Detection of ‘Candidatus Phytoplasma mali’ and ‘Candidatus Phytoplasma pronotum’ in Apple Trees

Harun Meral; Muhammet Ekemen; Çiğdem Ulubaş Serçe

doi:10.18016/ksutarimdoga.vi.1440286

EN TR

Detection of ‘Candidatus Phytoplasma mali’ and ‘Candidatus Phytoplasma pronotum’ in Apple Trees

Abstract

The apple orchards in Niğde, Turkiye were surveyed for ‘Candidatus Phytoplasma mali’ associated with apple proliferation disease, and the suspicious samples were tested by PCR-RFLP methods. A comprehensive study was conducted which included sampling from a total of 19 orchards from four different districts. The samples were collected according to the major symptoms of phytoplasma disease which were foliar reddening, witches’ brooms, leaf rosettes, yellowing, longer peduncles and development of undersized fruits. It was determined that six out of 62 plant samples were infected with phytoplasma. Also, two out of six infected samples were determined as infected by ‘Ca. P. mali’ and unexpectedly four out of six infected samples were determined as infected by ‘Ca. P. prunorum’ is associated with European Stone Fruit Yellows disease. Even if the incidence of the disease (9.7%) was low, the characterized phytoplasmas were considered a significant potential threat for these locations.

Keywords

Elma ağaçlarında ‘Candidatus Phytoplasma mali’ ve ‘Candidatus Phytoplasma prunorum’ teşhisi

Öz

Niğde ilindeki eki elma bahçelerinde elma çoklu sürgün hastalığına neden olan 'Candidatus Phytoplasma mali' tespiti için survey yapılmıştır ve şüpheli örnekler PCR-RFLP yöntemiyle test edilmiştir. Dört farklı ilçedeki toplam 19 meyve bahçesinden örneklemeler yapılmıştır. Örnekler, fitoplazma hastalığının başlıca belirtileri olan yaprak kızarması, cadı süpürgesi, yaprak rozetleri, sararma, sapların uzaması ve meyvelerin cılız gelişmesi gibi belirtilere göre toplanmıştır. Toplam 62 bitki örneğinden altısının fitoplazma ile enfekte olduğu belirlendi. Ayrıca, enfekte olmuş altı örnekten ikisinin 'Ca. P. mali' ile enfekte olduğu ve beklenmedik bir şekilde enfekte olmuş altı örnekten dördünün Avrupa Sert Meyve Sarıları hastalığı ile ilişkili 'Ca P prunorum' tarafından enfekte olduğu belirlenmiştir. Hastalığın görülme sıklığı (%9,7) düşük olsa bile, karakterize edilen fitoplazmaların bu lokasyonlar için önemli bir potansiyel tehdit olduğu değerlendirilmiştir.

Anahtar Kelimeler

References

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Details

Primary Language

English

Subjects

Phytopathology

Journal Section

Research Article

Authors

Harun Meral
0000-0002-2621-6323
Türkiye

Muhammet Ekemen
0000-0003-4011-7302
Türkiye

Çiğdem Ulubaş Serçe ^*
0000-0001-5337-5883
Türkiye

Early Pub Date

July 2, 2024

Publication Date

September 17, 2024

Submission Date

February 20, 2024

Acceptance Date

April 7, 2024

Published in Issue

Year 2024 Volume: 27 Number: 5

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1440286

IZ

https://izlik.org/JA88YZ67TJ

Cite

RIS / Bibtex

APA

Meral, H., Ekemen, M., & Ulubaş Serçe, Ç. (2024). Detection of ‘Candidatus Phytoplasma mali’ and ‘Candidatus Phytoplasma pronotum’ in Apple Trees. Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(5), 1080-1086. https://doi.org/10.18016/ksutarimdoga.vi.1440286