Antioxidant, Antimicrobial, and Antialzheimer Activities of Tagetes patula (Asteraceae)

Mansur Seymen Seğmenoğlu; Oğuzhan Koçer; Mustafa Sevindik; Nuh Korkmaz; Mehmet Ali Yüzbaşıoğlu; İmran Uysal

doi:10.18016/ksutarimdoga.vi.1455020

EN TR

Antioxidant, Antimicrobial, and Antialzheimer Activities of Tagetes patula (Asteraceae)

Abstract

Plants are natural products used in the prevention and treatment of many diseases. In this study, antioxidant, antimicrobial, anticholinesterase activities and total phenolic and flavonoid contents of Tagetes patula L. samples collected from Iraq were determined. The aerial parts of the plant were extracted with ethanol in a soxhlet device. The antioxidant potential of the extracts was measured with Rel Assay kits. Antimicrobial activity was determined by the agar dilution method against standard bacterial and fungal strains. To determine anticholinesterase activity acetyl and butyrylcholinesterase inhibitions were tested. The total phenolic content of the samples was carried out using the Folin-Ciocalteu reagent. The quantification of flavonoids was conducted using an aluminum chloride assay. As a result of the analyses, the total antioxidant value of the plant extract was determined as 5.386±0.142 mmol Trolox equiv./L, the total oxidant value was 8.287±0.146 μmol H2O2 equiv./L and the oxidative stress index was determined as 0.154±0.003. Plant extracts showed the highest activity against Candida species. It was also effective against bacterial and fungal strains at concentrations between 50-400 µg/mL. Acetylcholinesterase activity of the plant extract was determined as 24.97±0.98, and butyrylcholinesterase activity was determined as 35.65±0.94. Additionally, its total phenolic content was determined as 63.64±0.74 mgGAE/g and its total flavonoid content was 108.9±1.55 mgQE/g. It has been determined that the plant has antioxidant, antimicrobial, and antiallergic potential.

Keywords

Tagetes patula'nın (Asteraceae) Antioksidan, Antimikrobiyal ve Antialzheimer aktiviteleri

Öz

Bitkiler birçok hastalığın önlenmesinde ve tedavisinde kullanılan doğal ürünlerdir. Bu çalışmada Tagetes patula’nın Irak’dan toplanan örneklerinin antioksidan, antimikrobiyal, antikolinesteraz aktiviteleri ve toplam fenolik ve flavonoid içerikleri belirlenmiştir. Bu kapsamda bitkinin toprak üstü kısmının etanol ile soxhlet cihazında özütleme işlemi yapıldı. Özütlerin antioksidan potansiyeli Rel Assay kitleri ile ölçüldü. Antimikrobiyal aktivite standart bakteri ve fungus suşlarına karşı agar dilüsyon metodu ile belirlendi. Antikolinesteraz aktivite için asetil ve bütirilkolinesteraz inhibisyonları test edildi. Numunelerin toplam fenolik içeriğinin miktarının belirlenmesi, Folin-Ciocalteu reaktifi kullanılarak gerçekleştirildi. Flavonoidlerin miktarının belirlenmesi, bir alüminyum klorür tahlili kullanılarak gerçekleştirildi. Yapılan analizler sonucunda bitki özütünün toplam antioksidan değeri 5.386±0.142 mmol Trolox equiv./L, toplam oksidan değeri 8.287±0.146 μmol H2O2 equiv./L ve oksidatif stress indeksi 0.154±0.003 olarak belirlendi. Bitki özütleri en yüksek aktiviteyi Candida türlerine karşı gösterdi. Ayrıca bakteri ve fungus suşlarına karşı 50-400 µg/mL arasındaki konsantrasyonlarda etkili olduğu tespit edildi. Bitki özütünün asetilkolinesteraz aktivitesi 24.97±0.98 μg/mL, bütirilkolinesteraz aktivitesi 35.65±0.94 μg/mL olarak belirlendi. Ayrıca toplam fenolik içeriği 63.64±0.74 mgGAE/g, toplam flavonoid içeriği 108.9±1.55 mgQE/g olarak tespit edildi. Bu kapsamda bitkinin antioksidan, antimikrobiyal ve Antialzheimer potansiyelinin olduğu tespit edilmiştir.

Anahtar Kelimeler

References

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Details

Primary Language

English

Subjects

Botany (Other)

Journal Section

Research Article

Authors

Mansur Seymen Seğmenoğlu
0000-0003-2743-6245
Türkiye

Oğuzhan Koçer
0000-0002-0104-7586
Türkiye

Mustafa Sevindik ^*
0000-0001-7223-2220
Türkiye

Nuh Korkmaz
0000-0001-8299-910X
Türkiye

Mehmet Ali Yüzbaşıoğlu
0000-0002-0245-751X
Türkiye

İmran Uysal
0000-0003-0942-9658
Türkiye

Early Pub Date

September 15, 2024

Publication Date

December 25, 2024

Submission Date

March 20, 2024

Acceptance Date

June 23, 2024

Published in Issue

Year 2024 Volume: 27 Number: Ek Sayı 1 (Suppl 1)

DOI

https://doi.org/10.18016/ksutarimdoga.vi.1455020

IZ

https://izlik.org/JA39ZG56XP

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RIS / Bibtex

APA

Seğmenoğlu, M. S., Koçer, O., Sevindik, M., Korkmaz, N., Yüzbaşıoğlu, M. A., & Uysal, İ. (2024). Antioxidant, Antimicrobial, and Antialzheimer Activities of Tagetes patula (Asteraceae). Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi, 27(Ek Sayı 1 (Suppl 1), 205-212. https://doi.org/10.18016/ksutarimdoga.vi.1455020

Antioxidant, Antimicrobial, and Antialzheimer Activities of Tagetes patula (Asteraceae)

Antioxidant, Antimicrobial, and Antialzheimer Activities of Tagetes patula (Asteraceae)

Abstract

Keywords

Tagetes patula'nın (Asteraceae) Antioksidan, Antimikrobiyal ve Antialzheimer aktiviteleri

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

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