aeruginosa PAO1. Cytotoxic effect of the extract against pancreatic tumoral cell line (AR42J), breast cancer cell line (MDA-MB-231), lung adenocarcinoma cell line (A549), and normal epithelial cell line (Vero), and antiviral effect of the extract against herpes simplex virus type 1 was analyzed using the MTT method. It was determined that the extract was moderately effective against 6/11 microorganisms and showed anti-quorum sensing activity. While the extract did not have a cytotoxic effect on cancer cell lines, it was found to have a cytotoxic effect on Vero cells at concentrations of 100 µg/mL and above. However, no anti-biofilm, anti-swarming, and antiviral activity of the extract was observed. The study shows that E. umbellata fruit has limited biological activity.

Keywords

References

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Details

Primary Language

English

Subjects

Microbiology (Other)

Journal Section

Research Article

Authors

Ülkü Zeynep Üreyen Esertaş ^*
0000-0001-9897-5313
Türkiye

Merve Cora
0000-0002-5956-9133
Türkiye

Early Pub Date

August 15, 2024

Publication Date

November 7, 2024

Submission Date

February 9, 2024

Acceptance Date

May 27, 2024

Published in Issue

Year 2024 Volume: 27 Number: 6

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1434448

IZ

https://izlik.org/JA23ES98CG

Cite

RIS / Bibtex

APA

Üreyen Esertaş, Ü. Z., & Cora, M. (2024). Biological activities of Elaeagnus umbellata methanol extract. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(6), 1262-1268. https://doi.org/10.18016/ksutarimdoga.vi.1434448

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